ORCID Profile
0000-0002-1722-8218
Current Organisation
National Institute of Water and Atmospheric Research Wellington
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Publisher: Informa UK Limited
Date: 28-06-2018
Publisher: Elsevier BV
Date: 06-2004
Publisher: CSIRO Publishing
Date: 2003
DOI: 10.1071/SB02025
Abstract: Extensive collections of Porphyra from throughout New Zealand since the early 1980s have revealed unexpectedly high ersity within the genus. From small-scale, opportunistic s ling of southern Australian Porphyra, small subunit ribosomal RNA gene (SSU) sequence data confirm the presence in Tasmania of a recently-described species of Porphyra, P. rakiura W.A.Nelson. This is the first report of P. rakiura from outside New Zealand. Analysis of rbcL–rbcS spacer DNA sequence from P. rakiura specimens and from an isotype specimen of P. lucasii Levring confirm that P. rakiura can be distinguished from the P. lucasii isotype at the molecular level as well as morphologically. A Western Australian specimen collected as P. lucasii provides a nuclear SSU sequence that is clearly different (92.1% similar) to that of P. rakiura, and rbcL–rbcS spacer sequence data identical to that of the P. lucasii isotype. Sequence data from the rbcL–rbcS spacer of a herbarium specimen from South Australia initially identified as P. lucasii, but with P. rakiura-like morphology, demonstrate that it is genetically distinct from P. rakiura and place it closer to, but not identical with, the P. lucasii isotype. This challenges the current taxonomic understanding of Porphyra in Australia, and of P. lucasii in particular.
Publisher: Elsevier BV
Date: 03-2008
DOI: 10.1016/J.YMPEV.2007.12.016
Abstract: A number of molecular studies of the Corallinales, a calcified order of the red algae, have used the conservative nSSU gene to investigate relationships within the order. However interspecific variation at this locus is low for closely related species, limiting resolution of recently erged groups. In this study, we obtained psbA sequence data from specimens of the order from New Zealand that had been identified according to current taxonomic criteria. We compared phylogenetic analyses based on psbA with those based on nSSU for the same dataset, and also analysed nSSU sequences of the New Zealand material with nSSU sequences of Corallinales taxa from other parts of the world. Our study shows that psbA has considerable potential as a marker for this group, being easily lified and considerably more variable than nSSU. Combined analyses using both markers provide significant support for relationships at both distal and terminal nodes of the analysis. Our analysis supports the monophyly of all three families currently defined in Corallinales: the Sporolithaceae, Hapalidiaceae and Corallinaceae, and indicates cryptic speciation in Mesophyllum and Spongites.
Publisher: Informa UK Limited
Date: 05-2004
Publisher: Wiley
Date: 05-04-2021
DOI: 10.1111/JPY.13115
Abstract: The coralline algal genus Corallinapetra is currently monospecific and was established on the species Corallinapetra novaezelandiae , known from a single collection from north‐eastern New Zealand. On the basis of multi‐gene phylogenetic analyses, Corallinapetra has been resolved apart from all currently recognized families and orders within the Corallinophycidae. We analyzed DNA sequence data from the holotype of Lithothamnion gabrielii , which has been considered a heterotypic synonym of L. muelleri , and an unidentified s le collected from Stewart Island in New Zealand, using psb A, rbc L, and COI‐5P genes. We also observed detailed morpho‐anatomical characters with light and scanning electron microscopy. Our phylogenetic analyses showed that L. gabrielii and the s le from New Zealand belonged to the same clade as Corallinapetra , distinct from other families and orders in the Corallinophycidae. Members of this clade are distinguishable from other families and orders in the Corallinophycidae by possessing sporangia that are surrounded by remnant sterile filaments that are weakly calcified in mature multiporate sporangial conceptacles that produce zonately ided tetrasporangia. Therefore, we propose that Corallinapetra be placed in its own family, Corallinapetraceae and order, Corallinapetrales, and that L. gabrielii should be assigned to Corallinapetra , as C. gabrielii , to reflect their phylogenetic relationships. We also obtained a partial rbc L sequence data from the lectotype of L. muelleri , the generitype of Lithothamnion . Comparison of the L. muelleri type sequence with L. gabrielii unambiguously demonstrated that these two species are not conspecific, and confirm the placement of L. muelleri within the Hapalidiales.
Publisher: Wiley
Date: 31-08-2022
Abstract: Advances in high-throughput sequencing (HTS) are revolutionizing monitoring in marine environments by enabling rapid, accurate and holistic detection of species within complex biological s les. Research institutions worldwide increasingly employ HTS methods for bio ersity assessments. However, variance in laboratory procedures, analytical workflows and bioinformatic pipelines impede the transferability and comparability of results across research groups. An international experiment was conducted to assess the consistency of metabarcoding results derived from identical s les and primer sets using varying laboratory procedures. Homogenized biofouling s les collected from four coastal locations (Australia, Canada, New Zealand and the USA) were distributed to 12 independent laboratories. Participants were asked to follow one of two HTS library preparation workflows. While DNA extraction, primers and bioinformatic analyses were purposefully standardized to allow comparison, many other technical variables were allowed to vary among laboratories ( lification protocols, type of instrument used, etc.). Despite substantial variation observed in raw results, the primary signal in the data was consistent, with the s les grouping strongly by geographical origin for all data sets. Simple post hoc data clean-up by removing low-quality s les gave the best improvement in s le classification for nuclear 18S rRNA gene data, with an overall 92.81% correct group attribution. For mitochondrial COI gene data, the best classification result (95.58%) was achieved after correction for contamination errors. The identified critical methodological factors that introduced the greatest variability (preservation buffer, s le defrosting, template concentration, DNA polymerase, PCR enhancer) should be of great assistance in standardizing future bio ersity studies using metabarcoding.
Publisher: Wiley
Date: 27-03-2015
DOI: 10.1111/JPY.12288
Abstract: Coralline red algae from the New Zealand region were investigated in a study focused on documenting regional ersity. We present a multi-gene analysis using sequence data obtained for four genes (nSSU, psaA, psbA, rbcL) from 68 s les. The study revealed cryptic ersity at both genus and species levels, confirming and providing further evidence of problems with current taxonomic concepts in the Corallinophycidae. In addition, a new genus Corallinapetra novaezelandiae gen. et sp. nov. is erected for material from northern New Zealand. Corallinapetra is excluded from all currently recognized families and orders within the Corallinophycidae and thus represents a previously unrecognized lineage within this subclass. We discuss rank in the Corallinophycidae and propose the order Hapalidiales.
Publisher: Informa UK Limited
Date: 05-2009
Publisher: Elsevier BV
Date: 09-2012
DOI: 10.1016/J.PHYTOCHEM.2012.06.003
Abstract: The coralline algae in the orders Corallinales and Sporolithales (subclass Corallinophycidae), with their high degree of mineralogical variability, pose a challenge to projections regarding mineralogy and response to ocean acidification. Here we relate skeletal carbonate mineralogy to a well-established phylogenetic framework and draw inferences about the effects of future changes in sea-water chemistry on these calcified red algae. A collection of 191 coralline algal specimens from New Zealand, representing 13 genera and 28 species, included members of three families: Corallinaceae, Hapalidiaceae, and Sporolithaceae. While most skeletal specimens were entirely calcitic (range: 73-100 wt.% calcite, mean 97 wt.% calcite, std dev=5, n=172), a considerable number contained at least some aragonite. Mg in calcite ranged from 10.5 to 16.4 wt.% MgCO(3), with a mean of 13.1 wt.% MgCO(3) (std dev=1.1, n=172). The genera Mesophyllum and Lithophyllum were especially variable. Growth habit, too, was related to mineralogy: geniculate coralline algae do not generally contain any aragonite. Mg content varied among coralline families: the Corallinaceae had the highest Mg content, followed by the Sporolithaceae and the Hapalidiaceae. Despite the significant differences among families, variation and overlap prevent the use of carbonate mineralogy as a taxonomic character in the coralline algae. Latitude (as a proxy for water temperature) had only a slight relationship to Mg content in coralline algae, contrary to trends observed in other biomineralising taxa. Temperate magnesium calcites, like those produced by coralline algae, are particularly vulnerable to ocean acidification. Changes in biomineralisation or species distribution may occur over the next few decades, particularly to species producing high-Mg calcite, as pH and CO(2) dynamics change in coastal temperate oceans.
Publisher: Wiley
Date: 13-09-0100
DOI: 10.1111/J.1529-8817.2011.01052.X
Abstract: The red algal order Bangiales has been revised as a result of detailed regional studies and the development of expert local knowledge of Bangiales floras, followed by collaborative global analyses based on wide taxon s ling and molecular analyses. Combined analyses of the nuclear SSU rRNA gene and the plastid RUBISCO LSU (rbcL) gene for 157 Bangiales taxa have been conducted. Fifteen genera of Bangiales, seven filamentous and eight foliose, are recognized. This classification includes five newly described and two resurrected genera. This revision constitutes a major change in understanding relationships and evolution in this order. The genus Porphyra is now restricted to five described species and a number of undescribed species. Other foliose taxa previously placed in Porphyra are now recognized to belong to the genera Boreophyllum gen. nov., Clymene gen. nov., Fuscifolium gen. nov., Lysithea gen. nov., Miuraea gen. nov., Pyropia, and Wildemania. Four of the seven filamentous genera recognized in our analyses already have generic names (Bangia, Dione, Minerva, and Pseudobangia), and are all currently monotypic. The unnamed filamentous genera are clearly composed of multiple species, and few of these species have names. Further research is required: the genus to which the marine taxon Bangia fuscopurpurea belongs is not known, and there are also a large number of species previously described as Porphyra for which nuclear SSU ribosomal RNA (nrSSU) or rbcL sequence data should be obtained so that they can be assigned to the appropriate genus.
Publisher: CSIRO Publishing
Date: 2010
DOI: 10.1071/SB09033
Abstract: The phylogenetic relationships of Porphyra collected from the Falkland Islands have been examined using molecular data from two loci, the plastid rbcL gene and the nuclear nSSU gene, in conjunction with morphological observations. Nine species have been distinguished, belonging to four distinct lineages within the Bangiales, differing from one another by 16–137 substitutions in the rbcL gene. One species is closely related to P. lissima, a north Atlantic taxon, three are resolved in a clade that includes several species from New Zealand, Australia and South Africa, as well as P. umbilicalis, whereas four others are more closely related to Southern Ocean species, including P. cinnamomea, P. virididentata and several as yet unnamed taxa from New Zealand, Australia and South Africa. Three species, one of which we identify as P. columbina, appear to be widely distributed in the Southern Ocean, with representatives on the Falkland Islands and on islands of the Australian and New Zealand subantarctic.
Location: New Zealand
Location: New Zealand
No related grants have been discovered for Judy Sutherland.