ORCID Profile
0000-0003-1025-6520
Current Organisations
Macquarie University
,
Children's Hospital at Westmead
,
The University of Sydney School of Medicine
,
Royal Australasian College of Physicians
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Publications
A prospective comparative pilot study comparing the urine collection pad with clean catch urine technique in non-toilet-trained children
Publisher: Elsevier BV
Date: 04-2014
DOI: 10.1016/J.IENJ.2013.08.004
Abstract: There are many different methods for collecting urine from paediatric patients in emergency departments. Therefore, the aims of the study were to: The three month study was a prospective non-randomised comparative paediatric pilot study. A purposeful s le of children, requiring a urine microscopy for clinical management, presenting to one district emergency department was enrolled in the study to compare two non-invasive techniques of urine collection. Thirty-three patients were enrolled and satisfactory s les were obtained from 22 patients. The heavy (mixed growth) contamination rate in the UCP group (n=2 9.1%) versus the CCU group (n=1 4.5%) was not statistically significant (p=0.50 by Fisher's exact test). The rate of agreement (n=20 91%) in diagnosing or excluding urinary tract infection between the two groups was high. The median time to urine collection between the two groups (UCP method 30 min CCU 107.5 min) was statistically significant (p<0.002, Mann-Whitney U test). This study suggests that UCPs are practicable in Australasian Emergency Departments and may lead to faster diagnosis, disposition and reduced hospital stay.
Familial multiple lipomatosis with clear autosomal dominant inheritance and onset in early adolescence
Publisher: BMJ
Date: 17-02-2011
Novel de novo mutations of the interleukin-10 receptor gene lead to infantile onset inflammatory bowel disease
Publisher: Oxford University Press (OUP)
Date: 11-2014
DOI: 10.1016/J.CROHNS.2014.04.004
Abstract: Defects in the interleukin 10 (IL-10) signalling pathway have been shown to cause very early onset inflammatory bowel disease (IBD). We report a patient with severe infantile-onset IBD with a compound heterozygous IL-10 receptor alpha subunit (IL-10RA) mutation, one of which was paternally-inherited and the other occurring de novo. Deep sequencing of IL-10, IL-10RA and IL-10 receptor beta subunit (IL-10RB) were performed. Peripheral blood mononuclear cell (PBMC) surface expression of IL-10RA was analysed by flow cytometry. IL-10 signalling pathway was examined by measuring phosphorylated STAT3 in PBMC cultured in the presence of IL-6 or IL-10. We identified a missense mutation in exon 4 of IL-10RA (c.583T>C) in one allele and a nonsense mutation in exon 7 of IL-10RA (c.1368G>T) in the other allele. Neither mutation has been reported previously. The patient has functional IL-10RA deficiency despite normal IL-10RA expression. This represents the first case report of a de novo mutation of IL-10RA that is associated with very early onset severe IBD. Therefore, IL-10 pathway defect should be considered in patients with infantile-onset IBD even if the parents are non-consanguineous.
Rectal Juvenile Polyp Detected on 99mTc-Pertechnetate Abdominal Scintigraphy (Meckel Scan)
Publisher: Ovid Technologies (Wolters Kluwer Health)
Date: 06-2013
Prophylactic gastrectomy in a 16-year-old
Publisher: Ovid Technologies (Wolters Kluwer Health)
Date: 03-2014
Obstructive sleep apnoea in a paediatric patient with laryngeal Crohn's disease
Publisher: Wiley
Date: 06-11-2018
DOI: 10.1111/JPC.13753
Raised tacrolimus level and acute renal injury associated with acute gastroenteritis in a child receiving local rectal tacrolimus
Publisher: Springer Science and Business Media LLC
Date: 24-04-2014
DOI: 10.1007/S12328-014-0482-6
Abstract: Local rectal application of tacrolimus in distal colitis, pouchitis and perianal Crohn's disease has previously been reported to be both effective and safe. We report a patient treated with per rectum local application of tacrolimus, who developed toxic levels of tacrolimus and acute renal injury during an episode of acute gastroenteritis. This case illustrates that local application of tacrolimus, although usually safe, may be associated with significantly raised tacrolimus levels and acute renal injury during acute gastroenteritis. It is important for physicians to be aware of this association when prescribing local rectal tacrolimus.
IL-10 Potentiates Differentiation of Human Induced Regulatory T Cells via STAT3 and Foxo1
Publisher: The American Association of Immunologists
Date: 15-10-2015
Abstract: Foxp3+ regulatory T cells (Tregs) play essential roles in maintaining the immune balance. Although the majority of Tregs are formed in the thymus, increasing evidence suggests that induced Tregs (iTregs) may be generated in the periphery from naive cells. However, unlike in the murine system, significant controversy exists regarding the suppressive capacity of these iTregs in humans, especially those generated in vitro in the presence of TGF-β. Although it is well known that IL-10 is an important mediator of Treg suppression, the action of IL-10 on Tregs themselves is less well characterized. In this article, we show that the presence of IL-10, in addition to TGF-β, leads to increased expansion of Foxp3+ iTregs with enhanced CTLA-4 expression and suppressive capability, comparable to that of natural Tregs. This process is dependent on IL-10R–mediated STAT3 signaling, as supported by the lack of an IL-10 effect in patients with IL-10R deficiency and dominant-negative STAT3 mutation. Additionally, IL-10–induced inhibition of Akt phosphorylation and subsequent preservation of Foxo1 function are critical. These results highlight a previously unrecognized function of IL-10 in human iTreg generation, with potential therapeutic implications for the treatment of immune diseases, such as autoimmunity and allergy.
IL-2 Enhances Gut Homing Potential of Human Naive Regulatory T Cells Early in Life.
Publisher: The American Association of Immunologists
Date: 15-06-2018
Abstract: Recent evidence suggests early environmental factors are important for gut immune tolerance. Although the role of regulatory T (Treg) cells for gut immune homeostasis is well established, the development and tissue homing characteristics of Treg cells in children have not been studied in detail. In this article, we studied the development and homing characteristics of human peripheral blood Treg cell subsets and potential mechanisms inducing homing molecule expression in healthy children. We found contrasting patterns of circulating Treg cell gut and skin tropism, with abundant β7 integrin+ Treg cells at birth and increasing cutaneous lymphocyte Ag (CLA+) Treg cells later in life. β7 integrin+ Treg cells were predominantly naive, suggesting acquisition of Treg cell gut tropism early in development. In vitro, IL-7 enhanced gut homing but reduced skin homing molecule expression in conventional T cells, whereas IL-2 induced a similar effect only in Treg cells. This effect was more pronounced in cord compared with adult blood. Our results suggest that early in life, naive Treg cells may be driven for gut tropism by their increased sensitivity to IL-2–induced β7 integrin upregulation, implicating a potential role of IL-2 in gut immune tolerance during this critical period of development.
Low-dose IL-2 may enhance the effect of vedolizumab
Publisher: Informa UK Limited
Date: 14-06-2022
Split-graft liver transplantation from an adult donor with an unrecognized UCD to a pediatric and adult recipient
Publisher: Wiley
Date: 16-10-2018
DOI: 10.1111/PETR.13073
Abstract: We report the outcomes of an adult and pediatric split liver transplant from an adult male donor who died due to an unrecognized UCD, OTC deficiency. Recognizing inborn errors of metabolism can be challenging, especially in adult centers where such disorders are rarely encountered. Shortage of donors for liver transplantation has led to procedures to maximize donor utilization, such as split and live donor grafts. The cause of death should be ascertained before accepting a cadaveric donor organ.
Related Organisations
Queen's University Belfast
Location: United Kingdom of Great Britain and Northern Ireland
Related Funding Activities
No related grants have been discovered for Cheng Hiang Lee.