ORCID Profile
0000-0002-4727-1882
Current Organisations
IT University of Copenhagen
,
Københavns Universitet
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Publisher: Oxford University Press (OUP)
Date: 11-2018
Abstract: Background: As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the in idual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing. Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of A. vera, and they have been widely studied in the past decades. However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions, as most of them require a complete or near-complete fractionation of the polymers. Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll. Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes. Results: Preliminary findings showed that CoMPP can successfully be used for high-throughput screening of aloe leaf mesophyll tissue. Seventeen species of Aloe and closely related genera were analyzed, and a clear difference in the polysaccharide compositions of the mesophyll tissues was seen. Conclusions: These preliminary data suggest that the polysaccharides vary between species and that true species of Aloe may differ from segregate genera.
Publisher: Det Kgl. Bibliotek/Royal Danish Library
Date: 14-11-2022
Abstract: Aloe vera kendes af mange fra blomsterhandlerens potteplanter, apotekets håndkøbsprodukter eller supermarkedets sæbe- og cremesortiment. Planten er allestedsnærværende og kan beskrives som en nutidig kommerciel superstjerne. Aloe vera tilhører planteslægten Aloe, og er blot én ud af over 600 forskellige arter i denne slægt. Omkring 25% af aloerne har været brugt eller bruges i traditionel medicin i de områder, hvor de er naturligt forekommende. Slægten Aloe omtales i de tidligste medicinske skrifter, og aloerne er blevet brugt, delt, studeret og transporteret af både lægfolk og lærde folk igennem årtusinder. Det er tankevækkende, at én planteart er kommet til at dominere et kommercielt marked i en sådan grad, som Aloe vera gør i dag. De mange Aloe vera produkter på hylderne er dog et fint eksempel på et naturvidenskabeligt indhold pakket ind i en kulturhistorisk fortælling, der ligesom plantens egen historie repræsenterer en tæt sammenvævning af natur- og kulturhistorie.
Publisher: Frontiers Media SA
Date: 14-05-2019
Publisher: Georg Thieme Verlag KG
Date: 14-12-2016
Publisher: Wiley
Date: 19-02-2023
DOI: 10.1002/PPP3.10361
Abstract: Aloes are iconic succulent plants native to Africa, Madagascar and the Arabian Peninsula. The succulent leaf mesophyll of aloes has been used extensively as a herbal product for centuries, contributing to their overexploitation. Health benefits are attributed to their polysaccharide content. We present a comprehensive comparison of the polysaccharide composition of succulent tissues from 93 Aloe species. We found polysaccharide composition primarily related to leaf morphology in alignment with the broad range of Aloe species used medicinally. All aloes except Aloe ferox and Aloe vera are endangered raising concern about over‐harvesting of wild species. Aloes are iconic succulent plants native to Africa, Madagascar and the Arabian Peninsula. All aloes except the commercially grown Aloe ferox and Aloe vera are protected according to the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). Major factors contributing to their over‐exploitation are their ornamental value and medicinal use with more than 25% of Aloe species being utilised. The succulent inner leaf mesophyll of aloes is used in traditional medicine, with the healing effect ascribed to the properties of their structural polysaccharides. To explore the correlation between Aloe polysaccharide profiles and other biologically relevant traits across the genus, we (1) extracted polysaccharides and created profiles for nearly 100 representative species using carbohydrate microarrays and molecular probes. We targeted six major plant cell wall polysaccharide groups using 27 different molecular probes. We (2) tested for phylogenetic signal in the polysaccharide profiles and (3) assembled an exhaustive database from literature on the geographic region, level of endemism, altitude, habitat, habit, medicinal use and leaf morphology of the in idual species of Aloe . In the absence of phylogenetic signal of polysaccharide profiles, multivariate linear modelling without phylogenetic correction was used and showed that polysaccharide composition primarily correlated with leaf morphology, highlighting the fundamental role of polysaccharides as the building blocks of plants. No correlations between polysaccharide composition of commercial and non‐commercial species were found. We found polysaccharide composition to primarily relate to leaf morphology emphasising the fundamental and structural role of polysaccharides.
Publisher: Elsevier BV
Date: 02-2023
Publisher: Elsevier BV
Date: 04-2014
Publisher: Wiley
Date: 03-06-2019
DOI: 10.1111/PCE.13560
Publisher: Elsevier BV
Date: 05-2015
DOI: 10.1016/J.CARRES.2015.03.015
Abstract: Pectin-one of the most complex biomacromolecules in nature has been extensively studied using various techniques. This has been done so in an attempt to understand the chemical composition and conformation of pectin, whilst discovering and optimising new industrial applications of the polymer. For the last decade the emergence of glycan microarray technology has led to a growing capacity of acquiring simultaneous measurements related to various carbohydrate characteristics while generating large collections of data. Here we used a multivariate analysis approach in order to analyse a set of 359 pectin s les probed with 14 different monoclonal antibodies (mAbs). Principal component analysis (PCA) and partial least squares (PLS) regression were utilised to obtain the most optimal qualitative and quantitative information from the spotted microarrays. The potential use of microarray technology combined with chemometrics for the accurate determination of degree of methyl-esterification (DM) and degree of blockiness (DB) was assessed.
Location: Denmark
Start Date: 2021
End Date: 2024
Funder: Torben og Alice Frimodts Fond
View Funded ActivityStart Date: 2021
End Date: 2024
Funder: Hartmann Fonden
View Funded ActivityStart Date: 2022
End Date: 2024
Funder: Villum Fonden
View Funded Activity