ORCID Profile
0000-0001-9825-273X
Current Organisation
Inari Agriculture (United States)
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In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Gene Expression (incl. Microarray and other genome-wide approaches) | Plant Physiology | Genetics | Quantitative Genetics (incl. Disease and Trait Mapping Genetics)
Climate Change Adaptation Measures | Expanding Knowledge in the Biological Sciences | Wheat |
Publisher: Springer Science and Business Media LLC
Date: 05-05-2009
Publisher: Springer Science and Business Media LLC
Date: 04-10-2019
DOI: 10.1007/S00122-019-03454-6
Abstract: Genetic control of grain yield and phenology was examined in the Excalibur/Kukri doubled haploid mapping population grown in 32 field experiments across the climatic zones of southern Australia, India and north-western Mexico where the wheat crop experiences drought and heat stress. A total of 128 QTL were identified for four traits: grain yield, thousand grain weight (TGW), days to heading and grain filling duration. These QTL included 24 QTL for yield and 27 for TGW, showing significant interactions with the environment ( Q * E ). We also identified 14 QTL with a significant, small main effects on yield across environments. The study focussed on a region of chromosome 1B where two main effect QTL were found for yield and TGW without the confounding effect of phenology. Excalibur was the source of favourable alleles: QYld.aww - 1B.2 with a peak at 149.5–150.1 cM and QTgw.aww - 1B at 168.5–171.4 cM. We developed near isogenic lines (NIL) for the interval including QYld.aww - 1B.2 and QTgw.aww - 1B and evaluated them under semi-controlled conditions. Significant differences in four pairs of NIL were observed for grain yield but not for TGW, confirming a positive effect of the Excalibur allele for QYld.aww - 1B.2 . The interval containing QYld.aww - 1B.2 was narrowed down to 2.9 cM which corresponded to a 2.2 Mbp genomic region on the chromosome 1B genomic reference sequence of cv. Chinese Spring and contained 39 predicted genes.
Publisher: Springer Science and Business Media LLC
Date: 07-10-2022
DOI: 10.1007/S00122-022-04230-9
Abstract: Utilising a nested association mapping (NAM) population-based GWAS, 98 stable marker-trait associations with 127 alleles unique to the exotic parents were detected for grain yield and related traits in wheat. Grain yield, thousand-grain weight, screenings and hectolitre weight are important wheat yield traits. An understanding of their genetic basis is crucial for improving grain yield in breeding programmes. Nested association mapping (NAM) populations are useful resources for the dissection of the genetic basis of complex traits such as grain yield and related traits in wheat. Coupled with phenotypic data collected from multiple environments, NAM populations have the power to detect quantitative trait loci and their multiple alleles, providing germplasm that can be incorporated into breeding programmes. In this study, we evaluated a large-scale wheat NAM population with two recurrent parents in unbalanced trials in nine erse Australian field environments over three years. By applying a single-stage factor analytical linear mixed model (FALMM) to the NAM multi-environment trials (MET) data and conducting a genome-wide association study (GWAS), we detected 98 stable marker-trait associations (MTAs) with their multiple alleles. 74 MTAs had 127 alleles that were derived from the exotic parents and were absent in either of the two recurrent parents. The exotic alleles had favourable effects on 46 MTAs of the 74 MTAs, for grain yield, thousand-grain weight, screenings and hectolitre weight. Two NAM RILs with consistently high yield in multiple environments were also identified, highlighting the potential of the NAM population in supporting plant breeding through provision of germplasm that can be readily incorporated into breeding programmes. The identified beneficial exotic alleles introgressed into the NAM population provide potential target alleles for the genetic improvement of wheat and further studies aimed at pinpointing the underlying genes.
Publisher: Springer Science and Business Media LLC
Date: 29-05-2009
Publisher: Springer Science and Business Media LLC
Date: 18-11-2008
Publisher: FapUNIFESP (SciELO)
Date: 03-2005
Publisher: Public Library of Science (PLoS)
Date: 04-02-2019
Publisher: Springer New York
Date: 2014
DOI: 10.1007/978-1-4939-0446-4_2
Abstract: Once the sequence is known for a gene of interest, it is usually possible to design markers to detect polymorphisms within the gene. Such markers can be particularly useful in plant breeding, especially if they detect the causal polymorphism within the gene and are diagnostic of the phenotype. In this chapter, we (1) discuss how gene sequences are obtained and aligned and how polymorphic sites can be identified or predicted (2) explain the principles of PCR primer design and PCR lification and provide guidelines for their application in the design and testing of markers (3) discuss detection methods for presence/absence (dominant) polymorphisms, length polymorphisms and single nucleotide polymorphisms (SNPs) and (4) outline some of the factors that affect the utility of markers in plant breeding and explain how markers can be evaluated (validated) for use in plant breeding.
Publisher: Springer Science and Business Media LLC
Date: 18-01-2008
Publisher: Wiley
Date: 29-10-2008
DOI: 10.1111/J.1755-0998.2008.02213.X
Abstract: Acca sellowiana has commercial potential because of the quality and the unique flavor of its fruit. Conservation of natural populations and management of breeding programmes would benefit from the availability of molecular markers that could be used to characterize levels and distribution of genetic variability. Thus, 13 microsatellite markers were developed from an enriched genomic library of A. sellowiana. They were characterized using 40 s les. The expected and observed heterozygosities ranged from 0.513 to 0.913 and from 0.200 to 0.889, respectively. These are the first microsatellite loci characterized from A. sellowiana that will contribute to improve researches on the genetic conservation, characterization and breeding.
Publisher: Springer Science and Business Media LLC
Date: 26-09-2015
Publisher: Springer Science and Business Media LLC
Date: 02-12-2013
DOI: 10.1038/SREP03399
Publisher: Wiley
Date: 12-08-2009
DOI: 10.1111/J.1755-0998.2009.02674.X
Abstract: Eleven polymorphic microsatellite loci were described for the mangrove crab, Ucides cordatus, an important fishery resource on the Brazilian coast. The number of alleles observed at each locus varied between eight and 23. Observed and expected mean heterozygosities were 0.791 and 0.893 respectively. Amplification of all loci was highly successful, under the same polymerase chain reaction conditions. With the exception of P2D3, all loci adhered to the assumptions of the Hardy-Weinberg equilibrium and did not present deviations reflecting linkage disequilibrium. Given this, these markers will be extremely useful in future management programmes for U. cordatus stocks.
Publisher: Springer Science and Business Media LLC
Date: 28-11-2008
Publisher: Frontiers Media SA
Date: 31-01-2020
Publisher: Springer Science and Business Media LLC
Date: 02-2015
Publisher: Springer Science and Business Media LLC
Date: 08-04-2010
Publisher: Wiley
Date: 2009
DOI: 10.1111/J.1755-0998.2008.02308.X
Abstract: Stylosanthes capitata is an important tropical pasture legume. Knowledge of genetic ersity and structure of S. capitata populations is of great importance for the conservation and germplasm management of this species. Thus, eight microsatellite markers were developed from an S. capitata-enriched library. They were characterized in 20 accessions from the germplasm collection of the Empresa Brasileira de Pesquisa Agropecuária (Embrapa). The observed and expected heterozygosities ranged from 0.16 to 0.85 and from 0.40 to 0.85, respectively. These microsatellites are the first set of molecular markers from this species and will contribute towards studies of genetic ersity, conservation and breeding of S. capitata.
Publisher: Springer Science and Business Media LLC
Date: 05-11-2010
Publisher: Wiley
Date: 29-08-2008
DOI: 10.1111/J.1755-0998.2008.02175.X
Abstract: Epidendrum puniceoluteum is an endemic orchid of Atlantic Rainforest, restricted to few populations only due to the destruction and fragmentation of its native habitat. Here, we report on the development of 10 microsatellite markers isolated from this orchid species. Genetic variability was characterized in two distant populations from Brazil coast. The number of alleles observed for each locus ranged from two to 12 and with an average of 6.4 alleles per locus. These microsatellites should be valuable tools for studying both fine-scale genetic structure of scattered E. puniceoluteum population and patterns will be useful genetic markers for other closely related taxa.
Publisher: Oxford University Press (OUP)
Date: 31-10-2013
DOI: 10.1093/JXB/ERT333
Abstract: Global food security demands the development and delivery of new technologies to increase and secure cereal production on finite arable land without increasing water and fertilizer use. There are several options for boosting wheat yields, but most offer only small yield increases. Wheat is an inbred plant, and hybrids hold the potential to deliver a major lift in yield and will open a wide range of new breeding opportunities. A series of technological advances are needed as a base for hybrid wheat programmes. These start with major changes in floral development and architecture to separate the sexes and force outcrossing. Male sterility provides the best method to block self-fertilization, and modifying the flower structure will enhance pollen access. The recent explosion in genomic resources and technologies provides new opportunities to overcome these limitations. This review outlines the problems with existing hybrid wheat breeding systems and explores molecular-based technologies that could improve the hybrid production system to reduce hybrid seed production costs, a prerequisite for a commercial hybrid wheat system.
Publisher: MDPI AG
Date: 21-04-2021
DOI: 10.3390/IJMS22094348
Abstract: Genetic ersity, knowledge of the genetic architecture of the traits of interest and efficient means of transferring the desired genetic ersity into the relevant genetic background are prerequisites for plant breeding. Exotic germplasm is a rich source of genetic ersity however, they harbor undesirable traits that limit their suitability for modern agriculture. Nested association mapping (NAM) populations are valuable genetic resources that enable incorporation of genetic ersity, dissection of complex traits and providing germplasm to breeding programs. We developed the OzNAM by crossing and backcrossing 73 erse exotic parents to two Australian elite varieties Gladius and Scout. The NAM parents were genotyped using the iSelect wheat 90K Infinium SNP array, and the progeny were genotyped using a custom targeted genotyping-by-sequencing assay based on molecular inversion probes designed to target 12,179 SNPs chosen from the iSelect wheat 90K Infinium SNP array of the parents. In total, 3535 BC1F4:6 RILs from 125 families with 21 to 76 lines per family were genotyped and we found 4964 polymorphic and multi-allelic haplotype markers that spanned the whole genome. A subset of 530 lines from 28 families were evaluated in multi-environment trials over three years. To demonstrate the utility of the population in QTL mapping, we chose to map QTL for maturity and plant height using the RTM-GWAS approach and we identified novel and known QTL for maturity and plant height.
Publisher: Wiley
Date: 21-06-2019
DOI: 10.1111/TPJ.14350
Abstract: Nuclear male-sterile mutants with non-conditional, recessive and strictly monogenic inheritance are useful for both hybrid and conventional breeding systems, and have long been a research focus for many crops. In allohexaploid wheat, however, genic redundancy results in rarity of such mutants, with the ethyl methanesulfonate-induced mutant ms5 among the few reported to date. Here, we identify TaMs5 as a glycosylphosphatidylinositol-anchored lipid transfer protein required for normal pollen exine development, and by transgenic complementation demonstrate that TaMs5-A restores fertility to ms5. We show ms5 locates to a centromere-proximal interval and has a sterility inheritance pattern modulated by TaMs5-D but not TaMs5-B. We describe two allelic forms of TaMs5-D, one of which is non-functional and confers mono-factorial inheritance of sterility. The second form is functional but shows incomplete dominance. Consistent with reduced functionality, transcript abundance in developing anthers was found to be lower for TaMs5-D than TaMs5-A. At the 3B homoeolocus, we found only non-functional alleles among 178 erse hexaploid and tetraploid wheats that include landraces and Triticum dicoccoides. Apparent ubiquity of non-functional TaMs5-B alleles suggests loss-of-function arose early in wheat evolution and, therefore, at most knockout of two homoeoloci is required for sterility. This work provides genetic information, resources and tools required for successful implementation of ms5 sterility in breeding systems for bread and durum wheats.
Publisher: Oxford University Press (OUP)
Date: 2012
Publisher: Springer Science and Business Media LLC
Date: 24-03-2021
DOI: 10.1007/S00122-021-03792-4
Abstract: A correction to this paper has been published: 0.1007/s00122-021-03792-4
Publisher: Springer Science and Business Media LLC
Date: 12-2018
Publisher: Centro Internacional de Agricultura Tropical
Date: 2013
Publisher: Springer Science and Business Media LLC
Date: 04-10-2009
Publisher: FapUNIFESP (SciELO)
Date: 03-2005
Publisher: Springer Science and Business Media LLC
Date: 11-10-2017
DOI: 10.1038/S41467-017-00945-2
Abstract: The current rate of yield gain in crops is insufficient to meet the predicted demands. Capturing the yield boost from heterosis is one of the few technologies that offers rapid gain. Hybrids are widely used for cereals, maize and rice, but it has been a challenge to develop a viable hybrid system for bread wheat due to the wheat genome complexity, which is both large and hexaploid. Wheat is our most widely grown crop providing 20% of the calories for humans. Here, we describe the identification of Ms1 , a gene proposed for use in large-scale, low-cost production of male-sterile ( ms ) female lines necessary for hybrid wheat seed production. We show that Ms1 completely restores fertility to ms1d , and encodes a glycosylphosphatidylinositol-anchored lipid transfer protein, necessary for pollen exine development. This represents a key step towards developing a robust hybridization platform in wheat.
Start Date: 07-2016
End Date: 12-2020
Amount: $517,000.00
Funder: Australian Research Council
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