ORCID Profile
0000-0001-7552-0164
Current Organisations
Faculty of Sciences of the University of Lisbon
,
Universidade de Lisboa
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Publisher: MDPI AG
Date: 21-05-2015
Publisher: Public Library of Science (PLoS)
Date: 02-04-2013
Publisher: Elsevier BV
Date: 06-2013
DOI: 10.1016/J.PLAPHY.2013.02.024
Abstract: Grapevine (Vitis species) is the most economically important fruit crop worldwide. Ripening of non-climacteric fruits such as grapes has been the subject of intense research. Despite this interest, little is known on the role played by polyamines in the onset of ripening of non-climacteric fruits. These growth regulators have been involved in plant development and stress responses. Molecular and biochemical studies were developed in three important Portuguese cultivars (Trincadeira, Touriga Nacional and Aragonês) during the year 2008 and in Trincadeira during 2007 in order to gather insights on the role of polyamines in grape ripening. Microarray and real-time qPCR studies revealed up-regulation of a gene coding for arginine decarboxylase (ADC) during grape ripening in all the varieties. This increase was not accompanied by an increase in free and conjugated polyamines that presented a strong decrease. Putrescine and Spermidine levels were higher at earlier stages of development, while Spermine level remained constant. Berries of Trincadeira cultivar presented the highest content in total free and conjugated polyamines at earlier stages of fruit development in particular in the year 2007. The decrease in polyamines content during grape ripening was accompanied by up-regulation of genes coding for diamine oxidase (CuAO) and polyamine oxidase (PAO), together with a significant increase in their enzymatic activity and in the hydrogen peroxide content. These results provide, for the first time, strong evidence of a role of polyamine catabolism in grape ripening possibly through interaction with other growth regulators.
Publisher: Springer New York
Date: 28-10-2017
DOI: 10.1007/978-1-4939-7398-9_36
Abstract: Polyamines are small aliphatic amines that are found in both prokaryotic and eukaryotic organisms. These growth regulators have been implicated in abiotic and biotic stresses as well as plant development and morphogenesis. Several studies have also suggested a key role of polyamines during fruit set and early development. Polyamines have also been linked to fruit ripening and in the regulation of fruit quality-related traits.Recent studies indicate that during ripening of both climacteric and non-climacteric fruits, a decline in total polyamine contents is observed together with an increased catabolism of these growth regulators.In this review, we explore the current knowledge on polyamine biosynthesis and catabolism during fruit set and ripening. The study of the role of polyamine metabolism in fruit ripening indicates the possible application of these natural polycations to control ripening and postharvest decay as well as to improve fruit quality traits.
Publisher: Cold Spring Harbor Laboratory
Date: 11-09-2019
DOI: 10.1101/765594
Abstract: Phosphatidylinositol 3-phosphate (PtdIns3 P ) is one of the five different phosphoinositides (PPIs) species in plant cells, which regulate several aspects of plant growth and development, as well as responses to biotic and abiotic stresses. The mechanistic insights underlying PtdIns3 P mode of action, specifically through PtdIns3 P -binding effectors such as FYVE and PHOX proteins have been partially explored in plants with main focus on Arabidopsis thaliana. Additionally, they have been underexplored in other plant organisms such as bryophytes, the earliest erging group of terrestrial flora. In this study, we searched for genes coding for FYVE and PHOX domains containing sequences from different photosynthetic organisms in order to gather evolutionary insights on these PPI binding domains, followed by an in silico characterization of the FYVE and PHOX gene family in the moss Physcomitrella patens . Phylogenetic analysis showed that PpFYVE proteins can be grouped in 7 subclasses, with an additional subclass whose FYVE domain was lost during evolution to higher plants. On the other hand, PpPHOX proteins are classified into 5 subclasses. Expression analyses based on RNAseq data together with the analysis of cis -acting regulatory elements and transcription factor binding sites in promoter regions suggest the importance of these proteins in regulating stress responses but mainly developmental processes in P. patens . The results provide valuable information and robust candidate genes for future functional analysis aiming to further explore the role of this signaling pathway mainly during growth and development of tip growing cells and during the transition from 2D to 3D growth, which could provide ancestral regulatory players undertaken during plant evolution.
Publisher: Elsevier BV
Date: 2014
DOI: 10.1016/J.PLAPHY.2013.11.002
Abstract: Grapes are economically the most important fruit worldwide. However, the complexity of biological events that lead to ripening of nonclimacteric fruits is not fully understood, particularly the role of polyamines' catabolism. The transcriptional and metabolic profilings complemented with biochemical data were studied during ripening of Trincadeira grapes submitted to guazatine treatment, a potent inhibitor of polyamine oxidase activity. The mRNA expression profiles of one time point (EL 38) corresponding to harvest stage was compared between mock and guazatine treatments using Affymetrix GrapeGen(®) genome array. A total of 2113 probesets (1880 unigenes) were differentially expressed between these s les. Quantitative RT-PCR validated microarrays results being carried out for EL 35 (véraison berries), EL 36 (ripe berries) and EL 38 (harvest stage berries). Metabolic profiling using HPLC and (1)H NMR spectroscopy showed increase of putrescine, proline, threonine and 1-O-ethyl-β-glucoside in guazatine treated s les. Genes involved in amino acid, carbohydrate and water transport were down-regulated in guazatine treated s les suggesting that the strong dehydrated phenotype obtained in guazatine treated s les may be due to impaired transport mechanisms. Genes involved in terpenes' metabolism were differentially expressed between guazatine and mock treated s les. Altogether, results support an important role of polyamine catabolism in grape ripening namely in cell expansion and aroma development.
Publisher: Springer Science and Business Media LLC
Date: 2011
Publisher: Oxford University Press (OUP)
Date: 11-02-2015
DOI: 10.1093/JXB/ERU517
Location: Portugal
No related grants have been discovered for Ana Margarida Fortes.