ORCID Profile
0000-0003-1916-2758
Current Organisation
University of Adelaide
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Research Topics
In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Top 5 Research Topics
ANZSRC Field of Research (FoR)
Wine Chemistry and Wine Sensory Science | Horticultural Production | Plant Physiology | Oenology and Viticulture
ANZSRC Socio-Economic Objective (SEO)
Beverages (excl. Fruit Juices) | Expanding Knowledge in the Agricultural and Veterinary Sciences | Wine Grapes |
Related Links
Publications
Long-term drought adaptation of unirrigated grapevines (Vitis vinifera L.)
Publisher: Springer Science and Business Media LLC
Date: 30-04-2022
DOI: 10.1007/S40626-022-00243-3
Abstract: Resilience to drought stress is an important crop selection and breeding target particularly under an environment of increasing freshwater scarcities resulting from higher evapotranspiration rates and reduced precipitation. Identifying new genetic material and clones with superior drought tolerance would increase available genetic resources and germplasm for both grapevine breeders and propagators, providing genetic material that has greater water use efficiency thereby reducing reliance on supplemental irrigation. Towards this goal, we explored the long-term drought adaptation of field-grown, unirrigated (or dry-grown) and own-rooted grapevines of pre-clonal origin from shallow (SR) and deep (DR) soils representing low and high soil water availability, respectively, in a South Australian vineyard. Despite lower soil moisture available to SR vines, both deep and shallow-rooted vines had similar vine water status, based on measurements of midday stem water potential ( $${\\Psi }_{s}$$ Ψ s ), and leaf net photosynthesis ( A n ). Due to the lower stomatal conductance ( g s ), SR had higher intrinsic water use efficiency ( WUE i ) than DR, however the carbon isotope ratio ( $${\\delta }^{13}C$$ δ 13 C ) of the fruit at harvest was similar between the two groups. Our observations suggest a degree of drought adaptation in the SR vines resulting from multi-decadal cyclical droughts. Overall, we demonstrate that pre-clonal Cabernet Sauvignon grapevines dry-grown in shallow soils have an enhanced resilience to drought compared to dry-grown vines in deep soils. This study has implications for selection of crop genetic material in a changing climate.
Assessing the Feasibility of Using Sentinel-2 Imagery to Quantify the Impact of Heatwaves on Irrigated Vineyards
Publisher: MDPI AG
Date: 02-12-2019
DOI: 10.3390/RS11232869
Abstract: Heatwaves are common in many viticultural regions of Australia. We evaluated the potential of satellite-based remote sensing to detect the effects of high temperatures on grapevines in a South Australian vineyard over the 2016–2017 and 2017–2018 seasons. The study involved: (i) comparing the normalized difference vegetation index (NDVI) from medium- and high-resolution satellite images (ii) determining correlations between environmental conditions and vegetation indices (Vis) and (iii) identifying VIs that best indicate heatwave effects. Pearson’s correlation and Bland–Altman testing showed a significant agreement between the NDVI of high- and medium-resolution imagery (R = 0.74, estimated difference −0.093). The band and the VI most sensitive to changes in environmental conditions were 705 nm and enhanced vegetation index (EVI), both of which correlated with relative humidity (R = 0.65 and R = 0.62, respectively). Conversely, SWIR (short wave infrared, 1610 nm) exhibited a negative correlation with growing degree days (R = −0.64). The analysis of heat stress showed that green and red edge bands—the chlorophyll absorption ratio index (CARI) and transformed chlorophyll absorption ratio index (TCARI)—were negatively correlated with thermal environmental parameters such as air and soil temperature and growing degree days (GDDs). The red and red edge bands—the soil-adjusted vegetation index (SAVI) and CARI2—were correlated with relative humidity. To the best of our knowledge, this is the first study demonstrating the effectiveness of using medium-resolution imagery for the detection of heat stress on grapevines in irrigated vineyards.
Facilitating mapping and understanding of within‐vineyard variation in fruit composition using data pooled from multiple vineyards
Publisher: Hindawi Limited
Date: 28-03-2022
DOI: 10.1111/AJGW.12556
Use of Machine Learning with Fused Spectral Data for Prediction of Product Sensory Characteristics: The Case of Grape to Wine
Publisher: MDPI AG
Date: 09-02-2023
Abstract: Generations of sensors have been developed for predicting food sensory profiles to circumvent the use of a human sensory panel, but a technology that can rapidly predict a suite of sensory attributes from one spectral measurement remains unavailable. Using spectra from grape extracts, this novel study aimed to address this challenge by exploring the use of a machine learning algorithm, extreme gradient boosting (XGBoost), to predict twenty-two wine sensory attribute scores from five sensory stimuli: aroma, colour, taste, flavour, and mouthfeel. Two datasets were obtained from absorbance-transmission and fluorescence excitation-emission matrix (A-TEEM) spectroscopy with different fusion methods: variable-level data fusion of absorbance and fluorescence spectral fingerprints, and feature-level data fusion of A-TEEM and CIELAB datasets. The results for externally validated models showed slightly better performance using only A-TEEM data, predicting five out of twenty-two wine sensory attributes with R2 values above 0.7 and fifteen with R2 values above 0.5. Considering the complex biotransformation involved in processing grapes to wine, the ability to predict sensory properties based on underlying chemical composition in this way suggests that the approach could be more broadly applicable to the agri-food sector and other transformed foodstuffs to predict a product’s sensory characteristics from raw material spectral attributes.
Evaluating a novel microtensiometer for continuous trunk water potential measurements in field-grown irrigated grapevines
Publisher: Springer Science and Business Media LLC
Date: 12-11-2022
Dynamic aspects of plant water potential revealed by a microtensiometer
Publisher: Cold Spring Harbor Laboratory
Date: 24-06-2021
DOI: 10.1101/2021.06.23.449675
Abstract: Water potential is a fundamental thermodynamic parameter that describes the activity of water. In this paper, we describe the continuous measurement of plant water potential, a reliable indicator of its water status, using a novel in situ sensor known as a ‘microtensiometer’ in mature grapevines under field conditions. The microtensiometer operates on the principle of equilibration of water potentials of internal liquid water with an external vapour or liquid phase. We characterised the seasonal and diurnal dynamics of trunk water potentials (Ψ trunk ) obtained from microtensiometers installed in two grapevine cultivars, Shiraz and Cabernet Sauvignon, and compared these values to pressure chamber-derived stem (Ψ stem ) and leaf (Ψ leaf ) water potentials as well as leaf stomatal conductance. Diurnal patterns of Ψ trunk matched those of Ψ stem and Ψ leaf under low vapour pressure deficit (VPD) conditions, but erged under high VPD conditions. The highest diurnal values of Ψ trunk were observed shortly after dawn, while the lowest values were typically observed in the late afternoon. Differential responses of Ψ trunk to VPD were observed between cultivars, with Shiraz more sensitive than Cabernet to increasing VPD over long time scales, and both cultivars had a stronger VPD response than soil moisture response. On a diurnal basis, however, time cross correlation analysis revealed that Shiraz Ψ trunk lagged Cabernet Ψ trunk in response to changing VPD. Microtensiometers were shown to operate reliably under field conditions over several months. To be useful for irrigation scheduling of woody crops, new thresholds of Ψ trunk need to be developed.
Grape Heterogeneity Index: Assessment of Overall Grape Heterogeneity Using an Aggregation of Multiple Indicators
Publisher: MDPI AG
Date: 24-03-2023
Abstract: Uniform grape maturity can be sought by producers to minimise underripe and/or overripe proportions of fruit and limit any undesirable effects on wine quality. Considering that grape heterogeneity is a multifaceted phenomenon, a composite index summarising overall grape heterogeneity was developed to benefit vineyard management and harvest date decisions. A grape heterogeneity index (GHI) was constructed by aggregating the sum of absolute residuals multiplied by the range of values from measurements of total soluble solids, pH, fresh weight, total tannins, absorbance at 520 nm (red colour), 3-isobutyl-2-methoxypyrazine, and malic acid. Management of grape heterogeneity was also studied, using Cabernet Sauvignon grapes grown under four viticultural regimes (normal/low crop load, full/deficit irrigation) during the 2019/2020 and 2020/2021 seasons. Comparisons of GHI scores showed grape variability decreased throughout ripening in both vintages, then significantly increased at the harvest time point in 2020, but plateaued on s le dates nearing the harvest date in 2021. Irrigation and crop load had no effect on grape heterogeneity by the time of harvest in both vintages. Larger vine yield, leaf area index, and pruning weight significantly increased GHI score early in ripening, but no significant relationship was found at the time of harvest. Differences in the Ravaz index, normalised difference vegetation index, and soil electrical conductivity did not significantly change the GHI score.
Objective assessment of dried sultana grape quality using digital image analysis
Publisher: Hindawi Limited
Date: 04-12-2017
DOI: 10.1111/AJGW.12316
The feasibility of using a low-cost near-infrared, sensitive, consumer-grade digital camera mounted on a commercial UAV to assess Bambara groundnut yield
Publisher: Informa UK Limited
Date: 24-09-2021
A Review of Current and Potential Applications of Remote Sensing to Study the Water Status of Horticultural Crops
Publisher: MDPI AG
Date: 17-01-2020
Abstract: With increasingly advanced remote sensing systems, more accurate retrievals of crop water status are being made at the in idual crop level to aid in precision irrigation. This paper summarises the use of remote sensing for the estimation of water status in horticultural crops. The remote measurements of the water potential, soil moisture, evapotranspiration, canopy 3D structure, and vigour for water status estimation are presented in this comprehensive review. These parameters directly or indirectly provide estimates of crop water status, which is critically important for irrigation management in farms. The review is organised into four main sections: (i) remote sensing platforms (ii) the remote sensor suite (iii) techniques adopted for horticultural applications and indicators of water status and, (iv) case studies of the use of remote sensing in horticultural crops. Finally, the authors’ view is presented with regard to future prospects and research gaps in the estimation of the crop water status for precision irrigation.
Characterisation of internal oxygen concentration of strawberry (
Publisher: CSIRO Publishing
Date: 16-12-2022
DOI: 10.1071/FP21259
Abstract: Gas exchange mechanisms play crucial roles in maintaining fruit post-harvest quality in perishable fruit such as strawberry (Fragaria × ananassa Duch.) and blueberry (Vaccinium corymbosum L.). The internal oxygen concentration ([O2]) of strawberry and blueberry were measured using Clark-type oxygen sensing electrodes. The volume of intercellular voids in strawberry was obtained by micro-computed tomography (micro-CT). In both berries, internal [O2] was consistent and relatively high across measured tissues. The overall [O2] was well above the Michaelis constant (Km) for cytochrome c oxidase in both fruit and different from previously examined grape (Vitis vinifera L.) berry mesocarp with near zero minimum [O2]. In strawberry and blueberry, cell vitality was also maintained at full maturity in the mesocarp. Higher storage temperature (i.e. 20 vs 4°C) reduced internal [O2] of strawberry. Pedicel detachment in blueberry was associated with greater fruit dehydration and lower internal [O2] after short-term storage of 12 h. The results suggest that the intercellular voids of the fruit’s mesocarp provide an efficient gas exchange route for maintaining high fruit internal [O2] post-harvest.
Characterising spatio‐temporal variation in fruit composition for improved winegrowing management in California Cabernet Sauvignon
Publisher: Hindawi Limited
Date: 27-02-2022
DOI: 10.1111/AJGW.12542
Evaluating Remotely-Sensed Grapevine (Vitis vinifera L.) Water Stress Responses Across a Viticultural Region
Publisher: MDPI AG
Date: 25-10-2019
Abstract: The evolving spatial and temporal knowledge about vineyard performance through the use of remote sensing offers new perspectives for vine water status studies. This paper describes the application of aerial thermal imaging to evaluate vine water status to improve irrigation scheduling decisions, water use efficiency, and overall winegrape quality in the Coonawarra viticultural region of South Australia. Airborne infrared images were acquired during the 2016 and 2017 growing seasons in the region of Coonawarra, South Australia. Several thermal indices of crop water status (CWSI, Ig, (Tc-Ta)) were calculated that correlated with conventional soil and vine water status measures (Ψpd, Ψs, gs). CWSI and Ig could discriminate between the two cultivars used in this study, Cabernet Sauvignon (CAS) and Shiraz (SHI), as did the conventional water stress measures. The relationship between conventional vine water status measures appeared stronger with CWSI in the warmer and drier season (2016) compared to the cooler and wetter season (2017), where Ig and (Tc-Ta) showed stronger correlations. The study identified CWSI, Ig and (Tc-Ta) to be reliable indicators of vine water status under a variety of environmental conditions. This is the first study to report on high resolution vine water status at a regional scale in Australia using a combination of remote and direct sensing methods. This methodology is promising for aerial surveillance of vine water status across multiple blocks and cultivars to inform irrigation scheduling.
A comparison of root and shoot hydraulics, aquaporin expression and leaf gas exchange between two grapevine cultivars reveals differences in hydraulic control mediated by aquaporins
Publisher: Cold Spring Harbor Laboratory
Date: 23-11-2017
DOI: 10.1101/224212
Abstract: Hydraulics of plants that take different strategies of stomatal control under water stress are still relatively poorly understood. Here we explore how root and shoot hydraulics, gas exchange, aquaporin expression and abscisic acid (ABA) concentration in leaf xylem sap ([ABA] xylem ) may be involved and coordinated. A comparison in responses to mild water stress and ABA application was made between two cultivars of Vitis vinifera L. previously classified as isohydric (Grenache) and anisohydric (Syrah). Grenache showed stronger adjustments of leaf, plant, and root hydraulic conductances to decreased soil moisture and a steeper correlation of stomatal conductance ( g s ) to [ABA] xylem than Syrah resulting in greater conservation of soil moisture, but not necessarily more isohydric behaviour. Under well-watered conditions, changes in vapour pressure deficit (VPD) had a strong influence on g s in both cultivars with adjustments of leaf hydraulic conductance. Grenache was more sensitive to decreases in soil water availability compared to Syrah that rather responded to VPD. There were stronger correlations between plant hydraulic parameters and changes in aquaporin gene expression in leaves and roots of Grenache. Overall, the results reinforce the hypothesis that both hydraulic and chemical signals significantly contribute to the differences in water conservation behaviours of the two cultivars.
3D visualisation of voids in grapevine flowers and berries using X‐ray micro computed tomography
Publisher: Hindawi Limited
Date: 06-01-2021
DOI: 10.1111/AJGW.12480
Detecting Grapevine Virus Infections in Red and White Winegrape Canopies Using Proximal Hyperspectral Sensing
Publisher: MDPI AG
Date: 06-03-2023
DOI: 10.3390/S23052851
Abstract: Grapevine virus-associated disease such as grapevine leafroll disease (GLD) affects grapevine health worldwide. Current diagnostic methods are either highly costly (laboratory-based diagnostics) or can be unreliable (visual assessments). Hyperspectral sensing technology is capable of measuring leaf reflectance spectra that can be used for the non-destructive and rapid detection of plant diseases. The present study used proximal hyperspectral sensing to detect virus infection in Pinot Noir (red-berried winegrape cultivar) and Chardonnay (white-berried winegrape cultivar) grapevines. Spectral data were collected throughout the grape growing season at six timepoints per cultivar. Partial least squares-discriminant analysis (PLS-DA) was used to build a predictive model of the presence or absence of GLD. The temporal change of canopy spectral reflectance showed that the harvest timepoint had the best prediction result. Prediction accuracies of 96% and 76% were achieved for Pinot Noir and Chardonnay, respectively. Our results provide valuable information on the optimal time for GLD detection. This hyperspectral method can also be deployed on mobile platforms including ground-based vehicles and unmanned aerial vehicles (UAV) for large-scale disease surveillance in vineyards.
Virus Pathogens in Australian Vineyards with an Emphasis on Shiraz Disease
Publisher: MDPI AG
Date: 28-07-2020
DOI: 10.3390/V12080818
Abstract: Grapevine viruses are found throughout the viticultural world and have detrimental effects on vine productivity and grape and wine quality. This report provides a comprehensive and up-to-date review on grapevine viruses in Australia with a focus on “Shiraz Disease” (SD) and its two major associated viruses, grapevine virus A (GVA) and grapevine leafroll-associated virus 3 (GLRaV-3). Sensitive grapevine cultivars like Shiraz infected with GVA alone or with a co-infection of a leafroll virus, primarily GLRaV-3, show symptoms of SD leading to significant yield and quality reductions in Australia and in South Africa. Symptom descriptors for SD will be outlined and a phylogenetic tree will be presented indicating the SD-associated isolates of GVA in both countries belong to the same clade. Virus transmission, which occurs through infected propagation material, grafting, and naturally vectored by mealybugs and scale insects, will be discussed. Laboratory and field-based indexing will also be discussed along with management strategies including rogueing and replanting certified stock that decrease the incidence and spread of SD. Finally, we present several cases of SD incidence in South Australian vineyards and their effects on vine productivity. We conclude by offering strategies for virus detection and management that can be adopted by viticulturists. Novel technologies such as high throughput sequencing and remote sensing for virus detection will be outlined.
Plant Viral Disease Detection: From Molecular Diagnosis to Optical Sensing Technology—A Multidisciplinary Review
Publisher: MDPI AG
Date: 23-03-2022
DOI: 10.3390/RS14071542
Abstract: Plant viral diseases result in productivity and economic losses to agriculture, necessitating accurate detection for effective control. Lab-based molecular testing is the gold standard for providing reliable and accurate diagnostics however, these tests are expensive, time-consuming, and labour-intensive, especially at the field-scale with a large number of s les. Recent advances in optical remote sensing offer tremendous potential for non-destructive diagnostics of plant viral diseases at large spatial scales. This review provides an overview of traditional diagnostic methods followed by a comprehensive description of optical sensing technology, including camera systems, platforms, and spectral data analysis to detect plant viral diseases. The paper is organized along six multidisciplinary sections: (1) Impact of plant viral disease on plant physiology and consequent phenotypic changes, (2) direct diagnostic methods, (3) traditional indirect detection methods, (4) optical sensing technologies, (5) data processing techniques and modelling for disease detection, and (6) comparison of the costs. Finally, the current challenges and novel ideas of optical sensing for detecting plant viruses are discussed.
Stress-Induced Volatile Emissions and Signalling in Inter-Plant Communication
Publisher: MDPI AG
Date: 29-09-2022
Abstract: The sessile plant has developed mechanisms to survive the “rough and tumble” of its natural surroundings, aided by its evolved innate immune system. Precise perception and rapid response to stress stimuli confer a fitness edge to the plant against its competitors, guaranteeing greater chances of survival and productivity. Plants can “eavesdrop” on volatile chemical cues from their stressed neighbours and have adapted to use these airborne signals to prepare for impending danger without having to experience the actual stress themselves. The role of volatile organic compounds (VOCs) in plant–plant communication has gained significant attention over the past decade, particularly with regard to the potential of VOCs to prime non-stressed plants for more robust defence responses to future stress challenges. The ecological relevance of such interactions under various environmental stresses has been much debated, and there is a nascent understanding of the mechanisms involved. This review discusses the significance of VOC-mediated inter-plant interactions under both biotic and abiotic stresses and highlights the potential to manipulate outcomes in agricultural systems for sustainable crop protection via enhanced defence. The need to integrate physiological, biochemical, and molecular approaches in understanding the underlying mechanisms and signalling pathways involved in volatile signalling is emphasised.
Effect of grape heterogeneity on wine chemical composition and sensory attributes for Vitis vinifera cv. Cabernet Sauvignon
Publisher: Hindawi Limited
Date: 06-12-2021
DOI: 10.1111/AJGW.12469
Evidence of Bi-Directional Volatile-Mediated Communication between Drought-Stressed and Well-Watered Grapevines (Vitis vinifera L.)
Publisher: MDPI AG
Date: 28-06-2023
Abstract: The volatile-mediated interplay between stressed and non-stressed plants has been described in many studies involving both biotic and abiotic stresses as a one-way channel. However, very little is known about the molecular basis and mechanisms by which volatile organic compounds (VOCs) mediate plant communication between drought-stressed ‘emitter’ plants and non-stressed ‘receiver’ neighbours for the defence against impending stress challenges. Aiming to address this in grapevine, this study investigated the effect of two-way VOC exchange between stressed and non-stressed Vitis vinifera L. cv. Shiraz during drought and recovery using four treatments: isolated well-watered (WW) vines, isolated drought-stressed (DS) vines, and co-located DS ‘emitter’ and WW ‘receiver’ vines in a growth room. The results obtained from solid-phase microextraction (SPME) gas chromatography mass spectrometry (GC-MS) analysis showed a synchronised decline in α-pinene concentration in the co-located treatment vines and higher isoprene levels in the DS emitters compared to the isolated DS vines. Targeted gene expression analysis further identified the over-expression of a key gene, allene oxide synthase (AOS), in the jasmonic acid (JA) biosynthesis pathway during peak drought in the DS emitter. Transcript expression of chorismate synthase (CHORS) and α-pinene synthase (VvPNaPin1) showed similar trends in the DS emitter. The results suggest that isoprene and α-pinene may be interplant signalling molecules used by grapevine during drought. To the best of our knowledge, this is the first report of a bi-directional interaction in grapevine between the emitters and receivers under drought stress mediated by the JA and terpenoid biosynthesis pathways.
Estimation of Grapevine Crop Coefficient Using a Multispectral Camera on an Unmanned Aerial Vehicle
Publisher: MDPI AG
Date: 05-07-2021
DOI: 10.3390/RS13132639
Abstract: Crop water status and irrigation requirements are of great importance to the horticultural industry due to changing climatic conditions leading to high evaporative demands, drought and water scarcity in semi-arid and arid regions worldwide. Irrigation scheduling strategies based on evapotranspiration (ET), such as regulated deficit irrigation, requires the estimation of seasonal crop coefficients (kc). The ET-driven irrigation decisions for grapevines rely on the s ling of several kc values from each irrigation zone. Here, we present an unmanned aerial vehicle (UAV)-based technique to estimate kc at the single vine level in order to capture the spatial variability of water requirements in a commercial vineyard located in South Australia. A UAV carrying a multispectral sensor is used to extract the spectral, as well as the structural, information of Cabernet Sauvignon grapevines. The spectral and structural information, acquired at the various phenological stages of the vine through two seasons, is used to model kc using univariate (simple linear), multivariate (generalised linear and additive) and machine learning (convolution neural network and random forest) model frameworks. The structural information (e.g., canopy top view area) had the strongest correlation with kc throughout the season (p ≤ 0.001 Pearson R = 0.56), while the spectral indices (e.g., normalised indices) turned less-sensitive post véraison—the onset of ripening in grapes. Combining structural and spectral information improved the model’s performance. Among the investigated predictive models, the random forest predicted kc with the highest accuracy (R2: 0.675, root mean square error: 0.062, and mean absolute error: 0.047). This UAV-based approach improves the precision of irrigation by capturing the spatial variability of kc within a vineyard. Combined with an energy balance model, the water needs of a vineyard can be computed on a weekly or sub-weekly basis for precision irrigation. The UAV-based characterisation of kc can further enhance the water management and irrigation zoning by matching the infrastructure with the spatial variability of the irrigation demand.
Insights into Long-Term Acclimation Strategies of Grapevines (Vitis vinifera L.) in Response to Multi-Decadal Cyclical Drought
Publisher: MDPI AG
Date: 19-12-2022
Abstract: Changing climatic conditions across Australia’s viticulture regions is placing increasing pressure on resources such as water and energy for irrigation. Therefore, there is a pressing need to identify superior drought tolerant grapevine clones by exploring the extensive genetic ersity of early European clones in old vineyards. Previously, in a field trial, we identified drought-tolerant (DT) dry-farmed Cabernet Sauvignon clones that had higher intrinsic water use efficiency (WUEi) under prolonged soil moisture deficiency compared to drought-sensitive (DS) clones. To investigate whether the field-grown clones have been primed and confer the drought-tolerant phenotypes to their subsequent vegetative progenies, we evaluated the drought responses of DT and DS progenies under two sequential drought events in a glasshouse alongside progenies of commercial clones. The DT clonal progenies exhibited improved gas exchange, photosynthetic performance and WUEi under recurrent drought events relative to DS clonal progenies. Concentration of a natural priming agent, γ-amino butyric acid (GABA), was significantly higher in DT progenies relative to other progenies under drought. Although DT and commercial clones displayed similar drought acclimation responses, their underlying hydraulic, stomatal and photosynthetic regulatory mechanisms were quite distinct. Our study provides fundamental insights into potential intergenerational priming mechanisms in grapevine.
Drought resilience of long term dry-farmed grapevines (Vitis vinifera L.)
Publisher: Cold Spring Harbor Laboratory
Date: 09-04-2021
DOI: 10.1101/2021.04.07.438886
Abstract: We explored the long-term drought resilience of field-grown unirrigated (or dry-grown) grapevines of pre-clonal origin from shallow (SR) and deep (DR) soils representing low and high soil water availability, respectively, in a warm, Mediterranean climate. Despite lower soil moisture available to SR vines, both deep and shallow-rooted vines had similar vine water status, based on measurements of midday stem water potential (Ψ s ), and leaf net photosynthesis ( A n ). Due to the lower stomatal conductance ( g s ), SR had higher intrinsic water use efficiency ( WUE i ) than DR, however the carbon isotope ratio ( δ 13 C ) of the fruit at harvest was similar between the two groups. Our observations suggest a degree of drought adaptation in the SR vines resulting from multi-decadal cyclical droughts. Overall, we demonstrate that pre-clonal Cabernet Sauvignon grapevines dry-grown in shallow soils have an enhanced resilience to drought compared to dry-grown vines in deep soils. This study has implications for selection of crop genetic material in a changing climate.
Insights into Long-term Acclimation Strategies of Grapevines in Response to Multi-decadal Cyclical Drought
Publisher: Cold Spring Harbor Laboratory
Date: 05-05-2022
DOI: 10.1101/2022.05.05.490818
Abstract: The Australian wine industry is currently under pressure to sustain its profitability due to climate change. Therefore, there is a pressing need to explore grapevine genetic ersity and identify superior clones with improved drought resistance. We previously characterised more than 15,000 dry-farmed (for over 65 years) Cabernet Sauvignon clones in a vineyard and identified three drought-tolerant (DT) clones, which can maintain significantly higher intrinsic water use efficiency ( WUE i ) under limited soil moisture than drought-sensitive (DS) clones. To understand whether DT clones grown under multi-decadal cyclical drought can prime their vegetatively-propagated clonal progenies for future drought events, in this study, all DT and DS vegetative progenies were propagated with commercial clones in the glasshouse. Their physiological and molecular responses were investigated under well-watered and two recurrent drought (D1 and D2) conditions. We observed that concentration of a natural priming agent, γ-amino butyric acid (GABA), were significantly higher in all DT progenies relative to other progenies under drought. Both commercial and DT progenies exhibited improved gas exchange, photosynthetic performance and WUE i under recurrent drought events relative to DS progenies. Our results suggest that DT progenies have adapted to be in a “primed state” to withstand future drought events.
Water Stress Impacts on Grapevines (Vitis vinifera L.) in Hot Environments: Physiological and Spectral Responses
Publisher: MDPI AG
Date: 31-07-2022
Abstract: The projected increase in temperature and water scarcity represents a challenge for winegrowers due to changing climatic conditions. Although heat and drought often occur concurrently in nature, there is still little known about the effects of water stress (WS) on grapevines in hot environments. This study aimed to assess whether the grapevine’s physiological and spectral responses to WS in hot environments differ from those expected under lower temperatures. Therefore, we propose an integrated approach to assess the physiological, thermal, and spectral response of two grapevine varieties (Vitis vinifera L.), Grenache and Shiraz, to WS in a hot environment. In a controlled environment room (CER), we imposed high-temperature conditions (TMIN 30 °C–TMAX 40 °C) and compared the performance of well-watered (WW) and WS-ed potted own-rooted Shiraz and Grenache grapevines (SH_WW, SH_WS, GR_WW, and GR_WS, respectively). We monitored the vines’ physiological, spectral, and thermal trends from the stress imposition to the recovery after re-watering. Then, we performed a correlation analysis between the physiological parameters and the spectral and thermal vegetation indices (VIs). Finally, we looked for the best-fitting models to predict the physiological parameters based on the spectral VIs. The results showed that GR_WS was more negatively impacted than SH_WS in terms of net photosynthesis (Pn, GR-WS = 1.14 μmol·CO2 m−2·s−1 SH-WS = 3.64 μmol·CO2 m−2·s−1), leaf transpiration rate (E, GR-WS = 1.02 mmol·H2O m−2·s−1 SH-WS = 1.75 mmol·H2O m−2·s−1), and stomatal conductance (gs, GR-WS = 0.04 mol·H2O m−2·s−1 SH-WS = 0.11 mol·H2O m−2·s−1). The intrinsic water-use efficiency (WUEi = Pn/gs) of GR_WS (26.04 μmol·CO2 mol−1 H2O) was lower than SH_WS (34.23 μmol·CO2 mol−1 H2O) and comparable to that of SH_WW (26.31 μmol·CO2 mol−1 H2O). SH_WS was not unaffected by water stress except for E. After stress, Pn, gs, and E of GR_WS did not recover, as they were significantly lower than the other treatments. The correlation analysis showed that the anthocyanin Gitelson (AntGitelson) and the green normalised difference vegetation index (GNDVI) had significant negative correlations with stem water potential (Ψstem), Pn, gs, and E and positive correlation with WUEi. In contrast, the photochemical reflectance index (PRI), the water index (WI), and the normalised difference infrared index (NDII) showed an opposite trend. Finally, the crop water stress (CWSI) had significant negative correlations with the Ψstem in both varieties. Our findings help unravel the behaviour of vines under WS in hot environments and suggest instrumental approaches to help the winegrowers managing abiotic stress.
Uptake and Glycosylation of Smoke-Derived Volatile Phenols by Cabernet Sauvignon Grapes and Their Subsequent Fate during Winemaking
Publisher: MDPI AG
Date: 14-08-2020
DOI: 10.3390/MOLECULES25163720
Abstract: Wine made from grapes exposed to bushfire smoke can exhibit unpleasant smoky, ashy characters, which have been attributed to the presence of smoke-derived volatile phenols, in free or glycosylated forms. Here we report the uptake and glycosylation of volatile phenols by grapes following exposure of Cabernet Sauvignon vines to smoke, and their fate during winemaking. A significant delay was observed in the conversion of volatile phenols to their corresponding glycoconjugates, which suggests sequestration, the presence of intermediates within the glycosylation pathway and/or other volatile phenol storage forms. This finding has implications for industry in terms of detecting smoke-affected grapes following vineyard smoke exposure. The potential for an in-canopy sprinkler system to mitigate the uptake of smoke-derived volatile phenols by grapes, by spraying grapevines with water during smoke exposure, was also evaluated. While “misting” appeared to partially mitigate the uptake of volatile phenols by grapes during grapevine exposure to smoke, it did not readily influence the concentration of volatile phenols or the sensory perception of smoke taint in wine. Commercial sensors were used to monitor the concentration of smoke particulate matter (PM) during grapevine exposure to low and high density smoke. Similar PM profiles were observed, irrespective of smoke density, such that PM concentrations did not reflect the extent of smoke exposure by grapes or risk of taint in wine. The sensors could nevertheless be used to monitor the presence of smoke in vineyards during bushfires, and hence, the need for compositional analysis of grapes to quantify smoke taint marker compounds.
Evaluating the Spectral and Physiological Responses of Grapevines (Vitis vinifera L.) to Heat and Water Stresses under Different Vineyard Cooling and Irrigation Strategies
Publisher: MDPI AG
Date: 27-09-2021
Abstract: Heat stress (HS) and water stress (WS) pose severe threats to viticulture, and effective management solutions to counter their effects on grapevine performance must be examined. In this study, we evaluated the physiological and spectral responses of Vitis vinifera L. cv. Sauvignon blanc to in idual (HS) and combined (HS + WS) stress under four different cooling and irrigation strategies. The treatments were: standard drip irrigation (SI), extra drip irrigation (SI+), extra sprinklers irrigation (SPRI), and sustained deficit irrigation (SDI 50% of SI). Compared to the other treatments, in the early stages after the occurrence of HS, the vine water status of SPRI and SI+ improved, with high stomatal conductance (gs) (SPRI) and stem water potential (Ψstem SPRI and SI+). All the physiological indicators measured were significantly lower after the end of HS in the SDI treatment. We also identified the spectral response of grapevine to HS and combined HS and WS (resulting from SDI). Consistent with the physiological analysis, the proximal spectral responses of leaves identified SPRI and SI+ as putative cooling strategies to minimize vine HS. The vines undergoing combined stress (SDI) showed greenness amelioration 10 days after stress, as revealed by the greenness vegetation indices (VIs), i.e., Green Index (GI), Normalized Difference Greenness Vegetation Index (NDGI), and Visible Atmospherically Resistant Index (VARI). However, their physiological recovery was not achieved within this time, as shown by the Simple Ratio Index (SRI), Transformed Chlorophyll Absorption Ratio Index (TCARI), and TCARI/Optimized Soil-Adjusted Vegetation Index (TCARI/OSAVI). A three-step band selection process allowed the identification of the spectral traits’ responsive to HS and combined stress, i.e., 1336–1340 nm, 1967–1971 nm, and 600–604 nm.
Evaluating the Potential of High-Resolution Visible Remote Sensing to Detect Shiraz Disease in Grapevines
Publisher: Hindawi Limited
Date: 05-05-2023
DOI: 10.1155/2023/7376153
Abstract: Background and Aims. Shiraz disease (SD) is a viral disease associated with Grapevine virus A that causes significant yield loss in economically important grape cultivars in Australia such as Shiraz and Merlot. Current diagnostic methods are time-consuming and costly. This study evaluates an alternative methodology using visible remote sensing imagery to detect SD in Shiraz grapevines. Methods and Results. High-resolution visible remote sensing images were captured of Shiraz grapevines in two South Australian viticultural regions over two seasons. The projected leaf area (PLA) of in idual grapevines was estimated from the images. Virus-infected vines had significantly lower PLA than healthy vines in the early season but fewer difference after veraison. The lower PLA was only observed in grapevines coinfected with grapevine leafroll-associated viruses (GLRaVs) and Grapevine virus A (GVA). Shiraz vines infected with either GLRaVs or GVA had similar PLA to healthy vines. Conclusions. High-resolution RGB remote sensing technology has the potential to rapidly estimate SD infection in Shiraz grapevines. Our observations of shoot devigouration only in coinfected vines calls into question the etiology of SD. Further validation of the PLA technique incorporating different regions, seasons, cultivars, and combinations of viruses is needed for improving the robustness of the method. Significance of the Study. This preliminary study presents a new rapid and low-cost surveillance method to estimate SD infections in Shiraz vineyards, which could significantly lower the cost for growers who conduct on-ground SD visual assessments or lab-based tissue testing at the vineyard scale.
Related Organisations
Related Funding Activities
Industrial Transformation Training Centres - Grant ID: IC170100008
Start Date: 02-2018
End Date: 12-2024
Amount: $4,459,672.00
Funder: Australian Research Council
View Funded Activity