ORCID Profile
0000-0002-2641-8646
Current Organisation
National Research and Innovation Agency
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Publisher: Agency for Marine and Fisheries Research and Development
Date: 04-05-2015
DOI: 10.15578/SQUALEN.V10I1.132
Abstract: Chitosan-starch blend film showed desirable elongation and oxygen transmission rate (OTR) values and demonstrated rapid degradation in soil burial treatment. The incorporation of higher glycerol concentration lowered tensile strength but increased the amount of transmittable water vapour. The biodegradability of chitosan-starch based film was tested using soil burial test. Chitosan-starch blend films were able to degrade naturally at slightly similar period, ranging from 72–87 days. The addition of glycerol was likely contributed to the high Water Vapour Transmission Rate (WVTR) value which faster the biodegradability of the film in soil. Among different type of acid solutions, lactic acid is less preferable solution to dissolve chitosan as it produced film with poor mechanical properties compared to acetic and formic acid. Based on its mechanical limitation, the film can be used as a coating based film for certain type of fish product, especially product with high lipid content.
Publisher: Elsevier BV
Date: 09-2016
DOI: 10.1016/J.MARPOLBUL.2016.05.008
Abstract: The Jakarta Bay Ecosystem is located in the vicinity of the megacity Jakarta, the capital city of Indonesia. Surrounding rivers and canals, carrying solid and fluid waste from households and several industrial areas, flow into the bay. Therefore, the levels of selected trace hazardous elements in water, surface sediments and animal tissues were determined. S les were collected from two different seasons. The spatial distribution pattern of trace elements in sediment and water as well as the seasonal variation of the contamination were assessed. Quality assessment of sediment using the effects range median (ERM) showed that the concentrations of Hg, Cu and Cr at some stations exceeded the recommended values. Moreover, the concentrations of several trace hazardous elements in the sediments exceeded previously reported toxicity thresholds for benthic species.
Publisher: EDP Sciences
Date: 2022
DOI: 10.1051/E3SCONF/202234405001
Abstract: Histamine fish poisoning (HFP) is one of many global food safety issues experienced by fish industries in both developed and developing countries, in which temperature abuse and mishandling during processing, storage, and distribution were identified as the main sources of histamine formation and accumulation in the products. In Indonesia, official reports on HFP are limited, however, mass media documented HFP cases and outbreaks which occurred every year, from different regions of Indonesia. The Scombroid fish, including Bullet and Frigate Tuna locally named as Tongkol ( Auxis rochei, A. thazard ), was reported as the main food vehicle causing the majority of HFP cases. The maximum allowable level of histamine for fresh and processed fish marketed in Indonesia is 100 mg/kg, except for fresh tuna for sashimi (50 mg/kg), based on Indonesian National Standard (SNI). The results from official control and monitoring programs by Indonesian’s government as well as published studies reported the presence of elevated levels of histamine in fish sold at domestic markets, hence implementing the cold-chain system during post-harvest stages is still a challenge. Evaluation of the current national requirement for histamine testing for fish products also important, to ensure product safety prior to consumption.
Publisher: Agency for Marine and Fisheries Research and Development
Date: 27-08-2020
DOI: 10.15578/SQUALEN.V15I2.444
Abstract: Pathogenic bacteria are commonly found as natural contaminants in seafood and fish products. Globally, several countries have been imposing strict regulations on the maximum levels of pathogens and consequently require microbial testing of pathogens before the products can be marketed. A culture-based method with biochemical assay has been widely used to detect pathogenic bacteria in food, despite its long and extensive process. Meanwhile, the alternative molecular-based method to overcome this problem, cannot differentiate between viable and nonviable cells, which may lead to underestimation. This study aimed to develop a multiplex PCR (mPCR) method as a confirmatory assay for the culture-based method to detect pathogens in fish products simultaneously. This method applied a pre-enrichment step to ensure the growth of low-level pathogens and the injured cells in the s le. The target genes were ToxR, InvA, and UidA for Vibrio parahaemolyticus, Salmonella spp. and Escherichia coli, respectively. This assay also lified the 16S rDNA gene of bacteria as an internal control for the PCR reaction. By implementing liquid-based DNA extraction during analysis, the developed-mPCR was comparable to detect the targeted bacteria in artificially-contaminated s les. The method was more sensitive in naturally-contaminated s les, where the number of E. coli, Salmonella spp. and V. parahaemolyticus detected were 28, 7, and 22, respectively. While the conventional method only detected 26, 5, and 19 of the respective pathogens. With a relatively shorter time and lower operation cost, the mPCR method is potential as an alternative for the culture-based method.
Publisher: Elsevier BV
Date: 02-2021
DOI: 10.1016/J.IJFOODMICRO.2021.109459
Abstract: Indonesian salted-boiled fish (pindang) is a popular traditional food in Indonesia, which is made from Scombroid fish such as tuna and mackerel. As with other traditionally prepared fish products, pindang has important economic and social values, especially for those living in the coastal areas of Indonesia. However, pindang is a major cause of histamine fish poisoning (HFP) for consumers. Klebsiella aerogenes T124, a relatively high histamine-producing isolate from pindang, was used to describe lag time (λ), growth rate (μ
No related grants have been discovered for Radestya Triwibowo.