ORCID Profile
0000-0002-7232-5889
Current Organisations
SDU
,
University of Bonn
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Research Topics
In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Top 5 Research Topics
ANZSRC Field of Research (FoR)
Gene Expression (incl. Microarray and other genome-wide approaches) | Plant Cell and Molecular Biology | Plant Biology | Plant Physiology |
ANZSRC Socio-Economic Objective (SEO)
Related Links
Publications
GmSALT3 expression improves reactive oxygen species detoxification in salt-stressed soybean roots
Publisher: Cold Spring Harbor Laboratory
Date: 26-05-2021
DOI: 10.1101/2021.05.26.445725
Abstract: Soybean plants are salinity (NaCl) sensitive, with their yield significantly decreased under moderately saline conditions. GmSALT3 is the dominant gene underlying a major QTL for salt tolerance in soybean. GmSALT3 encodes a transmembrane protein belonging to the plant cation roton exchanger (CHX) family. It is currently unknown through which molecular mechanism(s) the ER-localised GmSALT3 contributes to salinity tolerance, as its localisation excludes direct involvement in ion exclusion. In order to gain insights into potential molecular mechanism(s), we used RNA-seq analysis of roots from two soybean NILs (Near Isogenic Lines) NIL-S (salt-sensitive, Gmsalt3 ) and NIL-T (salt-tolerant, GmSALT3 ), grown under control and saline conditions (200 mM NaCl) at three time points (0h, 6h, and 3 days). Gene ontology (GO) analysis showed that NIL-T has greater responses aligned to oxidation reduction. ROS were shown less abundant and scavenging enzyme activity was higher in NIL-T, consistent with the RNA-seq data. Further analysis indicated that genes related to calcium signalling, vesicle trafficking and Casparian strip (CS) development were upregulated in NIL-T following salt treatment. We propose that GmSALT3 improves the ability of NIL-T to cope with saline stress through preventing ROS overaccumulation in roots, and potentially modulating Ca 2+ signalling, vesicle trafficking and formation of diffusion barriers. RNA-seq analysis revealed that GmSALT3, which confers improved salt tolerance on soybean, improves reactive oxygen species detoxification in roots.
Soybean CHX‐type ion transport protein GmSALT3 confers leaf Na+ exclusion via a root derived mechanism, and Cl− exclusion via a shoot derived process
Publisher: Wiley
Date: 08-12-2020
DOI: 10.1111/PCE.13947
An investigation into modelling approaches for industrial symbiosis: A literature review and research agenda
Publisher: Elsevier BV
Date: 03-2022
A single nucleotide substitution in TaHKT1;5‐D controls shoot Na+ accumulation in bread wheat
Publisher: Wiley
Date: 22-07-2020
DOI: 10.1111/PCE.13841
Expression of the mammalian Xenotropic Polytropic Virus Receptor 1 (XPR1) in tobacco leaves leads to phosphate export
Publisher: Wiley
Date: 25-12-2013
DOI: 10.1016/J.FEBSLET.2013.12.013
Abstract: Phosphate homeostasis in multicellular eukaryotes depends on both phosphate influx and efflux. The mammalian Xenotropic Polytropic Virus Receptor 1 (XPR1) shares homology to the Arabidopsis PHO1, a phosphate exporter expressed in roots. However, phosphate export activity of XPR1 has not yet been demonstrated in a heterologous system. Here, wedemonstrate that transient expression in tobacco leaves of XPR1-GFP leads to specific phosphate export. Like PHO1-GFP, XPR1-GFP is localized predominantly to the endomembrane system in tobacco cells. These results show that tobacco leaves are a good heterologous system to study the transport activity of members of the PHO1/XPR1 family.
Highly Efficient Virus-induced Gene Silencing (VIGS) in California Poppy (Eschscholzia californica): An Evaluation of VIGS as a Strategy to Obtain Functional Data from Non-model Plants
Publisher: Oxford University Press (OUP)
Date: 06-07-2007
DOI: 10.1093/AOB/MCM118
Manganese management in plants: Golgi transporter determines manganese allocation and cell wall composition
Publisher: Oxford University Press (OUP)
Date: 19-09-2022
Author response: Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)
Publisher: eLife Sciences Publications, Ltd
Date: 10-2021
Functional expression of PHO1 to the Golgi and trans‐Golgi network and its role in export of inorganic phosphate
Publisher: Wiley
Date: 25-05-2012
Grapevine and Arabidopsis cation-chloride cotransporters localise to the Golgi and trans-Golgi network and indirectly influence long-distance ion homeostasis and plant salt tolerance
Publisher: Oxford University Press (OUP)
Date: 16-09-2015
DOI: 10.1104/PP.15.00499
The EXS Domain of PHO1 Participates in the Response of Shoots to Phosphate Deficiency via a Root-to-Shoot Signal
Publisher: Oxford University Press (OUP)
Date: 06-11-2015
DOI: 10.1104/PP.15.00975
A single residue deletion in the barley HKT1;5 P189 variant restores plasma membrane localisation but not Na+ conductance
Publisher: Elsevier BV
Date: 10-2021
Correction: Corrigendum: GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters
Publisher: Springer Science and Business Media LLC
Date: 28-08-2015
DOI: 10.1038/NCOMMS9293
Abstract: Nature Communications 6, Article number: 7879 (2015) Published: 29 July 2015 Updated: 28 August 2015. The original version of this Article contained a typographical error in the spelling of the author Matthew Gilliham, which was incorrectly given as Matthew Gillham. This has now been corrected in both the PDF and HTML versions of the Article.
The proline 160 in the selectivity filter of the Arabidopsis NO3−/H+ exchanger AtCLCa is essential for nitrate accumulation in planta
Publisher: Wiley
Date: 25-08-2010
The transition to electric vehicles and a net zero economy: A model based on circular economy, stakeholder theory, and system thinking approach
Publisher: Elsevier BV
Date: 07-2023
Sweet or sour? important link between nitrate signaling and malate accumulation identified in apple
Publisher: Oxford University Press (OUP)
Date: 06-2020
DOI: 10.1104/PP.20.00535
GmSALT3 confers shoot Na+ and Cl− exclusion in soybean via two distinct processes
Publisher: Cold Spring Harbor Laboratory
Date: 07-01-2020
DOI: 10.1101/2020.01.06.896456
Abstract: Soybean ( Glycine max ) yields are threatened by multiple stresses including soil salinity. GmSALT3 confers net shoot exclusion for both Na+ and Cl − and improves salt tolerance of soybean however, how the ER-localised GmSALT3 achieves this is unknown. Here, GmSALT3’s function was investigated in heterologous systems and near-isogenic lines that contained the full-length GmSALT3 (NIL-T salt-tolerant) or a truncated transcript Gmsalt3 (NIL-S salt-sensitive). GmSALT3 restored growth of K+-uptake-defective E. coli and contributed toward net influx and accumulation of Na+, K+, and Cl − in Xenopus laevis oocytes, while Gmsalt3 was non-functional. A time-course analysis of the NILs confirmed that shoot Cl − exclusion breaks down prior to Na+ exclusion, while grafting showed that shoot Na + exclusion occurs via a root xylem-based mechanism. In contrast, NIL-T plants exhibited significantly greater Cl − content in both the stem xylem and phloem sap compared to NIL-S, indicating that shoot Cl − exclusion likely depends upon novel phloem-based Cl − recirculation. NIL-T shoots grafted on NIL-S roots contained low shoot Cl − , which confirmed that Cl − recirculation is dependent on the presence of GmSALT3 in shoots. Overall, these findings provide new insights on GmSALT3’s impact on salinity tolerance and reveal a novel mechanism for shoot Cl– exclusion in plants. GmSALT3 improves soybean salt tolerance. Here, using heterologous expression, we found GmSALT3 is a functional ion transporter, and, in planta that it confers shoot salt exclusion through root-based Na + xylem exclusion and shoot-based Cl − exclusion via phloem derived Cl - recirculation.
Plant Uncoupling Mitochondrial Protein 2 localizes to the Golgi
Publisher: Oxford University Press (OUP)
Date: 11-10-2023
Novel Electrical Signaling: First Fast Voltage-Gated Sodium Channel Identified Outside of the Animal Kingdom
Publisher: Oxford University Press (OUP)
Date: 12-2020
DOI: 10.1104/PP.20.01400
Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5
Publisher: Springer Science and Business Media LLC
Date: 22-05-2020
DOI: 10.1038/S42003-020-0990-5
Abstract: During plant growth, sodium (Na + ) in the soil is transported via the xylem from the root to the shoot. While excess Na + is toxic to most plants, non-toxic concentrations have been shown to improve crop yields under certain conditions, such as when soil K + is low. We quantified grain Na + across a barley genome-wide association study panel grown under non-saline conditions and identified variants of a Class 1 HIGH-AFFINITY-POTASSIUM-TRANSPORTER ( HvHKT1 )-encoding gene responsible for Na + content variation under these conditions. A leucine to proline substitution at position 189 (L189P) in HvHKT1 disturbs its characteristic plasma membrane localisation and disrupts Na + transport. Under low and moderate soil Na + , genotypes containing HvHKT1:5 P189 accumulate high concentrations of Na + but exhibit no evidence of toxicity. As the frequency of HvHKT1:5 P189 increases significantly in cultivated European germplasm, we cautiously speculate that this non-functional variant may enhance yield potential in non-saline environments, possibly by offsetting limitations of low available K + .
PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 2 localizes to the Golgi
Publisher: Cold Spring Harbor Laboratory
Date: 08-05-2023
DOI: 10.1101/2023.05.06.539686
Abstract: Mitochondria act as cellular hubs of energy transformation and metabolite conversion in most eukaryotes. Plant mitochondrial electron transport chains are particularly flexible, featuring alternative components, such as ALTERNATIVE NAD(P)H DEHYDROGENASES and ALTERNATIVE OXIDASES (AOXs), that can bypass proton translocation steps. PLANT UNCOUPLING MITOCHONDRIAL PROTEINS (named PUMPs or plant UCPs) have been identified in plants as homologues of mammalian Uncoupling Proteins (UCPs), and their biochemical and physiological roles have been investigated in the context of mitochondrial energy metabolism. To dissect UCP function in Arabidopsis, the two most conserved (UCP1 and UCP2) have been targeted in recent work by combining mutant lines to circumvent potential functional redundancy in vivo . Such approaches rely on the assumption that both proteins reside in the inner mitochondrial membrane as a prerequisite for functional redundancy. Yet, contradicting results have been reported on UCP2 localization in plants. Here we provide evidence that, conversely to UCP1, which is an abundant inner mitochondrial membrane protein, UCP2 localizes to the Golgi rather than to mitochondria. Based on multiple lines of new and prior evidence, we summarize the consensus view that we have reached and provide an ex le of how open, critical exchange within the research community is able to constructively address ambiguities. Our observations and considerations provide direction to the ongoing discussion about the functions of UCP proteins. They further offer new perspectives for the study of Golgi membrane transport and subcellular targeting principles of membrane proteins. Since 20 to 30 % of genes in plant genomes are predicted to encode transmembrane proteins and the function of most of those proteins has not been experimentally investigated, we highlight the importance of using independent evidence for localization as a prerequisite for understanding physiological function of membrane proteins.
Energy costs of salt tolerance in crop plants
Publisher: Wiley
Date: 11-07-2019
DOI: 10.1111/NPH.15864
Abstract: Agriculture is expanding into regions that are affected by salinity. This review considers the energetic costs of salinity tolerance in crop plants and provides a framework for a quantitative assessment of costs. Different sources of energy, and modifications of root system architecture that would maximize water vs ion uptake are addressed. Energy requirements for transport of salt (NaCl) to leaf vacuoles for osmotic adjustment could be small if there are no substantial leaks back across plasma membrane and tonoplast in root and leaf. The coupling ratio of the H
Root cell wall solutions for crop plants in saline soils
Publisher: Elsevier BV
Date: 04-2018
DOI: 10.1016/J.PLANTSCI.2017.12.012
Abstract: The root growth of most crop plants is inhibited by soil salinity. Roots respond by modulating metabolism, gene expression and protein activity, which results in changes in cell wall composition, transport processes, cell size and shape, and root architecture. Here, we focus on the effects of salt stress on cell wall modifying enzymes, cellulose microfibril orientation and non-cellulosic polysaccharide deposition in root elongation zones, as important determinants of inhibition of root elongation, and highlight cell wall changes linked to tolerance to salt stressed and water limited roots. Salt stress induces changes in the wall composition of specific root cell types, including the increased deposition of lignin and suberin in endodermal and exodermal cells. These changes can benefit the plant by preventing water loss and altering ion transport pathways. We suggest that binding of Na
Plants Increase Photosynthesis Efficiency by Lowering the Proton Gradient across the Thylakoid Membrane
Publisher: Oxford University Press (OUP)
Date: 04-2020
DOI: 10.1104/PP.20.00273
Metal health: PATELLIN2 reduces iron-induced toxicity in Arabidopsis
Publisher: Oxford University Press (OUP)
Date: 15-02-2023
Anion Channels/Transporters in Plants: From Molecular Bases to Regulatory Networks
Publisher: Annual Reviews
Date: 02-06-2011
DOI: 10.1146/ANNUREV-ARPLANT-042110-103741
Abstract: Anion channels/transporters are key to a wide spectrum of physiological functions in plants, such as osmoregulation, cell signaling, plant nutrition and compartmentalization of metabolites, and metal tolerance. The recent identification of gene families encoding some of these transport systems opened the way for gene expression studies, structure-function analyses of the corresponding proteins, and functional genomics approaches toward further understanding of their integrated roles in planta. This review, based on a few selected ex les, illustrates that the members of a given gene family exhibit a ersity of substrate specificity, regulation, and intracellular localization, and are involved in a wide range of physiological functions. It also shows that post-translational modifications of transport proteins play a key role in the regulation of anion transport activity. Key questions arising from the increasing complexity of networks controlling anion transport in plant cells (the existence of redundancy, cross talk, and coordination between various pathways and compartments) are also addressed.
Enhanced reactive oxygen detoxification occurs in salt‐stressed soybean roots expressing GmSALT3
Publisher: Wiley
Date: 05-2022
DOI: 10.1111/PPL.13709
Abstract: Soybean ( Glycine max ) is an important crop globally for food and edible oil production. Soybean plants are sensitive to salinity (NaCl), with significant yield decreases reported under saline conditions. GmSALT3 is the dominant gene underlying a major QTL for salt tolerance in soybean. GmSALT3 encodes a transmembrane protein belonging to the plant cation roton exchanger (CHX) family, and is predominately expressed in root phloem and xylem associated cells under both saline and non‐saline conditions. It is currently unknown through which molecular mechanism(s) the ER‐localised GmSALT3 contributes to salinity tolerance, as its localisation excludes direct involvement in ion exclusion. In order to gain insights into potential molecular mechanism(s), we used RNA‐seq analysis of roots from two soybean NILs (near isogenic lines) NIL‐S (salt‐sensitive, Gmsalt3 ), and NIL‐T (salt‐tolerant, GmSALT3 ), grown under control and saline conditions (200 mM NaCl) at three time points (0 h, 6 h, and 3 days). Gene ontology (GO) analysis showed that NIL‐T has greater responses aligned to oxidation reduction. ROS were less abundant and scavenging enzyme activity was greater in NIL‐T, consistent with the RNA‐seq data. Further analysis indicated that genes related to calcium signalling, vesicle trafficking and Casparian strip (CS) development were upregulated in NIL‐T following salt treatment. We propose that GmSALT3 improves the ability of NIL‐T to cope with saline stress through preventing ROS overaccumulation in roots, and potentially modulating Ca 2+ signalling, vesicle trafficking and formation of diffusion barriers.
The grapevine NaE sodium exclusion locus encodes sodium transporters with diverse transport properties and localisation
Publisher: Elsevier BV
Date: 03-2020
Identification of a unique ZIP transporter involved in zinc uptake via the arbuscular mycorrhizal fungal pathway
Publisher: Cold Spring Harbor Laboratory
Date: 29-09-2020
DOI: 10.1101/2020.09.28.317669
Abstract: Low soil zinc (Zn) availability is a limiting factor for crop yield, and increasing Zn content is a major target for the biofortification of major crops. Arbuscular mycorrhizal (AM) fungi associate with the roots of most terrestrial plant species and improve the host plant’s growth and nutrition through the mycorrhizal pathway of nutrient uptake. Although the physiology of Zn uptake through the mycorrhizal pathway is well established, the identity of the molecular components responsible for Zn transport in the mycorrhizal pathway are unknown. RNA-seq analysis identified the putative Zn transporter gene MtZIP14 by its marked up-regulation in Medicago truncatula roots when colonised by the AM fungus Rhizophagus irregularis under varying soil Zn supply. Expression of GFP-tagged MtZIP14 in roots revealed that it is exclusively localised to the site of plant-fungal nutrient exchange in cortical cells, the peri-arbuscular membrane. Expression of MtZIP14 in a yeast mutant lacking Zn transport function restored growth under low Zn availability. M. truncatula MtZIP14 loss-of-function mutants had reduced shoot biomass compared to the wild-type when colonised by AM fungi and grown under low Zn. Vesicular and arbuscular colonisation, but not hyphal colonisation, were also lower in mtzip14 mutant plants. Based on these results we propose that MtZIP14 plays a key role in the transport of Zn from AM fungus to plant across the peri-arbuscular membrane, and MtZIP14 function is crucial to plant competitiveness in a low Zn soil. Majority of crop plant species associate with arbuscular mycorrhizal fungi, which can increase plant nutrient uptake. Improving our knowledge of how Zn is taken up in mycorrhizal plants will lead to improved plant and human Zn nutrition outcomes. Here, we report a novel plant transporter with a major role in Zn nutrition of mycorrhizal plants. MtZIP14 is involved in Zn transport, is exclusively localised to the specialised plant-fungal interface in roots, and impairment of MtZIP14 gene function results in negative impacts on both plant growth and Zn nutrition.
A Grain of Salt
Publisher: Cold Spring Harbor Laboratory
Date: 23-01-2020
DOI: 10.1101/2020.01.23.916742
Abstract: We quantified grain sodium (Na + ) content across a barley GWAS panel grown under optimal conditions. We identified a strong association with a region containing two low and one high Na + accumulating haplotypes of a Class 1 HIGH-AFFINITY POTASSIUM TRANSPORTER (HKT1 ) known to be involved in regulating plant Na + homeostasis. The haplotypes exhibited an average 1.8-fold difference in grain Na + content. We show that an L189P substitution disrupts Na + transport in the high Na + lines, disturbs the plasma membrane localisation typical of HKT1 and induces a conformational change in the protein predicted to compromise function. Under NaCl stress, lines containing P189 accumulate high levels of Na + , but show no significant difference in biomass. P189 increases in frequency from wild-species to elite cultivars leading us to speculate that the compromised haplotype is undergoing directional selection possibly due to the value of Na + as a functional nutrient in non-saline environments.
The function of the Medicago truncatula ZIP transporter MtZIP14 is linked to arbuscular mycorrhizal fungal colonization
Publisher: Wiley
Date: 29-01-2023
DOI: 10.1111/PCE.14545
Abstract: Soil micronutrient availability, including zinc (Zn), is a limiting factor for crop yield. Arbuscular mycorrhizal (AM) fungi can improve host plant growth and nutrition through the mycorrhizal pathway of nutrient uptake. Although the physiology of Zn uptake through the mycorrhizal pathway is well established, the identity of the related molecular components are unknown. Here, RNA‐seq analysis was used to identify genes differentially‐regulated by AM colonization and soil Zn concentration in roots of Medicago truncatula . The putative Zn transporter gene MtZIP14 was markedly up‐regulated in M. truncatula roots when colonized by Rhizophagus irregularis . MtZIP14 restored yeast growth under low Zn availability. Loss‐of‐function mutant plants ( mtzip14 ) had reduced shoot biomass compared to the wild‐type when colonized by AM fungi and grown under low and sufficient soil Zn concentration at high soil Zn concentration, there were no genotypic differences in shoot biomass. The vesicular and arbuscular colonization of roots was lower in the mtzip14 plants regardless of soil Zn concentration. We propose that MtZIP14 is linked to AM colonization in M. truncatula plants, with the possibility that MtZIP14 function with AM colonization is linked to plant Zn nutrition.
GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters
Publisher: Springer Science and Business Media LLC
Date: 29-07-2015
DOI: 10.1038/NCOMMS8879
Abstract: The non-protein amino acid, gamma-aminobutyric acid (GABA) rapidly accumulates in plant tissues in response to biotic and abiotic stress, and regulates plant growth. Until now it was not known whether GABA exerts its effects in plants through the regulation of carbon metabolism or via an unidentified signalling pathway. Here, we demonstrate that anion flux through plant aluminium-activated malate transporter (ALMT) proteins is activated by anions and negatively regulated by GABA. Site-directed mutagenesis of selected amino acids within ALMT proteins abolishes GABA efficacy but does not alter other transport properties. GABA modulation of ALMT activity results in altered root growth and altered root tolerance to alkaline pH, acid pH and aluminium ions. We propose that GABA exerts its multiple physiological effects in plants via ALMT, including the regulation of pollen tube and root growth, and that GABA can finally be considered a legitimate signalling molecule in both the plant and animal kingdoms.
The flow of water: Critical factors of root axial water transport determined.
Publisher: Oxford University Press (OUP)
Date: 02-08-2022
ATP Binding to the C Terminus of the Arabidopsis thaliana Nitrate/Proton Antiporter, AtCLCa, Regulates Nitrate Transport into Plant Vacuoles
Publisher: Elsevier BV
Date: 09-2009
Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)
Publisher: eLife Sciences Publications, Ltd
Date: 06-01-2022
DOI: 10.7554/ELIFE.70701
Abstract: Plant cells maintain a low luminal pH in the trans-Golgi-network/early endosome (TGN/EE), the organelle in which the secretory and endocytic pathways intersect. Impaired TGN/EE pH regulation translates into severe plant growth defects. The identity of the proton pump and proton/ion antiporters that regulate TGN/EE pH have been determined, but an essential component required to complete the TGN/EE membrane transport circuit remains unidentified − a pathway for cation and anion efflux. Here, we have used complementation, genetically encoded fluorescent sensors, and pharmacological treatments to demonstrate that Arabidopsis cation chloride cotransporter (CCC1) is this missing component necessary for regulating TGN/EE pH and function. Loss of CCC1 function leads to alterations in TGN/EE-mediated processes including endocytic trafficking, exocytosis, and response to abiotic stress, consistent with the multitude of phenotypic defects observed in ccc1 knockout plants. This discovery places CCC1 as a central component of plant cellular function.
Plant Cation-Chloride Cotransporters (CCC): Evolutionary Origins and Functional Insights
Publisher: MDPI AG
Date: 06-02-2018
DOI: 10.3390/IJMS19020492
Post-Transcriptional Regulation of Nutrient Transporters
Publisher: Oxford University Press (OUP)
Date: 09-2020
DOI: 10.1104/PP.20.01017
A single nucleotide substitution in TaHKT1;5-D controls shoot Na+ accumulation in bread wheat
Publisher: Cold Spring Harbor Laboratory
Date: 24-01-2020
DOI: 10.1101/2020.01.21.909887
Abstract: Improving salinity tolerance in the most widely cultivated cereal, bread wheat ( Triticum aestivum L.), is essential to increase grain yields on saline agricultural lands. A Portuguese landrace, Mocho de Espiga Branca accumulates up to 6 folds greater leaf and sheath sodium (Na + ) than two Australian cultivars, Gladius and Scout, under salt stress. Despite high leaf and sheath Na + concentrations, Mocho de Espiga Branca maintained similar salinity tolerance compared to Gladius and Scout. A naturally occurring single nucleotide substitution was identified in the gene encoding a major Na + transporter TaHKT1 -D in Mocho de Espiga Branca, which resulted in a L190P amino acid residue variation. This variant prevents Mocho de Espiga Branca from retrieving Na + from the root xylem leading to a high shoot Na + concentration. The identification of the tissue tolerant Mocho de Espiga Branca will accelerate the development of more elite salt tolerant bread wheat cultivars.
Phosphorylation of the vacuolar anion exchanger AtCLCa is required for the stomatal response to abscisic acid
Publisher: American Association for the Advancement of Science (AAAS)
Date: 08-07-2014
DOI: 10.1126/SCISIGNAL.2005140
Abstract: The Arabidopsis vacuolar anion roton exchanger AtCLCa contributes to both opening and closing of stomata.
The CRABS CLAW ortholog from California poppy (Eschscholzia californica, Papaveraceae), EcCRC, is involved in floral meristem termination, gynoecium differentiation and ovule init
Publisher: Wiley
Date: 05-2009
Roles of membrane transporters: connecting the dots from sequence to phenotype
Publisher: Oxford University Press (OUP)
Date: 06-2019
DOI: 10.1093/AOB/MCZ066
Abstract: Plant membrane transporters are involved in erse cellular processes underpinning plant physiology, such as nutrient acquisition, hormone movement, resource allocation, exclusion or sequestration of various solutes from cells and tissues, and environmental and developmental signalling. A comprehensive characterization of transporter function is therefore key to understanding and improving plant performance. In this review, we focus on the complexities involved in characterizing transporter function and the impact that this has on current genomic annotations. Specific ex les are provided that demonstrate why sequence homology alone cannot be relied upon to annotate and classify transporter function, and to show how even single amino acid residue variations can influence transporter activity and specificity. Misleading nomenclature of transporters is often a source of confusion in transporter characterization, especially for people new to or outside the field. Here, to aid researchers dealing with interpretation of large data sets that include transporter proteins, we provide ex les of transporters that have been assigned names that misrepresent their cellular functions. Finally, we discuss the challenges in connecting transporter function at the molecular level with physiological data, and propose a solution through the creation of new databases. Further fundamental in-depth research on specific transport (and other) proteins is still required without it, significant deficiencies in large-scale data sets and systems biology approaches will persist. Reliable characterization of transporter function requires integration of data at multiple levels, from amino acid residue sequence annotation to more in-depth biochemical, structural and physiological studies.
Energy costs of salinity tolerance in crop plants
Publisher: Wiley
Date: 29-11-2019
DOI: 10.1111/NPH.15555
Chloride: not simply a ‘cheap osmoticum’, but a beneficial plant macronutrient
Publisher: Oxford University Press (OUP)
Date: 30-03-2017
DOI: 10.1093/JXB/ERX050
Abstract: At macronutrient levels, chloride has positive effects on plant growth, which are distinct from its function in photosynthesis..
Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)
Publisher: Cold Spring Harbor Laboratory
Date: 02-01-2020
DOI: 10.1101/2020.01.02.893073
Abstract: Plant cells maintain a low luminal pH in the Trans-Golgi-Network/Early Endosome (TGN/EE), the organelle in which the secretory and endocytic pathways intersect. Impaired TGN/EE pH regulation translates into severe plant growth defects. The identity of the proton pump and proton/ion antiporters that regulate TGN/EE pH have been determined, but an essential component required to complete the TGN/EE membrane transport circuit remains unidentified − a pathway for cation and anion efflux. Here, we have used complementation, genetically encoded fluorescent sensors, and pharmacological treatments to demonstrate that the TGN/EE localised Arabidopsis Cation Chloride Cotransporter (CCC1) is this missing component necessary for regulating TGN/EE pH and function. Loss of CCC1 function leads to alterations in TGN/EE-mediated processes including endo- and exocytosis, and trafficking to the vacuole, and response to abiotic stress, consistent with the multitude of phenotypes observed in ccc1 knockout plants. This discovery places CCC1 as a central component of plant cellular function.
Related Organisations
Related Funding Activities
Controlling Chloride In Plants
Start Date: 2016
End Date: 2019
Funder: Australian Research Council
View Funded ActivityDiscovery Early Career Researcher Award - Grant ID: DE160100804
Start Date: 06-2016
End Date: 06-2019
Amount: $370,000.00
Funder: Australian Research Council
View Funded Activity