ORCID Profile
0000-0003-0101-1097
Current Organisation
University of Adelaide
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Publisher: Cold Spring Harbor Laboratory
Date: 26-05-2021
DOI: 10.1101/2021.05.26.445725
Abstract: Soybean plants are salinity (NaCl) sensitive, with their yield significantly decreased under moderately saline conditions. GmSALT3 is the dominant gene underlying a major QTL for salt tolerance in soybean. GmSALT3 encodes a transmembrane protein belonging to the plant cation roton exchanger (CHX) family. It is currently unknown through which molecular mechanism(s) the ER-localised GmSALT3 contributes to salinity tolerance, as its localisation excludes direct involvement in ion exclusion. In order to gain insights into potential molecular mechanism(s), we used RNA-seq analysis of roots from two soybean NILs (Near Isogenic Lines) NIL-S (salt-sensitive, Gmsalt3 ) and NIL-T (salt-tolerant, GmSALT3 ), grown under control and saline conditions (200 mM NaCl) at three time points (0h, 6h, and 3 days). Gene ontology (GO) analysis showed that NIL-T has greater responses aligned to oxidation reduction. ROS were shown less abundant and scavenging enzyme activity was higher in NIL-T, consistent with the RNA-seq data. Further analysis indicated that genes related to calcium signalling, vesicle trafficking and Casparian strip (CS) development were upregulated in NIL-T following salt treatment. We propose that GmSALT3 improves the ability of NIL-T to cope with saline stress through preventing ROS overaccumulation in roots, and potentially modulating Ca 2+ signalling, vesicle trafficking and formation of diffusion barriers. RNA-seq analysis revealed that GmSALT3, which confers improved salt tolerance on soybean, improves reactive oxygen species detoxification in roots.
Publisher: Wiley
Date: 08-12-2020
DOI: 10.1111/PCE.13947
Publisher: Wiley
Date: 05-2022
DOI: 10.1111/PPL.13709
Abstract: Soybean ( Glycine max ) is an important crop globally for food and edible oil production. Soybean plants are sensitive to salinity (NaCl), with significant yield decreases reported under saline conditions. GmSALT3 is the dominant gene underlying a major QTL for salt tolerance in soybean. GmSALT3 encodes a transmembrane protein belonging to the plant cation roton exchanger (CHX) family, and is predominately expressed in root phloem and xylem associated cells under both saline and non‐saline conditions. It is currently unknown through which molecular mechanism(s) the ER‐localised GmSALT3 contributes to salinity tolerance, as its localisation excludes direct involvement in ion exclusion. In order to gain insights into potential molecular mechanism(s), we used RNA‐seq analysis of roots from two soybean NILs (near isogenic lines) NIL‐S (salt‐sensitive, Gmsalt3 ), and NIL‐T (salt‐tolerant, GmSALT3 ), grown under control and saline conditions (200 mM NaCl) at three time points (0 h, 6 h, and 3 days). Gene ontology (GO) analysis showed that NIL‐T has greater responses aligned to oxidation reduction. ROS were less abundant and scavenging enzyme activity was greater in NIL‐T, consistent with the RNA‐seq data. Further analysis indicated that genes related to calcium signalling, vesicle trafficking and Casparian strip (CS) development were upregulated in NIL‐T following salt treatment. We propose that GmSALT3 improves the ability of NIL‐T to cope with saline stress through preventing ROS overaccumulation in roots, and potentially modulating Ca 2+ signalling, vesicle trafficking and formation of diffusion barriers.
Publisher: Frontiers Media SA
Date: 30-09-2016
Publisher: eLife Sciences Publications, Ltd
Date: 06-01-2022
DOI: 10.7554/ELIFE.70701
Abstract: Plant cells maintain a low luminal pH in the trans-Golgi-network/early endosome (TGN/EE), the organelle in which the secretory and endocytic pathways intersect. Impaired TGN/EE pH regulation translates into severe plant growth defects. The identity of the proton pump and proton/ion antiporters that regulate TGN/EE pH have been determined, but an essential component required to complete the TGN/EE membrane transport circuit remains unidentified − a pathway for cation and anion efflux. Here, we have used complementation, genetically encoded fluorescent sensors, and pharmacological treatments to demonstrate that Arabidopsis cation chloride cotransporter (CCC1) is this missing component necessary for regulating TGN/EE pH and function. Loss of CCC1 function leads to alterations in TGN/EE-mediated processes including endocytic trafficking, exocytosis, and response to abiotic stress, consistent with the multitude of phenotypic defects observed in ccc1 knockout plants. This discovery places CCC1 as a central component of plant cellular function.
Publisher: Springer Science and Business Media LLC
Date: 22-05-2020
DOI: 10.1038/S42003-020-0990-5
Abstract: During plant growth, sodium (Na + ) in the soil is transported via the xylem from the root to the shoot. While excess Na + is toxic to most plants, non-toxic concentrations have been shown to improve crop yields under certain conditions, such as when soil K + is low. We quantified grain Na + across a barley genome-wide association study panel grown under non-saline conditions and identified variants of a Class 1 HIGH-AFFINITY-POTASSIUM-TRANSPORTER ( HvHKT1 )-encoding gene responsible for Na + content variation under these conditions. A leucine to proline substitution at position 189 (L189P) in HvHKT1 disturbs its characteristic plasma membrane localisation and disrupts Na + transport. Under low and moderate soil Na + , genotypes containing HvHKT1:5 P189 accumulate high concentrations of Na + but exhibit no evidence of toxicity. As the frequency of HvHKT1:5 P189 increases significantly in cultivated European germplasm, we cautiously speculate that this non-functional variant may enhance yield potential in non-saline environments, possibly by offsetting limitations of low available K + .
Publisher: Wiley
Date: 04-11-2014
DOI: 10.1111/TPJ.12695
Abstract: The identification of genes that improve the salt tolerance of crops is essential for the effective utilization of saline soils for agriculture. Here, we use fine mapping in a soybean (Glycine max (L.) Merr.) population derived from the commercial cultivars Tiefeng 8 and 85-140 to identify GmSALT3 (salt tolerance-associated gene on chromosome 3), a dominant gene associated with limiting the accumulation of sodium ions (Na+) in shoots and a substantial enhancement in salt tolerance in soybean. GmSALT3 encodes a protein from the cation/H+ exchanger family that we localized to the endoplasmic reticulum and which is preferentially expressed in the salt-tolerant parent Tiefeng 8 within root cells associated with phloem and xylem. We identified in the salt-sensitive parent, 85-140, a 3.78-kb copia retrotransposon insertion in exon 3 of Gmsalt3 that truncates the transcript. By sequencing 31 soybean landraces and 22 wild soybean (Glycine soja) a total of nine haplotypes including two salt-tolerant haplotypes and seven salt-sensitive haplotypes were identified. By analysing the distribution of haplotypes among 172 Chinese soybean landraces and 57 wild soybean we found that haplotype 1 (H1, found in Tiefeng 8) was strongly associated with salt tolerance and is likely to be the ancestral allele. Alleles H2-H6, H8 and H9, which do not confer salinity tolerance, were acquired more recently. H1, unlike other alleles, has a wide geographical range including saline areas, which indicates it is maintained when required but its potent stress tolerance can be lost during natural selection and domestication. GmSALT3 is a gene associated with salt tolerance with great potential for soybean improvement.
Publisher: eLife Sciences Publications, Ltd
Date: 10-2021
Publisher: Cold Spring Harbor Laboratory
Date: 02-01-2020
DOI: 10.1101/2020.01.02.893073
Abstract: Plant cells maintain a low luminal pH in the Trans-Golgi-Network/Early Endosome (TGN/EE), the organelle in which the secretory and endocytic pathways intersect. Impaired TGN/EE pH regulation translates into severe plant growth defects. The identity of the proton pump and proton/ion antiporters that regulate TGN/EE pH have been determined, but an essential component required to complete the TGN/EE membrane transport circuit remains unidentified − a pathway for cation and anion efflux. Here, we have used complementation, genetically encoded fluorescent sensors, and pharmacological treatments to demonstrate that the TGN/EE localised Arabidopsis Cation Chloride Cotransporter (CCC1) is this missing component necessary for regulating TGN/EE pH and function. Loss of CCC1 function leads to alterations in TGN/EE-mediated processes including endo- and exocytosis, and trafficking to the vacuole, and response to abiotic stress, consistent with the multitude of phenotypes observed in ccc1 knockout plants. This discovery places CCC1 as a central component of plant cellular function.
No related grants have been discovered for Yue Qu.