ORCID Profile
0000-0002-2153-8956
Current Organisation
Southern Cross University
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Publisher: Springer Science and Business Media LLC
Date: 25-02-2009
Publisher: Springer Science and Business Media LLC
Date: 06-06-2007
Publisher: Public Library of Science (PLoS)
Date: 19-02-2009
Publisher: The Royal Society
Date: 18-03-2008
Abstract: The symbiosis between reef-building corals and their algal endosymbionts (zooxanthellae of the genus Symbiodinium ) is highly sensitive to temperature stress, which makes coral reefs vulnerable to climate change. Thermal tolerance in corals is known to be substantially linked to the type of zooxanthellae they harbour and, when multiple types are present, the relative abundance of types can be experimentally manipulated to increase the thermal limits of in idual corals. Although the potential exists for this to translate into substantial thermal acclimatization of coral communities, to date there is no evidence to show that this takes place under natural conditions. In this study, we show field evidence of a dramatic change in the symbiont community of Acropora millepora , a common and widespread Indo-Pacific hard coral species, after a natural bleaching event in early 2006 in the Keppel Islands (Great Barrier Reef). Before bleaching, 93.5% ( n =460) of the randomly s led and tagged colonies predominantly harboured the thermally sensitive Symbiodinium type C2, while the remainder harboured a tolerant Symbiodinium type belonging to clade D or mixtures of C2 and D. After bleaching, 71% of the surviving tagged colonies that were initially C2 predominant changed to D or C1 predominance. Colonies that were originally C2 predominant suffered high mortality (37%) compared with D-predominant colonies (8%). We estimate that just over 18% of the original A. millepora population survived unchanged leaving 29% of the population C2 and 71% D or C1 predominant six months after the bleaching event. This change in the symbiont community structure, while it persists, is likely to have substantially increased the thermal tolerance of this coral population. Understanding the processes that underpin the temporal changes in symbiont communities is key to assessing the acclimatization potential of reef corals.
Publisher: Public Library of Science (PLoS)
Date: 26-07-2016
Publisher: Wiley
Date: 03-05-2005
Publisher: Frontiers Media SA
Date: 07-09-2018
Publisher: Public Library of Science (PLoS)
Date: 04-2021
DOI: 10.1371/JOURNAL.PONE.0242633
Abstract: Cannabis sativa has been cultivated since antiquity as a source of fibre, food and medicine. The recent resurgence of C . sativa as a cash crop is mainly driven by the medicinal and therapeutic properties of its resin, which contains compounds that interact with the human endocannabinoid system. Compared to other medicinal crops of similar value, however, little is known about the biology of C . sativa . Glandular trichomes are small hair-like projections made up of stalk and head tissue and are responsible for the production of the resin in C . sativa . Trichome productivity, as determined by C . sativa resin yield and composition, is only beginning to be understood at the molecular level. In this study the proteomes of glandular trichome stalks and heads, were investigated and compared to the proteome of the whole flower tissue, to help further elucidate C . sativa glandular trichome biochemistry. The data suggested that the floral tissue acts as a major source of carbon and energy to the glandular trichome head sink tissue, supplying sugars which drive secondary metabolite biosynthesis. The trichome stalk seems to play only a limited role in secondary metabolism and acts as both source and sink.
Publisher: Springer Science and Business Media LLC
Date: 2013
Publisher: Oxford University Press (OUP)
Date: 22-07-2014
DOI: 10.1093/JXB/ERU299
Publisher: Wiley
Date: 26-01-2022
DOI: 10.1002/JRS.6308
Abstract: Commercial tea tree oil (TTO) has to conform chemically to the international standard ISO4730: 2017, which defines acceptable ranges for the 15 main terpenoids therein. Lack of in‐house capacity for the technology typically required for terpenoid quantification (GC‐FID) necessitates that Australian TTO producers outsource the analyses to commercial service providers. This is associated with high cost and slow turnaround times, which have been recognized as limiting factors for TTO process optimization in the areas of quality assurance, distillation and blending. This paper describes the testing of two custom portable Raman devices with different lasers (785 and 1064 nm) for the relative quantification of the 15 main TTO components using model‐based predictions. Initial testing showed that the 1064‐nm device provided superior data for TTO predictions. Machine‐learning algorithms were trained with spectral data from the 1064‐nm device and corresponding GC‐FID data from 214 TTO s les. Hold‐over validation (HV) correlations between actual and predicted values using up to 53 unseen s les showed r 2 at or above 0.92 for 10 and at or above 0.95 for 6 of the 15 specified terpenoids. The highly abundant key TTO quality compound terpinen‐4‐ol showed an HV r 2 of 0.96 with a root mean square error (RMSE) of 0.37%, whereas the lowly abundant key compound 1,8‐cineole showed an HV r 2 of 1.00 with a RMSE of 0.13%. These results indicated a strong potential for Raman spectrometry to provide real‐time product quality data at TTO distillation sites enabling direct feedback control and process optimisations.
Publisher: Public Library of Science (PLoS)
Date: 24-07-2009
Publisher: MDPI AG
Date: 08-12-2017
Publisher: Wiley
Date: 16-06-2004
Publisher: Wiley
Date: 2009
DOI: 10.1111/J.1755-0998.2008.02222.X
Abstract: Understanding the flexibility of the endosymbioses between scleractinian corals and single-cell algae of the genus Symbiodinium will provide valuable insights into the future of coral reefs. Here, a real-time polymerase chain reaction (PCR) assay is presented to accurately determine the cell densities of Symbiodinium clades C and D in the scleractinian coral Acropora millepora, which can be extended to other coral-symbiont associations in the future. The assay targets single- to low-copy genes of the actin family of both the coral host and algal symbiont. Symbiont densities are expressed as the ratio of Symbiodinium cells to each host cell (S/H ratio, error within 30%), but can also be normalized to coral surface area. Greater accuracy in estimating ratios of associations involving multiple clades is achieved compared with previous real-time PCR assays based on high-copy ribosomal DNA loci (error within an order of magnitude). Healthy adult A. millepora containing ~1.4 × 10(6) zooxanthellae per cm(2) (as determined by haemocytometer counts) had S/H ratios of c. 0.15, i.e. ~15 symbiont cells per 100 host cells. In severely bleached colonies, this ratio decreased to less than 0.005. Because of its capacity to accurately determine both densities and ratios of multiple symbionts within one s le, the assay will open the door for novel research into the mechanisms of symbiont shuffling and switching.
No related grants have been discovered for Jos Mieog.