ORCID Profile
0000-0003-4208-8912
Current Organisations
Utrecht University
,
Queensland University of Technology
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In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Special Education and Disability | Building | Quantity Surveying | Education Systems | Secondary Education | Architectural Design
Expanding Knowledge in Built Environment and Design | Expanding Knowledge in Education | Learner and Learning Processes |
Publisher: Informa UK Limited
Date: 13-09-2016
Publisher: Cogitatio
Date: 09-04-2015
DOI: 10.17645/SI.V3I2.57
Abstract: In response to the ratification of the United Nations Convention of the Rights of People with Disabilities (CRPD), Australian housing industry leaders, supported by the Australian Government, committed to transform their practices voluntarily through the adoption of a national guideline, called Livable Housing Design. They set a target in 2010 that all new housing would be visitable by 2020. Research in this area suggests that the anticipated voluntary transformation is unrealistic and that mandatory regulation will be necessary for any lasting transformation to occur. It also suggests that the assumptions underpinning the Livable Housing Design agreement are unfounded. This paper reports on a study that problematised these assumptions. The study used eleven newly-constructed dwellings in three housing contexts in Brisbane, Australia. It sought to understand the logics-of-practice in providing, and not providing, visitable housing. By examining the specific details that make a dwelling visitable, and interpreting the accounts of builders, designers and developers, the study identified three logics-of-practice which challenged the assumptions underpinning the Livable Housing Design agreement: focus on the point of sale an aversion to change and deference to external regulators on matters of social inclusion. These were evident in all housing contexts indicating a dominant industry culture regardless of housing context or policy intention. The paper suggests that financial incentives for both the builder and the buyer, demonstration by industry leaders and, ultimately, national regulation is a possible pathway for the Livable Housing Design agreement to reach the 2020 goal. The paper concludes that the Australian Government has three options: to ignore its obligations under the CRPD to revisit the Livable Housing Design agreement in the hope that it works or to regulate the housing industry through the National Construction Code to ensure the 2020 target is reached.
Publisher: Common Ground Research Networks
Date: 2017
Publisher: Walter de Gruyter GmbH
Date: 2019
Abstract: This paper explores the educator experience and sense-making of design thinking pedagogy in the higher education context. Design thinking has become a pedagogical phenomenon in higher education due to its widespread relevance across many disciplines. Some studies discuss design thinking as a pedagogy in the educational context however, there is a lack of empirical research to understand the educator perspective on design thinking pedagogy. Three design thinking educators who have had more than fifteen years of teaching experience were interviewed to explore their experiences. The data from these in idual in-depth, semi-structured interviews were analysed employing Interpretative Phenomenological Analysis (IPA). One super-ordinate theme capability building for everyone , and four subordinate themes developing a participatory approach towards world issues developing an open, explorative attitude developing creative ability and developing an ethical mindset were identified. From these findings, the paper argues that design thinking educators have the basis for a pedagogical rationale that transcends disciplinary boundaries and provides common ground for collaboration and on-going development of design thinking pedagogy as an emerging field in education.
Publisher: Cold Spring Harbor Laboratory
Date: 11-10-2022
DOI: 10.1101/2022.10.10.511592
Abstract: Engineering expanded effector recognition in plant immune receptors is a promising prospect for generating new disease resistant crop varieties. However, modification of plant NLR receptors has proven challenging due to the lack of understanding of their context as part of complex immune systems. Here, we demonstrate a new avenue for NLR-mediated engineering that exploits the allelic ersity in the Pik NLR pair to allow for the generation of receptors with expanded recognition specificities, which would otherwise result in constitutive cell death. This work lays the foundation for the incorporation of new effector recognition motifs into the Pik system and advances the development of designer NLRs that can be tailored to specific secreted pathogen signatures. Engineering the plant immune system offers genetic solutions to mitigate crop diseases caused by erse agriculturally significant pathogens and pests. Modification of intracellular plant immune receptors of the nucleotide-binding leucine rich repeat (NLRs) superfamily for expanded recognition of pathogen virulence proteins (effectors) is a promising approach for engineering novel disease resistance. However, engineering can cause NLR autoactivation, resulting in constitutive defence responses that are deleterious to the plant. This may be due to plant NLRs associating in highly complex signalling networks that co-evolve together, and changes through breeding or genetic modification can generate incompatible combinations, resulting in autoimmune phenotypes. We have previously shown how alleles of the rice NLR pair Pik have differentially co-evolved, and how sensor/helper mismatching between non-co-evolved alleles triggers constitutive activation and cell death (De la Concepcion et al., 2021b). Here, we dissect incompatibility determinants in the Pik pair and found that HMA domains integrated in Pik-1 not only evolved to bind pathogen effectors but also likely co-evolved with other NLR domains to maintain immune homeostasis. This explains why changes in integrated domains can lead to autoactivation. We then used this knowledge to facilitate engineering of new effector recognition specificities overcoming initial autoimmune penalties. We show that by mismatching alleles of the rice sensor and helper NLRs Pik-1 and Pik-2, we can enable the integration of synthetic HMA domains with novel and enhanced recognition of an effector from the rice blast fungus. Taken together, our results reveal a new strategy for engineering NLRs, which has the potential to allow an expanded set of integrations and therefore new disease resistance specificities in plants.
Publisher: Springer Singapore
Date: 2019
Publisher: Informa UK Limited
Date: 25-12-2016
Publisher: Emerald
Date: 03-08-2007
DOI: 10.3316/QRJ0702022
Publisher: Emerald
Date: 28-03-2022
Abstract: Despite growing evidence of the impact of school facilities on wellbeing and educational outcomes, no attention has been given to understanding this impact in relation to the interrelationship of design and procurement and their combined effect. This paper aims to address this gap by presenting the outcomes of a study of the design rocurement relationship pre-opening and post-opening of schools. Qualitative case study methodology enabled in-depth exploration of six Australian Government schools procured through “public private partnerships” (PPP) or “design & construct” (D& C) and “design, bid, build” (DBB). Data collected through interviews with architects, education department officers, school principals and teachers were analysed thematically using techniques aligned with grounded theory methodology. The paper reports three key findings: pre-opening of schools, budget impacts design similarly for procurement across PPP and (D& C/DBB) case schools pre-opening of schools, prescriptive design impacts procurement similarly across PPPs and D& C/DBB schools post-opening of schools, procurement impacts design and school operation in different ways across PPP and D& C/DBB schools. These findings point to a fundamental finding that it is design and procurement together that impacts well-being and educational outcomes as experienced by principals and teachers. This research may be of practical value for education departments, architects, facility managers, school principals and teachers. This paper provides original evidence of the relationship between procurement and design and their combined impact on student well-being and educational outcomes.
Publisher: Cold Spring Harbor Laboratory
Date: 10-08-2021
DOI: 10.1101/2021.08.10.455798
Abstract: The ability to recombinantly produce target proteins is essential to many biochemical, structural, and biophysical assays that allow for interrogation of molecular mechanisms behind protein function. Purification and solubility tags are routinely used to maximise the yield and ease of protein expression and purification from E. coli . A major hurdle in high-throughput protein expression trials is the cloning required to produce multiple constructs with different solubility tags. Here we report a modification of the well-established pOPIN expression vector suite to be compatible with modular cloning via Type IIS restriction enzymes. This allows users to rapidly generate multiple constructs with any desired tag, introducing modularity in the system and delivering compatibility with other modular cloning vector systems, for ex le streamlining the process of moving between expression hosts. We demonstrate these constructs maintain the expression capability of the original pOPIN vector suite and can also be used to efficiently express and purify protein complexes, making these vectors an excellent resource for high-throughput protein expression trials. pOPIN-GG expression vectors allow for modular cloning enabling rapid screening of purification and solubility tags at no loss of expression compared to previous vectors. Cloning into the pOPIN-GG vectors can be performed from PCR products or from level 0 vectors containing the required parts. Several vectors with different resistances and origins of replication have been generated allowing the effective co-expression and purification of protein complexes. All pOPIN-GG vectors generated here are available on Addgene, as well as level 0 acceptors and tags.
Publisher: Emerald
Date: 15-06-2015
Abstract: – The purpose of this paper is to understand the difficulties in implementing models of housing, and to help address the lack of accessible and affordable private housing for people with disability in Australia. In responding to this aim, the study formulated an ecological map of housing models, which are examined in this paper in terms of their underlying assumptions of vulnerability. – The study involved explanation building, using a multiple case study approach, informed theoretically by an ecological framework. It included organisations, families and in iduals with disability. – For the purpose of this paper, the study revealed a direct relationship between the nature of the housing models proposed, and assumptions of vulnerability. In the context of the study findings, the paper suggests that attempts to address in idual housing needs are more likely to achieve a positive outcome when they are person driven, from a premise of ability rather than disability. Overall, it invites a “universalistic” way of conceptualising housing issues for people with disability that has international relevance. – This paper highlights how assumptions of vulnerability shape environmental responses, such as housing, for people with disability. – This paper is based on a study that reconciled a person-centred philosophy with an ecological appreciation of the external and internal factors impacting housing choice for people with disability.
Publisher: Springer Singapore
Date: 2019
Publisher: Springer Singapore
Date: 2019
Publisher: Informa UK Limited
Date: 05-07-2020
Publisher: Oxford University Press (OUP)
Date: 24-07-2023
Abstract: Engineering the plant immune system offers genetic solutions to mitigate crop diseases caused by erse agriculturally significant pathogens and pests. Modification of intracellular plant immune receptors of the nucleotide-binding leucine-rich repeat (NLR) receptor superfamily for expanded recognition of pathogen virulence proteins (effectors) is a promising approach for engineering disease resistance. However, engineering can cause NLR autoactivation, resulting in constitutive defense responses that are deleterious to the plant. This may be due to plant NLRs associating in highly complex signaling networks that coevolve together, and changes through breeding or genetic modification can generate incompatible combinations, resulting in autoimmune phenotypes. The sensor and helper NLRs of the rice (Oryza sativa) NLR pair Pik have coevolved, and mismatching between noncoevolved alleles triggers constitutive activation and cell death. This limits the extent to which protein modifications can be used to engineer pathogen recognition and enhance disease resistance mediated by these NLRs. Here, we dissected incompatibility determinants in the Pik pair in Nicotiana benthamiana and found that heavy metal–associated (HMA) domains integrated in Pik-1 not only evolved to bind pathogen effectors but also likely coevolved with other NLR domains to maintain immune homeostasis. This explains why changes in integrated domains can lead to autoactivation. We then used this knowledge to facilitate engineering of new effector recognition specificities, overcoming initial autoimmune penalties. We show that by mismatching alleles of the rice sensor and helper NLRs Pik-1 and Pik-2, we can enable the integration of synthetic domains with novel and enhanced recognition specificities. Taken together, our results reveal a strategy for engineering NLRs, which has the potential to allow an expanded set of integrations and therefore new disease resistance specificities in plants.
Publisher: Wiley
Date: 25-07-2023
DOI: 10.1002/COL.22881
Abstract: Over the last four decades, development in the environmental color design field challenged the prevailing designer's attitude towards selecting architectural and urban color palettes. Examining praxiological issues in environmental color design is necessary to reveal influential conditions that can facilitate or obstruct a shift in the design paradigm. This research article presents a qualitative study of environmental color design praxis (ECDP) in urban contexts. The study sought to understand how designers constructed their perspectives on contemporary ECDP, how these perspectives influenced their design approaches and the conditions under which designers can change their attitude and practices in environmental color design. The conceptual model of ECDP emerged from the grounded theory analysis of the interviews with Brisbane designers and the interpretation of relevant texts written by prominent designers and scholars. This article describes the core components of the ECDP model and provides interpretations of how educational, pragmatic and socio‐psychological factors influence dynamic changes in ECDP. The underlying research concludes that a holistic understanding of ECDP can inform the advanced and socially responsive environmental color design paradigm the ECDP model provides a frame of reference for developing color design praxis theory.
Start Date: 12-2016
End Date: 12-2021
Amount: $391,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 09-2022
End Date: 08-2025
Amount: $363,854.00
Funder: Australian Research Council
View Funded Activity