ORCID Profile
0000-0001-6318-7384
Current Organisation
University of California, Irvine
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Publisher: Springer Science and Business Media LLC
Date: 10-03-2016
DOI: 10.1038/SREP22792
Abstract: Diagnosis and treatment of vocal fold lesions has been a long-evolving science for the otolaryngologist. Contemporary practice requires biopsy of a glottal lesion in the operating room under general anesthesia for diagnosis. Current in-office technology is limited to visualizing the surface of the vocal folds with fiber-optic or rigid endoscopy and using stroboscopic or high-speed video to infer information about submucosal processes. Previous efforts using optical coherence tomography (OCT) have been limited by small working distances and imaging ranges. Here we report the first full field, high-speed, and long-range OCT images of awake patients’ vocal folds as well as cross-sectional video and Doppler analysis of their vocal fold motions during phonation. These vertical-cavity surface-emitting laser source (VCSEL) OCT images offer depth resolved, high-resolution, high-speed, and panoramic images of both the true and false vocal folds. This technology has the potential to revolutionize in-office imaging of the larynx.
Publisher: Springer International Publishing
Date: 2018
Publisher: Mary Ann Liebert Inc
Date: 10-2001
DOI: 10.1089/107632701753213219
Abstract: Two-photon excitation laser scanning microscopy (TPM) was used to image human, porcine, and rabbit nasal septal cartilage. TPM provides optical sections of thick tissue specimens in situ without the use of exogenous dyes or need for tissue fixation. The cartilage tissue was imaged using near-infrared light generated by a mode-locked titanium/sapphire laser that was raster-scanned and coupled to an inverted microscope. Absorption of two photons by endogenous molecules and subsequent fluorescence was filtered to specific spectral bandwidths and detected with photomultiplier tubes. Two-photon stimulated fluorescence was detected with photomultiplier tubes optimized to specific spectral bandwidths. Signal intensity corresponds to the concentration of fluorophores, principally NADH, NADPH, and flavoproteins hence providing a means of redox imaging the cellular metabolic state. Specimens were scanned from the surface to a depth of about 150 microm. Image size was 50 x 50 microm with a diffraction limited pixel size of 0.4 microm. Cell membranes, nuclei, and matrix structures were identified in human, pig, and rabbit tissues. TPM provides a means to study three dimensional chondrocyte structure and matrix organization in situ at substantial depths, and permits longitudinal examination of cultured tissue explants without the need for exogenous dyes, tissue preparation, or fixation.
Publisher: Wiley
Date: 10-2002
Abstract: To evaluate the ability of devitalized cartilage to sustain repopulation by allogenic chondrocytes and to use 2‐photon microscopy to assess cultured chondrocyte viability. At an academic medical center, we conducted experimental analysis of lagomorph nasal septa devitalized by subjecting them to either 1) chemical dehydration in ethanol, 2) lyophilization (freeze dry), or 3) repeated freeze‐thaw (12 cycles) in liquid nitrogen. The processed cartilages were seeded with chondrocytes isolated from the septa of either the same rabbit or another rabbit and cultured for 4 weeks. They were assessed with a variety of techniques. The seeded chondrocytes maintained viability on the devitalized cartilage throughout the study period. Two‐photon microscopy was effective in assessing the viability of cultured chondrocytes. Isolated lagomorph chondrocytes can be maintained in allogenic cartilage grafts devitalized using conventional physicochemical techniques. This is the first experimental study to demonstrate that cultured chondrocytes can be maintained on devitalized cartilage.
No related grants have been discovered for Brian Wong.