ORCID Profile
0000-0003-1088-0569
Current Organisations
Osaka Metropolitan University
,
Kyoto University
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Publisher: Elsevier BV
Date: 08-1999
DOI: 10.1016/S0021-9673(99)00504-X
Abstract: An application study of an on-line concentration technique of neutral analytes for micellar electrokinetic chromatography (MEKC) was carried out in environmental analysis to enhance the UV detection sensitivity. Several dioxins and related compounds, such as dibenzofuran, dibenzo-p-dioxin, 2,3- and 2,7-dichlorodibenzo-p-dioxins, and 2,3,7-trichlorodibenzo-p-dioxin, were used as test solutes. For a highly sensitive separation and detection, cyclodextrin-modified MEKC (CD-MEKC) under acidic conditions was employed as a separation mode and stacking using reverse migrating micelles and a water plug (SRW) as an on-line concentration technique. Almost a 200-fold gain in detection sensitivity was obtained for the model compounds in SRW-CD-MEKC compared to that in normal CD-MEKC without on-line concentration and the limit of detection was found to be around 0.1 ppm for each solute.
Publisher: Elsevier BV
Date: 05-2001
DOI: 10.1016/S0021-9673(01)00566-0
Abstract: Two on-line s le concentration techniques, s le stacking and sweeping, were evaluated using cationic surfactants as pseudostationary phases in micellar electrokinetic chromatography. As cationic surfactant micelles, tetradecyltrimethylammonium bromide and cetyltrimethylammonium chloride were employed. About 10-fold and 1000-fold increases in detection sensitivity in terms of peak heights were observed by s le stacking and sweeping, respectively, without suppression of the electroosmotic flow. In particular, the concentration limits of detection (S/N=3) for test naphthalenesulfonic acids obtained with sweeping were from 0.96 to 0.47 ppb with UV detection without any preconcentration procedure.
Publisher: Elsevier BV
Date: 04-1999
Publisher: Elsevier BV
Date: 12-2001
DOI: 10.1016/S0021-9673(01)01346-2
Abstract: The separation and on-line preconcentration by sweeping of charged analytes in electrokinetic chromatography using a neutral pseudostationary phase is described. Under neutral or basic conditions, the electrophoretic migration of anionic analytes towards the anode is overcome by a high, cathode-directed electroosmotic flow hence, they experience net migration towards the cathode, and the system is run at positive polarity mode. The separation and the retention factor, k, are dependent on both the analyte's electrophoretic mobility and its interaction with the pseudostationary phase. The versatility of the sweeping mechanism is then shown in this system. The charged analyte, prepared in a matrix free of the pseudostationary phase, penetrates the pseudostationary phase zone upon application of voltage. Analyte molecules are consequently accumulated and concentrated. As a demonstration, the separation and preconcentration of phenol derivatives using nonionic surfactants of the alkyl polyoxyethylene ether type (Brij 35 and Brij 58) yielded peak height enhancements up to 100-fold. The efficiency of s le stacking was also found to be improved with the use of a high viscosity background solution.
Publisher: Elsevier BV
Date: 08-1998
DOI: 10.1016/S0378-4347(98)00063-2
Abstract: Utility of a second enhanced field zone (water zone) is investigated for the on-line concentration of neutral analytes in micellar electrokinetic chromatography. Micellar solutions of sodium dodecyl sulfate prepared in acidic phosphate buffers are used as separation and s le solutions. Prior to long hydrodynamic injection of s les prepared in a low conductivity matrix, a long water plug is hydrodynamically injected to provide a second enhanced field zone. Practical and some fundamental considerations are presented. The technique is selective towards hydrophobic analytes. Notable detector response improvements (>100-fold) for several analytes are observed experimentally.
Location: No location found
No related grants have been discovered for Koji Otsuka.