ARDC Research Link Australia

ORCID Profile
Orcid icon. 0000-0002-8480-9813

Current Organisations
Northwestern University , Stanford University , University Hospital Rigshospitalet , IT University of Copenhagen , Statens Serum Institut , University of Greenland , Karolinska Institutet , Queen Ingrids Hospital , Imperial College London

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Publications

Publication

The New Arctic

Publisher: Springer International Publishing

Date: 2015

DOI: 10.1007/978-3-319-17602-4

Publication

Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling

Publisher: Oxford University Press (OUP)

Date: 03-2017

DOI: 10.1007/S10295-017-1904-5

Abstract: Heterologous protein production in the yeast Pichia pastoris can be limited by biological responses to high expression levels the unfolded protein response (UPR) is a key determinant of the success of protein production in this organism. Here, we used untargeted NMR metabolic profiling (metabolomics) of a number of different recombinant strains, carried out in a miniaturized format suitable for screening-level experiments. We identified a number of metabolites (from both cell extracts and supernatants) which correlated well with UPR-relevant gene transcripts, and so could be potential biomarkers for future high-throughput screening of large numbers of P. pastoris clones.

Publication

Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris

Publisher: Public Library of Science (PLoS)

Date: 18-03-2015

Location: United Kingdom of Great Britain and Northern Ireland

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Related Funding Activities

No related grants have been discovered for Rochelle Aw.

Rochelle Aw

Researcher

Related Links

Publications

The New Arctic

Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling

Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris

Related Organisations

Northwestern University

Stanford University

University Hospital Rigshospitalet

IT University Of Copenhagen

Statens Serum Institut

University Of Greenland

Karolinska Institutet

Queen Ingrids Hospital

ONERA Palaiseau

Fondation Mérieux

Imperial College London

Related Funding Activities

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