ORCID Profile
0000-0002-6010-4130
Current Organisation
Badan Riset dan Inovasi Nasional Republik Indonesia
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Publisher: Informa UK Limited
Date: 02-01-2023
Publisher: MDPI AG
Date: 08-11-2022
DOI: 10.3390/SU142214720
Abstract: Peatlands in Indonesia are one of the world’s largest carbon sinks, helping to regulate greenhouse gas emissions and global climate change. Lophostemon suaveolens is a relatively unexplored plant found in Papua’s endemic peat ecosystem that grows well in wet areas with low fertility. It is geographically dispersed and has the potential for peatland rehabilitation. Seed is one of materials for the reproduction of L. suaveolens. However, the difficulty in seed collection and the limitation in seed production has become a current problem for its cultivation. Seed multiplication by using an in vitro method would be one of the mechanisms to overcome the problem. We present an efficient and reproducible protocol for in vitro multiplication of plantlets using nodal segments and shoot apices collected from plantlets. After 3 months of the culture initiation stage, the elongated axillary shoots were separated from the clumps and further multiplied using Murashige and Skoog (MS) media supplemented with (1) BAP (0.5 mL/L) as single PGR, (2) NAA (0.1 mL/L) as a single PGR, and (3) a combination of two types of PGR BAP (0.5 mL/L) and NAA (0.1 mL/L). Up to an incubation period of 6 months, the efficiency of leaf axillary shoot propagation was determined by counting the number of nodule multiplication coefficient (NMC), shoot length, root length, and number of leaves (six consecutive subcultures). The higher the NMC, the higher the plantlets obtained, increasing shoot regeneration from nodules physiologically increasing evapotranspiration in vitro. The highest of NMC (8.4) was observed in MS medium with a combination of 0.5 mL/L BAP and 0.1 mL/L NAA (double PGRs), with the longest shoots (5.91 cm), the longest root length (8.83 cm), and the most leaves (32). When a combination of BAP and NAA were used simultaneously, the plantlets during acclimatization were the highest survived. It was concluded that MS in combination with 0.5 mL/L BAP and 0.1 mL/L NAA is the most appropriate protocol for the success of in vitro multiplication of L. suaveolens. This is the first report of L. suaveolens in vitro multiplication, and the protocol could be used to propagate this peatland species on a large scale. The authors acknowledge the limitations of the experimental work and recommend further work to increase the s le size and complete the field-testing phase to help verify the initial findings presented in this paper.
Publisher: Elsevier BV
Date: 10-2019
Publisher: American Chemical Society (ACS)
Date: 04-2003
DOI: 10.1021/JF020981U
Abstract: The identification of Melaleuca cajuputi leaf s les (trees) that demonstrate enhanced oil characteristics using near infrared (NIR) spectroscopy is described. Leaf s les from an unthinned M. cajuputi seedling seed orchard in Indonesia were collected and air-dried, and their 1,8-cineole content and oil concentrations were determined. NIR spectra of the leaves were obtained, and calibrations for 1,8-cineole content and oil concentration were developed using spectra that had been selected using spectral features that is, no knowledge of 1,8-cineole content or oil concentration was used to select the calibration s les. The calibrations were used to predict the 1,8-cineole content and oil concentration of the remaining s les. It was demonstrated that NIR spectroscopy could be used to identify leaf s les that had high 1,8-cineole contents and oil concentrations. The technique has the potential to greatly reduce the time involved in ranking large numbers of s les for these attributes, as is a requirement in tree breeding programs to enhance oil production.
Publisher: Springer Science and Business Media LLC
Date: 25-03-2022
DOI: 10.1007/S11295-022-01548-9
Abstract: Three screening trials of clonally replicated Acacia mangium seedlings were evaluated for survival and lesion length following inoculation with locally collected strains of Ceratocystis in Indonesia. Tolerance in the population was low with 6.7% of the 1033 clones represented by more than 4 ramets surviving repeated inoculations. Differences in tolerance among populations were slight however, populations with consistently higher survival and shorter lesion lengths were from Papua New Guinea rather than Queensland. Estimates of the proportion of the experimental variation attributable to differences among parents (heritability) were low to moderate for both survival and lesion length. Estimates of the proportion of the experimental variation that was attributable to differences among clones (repeatability) were greater but typically similar to the heritability estimates, indicating that initial improvements from selection will primarily be derived from identifying tolerant parents. While genetic correlations among experiments were positive, estimates could not exclude the existence of host–pathogen interactions. Two validation trials of the tolerant clones were assessed 9 months after establishment these trials verified that one-third of the clones identified in the nursery screening were also tolerant to Ceratocystis in field trials. The experiments confirmed that nursery screening may be used to quickly focus efforts on parents that produce more tolerant progeny, screening additional seedlings to increase selection intensity rather than using clonal replication to increase accuracy would lead to greater improvements in tolerance and field trials are required to verify disease tolerance at later ages.
Publisher: Springer Science and Business Media LLC
Date: 22-05-2019
Publisher: Informa UK Limited
Date: 03-04-2021
Publisher: IOP Publishing
Date: 02-2020
DOI: 10.1088/1755-1315/449/1/012038
Abstract: Cajuput tree is native to Maluku and produce 1,8 cineole based essential oil. Since 1995, the Centre of Biotechnology and Tree Improvement has implemented a genetic improvement program of cajuput aiming to increase oil yield and oil content. The program has successfully produced superior seeds with the yield of 2%. In early 2016, a small scale pilot plantation of cajuput was established in 5 ha community land di K ung Rimbajaya Distrik Biak Timur Kabupaten Biak, Papua. Leaf distillation started at the end of 2017 using a simple distillation unit. Previously, the local farmers were woodcutters and shifting cultivators. After the establishment of cajuput plantation, they became permanent farmers and cajuput oil refiners. Leaf distillation of genetically improved trees produced oil yield of 1.3%, a significant improvement from the average of 0.6 – 0.8% yield of unimproved trees. This 5 ha plantation of 2500 trees/ha potentially produce 400 kg/year. Some of the cajuput oil is sold in a local market in 8ml bottle. This cajuput oil production scheme has increased the farmers welfare. Furthermore, the success story of Kovarwis farmers inspired other farmers surrounding Rimbajaya to develop similar plantation. When this small-scale plantation is replicated in other parts of Indonesia, cajuput oil production will increase and Indonesia may one day become self-sufficient in cajuput oil. An agreement with cajuput packaging company is being establish to ensure that the oil produce by farmers will be purchased by the company and thus ensuring steady supply of good quality oil.
Publisher: Elsevier BV
Date: 2007
Publisher: Informa UK Limited
Date: 03-04-2019
Publisher: Oxford University Press (OUP)
Date: 11-2016
Publisher: Springer Science and Business Media LLC
Date: 25-07-2017
Publisher: Informa UK Limited
Date: 08-06-2023
Publisher: Elsevier BV
Date: 11-2015
Publisher: Informa UK Limited
Date: 05-10-2023
Publisher: IOP Publishing
Date: 07-2021
DOI: 10.1088/1755-1315/800/1/012041
Abstract: Clonal seed orchard (CSO) is established from superior trees vegetative material to produce the genetically improved seed. The flowering pattern and number of clone in CSO influence seed quality, genetic ersity and the possibility of inbreeding. The study aimed to determine the flowering pattern, identify effective population size and effective clone size in cajuput CSO. This was conducted in cajuput CSO in two sites, i.e. Gunungkidul and Sleman, during peak flowering. The cajuput CSO was established in a randomized complete block design using 30 clones in Sleman and Gunungkidul with five and eight replications, respectively. The flowering number of each in idual, number of flowering ramet for each clone and number of capsule retention were recorded. The result showed that the clone performance (i.e., flowering and survival rate) in Sleman was better than in Gunungkidul. Two clones (i.e., number 14 of Provenance Suli, Ambon, and number 30 of Provenance Masarete, Moluccas) in Gunungkidul did not produce flower seed. The effective population size in Gunungkidul (Ne=26.20) is higher than in Sleman (Ne=25). However, the effective clone number in Gunungkidul (Nc=25) is lower than in Sleman (Nc=27). Genetic ersity in both CSOs is high (0.98). Therefore, the proportional mixing of seed collected from both CSO is essential to increase the genetic ersity and the realized genetic gain in deploying seeds for operational planting.
Publisher: IOP Publishing
Date: 07-2021
DOI: 10.1088/1755-1315/800/1/012040
Abstract: Melaleuca cajuput (cajuput) subsp. cajuputi is an endemic species of Indonesia, found naturally in the island of Buru, Seram and Ambon and cultivated in Java. The cajuputi tree is harvested for the leaves which contain important essential oil of 1,8 cineole. Because of intensive utilization and inappropriate management of the resources, the genetic ersity in the natural distribution is under threat. Collection of genetic materials across its natural distribution conducted in 1995 and 1998 had captured more than 160 genotypes for screening of high oil yield and 1,8 cineole content. The selected genotypes had been planted as seed orchards in Java for production of genetically improved seeds. Breeding program is continuing to produce elite clones. The use of superior seeds/clones for plantation is the best solution to increase cajuput oil production and to achieve self-sufficiency in oil supply. Both industrial scale and smallholder plantations are being established to increase the national production which currently stood at around 650 ton/year whilst domestic demand is already over 3500 ton/year. The species is a good ex le of successful domestication. The native stands are still utilized for oil production and plantations outside the natural range are developing. The paper also discusses importance of breeding program to increase cajuputi oil production in Indonesia.
Location: Indonesia
No related grants have been discovered for Anto Rimbawanto.