ORCID Profile
0000-0002-7197-2101
Current Organisations
Westfälische Wilhelms-Universität Münster
,
Julius-Maximilians-Universität Würzburg
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Publications
Stalk cell polar ion transport provide for bladder‐based salinity tolerance in Chenopodium quinoa
Publisher: Wiley
Date: 06-2022
DOI: 10.1111/NPH.18205
Abstract: Chenopodium quinoa uses epidermal bladder cells (EBCs) to sequester excess salt. Each EBC complex consists of a leaf epidermal cell, a stalk cell, and the bladder. Under salt stress, sodium (Na + ), chloride (Cl − ), potassium (K + ) and various metabolites are shuttled from the leaf lamina to the bladders. Stalk cells operate as both a selectivity filter and a flux controller. In line with the nature of a transfer cell, advanced transmission electron tomography, electrophysiology, and fluorescent tracer flux studies revealed the stalk cell’s polar organization and bladder‐directed solute flow. RNA sequencing and cluster analysis revealed the gene expression profiles of the stalk cells. Among the stalk cell enriched genes, ion channels and carriers as well as sugar transporters were most pronounced. Based on their electrophysiological fingerprint and thermodynamic considerations, a model for stalk cell transcellular transport was derived.
Venus Flytrap HKT1-Type Channel Provides for Prey Sodium Uptake into Carnivorous Plant Without Conflicting with Electrical Excitability
Publisher: Elsevier BV
Date: 03-2016
Calcium sensor kinase activates potassium uptake systems in gland cells of Venus flytraps
Publisher: Proceedings of the National Academy of Sciences
Date: 21-05-2015
Abstract: The Venus flytrap Dionaea muscipula has been in the focus of scientists since Darwin’s time. Carnivorous plants, with their specialized lifestyle, including insect capture, as well as digestion and absorption of prey, developed unique tools to gain scarce nutrients. In this study, we identified the molecular nature of the uptake machinery for prey-derived potassium and the posttranslational regulation. For the first time, to our knowledge, we functionally characterize DmHAK5 here—a KUP/HAK/KT family member as activated by a CBL-CIPK kinase complex. Detailed electrophysiological characterization identified DmHAK5 as a proton-driven, high-affinity potassium transporter with a weak selectivity. Working hand-in-hand with the low-affinity, high-capacity K + -channel DmKT1 activated by the same kinase, the transporters allow the Venus flytrap to take up prey-derived potassium.
Understanding the Molecular Basis of Salt Sequestration in Epidermal Bladder Cells of Chenopodium quinoa
Publisher: Elsevier BV
Date: 10-2018
DOI: 10.1016/J.CUB.2018.08.004
Abstract: Soil salinity is destroying arable land and is considered to be one of the major threats to global food security in the 21st century. Therefore, the ability of naturally salt-tolerant halophyte plants to sequester large quantities of salt in external structures, such as epidermal bladder cells (EBCs), is of great interest. Using Chenopodium quinoa, a pseudo-cereal halophyte of great economic potential, we have shown previously that, upon removal of salt bladders, quinoa becomes salt sensitive. In this work, we analyzed the molecular mechanism underlying the unique salt dumping capabilities of bladder cells in quinoa. The transporters differentially expressed in the EBC transcriptome and functional electrophysiological testing of key EBC transporters in Xenopus oocytes revealed that loading of Na
Insect haptoelectrical stimulation of Venus flytrap triggers exocytosis in gland cells
Publisher: Proceedings of the National Academy of Sciences
Date: 17-04-2017
Abstract: The Venus flytrap has been in the focus of scientists since Darwin’s time. Carnivorous plants, with their specialized lifestyle, including insect capture, as well as digestion and absorption of prey, developed unique tools to gain scarce nutrients. In this study, we describe mechanistic insights into the cascade of events following the capture of insect prey. Action potentials evoked by the struggling prey are translated into touch-inducible hormone signals that promote the formation of secretory vesicles. Different varieties of digestive compounds are released sequentially into the flytrap’s “green stomach” and break down the captured animal. Amperometry provides insight into the kinetics and chemistry of the stimulus-coupled glandular secretion process.
Signaling and transport processes related to the carnivorous lifestyle of plants living on nutrient-poor soil
Publisher: Oxford University Press (OUP)
Date: 22-06-2021
Abstract: In Eukaryotes, long-distance and rapid signal transmission is required in order to be able to react fast and flexibly to external stimuli. This long-distance signal transmission cannot take place by diffusion of signal molecules from the site of perception to the target tissue, as their speed is insufficient. Therefore, for adequate stimulus transmission, plants as well as animals make use of electrical signal transmission, as this can quickly cover long distances. This update summarises the most important advances in plant electrical signal transduction with a focus on the carnivorous Venus flytrap. It highlights the different types of electrical signals, examines their underlying ion fluxes and summarises the carnivorous processes downstream of the electrical signals.
Sugar beet cold-induced PMT5a and STP13 carriers are poised for taproot proton-driven plasma membrane sucrose and glucose import
Publisher: Cold Spring Harbor Laboratory
Date: 22-09-2021
DOI: 10.1101/2021.09.21.461191
Abstract: As the major sugar-producing crop in the northern hemisphere, sugar beet taproots store sucrose at a concentration of about 20 %. While the vacuolar sucrose loader TST has already been identified in the taproot, sugar transporters mediating sucrose uptake across the plasma membrane of taproot parenchyma cells remained unknown. We electrophysiologically examined taproots for proton-coupled sugar uptake and identified potentially involved transporters by transcriptomic profiling. After cloning, the transporter features were studied in the heterologous Xenopus laevis oocyte expression system using the two-electrode voltage cl technique. Insights into the structure were gained by 3D homology modeling. As with glucose, sucrose stimulation of taproot parenchyma cells caused inward H + -fluxes and plasma membrane depolarization, indicating a sugar roton symport mechanism. As one potential candidate for sugar uploading, the BvPMT5a was characterized as a H + -driven low-affinity glucose transporter, which does not transport sucrose. BvSTP13 operated as a high-affinity H + /sugar symporter, transporting glucose and to some extent sucrose due to a binding cleft plasticity. Both transporter genes were upregulated upon cold exposure, with the transport capacity of BvSTP13 being more cold-resistant than BvPMT5a. Identification of BvPMT5a and BvSTP13 as taproot sugar transporters could improve breeding of cold-tolerant sugar beet to provide a sustainable energy crop.
The Venus Flytrap Dionaea muscipula Counts Prey-Induced Action Potentials to Induce Sodium Uptake
Publisher: Elsevier BV
Date: 02-2016
Related Organisations
Westfälische Wilhelms-Universität Münster
Location: No location found
Related Funding Activities
No related grants have been discovered for Sönke Scherzer.