ORCID Profile
0000-0002-8651-965X
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Publisher: Frontiers Media SA
Date: 05-11-2021
DOI: 10.3389/FMICB.2021.761067
Abstract: The interplay between the compositional changes in the gastrointestinal microbiome, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) susceptibility and severity, and host functions is complex and yet to be fully understood. This study performed 16S rRNA gene-based microbial profiling of 143 subjects. We observed structural and compositional alterations in the gut microbiota of the SARS-CoV-2-infected group in comparison to non-infected controls. The gut microbiota composition of the SARS-CoV-2-infected in iduals showed an increase in anti-inflammatory bacteria such as Faecalibacterium ( p -value = 1.72 × 10 –6 ) and Bacteroides ( p -value = 5.67 × 10 –8 ). We also revealed a higher relative abundance of the highly beneficial butyrate producers such as Anaerostipes ( p -value = 1.75 × 10 –230 ), Lachnospiraceae ( p -value = 7.14 × 10 –65 ), and Blautia ( p -value = 9.22 × 10 –18 ) in the SARS-CoV-2-infected group in comparison to the control group. Moreover, phylogenetic investigation of communities by reconstructing unobserved state (PICRUSt) functional prediction analysis of the 16S rRNA gene abundance data showed substantial differences in the enrichment of metabolic pathways such as lipid, amino acid, carbohydrate, and xenobiotic metabolism, in comparison between both groups. We discovered an enrichment of linoleic acid, ether lipid, glycerolipid, and glycerophospholipid metabolism in the SARS-CoV-2-infected group, suggesting a link to SARS-CoV-2 entry and replication in host cells. We estimate the major contributing genera to the four pathways to be Parabacteroides , Streptococcus , Dorea , and Blautia , respectively. The identified differences provide a new insight to enrich our understanding of SARS-CoV-2-related changes in gut microbiota, their metabolic capabilities, and potential screening biomarkers linked to COVID-19 disease severity.
Publisher: Elsevier BV
Date: 08-2023
Publisher: Public Library of Science (PLoS)
Date: 22-09-2022
DOI: 10.1371/JOURNAL.PONE.0274961
Abstract: Coronavirus disease 2019 (COVID-19) was first identified in respiratory s les and was found to commonly cause cough and pneumonia. However, non-respiratory symptoms including gastrointestinal disorders are also present and a big proportion of patients test positive for the virus in stools for a prolonged period. In this cross-sectional study, we investigated viral load trends in stools and nasopharyngeal swabs and their correlation with multiple demographic and clinical factors. The study included 211 laboratory-confirmed cases suffering from a mild form of the disease and completing their isolation period at a non-hospital center in the United Arab Emirates. Demographic and clinical information was collected by standardized questionnaire and from the medical records of the patient. Of the 211 participants, 25% tested negative in both s le types at the time of this study and 53% of the remaining patients had detectable viral RNA in their stools. A positive fecal viral test was associated with male gender, diarrhea as a symptom, and hospitalization during infection. A positive correlation was also observed between a delayed onset of symptoms and a positive stool test. Viral load in stools positively correlated with, being overweight, exercising, taking antibiotics in the last 3 months and blood type O. The viral load in nasopharyngeal swabs, on the other hand, was higher for blood type A, and rhesus positive (Rh factor). Regression analysis showed no correlation between the viral loads measured in stool and nasopharyngeal s les in any given patient. The results of this work highlight the factors associated with a higher viral count in each s le. It also shows the importance of stool s le analysis for the follow-up and diagnosis of recovering COVID-19 patients.
Publisher: Elsevier BV
Date: 04-2021
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Mariane Daou.