ORCID Profile
0000-0002-0075-4481
Current Organisations
University of Technology Sydney
,
University of Aberdeen
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Communications Technologies | Interdisciplinary Engineering not elsewhere classified | Microwave and Millimetrewave Theory and Technology |
Expanding Knowledge in Engineering | Expanding Knowledge in Technology
Publisher: Optica Publishing Group
Date: 02-09-2008
DOI: 10.1364/OE.16.014550
Abstract: We present the use of optical fibers to form a counter-propagating optical trap as a means of manipulating both solid and liquid aerosols. We explore the use of single and multimode fibers to achieve trapping of various particles in air, present the trapping properties of the different fiber types and compare the observed trends to those predicted by theory. Using fibers, we are able to hold suspended particles for extended periods of time and to precisely manipulate them over distances of several hundred microns. We discuss the difficulties and advantages of each fiber configuration and conclude with a demonstration that fiber based trapping offers a good candidate for studying optical binding in air.
Publisher: The Optical Society
Date: 25-11-2014
DOI: 10.1364/OL.39.006691
Publisher: SPIE
Date: 31-08-2006
DOI: 10.1117/12.677222
Publisher: SPIE
Date: 18-10-2004
DOI: 10.1117/12.555663
Publisher: American Physical Society (APS)
Date: 10-10-2000
Publisher: Optica Publishing Group
Date: 25-02-2010
Publisher: SPIE
Date: 08-09-2011
DOI: 10.1117/12.903618
Publisher: IEEE
Date: 06-2007
Publisher: OSA
Date: 2014
Publisher: IEEE
Date: 2003
Publisher: American Chemical Society (ACS)
Date: 14-12-2015
Abstract: Using blends of bioethanol and gasoline as automotive fuel leads to a net decrease in the production of harmful emission compared to the use of pure fossil fuel. However, fuel droplet evaporation dynamics change depending on the mixing ratio. Here we use single particle manipulation techniques to study the evaporation dynamics of ethanol/gasoline blend microdroplets. The use of an electrodynamic balance enables measurements of the evaporation of in idual droplets in a controlled environment, while optical tweezers facilitate studies of the behavior of droplets inside a spray. Hence, the combination of both methods is perfectly suited to obtain a complete picture of the evaporation process. The influence of adding varied amounts of ethanol to gasoline is investigated, and we observe that droplets with a greater fraction of ethanol take longer to evaporate. Furthermore, we find that our methods are sensitive enough to observe the presence of trace amounts of water in the droplets. A theoretical model, predicting the evaporation of ethanol and gasoline droplets in dry nitrogen gas, is used to explain the experimental results. Also a theoretical estimation of the saturation of the environment, with other aerosols, in the tweezers is carried out.
Publisher: Wiley
Date: 22-08-2017
Abstract: Prostate cancer is a multifocal disease with characteristic heterogeneity and foci that can range from low grade indolent to aggressive disease. The latter is characterised by the well-established histopathological Gleason grading system used in the current clinical care. Nevertheless, a large discrepancy exists on initial biopsy and after the final radical prostatectomy. Moreover, there is no reliable imaging modality to study these foci, in particular at the level of the cells and surrounding matrix. Extracellular matrix (ECM) remodelling is significant in cancer progression with collagen as the dominant structural component providing mechanical strength and flexibility of tissue. In this study, the collagen assembly in prostate tissue was investigated with second harmonic generation (SHG) microscopy: malignant foci demonstrated a reticular pattern, with a typical collagen pattern for each Gleason score. The orientation of collagen for each biopsy was computed by applying a ratio of the anisotropic and isotropic collagen fibres. This value was found to be distinct for each Gleason score. The findings suggest that this approach can not only be used to detect prostate cancer, but also can act as a potential biomarker for cancer aggressiveness.
Publisher: Royal Society of Chemistry (RSC)
Date: 2009
DOI: 10.1039/B914165K
Abstract: Micron and sub-micron sized aerosol particles are captured, manipulated and characterised in a Bessel beam optical trap. Bright field microscopy and elastic light scattering measurements are used in combination to interrogate trapped particles and explore the optical landscape of the trap. We conclude that the Bessel trap has a number of advantages over optical tweezers in terms of characterisation of accumulation mode particles, manipulation of particles over macroscopic length scales and effective control of the gas phase. As such, the Bessel trap is a valuable addition to the aerosol optical toolkit.
Publisher: SPIE
Date: 03-03-2022
DOI: 10.1117/12.2607692
Publisher: IOP Publishing
Date: 22-10-2004
Publisher: Optica Publishing Group
Date: 2020
DOI: 10.1364/CLEOPR.2020.C1G_3
Abstract: To simplify the reconstruction algorithms in ghost imaging, we present a feedback-based approach to reduce reconstruction times. We introduce a genetic algorithm to optimize the illumination patterns in real-time to match with the object’s shape.
Publisher: IEEE
Date: 2000
Publisher: American Physical Society (APS)
Date: 09-0010
Publisher: IOP Publishing
Date: 07-04-2017
Abstract: It is perhaps surprising that something as fragile as a microscopic droplet could possibly form a laser. In this article we will review some of the underpinning physics as to how this might be possible, and then examine the state of the art in the field. The technology to create and manipulate droplets will be examined, as will the different classes of droplet lasers. We discuss the rapidly developing fields of droplet biolasers, liquid crystal laser droplets and explore how droplet lasers could give rise to new bio and chemical sensing and analysis. The challenges that droplet lasers face in becoming robust devices, either as sensors or as photonic components in the lab on chip devices, is assessed.
Publisher: OSA
Date: 2011
Publisher: AIP Publishing
Date: 04-2011
DOI: 10.1063/1.3579499
Abstract: Acoustic cavitation can occur in therapeutic applications of high- litude focused ultrasound. Studying acoustic cavitation has been challenging, because the onset of nucleation is unpredictable. We hypothesized that acoustic cavitation can be forced to occur at a specific location using a laser to nucleate a microcavity in a pre-established ultrasound field. In this paper we describe a scientific instrument that is dedicated to this outcome, combining a focused ultrasound transducer with a pulsed laser. We present high-speed photographic observations of laser-induced cavitation and laser-nucleated acoustic cavitation, at frame rates of 0.5×106 frames per second, from laser pulses of energy above and below the optical breakdown threshold, respectively. Acoustic recordings demonstrated inertial cavitation can be controllably introduced to the ultrasound focus. This technique will contribute to the understanding of cavitation evolution in focused ultrasound including for potential therapeutic applications.
Publisher: Elsevier BV
Date: 10-2003
Publisher: Springer Science and Business Media LLC
Date: 05-04-2019
DOI: 10.1038/S41598-019-42008-0
Abstract: Worldwide, prostate cancer sits only behind lung cancer as the most commonly diagnosed form of the disease in men. Even the best diagnostic standards lack precision, presenting issues with false positives and unneeded surgical intervention for patients. This lack of clear cut early diagnostic tools is a significant problem. We present a microfluidic platform, the Time-Resolved Hydrodynamic Stretcher (TR-HS), which allows the investigation of the dynamic mechanical response of thousands of cells per second to a non-destructive stress. The TR-HS integrates high-speed imaging and computer vision to automatically detect and track single cells suspended in a fluid and enables cell classification based on their mechanical properties. We demonstrate the discrimination of healthy and cancerous prostate cell lines based on the whole-cell, time-resolved mechanical response to a hydrodynamic load. Additionally, we implement a finite element method (FEM) model to characterise the forces responsible for the cell deformation in our device. Finally, we report the classification of the two different cell groups based on their time-resolved roundness using a decision tree classifier. This approach introduces a modality for high-throughput assessments of cellular suspensions and may represent a viable application for the development of innovative diagnostic devices.
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 10-2021
Publisher: Optica Publishing Group
Date: 04-12-2009
DOI: 10.1364/OE.17.023316
Publisher: SPIE
Date: 28-05-2004
DOI: 10.1117/12.533746
Publisher: American Physical Society (APS)
Date: 17-11-2010
Publisher: Optica Publishing Group
Date: 10-07-2006
DOI: 10.1364/OE.14.006373
Abstract: We characterize the ability of Gaussian and Bessel beams to guide water, ethanol and dodecane aerosol droplets. Droplets produced from a nebuliser source are trapped using radiation pressure and then by varying the beam power are controllably guided in a vertical direction. The use of a zeroth-order Bessel beam, which has a non-diffracting thin core, is shown to improve guiding distances over a comparable Gaussian beam by more than three times with guiding distances of up to 2.75mm for dodecane droplets. We discuss the applications for this work in the context of tools for optically manipulating airborne particles.
Publisher: The Optical Society
Date: 25-03-2019
Publisher: Springer Science and Business Media LLC
Date: 12-2012
DOI: 10.1038/492051A
Publisher: SPIE
Date: 02-02-2019
DOI: 10.1117/12.794195
Publisher: Optica Publishing Group
Date: 14-05-2008
DOI: 10.1364/OE.16.007739
Abstract: We demonstrate a method for the optical trapping of solid aerosol particles. Suspension of silica particles in ethanol allows their delivery to the trapping volume using a commercial medical nebulizer. The ethanol quickly evaporates, leaving the solid particles trapped in air. We use the technique to make comparisons between aerosol and colloid tweezing through power spectra analysis of the particle's positions fluctuations for identical particles trapped in a water or air suspending medium.
Publisher: Cold Spring Harbor Laboratory
Date: 29-05-2019
DOI: 10.1101/653394
Abstract: In stimulated emission depletion (STED) nanoscopy, the major origin of decreased signal-to-noise ratio within images can be attributed to s le photobleaching and strong optical aberrations. This is due to STED utilising both a high power depletion laser (increasing risk of photodamage), while the depletion beam is very sensitive to s le-induced aberrations. Here we demonstrate a custom-built 3D STED microscope with automated aberration correction that is capable of 3D super-resolution imaging through thick, highly aberrating, tissue. We introduce and investigate image denoising by block-matching and collaborative filtering (BM3D) to numerically enhance fine object details otherwise mixed with noise. Numerical denoising provides an increase in the final effective resolution of the STED imaging of 31% using the well-established Fourier ring correlation metric. Experimental validation of the proposed method is achieved through super-resolved 3D imaging of axons in differentiated induced pluripotent stem cells growing under a 80µm thick layer of tissue with lateral and axial resolution of 256nm and 300nm, respectively.
Publisher: arXiv
Date: 2014
Publisher: Optica Publishing Group
Date: 03-2000
DOI: 10.1364/OL.25.000341
Abstract: We report extended mode-hop-free tuning in a continuous-wave, pump-enhanced optical parametric oscillator (PE-OPO). We employ a dual-cavity configuration to allow independent control of the resonant pump and signal fields, and so we can suppress frequent mode hops in the signal as the pump is tuned in frequency. With the signal field cl ed in frequency by an uncoated etalon, the idler field can be scanned smoothly through a range of 10.8 GHz. The PE-OPO outputs can also be tuned coarsely from 1.01 to 1.18 mum in the signal and from 2.71 to 3.26 mum and 4.07 to 5.26 mum in the idler. We find that increased idler absorption only slightly increases the oscillation threshold.
Publisher: Informa UK Limited
Date: 2021
Publisher: IOP Publishing
Date: 24-07-2007
Publisher: OSA
Date: 2019
Publisher: SPIE
Date: 06-2015
DOI: 10.1117/12.2179201
Publisher: Royal Society of Chemistry (RSC)
Date: 2011
DOI: 10.1039/C1LC20541B
Abstract: We demonstrate the combination of a rails and anchors microfluidic system with laser forcing to enable the creation of highly controllable 2D droplet arrays. Water droplets residing in an oil phase can be pinned to anchor holes made in the base of a microfluidic channel, enabling the creation of arrays by the appropriate patterning of such holes. The introduction of laser forcing, via laser induced thermocapillary forces to anchored droplets, enables the selective extraction of particular droplets from an array. We also demonstrate that such anchor arrays can be filled with multiple, in our case two, droplets each and that if such droplets have different chemical contents, the application of a laser at their interface triggers their merging and a chemical reaction to take place. Finally by adding guiding rails within the microfluidic structure we can selectively fill large scale arrays with monodisperse droplets with significant control over their contents. In this way we make a droplet array filled with 96 droplets containing different concentrations of fluorescent microparticles.
Publisher: Informa UK Limited
Date: 10-03-2006
Publisher: Elsevier BV
Date: 03-2018
Publisher: IEEE
Date: 09-2009
Publisher: American Physical Society (APS)
Date: 05-07-2007
Publisher: Proceedings of the National Academy of Sciences
Date: 30-09-2020
Abstract: Cells confined in complex environments use a combination of chemical and mechanical cues for robust pathfinding and effective migration. Analysis of directional “decision-making” of Dictyostelium discoideum cells migrating within microchannels harboring asymmetric bifurcations shows that unlike neutrophils and immature dendritic cells Dictyostelium cells use chemical rather than barotactic guidance cues. Cells in steeper adenosine 3′,5′-cyclic monophosphate gradients migrating at higher speeds split their leading edges more readily when confronted with a bifurcation in the channel. The point at which one of the competing pseudopods starts to retract appears to be dependent on a relative force imbalance between two competing pseudopods, showing that cellular mechanics plays a major role in leading-edge dynamics, including front splitting, polarization, and retraction in D. discoideum .
Publisher: The Optical Society
Date: 07-03-2016
DOI: 10.1364/BOE.7.001193
Publisher: Optica Publishing Group
Date: 31-01-2023
DOI: 10.1364/JOSAB.468638
Abstract: Quantifying the size range of aerosols that can be trapped in a counterpropagation dual-fiber trapping configuration is important in understanding how these particles can be manipulated and characterized in such traps. Here, we present simulations and experiments investigating the trapped aerosol size range variations in the intermediate position of two fibers under different fiber separations, aerosol particle sizes, fiber powers, and radial offset. By doing so, we establish a parametric space plot of stable aerosol trapping, and the parametric analysis provides insight into the tolerance of such traps to trapping fluctuations.
Publisher: American Physical Society (APS)
Date: 25-02-2004
Publisher: SPIE
Date: 08-09-2011
DOI: 10.1117/12.893346
Publisher: SPIE
Date: 13-09-2007
DOI: 10.1117/12.733716
Publisher: Wiley
Date: 23-11-2018
Publisher: Cold Spring Harbor Laboratory
Date: 14-01-2020
DOI: 10.1101/2020.01.14.904748
Abstract: Neutrophils and dendritic cells have, besides their well characterised chemotactic movement responses, been shown to be able to detect and respond to local differences in hydraulic resistance ( barotaxis ). Furthermore, for neutrophils, it has been suggested that barotaxis overrides chemotaxis. Here, we investigate whether Dictyostelium cells also respond to hydraulic resistance or primarily to chemical gradients using an asymmetric bifurcating micro-channel. This channel design allows us to decouple hydraulic and chemical stimuli, by providing a choice between moving up a chemical gradient or down a chemical gradient into a channel with 100 times lower hydraulic resistance. Under these conditions chemotaxis always overrides barotaxis. Cells confronted by a microchannel bifurcation are observed to often partially split their leading edge and to start moving into both channels. Cells in steeper cAMP gradients, that move faster, split more readily. The decision to retract the pseudopod moving away from the cAMP source is made when the average velocity of the pseudopod moving up the cAMP gradient is 20% higher than the average velocity of the pseudopod moving down the gradient. Surprisingly, this decision threshold is independent of the steepness of the cAMP gradient and speed of movement. It indicates that a critical force imbalance threshold underlies the repolarisation decision. We investigate the directional ‘decision-making’ of Dictyostelium discoideum cells migrating within engineered micro-channels harbouring asymmetric bifurcations. Unlike neutrophils and immature dendritic cells, Dictyostelium cells strongly prioritise chemical over barotactic guidance cues. Cells in steeper cAMP gradients migrate at higher speeds, split their leading edges more readily when confronted with a bifurcation in the channel. The decision to retract a pseudopod pointing in an unfavourable direction occurs when a critical tension gradient between two competing pseudopods is surpassed. These experiments show that although barotaxis is not a major guidance cue, cellular mechanics plays a major role in leading edge dynamics, including front splitting and polarisation and retraction.
Publisher: SPIE
Date: 28-08-2008
DOI: 10.1117/12.794855
Publisher: Springer Science and Business Media LLC
Date: 27-08-2015
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 06-2020
Publisher: AIP Publishing
Date: 19-09-2006
DOI: 10.1063/1.2336772
Abstract: We demonstrate that optical tweezers can be used to control and characterize the coagulation and mixing state of aerosols. Liquid aerosol droplets of 2–14μm in diameter are optically trapped and characterized by spontaneous and stimulated Raman scatterings, which together provide a unique signature of droplet size and composition. From the conventional bright field image, the size of the trapped droplet can be estimated and compared with that determined from stimulated Raman scattering, and the motion of the particle within the trapping plane can be recorded. A maximum of four droplets can be manipulated in tandem by forming multiple optical traps through rapid beam steering. The coagulation of two droplets can be studied directly by controlling two droplets. The limiting conditions under which optical forces and capillary forces dominate the aerosol coagulation event are explored by varying the relative optical trap strengths and characterizing the coagulation of different droplet sizes. Finally, we demonstrate that the coagulation of different aerosol components can be compared and the mixing state of the final coagulated droplet can be investigated. In particular, we compare the outcome of the coagulation of an aqueous sodium chloride aerosol droplet with a second aqueous droplet, with an ethanol droplet or with a decane droplet.
Publisher: John Wiley & Sons, Inc
Date: 29-04-2018
Publisher: SPIE
Date: 16-09-2016
DOI: 10.1117/12.2239025
Publisher: American Physical Society (APS)
Date: 12-07-2007
Publisher: SPIE
Date: 31-08-2006
DOI: 10.1117/12.683635
Publisher: Informa UK Limited
Date: 09-2000
Publisher: Elsevier BV
Date: 02-2015
Publisher: OSA
Date: 2003
Publisher: Optica Publishing Group
Date: 2007
DOI: 10.1364/OE.15.006330
Abstract: Degenerate four-wave mixing is demonstrated using an artificial Kerr medium and is evidenced by directly observing the phase conjugation of a vortex signal beam. The nonlinear susceptibility is produced by a refractive index grating created in a suspension of dielectric microscopic particles optically confined in the intensity grating distribution of two interfering laser beams.
Publisher: SPIE
Date: 16-09-2014
DOI: 10.1117/12.2061378
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 12-2021
Publisher: IEEE
Date: 06-2017
Publisher: IEEE
Date: 06-2007
Publisher: The Optical Society
Date: 05-2015
Publisher: Royal Society of Chemistry (RSC)
Date: 2009
DOI: 10.1039/B904690A
Abstract: The time-dependent evolution in the equilibrium size of an optically trapped aqueous sodium chloride droplet (>2 microm radius) within an environment of varying relative humidity (RH) is shown to depend on both the depression in vapour pressure due to the presence of the solute and the elevation in temperature due to optical absorption. In particular, the level of optical absorption is highly dependent on the size of the droplet relative to the wavelength of the absorbed light. Thus, as the droplet size tunes into a Mie resonance at the trapping laser wavelength, the increased level of optical absorption leads to an elevation in droplet temperature. This increase in resonant heating can balance a continual increase in RH, leading to only marginal growth in droplet size and change in solute concentration. Once the RH is sufficiently high that the resonance condition can be surpassed, the droplet cools instantaneously and the solute concentration again dominates in determining the vapour pressure, with a rapid increase in size and a decrease in solute concentration returning the droplet to equilibrium with the gas phase RH. Thus, a growing droplet is observed to pass through periods of apparent size stability followed by instantaneous growth, consistent with the variation in absorption efficiency with droplet size. This provides a clear ex le of the coupling between the optical and physical properties of an aerosol and their influence on the equilibrium state.
Publisher: Wiley
Date: 06-2008
Publisher: Optica Publishing Group
Date: 15-07-2008
DOI: 10.1364/OE.16.011411
Abstract: The vortex emergence process as an integer order Bessel field progresses continuously onto the contiguous higher order Bessel field is studied in detail. We assess the progressive migration of phase singularities and explain the predicted increase in fractional orbital angular momentum content of the beam in terms of this gradual process.
Publisher: IOP Publishing
Date: 19-05-2009
Publisher: SPIE
Date: 18-10-2004
DOI: 10.1117/12.556319
Publisher: American Society of Mechanical Engineers
Date: 16-11-2020
Abstract: Direct low flow sensing is of interest to many applications in medical and biochemical industries. Low flow rate measurement is still challenging, and conventional flow sensors such as hot films, hot wires and Pitot probes are not capable of measuring very low flow rates accurately. In some applications that require flow measurement in a small diameter tubing (e.g. intravenous (IV) infusion), using such sensors also becomes mechanically impractical. Herein, a flexible laser-induced graphitic (LIG) piezoresistive flow sensor has been fabricated in a cost-effective single processing step. The capability of the LIG sensor in very low flow rate measurement has been investigated by embedding the sensor within an intravenous (IV) line. The embedded LIG hair-like sensor was tested at ambient temperature within the IV line at flow rates ranging from 0 m/s to 0.3 m/s (IV infusion free-flow rate). The LIG hair-like sensor presented in this study detects live flow rates of IV infusions with a threshold detection limit as low as 0.02 m/s. Moreover, the deformation of the LIG hair-like sensor that lead to resistance change in response to various flow rates is simulated using COMSOL Multiphysics.
Publisher: OSA
Date: 2011
Publisher: arXiv
Date: 2009
Publisher: American Chemical Society (ACS)
Date: 14-02-2013
DOI: 10.1021/NL304607V
Abstract: Axially resolved microphotoluminescence mapping of semiconductor nanowires held in an optical tweezers reveals important new experimental information regarding equilibrium trapping points and trapping stability of high aspect ratio nanostructures. In this study, holographic optical tweezers are used to scan trapped InP nanowires along the beam direction with respect to a fixed excitation source and the luminescent properties are recorded. It is observed that nanowires with lengths on the range of 3-15 μm are stably trapped near the tip of the wire with the long segment positioned below the focus in an inverted trapping configuration. Through the use of trap multiplexing we investigate the possibility of improving the axial stability of the trapped nanowires. Our results have important implication for applications of optically assisted nanowire assembly and optical tweezers based scanning probes microscopy.
Publisher: The Optical Society
Date: 03-09-2015
DOI: 10.1364/BOE.6.003757
Publisher: Royal Society of Chemistry (RSC)
Date: 2012
DOI: 10.1039/C2CP42925J
Abstract: In this paper we explore the trapping of aerosol droplets using an annular beam, formed by blocking the central portion of a Gaussian beam, and quantify the improvements over conventional Gaussian beam traps. Recent work on the modelling of single aerosol dynamics within an optical tweezer trap [Burnham et al., Journal of the Optical Society of America B, 2011, 28, 2856-2864] has indicated that the use of annular beams can allow smaller droplets to be trapped, which we experimentally verify. We also demonstrate that annular beams allow droplets to be trapped at higher powers, and with reduced axial displacement with increasing power, than Gaussian beams. We confirm these results, due to a reduction in the axial scattering forces, using this theoretical model. Finally back focal plane interferometry is used to determine the axial and lateral trap stiffnesses for a series of droplets, showing a significant increase in the axial : lateral trap stiffness ratio from 0.79 ± 0.04 to 1.15 ± 0.04 when an annular beam is used.
Publisher: Elsevier BV
Date: 04-2001
Publisher: The Company of Biologists
Date: 2020
DOI: 10.1242/DEV.175109
Abstract: Directional cell intercalations of epithelial cells during gastrulation has in several organisms been shown to be associated with a planar cell polarity in the organisation of the actin-myosin cytoskeleton and is postulated to reflect directional tension that drives oriented cell intercalations. We have characterised and applied a recently introduced non-destructive optical manipulation technique to measure the tension in in idual epithelial cell junctions of cells in various locations and orientations in the epiblast of chick embryos in the early stages of primitive streak formation. Junctional tension of mesendoderm precursors in the epiblast is higher in junctions oriented in the direction of intercalation than in junctions oriented perpendicular to the direction of intercalation and higher than in junctions of other cells in the epiblast. The kinetic data are fitted best with a simple visco-elastic Maxwell model and we find that junctional tension and to a lesser extent viscoelastic relaxation time are dependent on myosin activity.
Publisher: OSA
Date: 2003
Publisher: IEEE
Date: 06-2007
Publisher: SPIE
Date: 09-10-2012
DOI: 10.1117/12.928558
Publisher: Optica Publishing Group
Date: 12-05-2008
DOI: 10.1364/OE.16.007655
Abstract: We report on the optical trapping of water droplets with a supercontinuum laser source. Droplet size is determined by observing the spectrum of the on-axis backscattered light. In contrast to to monochromatic trapping, the broad spectrum of the supercontinuum covers several resonances of the first excited Mie coefficients. A minimum value of Q approximately 0.16 for the trapping efficiency is estimated.
Publisher: Informa UK Limited
Date: 2005
Publisher: American Chemical Society (ACS)
Date: 16-12-2020
Abstract: In stimulated emission depletion (STED) nanoscopy, the major origin of decreased signal-to-noise ratio within images can be attributed to s le photobleaching and strong optical aberrations. This is due to STED utilizing a high-power depletion laser (increasing the risk of photodamage), while the depletion beam is very sensitive to s le-induced aberrations. Here, we demonstrate a custom-built STED microscope with automated aberration correction that is capable of 3D super-resolution imaging through thick, highly aberrating tissue. We introduce and investigate a state of the art image denoising method by block-matching and collaborative 3D filtering (BM3D) to numerically enhance fine object details otherwise mixed with noise and further enhance the image quality. Numerical denoising provides an increase in the final effective resolution of the STED imaging of 31% using the well established Fourier ring correlation metric. Results achieved through the combination of aberration correction and tailored image processing are experimentally validated through super-resolved 3D imaging of axons in differentiated induced pluripotent stem cells growing under an 80 μm thick layer of tissue with lateral and axial resolution of 204 and 310 nm, respectively.
Publisher: Elsevier BV
Date: 12-2002
Publisher: IEEE
Date: 2000
Publisher: SPIE
Date: 13-09-2007
DOI: 10.1117/12.736448
Publisher: American Physical Society (APS)
Date: 06-1999
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C5RA11431D
Abstract: A low-cost optical manipulation system is realised by using simple microfabricated PDMS components coupled to a smartphone camera for imaging.
Publisher: IEEE
Date: 06-2017
Publisher: Optica Publishing Group
Date: 15-04-2003
DOI: 10.1364/OL.28.000657
Abstract: We examine the properties of interfering high-order Bessel beams. We implement an experimental setup that allows us to realize these interferograms, using interfering Laguerre-Gaussian beams and an axicon. We demonstrate the use of such beams for controlled rotation of microscopic particles in optical tweezers and rotators. The self-healing properties of interfering Bessel beams allow the simultaneous manipulation and rotation of particles in spatially separated s le cells.
Publisher: OSA
Date: 2008
Publisher: SPIE
Date: 28-07-2017
DOI: 10.1117/12.2284553
Publisher: Royal Society of Chemistry (RSC)
Date: 2009
DOI: 10.1039/B814545H
Abstract: Arrays of optically tweezed aerosol droplets, each of sub-picolitre volume, are manipulated by holographic optical tweezers and characterised by cavity enhanced Raman spectroscopy. A spatial light modulator is employed to generate arrays of optical traps from a single laser beam and to control the array dimensions and relative trap positions. Comparative hygroscopicity measurements are performed concurrently on five trapped droplets by monitoring the evolving size of each droplet. This is extended to the controlled coalescence of an array of droplets accompanied by spectroscopic measurements. These data represent the first ever simultaneous measurements of the evolving composition and size of an array of aerosol droplets. We consider the possibility of using aerosol arrays as a platform for studying chemical reactions in sub-picolitre volumes, exploiting the versatility of aerosol arrays for performing optical digital microfluidic operations accompanied by micro-total analysis.
Publisher: The Optical Society
Date: 11-2013
DOI: 10.1364/BOE.4.002710
Publisher: The Royal Society
Date: 18-10-2006
Abstract: In 1986, Arthur Ashkin and colleagues published a seminal paper in Optics Letters , ‘Observation of a single-beam gradient force optical trap for dielectric particles’ which outlined a technique for trapping micrometre-sized dielectric particles using a focused laser beam, a technology which is now termed optical tweezers. This paper will provide a background in optical manipulation technologies and an overview of the applications of optical tweezers. It contains some recent work on the optical manipulation of aerosols and concludes with a critical discussion of where the future might lead this maturing technology.
Publisher: AIP Publishing
Date: 11-2004
DOI: 10.1063/1.1814820
Abstract: A vertically oriented zero order Bessel light beam is shown to create a one-dimensional array of trapped particles over extended (millimeter) distances. The particles take up equilibrium positions over the entire length of the beam and this is a consequence of the interplay between optical scattering and the self-healing properties of the Bessel beam. This work has analogies to recent studies of optically bound matter and allows for the simple creation of one-dimensional particle chains and their subsequent spectroscopic analysis.
Publisher: IOP Publishing
Date: 20-04-2004
Publisher: SPIE
Date: 18-08-2005
DOI: 10.1117/12.614416
Publisher: Informa UK Limited
Date: 09-1998
Publisher: Optica Publishing Group
Date: 29-12-2003
DOI: 10.1364/OE.11.003562
Abstract: We demonstrate the use of a spatial light modulator (SLM) to facilitate the trapping of particles in three-dimensional structures through time-sharing. This method allows particles to be held in complex, three-dimensional configurations using cycling of simple holograms. Importantly, we discuss limiting factors inherent in current phase only SLM design for applications in both optical tweezing and atom trapping.
Publisher: Optica Publishing Group
Date: 20-10-2021
Abstract: Ghost imaging captures 2D images with a point detector instead of an array sensor. It could therefore solve the challenge of building cameras in wave bands where sensors are difficult and expensive to produce and could open up more routine THz, near-infrared, lifetime, and hyperspectral imaging simply by using single-pixel detectors. Traditionally, ghost imaging retrieves the image of an object offline by correlating measured light intensities with pre-designed illuminating patterns. Here we present a “self-evolving” ghost imaging (SEGI) strategy for imaging objects bypassing offline post-processing. It also offers the capability to image objects in turbid media. By inspecting the optical feedback, we evaluate the illumination patterns by a cost function and generate offspring illumination patterns that mimic the object’s image, bypassing the reconstruction process. At the initial evolving state, the object’s “genetic information” is stored in the patterns. At the following imaging stage, the object’s image ( 48 × 48 p i x e l s ) can be updated at a 40 Hz imaging rate. We numerically and experimentally demonstrate this concept for static and moving objects. The frame-memory effect between the self-evolving illumination patterns provided by the genetic algorithm enables SEGI imaging through turbid media. We further demonstrate this capability by imaging an object placed in a container filled with water and sand. SEGI shows robust and superior imaging power compared with traditional computational ghost imaging. This strategy could enhance ghost imaging in applications such as remote sensing, imaging through scattering media, and low-irradiative biological imaging.
Publisher: Springer Science and Business Media LLC
Date: 09-2002
DOI: 10.1038/NATURE01007
Publisher: IOP Publishing
Date: 04-2023
Abstract: Optical tweezers are tools made of light that enable contactless pushing, trapping, and manipulation of objects, ranging from atoms to space light sails. Since the pioneering work by Arthur Ashkin in the 1970s, optical tweezers have evolved into sophisticated instruments and have been employed in a broad range of applications in the life sciences, physics, and engineering. These include accurate force and torque measurement at the femtonewton level, microrheology of complex fluids, single micro- and nano-particle spectroscopy, single-cell analysis, and statistical-physics experiments. This roadmap provides insights into current investigations involving optical forces and optical tweezers from their theoretical foundations to designs and setups. It also offers perspectives for applications to a wide range of research fields, from biophysics to space exploration.
Publisher: The Optical Society
Date: 11-11-2011
Publisher: IOP Publishing
Date: 31-07-2009
Publisher: Frontiers Media SA
Date: 26-01-2021
DOI: 10.3389/FIMMU.2021.792813
Abstract: Integrins in effector T cells are crucial for cell adhesion and play a central role in cell-mediated immunity. Leukocyte adhesion deficiency (LAD) type III, a genetic condition that can cause death in early childhood, highlights the importance of integrin/kindlin interactions for immune system function. A TTT/AAA mutation in the cytoplasmic domain of the β 2 integrin significantly reduces kindlin-3 binding to the β 2 tail, abolishes leukocyte adhesion to intercellular adhesion molecule 1 (ICAM-1), and decreases T cell trafficking in vivo . However, how kindlin-3 affects integrin function in T cells remains incompletely understood. We present an examination of LFA-1/ICAM-1 bonds in both wild-type effector T cells and those with a kindlin-3 binding site mutation. Adhesion assays show that effector T cells carrying the kindlin-3 binding site mutation display significantly reduced adhesion to the integrin ligand ICAM-1. Using optical trapping, combined with back focal plane interferometry, we measured a bond rupture force of 17.85 ±0.63 pN at a force loading rate of 30.21 ± 4.35 pN/s, for single integrins expressed on wild-type cells. Interestingly, a significant drop in rupture force of bonds was found for TTT/AAA-mutant cells, with a measured rupture force of 10.08 ± 0.88pN at the same pulling rate. Therefore, kindlin-3 binding to the cytoplasmic tail of the β 2-tail directly affects catch bond formation and bond strength of integrin–ligand bonds. As a consequence of this reduced binding, CD8+ T cell activation in vitro is also significantly reduced.
Publisher: Elsevier BV
Date: 02-2016
Publisher: SPIE
Date: 07-02-2018
DOI: 10.1117/12.2288485
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 07-2021
Publisher: SPIE
Date: 09-10-2012
DOI: 10.1117/12.930126
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 10-2021
Publisher: Optica Publishing Group
Date: 03-2010
Publisher: IOP Publishing
Date: 12-06-2009
Publisher: Elsevier BV
Date: 05-2013
Publisher: AIP Publishing
Date: 21-07-2008
DOI: 10.1063/1.2952374
Abstract: We demonstrate that holographically generated optical patterns offer greater flexibility for the thermocapillary control of water droplets than Gaussian spots droplets can be stopped in faster flows while using less optical intensity when the surface tension variations are created by line patterns instead of single spots. Further, experiments are performed making use of variable light patterns to achieve controlled droplet routing in a four-way cross microfluidic channel. Finally, multiple droplet storage is demonstrated as well as changing drop order.
Publisher: AIP Publishing
Date: 21-04-2008
DOI: 10.1063/1.2912031
Abstract: This paper describes the quantitative force mapping of micron-sized particles held in an optical vortex trap. We present a simple and efficient model, which accounts for the diffraction of the strongly localized optical field of the tightly focused laser beam, the spherical aberration introduced by the dielectric glass-to-water interface, employs the multidipole approximation for force calculations, and is able to reproduce, with quantitative agreement, the experimentally measured force map.
Publisher: IOP Publishing
Date: 26-10-2009
Publisher: AIP Publishing
Date: 09-01-2012
DOI: 10.1063/1.3676414
Abstract: We demonstrate directed jetting from pulsed laser-induced cavities subjected to a burst of focused ultrasound. Alignment of the ultrasound focus and the pressure litudes in the vicinity of the cavity dictate the direction and length of the resulting jet, respectively. We interpret our observations in terms of radiation forces exerted on the cavity, due to the pressure gradient introduced to the ultrasound focus by its presence. We support our hypothesis with a linear analysis of the force distribution across the cavity surface, at the moment of maximum inflation, which shows reasonable predictive agreement with the observed jet characteristics.
Publisher: IEEE
Date: 2000
Publisher: Optica Publishing Group
Date: 2020
DOI: 10.1364/CLEOPR.2020.C11B_3
Abstract: A terahertz (THz) reflect-array is proposed. Dual circularly polarized (left- and right-hand-circular-polarizations) collimated beams are independently manipulated. In our model, the left-hand-circularly-polarized and right-hand-circularly-polarized beams reflect at 23-degrees along the y-direction and x-direction respectively.
Publisher: Cold Spring Harbor Laboratory
Date: 19-12-2018
DOI: 10.1101/501775
Abstract: Oriented cell intercalations and cell shape changes are key determinants of large-scale epithelial cell sheet deformations occurring during gastrulation in many organisms. In several cases directional intercalation and cell shape changes have been shown to be associated with a planar cell polarity in the organisation of the actin-myosin cytoskeleton of epithelial cells. This polarised cytoskeletal organisation has been postulated to reflect the directional tension necessary to drive and orient directional cell intercalations. We have now further characterised and applied a recently introduced non-destructive optical manipulation technique to measure the tension in in idual cell junctions in the epiblast of chick embryos in the early stages of primitive streak formation. We have measured junctional tension as a function of position and orientation. Junctional tension of mesendoderm cells, the tissue that drives the formation of the streak, is higher than tension of junctions of cells in other parts of the epiblast. Furthermore, in the mesendoderm junctional tension is higher in the direction of intercalation. The data are fitted best with a Maxwell model and we find that both junctional tension and relaxation time are dependent on myosin activity.
Publisher: Informa UK Limited
Date: 07-2003
Publisher: The Optical Society
Date: 11-08-2011
DOI: 10.1364/OE.19.016432
Publisher: OSA
Date: 2009
Publisher: Springer Science and Business Media LLC
Date: 02-2014
DOI: 10.1038/506437A
Abstract: Might it be possible to create mirrors for space telescopes, using nothing but microscopic particles held in place by light? A study that exploits a technique called optical binding provides a step towards this goal.
Publisher: SPIE
Date: 30-04-2010
DOI: 10.1117/12.854008
Publisher: SPIE
Date: 28-08-2008
DOI: 10.1117/12.793726
Publisher: OSA
Date: 2013
Publisher: American Chemical Society (ACS)
Date: 12-2006
DOI: 10.1021/AC061586W
Publisher: American Physical Society (APS)
Date: 15-08-2007
Publisher: SPIE
Date: 14-03-2005
DOI: 10.1117/12.606336
Publisher: Optica Publishing Group
Date: 27-01-2003
DOI: 10.1364/OE.11.000158
Abstract: We discuss the application of spatial light modulators (SLMs) to the field of atom optics. We show that SLMs may be used to generate a wide variety of optical potentials that are useful for the guiding and dipole trapping of atoms. This functionality is demonstrated by the production of a number of different light potentials using a single SLM device. These include Mach-Zender interferometer patterns and the generation of a bottle-beam. We also discuss the current limitations in SLM technology with regard to the generation of both static and dynamically deformed potentials and their use in atom optics.
Publisher: American Physical Society (APS)
Date: 29-08-2003
Publisher: SPIE
Date: 09-10-2012
DOI: 10.1117/12.948748
Publisher: Optica Publishing Group
Date: 20-01-1998
DOI: 10.1364/AO.37.000469
Abstract: A stressed fiber-optic waveguide is used to impart orbital angular momentum to a Hermite-Gaussian (HG(10)) laser mode. The transmitted beam has an annular intensity profile and a well-defined azimuthal phase dependence. We confirm the phase structure of the beam by observing the interference pattern produced between it and a plane wave. The transfer of the angular momentum to the light occurs because of a difference in phase velocity within the fiber for two orthogonal modes that comprise the input beam. This represents a mechanism for the transfer of orbital angular momentum to a light beam that has not hitherto been identified.
Publisher: IOP Publishing
Date: 27-02-2013
Publisher: IOP Publishing
Date: 23-06-2003
Publisher: Wiley
Date: 07-10-2017
Abstract: Bladder cancer is among the most common cancers in the UK and conventional detection techniques suffer from low sensitivity, low specificity, or both. Recent attempts to address the disparity have led to progress in the field of autofluorescence as a means to diagnose the disease with high efficiency, however there is still a lot not known about autofluorescence profiles in the disease. The multi-functional diagnostic system "LAKK-M" was used to assess autofluorescence profiles of healthy and cancerous bladder tissue to identify novel biomarkers of the disease. Statistically significant differences were observed in the optical redox ratio (a measure of tissue metabolic activity), the litude of endogenous porphyrins and the NADH orphyrin ratio between tissue types. These findings could advance understanding of bladder cancer and aid in the development of new techniques for detection and surveillance.
Publisher: Optica Publishing Group
Date: 05-2006
DOI: 10.1364/OE.14.004176
Abstract: We demonstrate the use of holographic optical tweezers for trapping particles in air, specifically aerosol droplets. We show the trapping and manipulation of arrays of liquid aerosols as well as the controlled coagulation of two or more droplets. We discuss the ability of spatial light modulators to manipulate airborne droplets in real time as well as highlight the difficulties associated with loading and trapping particles in such an environment. We conclude with a discussion of some of the applications of such a technique.
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 06-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7CP01468F
Abstract: Temperature-dependent Raman spectra of tetradecane, pentadecane and hexadecane are collected and analysed to unveil the difference in the speed of their phase transitions while gaining information about their structural changes.
Publisher: IEEE
Date: 07-2013
Publisher: Optica Publishing Group
Date: 09-10-2007
DOI: 10.1364/OE.15.013972
Abstract: Spatially periodic optical fields can be used to sort dielectric microscopic particles as a function of size, shape or refractive index. In this paper we elucidate through both theory and experiment the behavior of silica microspheres moving under the influence of the periodic optical field provided by a Bessel beam. We compare two different computational models, one based on Mie scattering, the other on geometrical ray optics and find good qualitative agreement, with both models predicting the existence of distinct size-dependent phases of particle behavior. We verify these predictions by providing experimental observations of the in idual behavioral phases.
Publisher: OSA
Date: 2006
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
Start Date: 2022
End Date: 06-2023
Amount: $520,000.00
Funder: Australian Research Council
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