ARDC Research Link Australia

ORCID Profile
Orcid icon. 0000-0002-6467-7024

Current Organisation
Howard Hughes Medical Institute Janelia Farm Research Campus

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Publications

Publication

Publisher Correction: Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution

Publisher: Springer Science and Business Media LLC

Date: 04-12-2020

DOI: 10.1038/S41592-019-0695-1

Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Publication

Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution

Publisher: Springer Science and Business Media LLC

Date: 18-11-2020

DOI: 10.1038/S41592-019-0641-2

Abstract: We demonstrate gas cluster ion beam scanning electron microscopy (SEM), in which wide-area ion milling is performed on a series of thick tissue sections. This three-dimensional electron microscopy technique acquires datasets with <10 nm isotropic resolution of each section, and these can then be stitched together to span the sectioned volume. Incorporating gas cluster ion beam SEM into existing single-beam and multibeam SEM workflows should be straightforward, increasing reliability while improving z resolution by a factor of three or more.