ORCID Profile
0000-0002-2683-0817
Current Organisation
CEA - Centre National de Recherche en Genomique Humaine
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Publisher: Public Library of Science (PLoS)
Date: 23-11-2004
Publisher: Springer Science and Business Media LLC
Date: 26-07-2018
DOI: 10.1038/S41467-018-05325-Y
Abstract: Genetic, epigenetic, and environmental factors contribute to the multifactorial disorder progressive supranuclear palsy (PSP). Here, we study epigenetic changes by genome-wide analysis of DNA from postmortem tissue of forebrains of patients and controls and detect significant ( P 0.05) methylation differences at 717 CpG sites in PSP vs. controls. Four-hundred fifty-one of these sites are associated with protein-coding genes. While differential methylation only affects a few sites in most genes, DLX1 is hypermethylated at multiple sites. Expression of an antisense transcript of DLX1 , DLX1AS , is reduced in PSP brains. The amount of DLX1 protein is increased in gray matter of PSP forebrains. Pathway analysis suggests that DLX1 influences MAPT -encoded Tau protein. In a cell system, overexpression of DLX1 results in downregulation of MAPT while overexpression of DLX1AS causes upregulation of MAPT . Our observations suggest that altered DLX1 methylation and expression contribute to pathogenesis of PSP by influencing MAPT .
Publisher: American Association for the Advancement of Science (AAAS)
Date: 22-08-2014
Abstract: Many domesticated plants arose through the meeting of multiple genomes through hybridization and genome doubling, known as polyploidy. Chalhoub et al. sequenced the polyploid genome of Brassica napus , which originated from a recent combination of two distinct genomes approximately 7500 years ago and gave rise to the crops of rape oilseed (canola), kale, and rutabaga. B. napus has undergone multiple events affecting differently sized genetic regions where a gene from one progenitor species has been converted to the copy from a second progenitor species. Some of these gene conversion events appear to have been selected by humans as part of the process of domestication and crop improvement. Science , this issue p. 950
Publisher: Public Library of Science (PLoS)
Date: 12-11-2012
Publisher: Informa UK Limited
Date: 04-10-2021
Publisher: Springer Science and Business Media LLC
Date: 27-06-2016
DOI: 10.1038/NBT.3605
Abstract: DNA methylation patterns are altered in numerous diseases and often correlate with clinically relevant information such as disease subtypes, prognosis and drug response. With suitable assays and after validation in large cohorts, such associations can be exploited for clinical diagnostics and personalized treatment decisions. Here we describe the results of a community-wide benchmarking study comparing the performance of all widely used methods for DNA methylation analysis that are compatible with routine clinical use. We shipped 32 reference s les to 18 laboratories in seven different countries. Researchers in those laboratories collectively contributed 21 locus-specific assays for an average of 27 predefined genomic regions, as well as six global assays. We evaluated assay sensitivity on low-input s les and assessed the assays' ability to discriminate between cell types. Good agreement was observed across all tested methods, with licon bisulfite sequencing and bisulfite pyrosequencing showing the best all-round performance. Our technology comparison can inform the selection, optimization and use of DNA methylation assays in large-scale validation studies, biomarker development and clinical diagnostics.
Publisher: American Association for the Advancement of Science (AAAS)
Date: 12-09-2014
Location: France
No related grants have been discovered for Jorg Tost.