ORCID Profile
0000-0003-3999-0489
Current Organisations
Universidade de São Paulo
,
Hospital Israelita Albert Einstein
,
Universidade de São Paulo Faculdade de Medicina Hospital das Clínicas
,
INSPER
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Publications
Boosting Virology in Brazil
Publisher: Public Library of Science (PLoS)
Date: 11-03-2008
High frequency of lamivudine resistance mutations in Brazilian patients co-infected with HIV and hepatitis B
Publisher: Wiley
Date: 15-07-2010
DOI: 10.1002/JMV.21845
Abstract: This study analyzed the genotype distribution and frequency of lamivudine (LAM) and tenofovir (TDF) resistance mutations in a group of patients co-infected with HIV and hepatitis B virus (HBV). A cross-sectional study of 847 patients with HIV was conducted. Patients provided blood s les for HBsAg detection. The load of HBV was determined using an "in-house" real-time polymerase chain reaction. HBV genotypes/subgenotypes, antiviral resistance, basal core promoter (BCP), and precore mutations were detected by DNA sequencing. Twenty-eight patients with co-infection were identified. The distribution of HBV genotypes among these patients was A (n = 9 50%), D (n = 4 22.2%), G (n = 3 16.7%), and F (n = 2 11.1%). Eighteen patients were treated with LAM and six patients were treated with LAM plus TDF. The length of exposure to LAM and TDF varied from 4 to 216 months. LAM resistance substitutions (rtL180M + rtM204V) were detected in 10 (50%) of the 20 patients with viremia. This pattern and an accompanying rtV173L mutation was found in four patients. Three patients with the triple polymerase substitution pattern (rtV173L + rtL180M + rtM204V) had associated changes in the envelope gene (sE164D + sI195M). Mutations in the BCP region (A1762T, G1764A) and in the precore region (G1896A, G1899A) were also found. No putative TDF resistance substitution was detected. The data suggest that prolonged LAM use is associated with the emergence of particular changes in the HBV genome, including substitutions that may elicit a vaccine escape phenotype. No putative TDF resistance change was detected after prolonged use of TDF.
Phylogenetic analysis of hepatitis B virus genotype F complete genome sequences from Chilean patients with chronic infection
Publisher: Wiley
Date: 07-07-2011
DOI: 10.1002/JMV.22129
Abstract: Molecular epidemiological data concerning the hepatitis B virus (HBV) in Chile are not known completely. Since the HBV genotype F is the most prevalent in the country, the goal of this study was to obtain full HBV genome sequences from patients infected chronically in order to determine their subgenotypes and the occurrence of resistance-associated mutations. Twenty-one serum s les from antiviral drug-naive patients with chronic hepatitis B were subjected to full-length PCR lification, and both strands of the whole genomes were fully sequenced. Phylogenetic analyses were performed along with reference sequences available from GenBank (n = 290). The sequences were aligned using Clustal X and edited in the SE-AL software. Bayesian phylogenetic analyses were conducted by Markov Chain Monte Carlo simulations (MCMC) for 10 million generations in order to obtain the substitution tree using BEAST. The sequences were also analyzed for the presence of primary drug resistance mutations using CodonCode Aligner Software. The phylogenetic analyses indicated that all sequences were found to be the HBV subgenotype F1b, clustered into four different groups, suggesting that erse lineages of this subgenotype may be circulating within this population of Chilean patients.
Social Networks Shape the Transmission Dynamics of Hepatitis C Virus
Publisher: Public Library of Science (PLoS)
Date: 23-06-2010
The reporting of theoretical health risks by the media: Canadian newspaper reporting of potential blood transmission of Creutzfeldt-Jakob disease
Publisher: Springer Science and Business Media LLC
Date: 05-01-2004
Related Organisations
Universidade De São Paulo Faculdade De Medicina Hospital Das Clínicas
Location: Brazil
Related Funding Activities
No related grants have been discovered for Joao Renato Rebello Pinho.