ORCID Profile
0000-0001-6825-9324
Current Organisations
University of Oxford
,
Royal College of Physicians
,
Oxford University Hospitals NHS Trust
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: Springer Science and Business Media LLC
Date: 08-05-2019
DOI: 10.1038/S41598-019-43524-9
Abstract: Advancing interventions to tackle the huge global burden of hepatitis B virus (HBV) infection depends on improved insights into virus epidemiology, transmission, within-host ersity, drug resistance and pathogenesis, all of which can be advanced through the large-scale generation of full-length virus genome data. Here we describe advances to a protocol that exploits the circular HBV genome structure, using isothermal rolling-circle lification to enrich HBV DNA, generating concatemeric licons containing multiple successive copies of the same genome. We show that this product is suitable for Nanopore sequencing as single reads, as well as for generating short-read Illumina sequences. Nanopore reads can be used to implement a straightforward method for error correction that reduces the per-read error rate, by comparing multiple genome copies combined into a single concatemer and by analysing reads generated from plus and minus strands. With this approach, we can achieve an improved consensus sequencing accuracy of 99.7% and resolve intra-s le sequence variants to form whole-genome haplotypes. Thus while Illumina sequencing may still be the most accurate way to capture within-s le ersity, Nanopore data can contribute to an understanding of linkage between polymorphisms within in idual virions. The combination of isothermal lification and Nanopore sequencing also offers appealing potential to develop point-of-care tests for HBV, and for other viruses.
Publisher: Elsevier BV
Date: 12-2020
Publisher: Elsevier BV
Date: 2019
Publisher: Elsevier BV
Date: 2010
DOI: 10.1016/J.CUB.2009.10.079
Abstract: Accurate limb placement helps animals and robots to walk on substrates that are uneven or contain gaps. Visual information is important in controlling limb placement in walking mammals but has received little attention in insects. We investigated whether desert locusts walking along a horizontal ladder use vision to control limb placement. High-speed video analysis showed that locusts targeted their front legs to specific rungs in the absence of any previous contact, suggesting that visual information alone is sufficient for targeting single steps. Comparison between the proportions of missed steps before and after monocular occlusion showed that monocular visual information was used to place the ipsilateral but not the contralateral front leg. Accurate placement also depended upon mechanosensory inputs from the antennae and proprioceptive feedback from the ipsilateral but not the contralateral forelimb. Locusts also compensated for the loss of inputs to one eye by altering their stepping pattern. Changing the rung position after initiation of a step showed that targeting of the front leg depends on visual information acquired before but not during a step. The trajectory was only modified after missing the rung. Our data show that locusts walking in environments where footholds are limited use visual and mechanosensory information to place their front legs.
Publisher: Public Library of Science (PLoS)
Date: 25-01-2019
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Sheila Lumley.