ORCID Profile
0000-0001-8218-2538
Current Organisation
Technical University Dresden
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Publisher: Elsevier BV
Date: 10-2014
DOI: 10.1016/J.JRI.2014.05.003
Abstract: Epigenetic mechanisms such as DNA methylation, histone modification, and micro RNA signaling regulate the activity of the genome. Virtually all aspects of immunity involve some level of epigenetic regulation, whether it be host defense or in mediating tolerance. These processes are critically important in mediating dynamic responses to the environment over the life course of the in idual, yet we are only just beginning to understand how dysregulation in these pathways may play a role in immune disease. Here, we give a brief chronological overview of epigenetic processes during immune development in health and disease.
Publisher: Springer Science and Business Media LLC
Date: 14-09-2020
Publisher: Wiley
Date: 02-06-2014
DOI: 10.1096/FJ.13-249029
Publisher: S. Karger AG
Date: 2015
DOI: 10.1159/000442158
Abstract: b i Introduction: /i /b Chronic inflammatory diseases including allergies and asthma are the result of complex interactions between genes and environmental factors. Epigenetic mechanisms comprise a set of biochemical reactions that regulate gene expression. In order to understand the cause-effect relationship between environmental exposures and disease development, methods capable of assessing epigenetic regulation (also) in large cohorts are needed. b i Methods: /i /b For this purpose, we developed and evaluated a miniaturized chromatin immunoprecipitation (ChIP) assay allowing for a cost-effective assessment of histone acetylation of candidate genes in a quantitative fashion. This method was then applied to assess H3 and H4 histone acetylation changes in cord blood (CB) s les from an established cohort of Australian children exposed in the fetal period to either very low or very high levels of maternal folate. b i Results: /i /b Our ChIP assay was validated for a minimum requirement of 1 × 10 sup /sup target cells (e.g. CD4+ T cells). Very high levels of maternal folate were significantly associated with increased H3/H4 acetylation at i GATA3 /i and/or i IL9 /i promoter regions in CD4+ T cells in CB. b i Conclusion: /i /b We developed a ChIP method allowing reliable assessment of H3/H4 acetylation using 1 × 10 sup /sup cells only. Practical application of this assay demonstrated an association between high maternal folate exposure and increased histone acetylation, corresponding to a more transcriptionally permissive chromatin status in the promoter regions of some Th2-related genes.
No related grants have been discovered for Hani Harb.