ORCID Profile
0000-0002-2056-4802
Current Organisations
Hiroshima University
,
State Health System Resoiurce Center
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Publisher: Elsevier BV
Date: 06-2005
DOI: 10.1016/J.MOLIMM.2004.11.010
Abstract: We previously demonstrated the immunosuppressive activity of anti-histone H1 autoreactive antibodies (Ab) transiently induced in serum of a rat tolerogenic orthotopic liver transplantation (OLT) model. In the present study, we investigated the effects of anti-histone H1 Ab on dendritic cells (DCs), T-cells, lymphokine-activated killer (LAK) cells, and human natural killer (NK) cells. The effects of anti-histone H1 Ab on Concanavalin A (ConA) blast, on rat DC cytokine profiles and phenotypes, and on T-cells, LAK cells, and human NK cells were examined by flow cytometry and RT-PCR. The cytotoxicity of LAK and NK cells pretreated with anti-histone H1 Ab was assayed. The addition of anti-histone H1 Ab to ConA blast inhibited the proliferation of 5-(6)-carboxy-fluorescein succinimidyl ester (CFSE)-labeled lymphocytes without toxicity but increased the population of CD4+CD25+ T-cells. DCs treated with anti-histone H1 Ab expressed lower levels of CD80/CD86, IL-1beta, and IL-6. The addition of anti-histone H1 Ab to LAK culture decreased the percentages of NKR-P1 populations and down-regulated levels of inducible nitric oxide synthase (iNOS), IL-2, and INF-gamma in RT-PCR. The cytotoxicity of LAK and NK cells was lower when pretreated with anti-histone H1 Ab than when pretreated with control IgG. We found that the blockade of histone H1 modulated DCs toward tolerogenic status, decreased the cytotoxicity of LAK and NK cells, and induced CD4+CD25+ T-cells. These results suggest that the use of anti-histone H1 Abs might be a useful strategy for the development of a form of immunosuppression.
Publisher: JMIR Publications Inc.
Date: 05-01-2018
Abstract: nadequate administrative health data, sub-optimal public health infrastructure, rapid and unplanned urbanization,environmental degradation and poor penetration of information technology make the tracking of health and well being of the populations within developing countries more challenging. This necessitates setting-up comprehensive surveillance platforms integrated with the information technologies that can cater to the full spectrum of the public health problems. his manuscript aims to provide methodological insights on establishing GIS integrated comprehensive surveillance platform in resource constrained rural settings. he INCLEN (International Clinical Epidemiology Network) Trust International established a comprehensive SOMAARTH Demographic, Development and Environmental Surveillance Site (DDESS) in a northern Indian rural setting. The surveillance platform evolved through adopting four major steps: 1) site preparation 2) data construction 3) data quality assurance 4) data update and maintenance system. Arc GIS 10.3 and QGIS 2.14 software were employed for geo-spatial data construction. Surveillance data architecture was built upon the geo-referenced land parcel data sets. The composition data pertaining to the land use (residential, non-residential, and vacant), water bodies, roads, railways, community trails, landmarks, water, sanitation and food environment, weather and air quality, demographic characteristics were constructed in relational manner within the surveillance platform. comprehensive surveillance platform encompassing 0.2 million population residing in 51 villages over a land mass of 251.7 sq. Km having 32,662 households and 19,260 nonresidential features (cattle shed, shops, health, education, banking, religious institutions etc.) is established. The processes adopted for sub ision of villages into sectors helped in developing geo-referenced location identification system in a setting where no postal addresses or postal codes system were in place. Also the socially and economically homogeneous community clusters (78% of 676 sectors) which usually hide within the village aggregates were disclosed. Characterization and storage of variety of data sets critical for health and epidemiology and generation of new information e.g. water, sanitation and hygiene through geo-analytics were demonstrated. Settlement pattern was compact to the extent that 80% of habitation was concentrated in 9% of the total village area. Community involvement proved helpful in the ground-truthing of the data sets for ascertaining the level of positional, temporal and attribute accuracies and identification of small habitations, missing in the official records. OMAARTH experience allowed characterization and monitoring of wide range of attributes from demography, development, and environmental domains and developed geospatial inter-phase to explore and explain their dynamic relationships, associations and pathways across multiple levels i.e. in idual, household, neighborhood, and village. The methodology takes care of the common challenges faced while building information system in the developing countries. However generalizability and scalability needs to be tested in other resource constrained settings as well.
Publisher: JMIR Publications Inc.
Date: 05-10-2018
Publisher: Elsevier BV
Date: 02-2001
Location: India
No related grants have been discovered for Seiji Kawamoto.