ORCID Profile
0000-0001-9782-6666
Current Organisations
Eijkman Institute for Molecular Biology
,
Garvan Institute of Medical Research
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Publisher: Public Library of Science (PLoS)
Date: 18-02-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2012
DOI: 10.1039/C2RA20232H
Publisher: Public Library of Science (PLoS)
Date: 14-07-2020
Publisher: Oxford University Press (OUP)
Date: 07-2017
Publisher: American Society of Tropical Medicine and Hygiene
Date: 04-2020
Publisher: Wiley
Date: 11-08-2011
DOI: 10.1002/JMV.22172
Abstract: The molecular epidemiological study of hepatitis B virus (HBV) in Indonesia is still limited. This study was aimed to identify the prevalence of HBV pre-S deletion/insertion mutations, and to assess the association of pre-S deletion mutation with liver disease progression in Indonesia. Pre-S mutations were identified by direct sequencing. Of the 265 subjects, 32 s les (12.1%) harbored pre-S deletion/insertion mutations. The prevalence of those pre-S mutations was 2.7% (2/75), 12.9% (8/62), 16.7% (11/66), and 17.7% (11/62) in asymptomatic carrier, chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma groups, respectively. Statistical analysis showed significant difference among them (P = 0.024). In HBV genotype B (HBV/B), pre-S1, pre-S1/S2, and pre-S2 deletion mutations were detected respectively in 3 (17.6%), 4 (23.5%), and 9 (52.9%) of 17 s les. On the other hand, in HBV/C, 12 of 15 s les (80.0%) showed a pre-S2 deletion mutation, and only 2 s les (13.3%) demonstrated a pre-S1/S2 deletion mutation. These results suggest that in HBV/B deletion mutation tends to occur in pre-S1 or pre-S1/S2 region, while in HBV/C the deletion mutation usually occurs in the pre-S2 region. Analysis of complete genome of four viruses confirmed that 3 isolates were classified into HBV/B3, and 1 isolate was HBV/C1. However, SimPlot and BootScan analyses showed that isolate 08.10.002 was an intragenotypic recombinant between HBV/B3 and HBV/B4. As conclusion, the prevalence of HBV pre-S mutations was relatively low in Indonesian patients compared to those from Taiwan, Japan, and other Asian countries. There was a weak association between pre-S deletion mutation and progressive liver disease.
Publisher: Elsevier BV
Date: 09-2019
DOI: 10.1016/J.IJID.2019.06.009
Abstract: Little is known about the birth prevalence and characteristics of congenital cytomegalovirus (CMV) infection in developing countries. To determine the prevalence and characteristics of congenital CMV infection in Indonesia, we conducted a prospective study in an urban birth cohort of neonates at a national referral hospital in 2016-2017, Jakarta, Indonesia. Consecutively born neonates were screened for the presence of CMV by using pan-herpesvirus nested-PCR and Sanger sequencing in saliva and/or urine specimens. Both the neonatal clinical findings as well as maternal characteristics were also evaluated. From a total of 411 newborns screened, congenital CMV infection was confirmed in 5.8% of the neonates. These CMV-positive newborns were more likely to have ventriculomegaly and thrombocytopenia compared to CMV-negative neonates. Notably, 67% CMV-positive neonates in our study had clinical findings that required medical intervention, from which only nine presented with symptoms suggestive of congenital CMV infection. Furthermore, congenital CMV infected babies were almost four times more likely to be born to mothers that had placenta previa and placental abruption. Our work highlights the high prevalence of congenital CMV infection in neonates born in one of the biggest referral hospitals in metropolitan Jakarta, Indonesia.
Publisher: Wiley
Date: 30-05-2020
DOI: 10.1111/IMR.12862
Publisher: SPIE
Date: 21-12-2008
DOI: 10.1117/12.759211
Publisher: Public Library of Science (PLoS)
Date: 16-11-2018
Publisher: Centers for Disease Control and Prevention (CDC)
Date: 09-2018
Publisher: American Chemical Society (ACS)
Date: 07-2011
DOI: 10.1021/LA201760W
Publisher: Baishideng Publishing Group Inc.
Date: 2009
DOI: 10.3748/WJG.15.4028
Publisher: American Chemical Society (ACS)
Date: 23-06-2011
DOI: 10.1021/AM2003526
Publisher: Wiley
Date: 19-01-2012
Publisher: Springer Science and Business Media LLC
Date: 19-10-2023
Publisher: Elsevier BV
Date: 12-2011
Publisher: Springer Science and Business Media LLC
Date: 19-08-2009
Publisher: Wiley
Date: 11-09-2009
DOI: 10.1111/J.1478-3231.2010.02280.X
Abstract: Hepatitis C virus (HCV) genotype distribution in Indonesia has been reported. However, the identification of HCV genotype was based on 5'-UTR or NS5B sequence. This study was aimed to observe HCV core sequence variation among HCV-associated liver disease patients in Jakarta, and to analyse the HCV genotype ersity based on the core sequence. Sixty-eight chronic hepatitis (CH), 48 liver cirrhosis (LC) and 34 hepatocellular carcinoma (HCC) were included in this study. HCV core variation was analysed by direct sequencing. Alignment of HCV core sequences demonstrated that the core sequence was relatively varied among the genotype. Indeed, 237 bases of the core sequence could classify the HCV subtype however, 236 bases failed to differentiate several subtypes. Based on 237 bases of the core sequences, the HCV strains were classified into genotypes 1 (subtypes 1a, 1b and 1c), 2 (subtypes 2a, 2e and 2f) and 3 (subtypes 3a and 3k). The HCV 1b (47.3%) was the most prevalent, followed by subtypes 1c (18.7%), 3k (10.7%), 2a (10.0%), 1a (6.7%), 2e (5.3%), 2f (0.7%) and 3a (0.7%). HCV 1b was the most common in all patients, and the prevalence increased with the severity of liver disease (36.8% in CH, 54.2% in LC and 58.8% in HCC). These results were similar to a previous report based on NS5B sequence analysis. Hepatitis C virus core sequence (237 bases) could identify the HCV subtype and the prevalence of HCV subtype based on core sequence was similar to those based on the NS5B region.
Publisher: Baishideng Publishing Group Inc.
Date: 2012
Location: United States of America
No related grants have been discovered for Rama Dhenni.