ORCID Profile
0000-0003-0762-5727
Current Organisation
Department of Primary Industries and Regional Development
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Research Topics
In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Top 5 Research Topics
ANZSRC Field of Research (FoR)
Crop and pasture production | Animal reproduction and breeding | Horticultural crop improvement (incl. selection and breeding) | Crop and pasture improvement (incl. selection and breeding)
ANZSRC Socio-Economic Objective (SEO)
Related Links
Publications
Genomic Regions, Molecular Markers, and Flanking Genes of Metribuzin Tolerance in Wheat (Triticum aestivum L.)
Publisher: Frontiers Media SA
Date: 19-05-2022
Abstract: Understanding the genetics of metribuzin (a group C herbicide) tolerance in wheat is vital in developing tolerant cultivars to improve wheat productivity in dryland farming systems. This study investigated metribuzin tolerance in wheat by conducting a Genome-wide Association Studies (GWAS) with a panel of 150 wheat genotypes of erse genetic backgrounds and genotyped them with the wheat 90 K SNP genotyping assay. The phenotyping was conducted in a temperature-controlled glasshouse at the University of Western Australia (UWA). Genotypes were sprayed with a metribuzin dose of 400 grams of active ingredient (g. a.i.) ha −1 as pre-emergent in a specialized spraying cabinet and transferred to the glasshouse where the tolerance level of the genotypes was assessed by measuring the relative reduction in chlorophyll content of the leaves. The decrease in chlorophyll content of the treated plants compared to the control was regarded as the phytotoxic effects of metribuzin. GWAS analysis following a mixed linear model revealed 19 genomic regions with significant marker-trait associations (MTAs), including ten on chromosome 6A, three on chromosome 2B, and one on chromosomes 3A, 5B, 6B 6D, 7A, and 7B, respectively. Sequences of the significant markers were blasted against the wheat genome, IWGSC RefSeq V1.0, and candidate genes having annotations related to herbicide tolerance in wheat, especially in pathways reported to be involved in metribuzin tolerance, such as cytochrome P450 pathways and ATP Binding Cassette (ABC) superfamilies, were identified in these genomic regions. These included TraesCS6A01G028800, TraesCS6A02G353700, TraesCS6A01G326200, TraesCS7A02G331000, and TraesCS2B01G465200. These genomic regions were validated on 30 top tolerant and 30 most susceptible genotypes using the five closest SSR makers to the flanked SNPs. Sufficient polymorphism was detected on two markers ( wms193 and barc1036 ) that were found to differentiate between the susceptible and tolerant alleles and a t-test analysis of the phenotypic data shows a significant (value of p & 0.001) difference suggesting that these markers can be used for marker-assisted selection (MAS) in metribuzin studies and wheat breeding programs.
Development of near-isogenic lines targeting a major QTL on 3AL for pre-harvest sprouting resistance in bread wheat
Publisher: CSIRO Publishing
Date: 2018
DOI: 10.1071/CP17423
Abstract: Resistance to pre-harvest sprouting (PHS) in wheat (Triticum aestivum L.) is one of the most valuable traits in many breeding programs. However, the quantitative nature of inheritance of PHS resistance challenges the study of this trait. Near-isogenic lines (NILs) can turn a complicated quantitative trait into a Mendelian factor (qualitative) and are, therefore, valuable materials for identification of the gene(s) responsible for a specific phenotypic trait and for functional studies of specific loci. Five pairs of NILs were developed and confirmed for a major quantitative trait locus (QTL) located on the long arm of chromosome 3A contributing to PHS resistance in wheat. These NILs were generated by using the heterogeneous inbred family method and a fast generation-cycling system. Significant differences in PHS resistance between the isolines were detected in the NILs. The presence of the PHS-resistance allele from the resistant parent increased resistance to sprouting on spikes by 26.7–96.8%, with an average of 73.8%, and increased seed dormancy by 36.9–87.2%, with an average of 59.9% across the NILs. These NILs are being used for the identification of candidate genes responsible for this major PHS-resistance locus on wheat chromosome arm 3AL.
Proteomic analysis of near-isogenic lines reveals key biomarkers on wheat chromosome 4B conferring drought tolerance
Publisher: Wiley
Date: 18-05-2023
DOI: 10.1002/TPG2.20343
Abstract: Drought is a major constraint for wheat production that is receiving increased attention due to global climate change. This study conducted isobaric tags for relative and absolute quantitation proteomic analysis on near‐isogenic lines to shed light on the underlying mechanism of qDSI.4B.1 quantitative trait loci (QTL) on the short arm of chromosome 4B conferring drought tolerance in wheat. Comparing tolerant with susceptible isolines, 41 differentially expressed proteins were identified to be responsible for drought tolerance with a p ‐value of 0.05 and fold change .3 or .7. These proteins were mainly enriched in hydrogen peroxide metabolic activity, reactive oxygen species metabolic activity, photosynthetic activity, intracellular protein transport, cellular macromolecule localization, and response to oxidative stress. Prediction of protein interactions and pathways analysis revealed the interaction between transcription, translation, protein export, photosynthesis, and carbohydrate metabolism as the most important pathways responsible for drought tolerance. The five proteins, including 30S ribosomal protein S15, SRP54 domain‐containing protein, auxin‐repressed protein, serine hydroxymethyltransferase, and an uncharacterized protein with encoding genes on 4BS, were suggested as candidate proteins responsible for drought tolerance in qDSI.4B.1 QTL. The gene coding SRP54 protein was also one of the differentially expressed genes in our previous transcriptomic study.
Transcriptome Analyses of Near Isogenic Lines Reveal Putative Drought Tolerance Controlling Genes in Wheat
Publisher: Frontiers Media SA
Date: 29-03-2022
Abstract: The workflow of this study. (1) Near isogenic lines for qDSI.4B.1 QTL responsible for drought tolerance were grown in control and drought stress conditions, (2) grain s les were collected at 7 and 14 days after stress initiation at anthesis, (3) RNA was extracted, (4) sequenced and (5) data were analysed and organized with focus on 4BS, (6) six candidate genes were found for drought tolerance in qDSI.4B.1 interval and sequencing results were confirmed by qRT-PCR, (7) the protein products and (8) molecular function of the candidate genes were future studied.
Identification of KASP markers and candidate genes for drought tolerance in wheat using 90K SNP array genotyping of near-isogenic lines targeting a 4BS quantitative trait locus
Publisher: Springer Science and Business Media LLC
Date: 16-08-2023
DOI: 10.1007/S00122-023-04438-3
Abstract: This study identified a novel SNP and developed a highly efficient KASP marker for drought tolerance in wheat by genotyping NILs targeting a major QTL for drought tolerance using an SNP array and validation with commercial varieties. Common wheat ( Triticum aestivum L.) is an important winter crop worldwide and a typical allopolyploid with a large and complex genome. With global warming, the environmental volatility and incidence of drought in wheat-producing areas will increase. Molecular markers for drought tolerance are urgently needed to enhance drought tolerance breeding. Here, we genotyped four near-isogenic line (NIL) pairs targeting a major QTL qDSI.4B.1 on wheat chromosome arm 4BS for drought tolerance using the 90K SNP Illumina iSelect array and discovered a single nucleotide polymorphism (SNP) (Excalibur_c100336_106) with consistent genotype–phenotype associations among all four NIL pairs and their parents. Then, we converted the SNP into a Kompetitive Allele-Specific PCR (KASP) marker, with an accuracy of 100% for the four NIL pairs and their parents and as high as 81.8% for the 44 tested wheat lines with known phenotypes collected from Australia and China. Two genes near this SNP were suggested as candidate genes for drought tolerance in wheat after checking the Chinese Spring reference genome annotation version 1.1. One gene, TraesCS4B02G085300, encodes an F-box protein reportedly related to the ABA network, a main pathway for drought tolerance, and another gene, TraesCS4B02G085400, encodes a calcineurin-like metallophos-phoesterase transmembrane protein, which participates in Ca 2+ -dependent phosphorylation regulatory system. Based on this work and previous research on pre-harvest sprouting, we established a quick and efficient general SQV-based approach for KASP marker development, integrating genotyping by SNP arrays (S) using NILs targeting major QTL for a specific trait (Q) and validating them with commercial varieties (V). The identified SNP and developed KASP marker could be applied to marker-assisted selection in drought breeding, and further study of the candidate genes may improve our understanding of drought tolerance in wheat.
Response of wheat to post-anthesis water stress, and the nature of gene action as revealed by combining ability analysis
Publisher: CSIRO Publishing
Date: 2017
DOI: 10.1071/CP17112
Abstract: Post-anthesis water stress is a major limitation to wheat grain yield globally. Understanding the nature of gene action of yield related traits under post-anthesis water stress will help to breed stress-resilient genotypes. Four bread wheat genotypes having varying degree of drought tolerance were crossed in a full-diallel fashion and the resultant crosses along with the parental genotypes, were subjected to water stress after the onset of anthesis in order to investigate their comparative performance and nature of gene action. Parental genotypes Babax (B) and Westonia (W) performed better compared with C306 (C) and Dharwar Dry (D) with respect to relative reduction in grain yield and related traits under stressed condition. Direct cross B × D and reciprocal cross W × C were more tolerant to water stress, while cross between C306 and Dharwar Dry, either direct or reciprocal, produced more sensitive genotypes. Combining ability analysis revealed that both additive and non-additive gene action were involved in governing the inheritance of the studied traits, with predominance of non-additive gene action for most of the traits. Among the parents, Babax and Westonia were better combiners for grain yield under stress condition. B × D in stressed condition, and C × W in both stressed and stress-free conditions, were the most suitable specific crosses. Moreover, specificity of parental genotypes as female parents in cross combination was also evident from the significant reciprocal combining ability effects of certain traits. Low to medium narrow sense heritability and high broad sense heritability were observed for most of the studied traits in both well watered and water stress conditions. The results of the study suggested that specific cross combinations with high specific combining ability involving better performing parents with high general combining ability may generate hybrids as well as segregating populations suitable for further breeding programs.
Phenotypic and genotypic characterization of near-isogenic lines targeting a major 4BL QTL responsible for pre-harvest sprouting in wheat
Publisher: Springer Science and Business Media LLC
Date: 09-08-2019
Genetic divergence of indigenous pummelo genotypes
Publisher: Bangladesh Journals Online (JOL)
Date: 26-05-2016
Abstract: The genetic ergence was studied in33pummelogenotypes using D2 statistics and principal component analysis at Regional Agricultural Research Station, BARI, Akbarpur, Moulvibazar during 2012 to 2014. The genotypes were grouped into 5 clusters and the maximum number of genotypes was included in cluster IV and V and the minimum number in cluster I. The inter cluster distance in all of the cases were higher than the intra cluster distance indicating wider genetic ersity among the accessions of different groups. The highest inter-cluster distance was observed between cluster I and II followed by cluster II and V and the lowest between III and IV. The highest intra- cluster distance was observed for the cluster II and the lowest for the cluster III. For cluster II, the highest mean values for plant height (6.13m), in idual fruit weight (1141.67g), fruit length (13.03 cm) and breadth (13.15 cm), number of segments per fruit (14.41), number and weight of seeds per fruit (123.67 and 50.41g), yield per plant (50.94 kg) were observed. The first axis largely accounted for the variation among the pummelo accessions (26.16%) followed by second axis (18.75%). The first 8 axes accounted 90.56 % of the total variation. The characters in idual fruit weight (g) and weight of seeds per fruit (g) showing positive value in both the vectors contributed maximum towards ergence. Considering magnitude of genetic distance, contribution of different traits toward the total ergence, magnitude of cluster means for different traits and performance the genotypes of cluster I, II, and IV may be considered as parents for future hybridization program.Res. Agric., Livest. Fish.3(1): 37-43, April 2016
Multiple near-isogenic lines targeting a QTL hotspot of drought tolerance showed contrasting performance under post-anthesis water stress
Publisher: Frontiers Media SA
Date: 08-03-2019
Root transcriptome profiling of contrasting wheat genotypes provides an insight to their adaptive strategies to water deficit
Publisher: Springer Science and Business Media LLC
Date: 17-03-2020
DOI: 10.1038/S41598-020-61680-1
Abstract: Water deficit limits plant growth and productivity in wheat. The effect of water deficit varies considerably in the contrasting genotypes. This study attempted comparative transcriptome profiling of the tolerant (Abura) and susceptible (AUS12671) genotypes under PEG-simulated water stress via genome-wide RNA-seq technology to understand the dynamics of tolerance mechanism. Morphological and physiological analyses indicated that the tolerant genotype Abura had a higher root growth and net photosynthesis, which accounted for its higher root biomass than AUS12671 under stress. Transcriptomic analysis revealed a total of 924 differentially expressed genes (DEGs) that were unique in the contrasting genotypes under stress across time points. The susceptible genotype AUS12671 had slightly more abundant DEGs (505) than the tolerant genotype Abura (419). Gene ontology enrichment and pathway analyses of these DEGs suggested that the two genotypes differed significantly in terms of adaptive mechanism. Predominant upregulation of genes involved in various metabolic pathways was the key adaptive feature of the susceptive genotype AUS12671 indicating its energy-consuming approach in adaptation to water deficit. In contrast, downregulation the expression of genes of key pathways, such as global and overview maps, carbohydrate metabolism, and genetic information processing was the main strategy for the tolerant genotype Abura. Besides, significantly higher number of genes encoding transcription factors (TF) families like MYB and NAC, which were reported to be associated with stress defense, were differentially expressed in the tolerant genotype Abura. Gene encoding transcription factors TIFY were only differentially expressed between stressed and non-stressed conditions in the sensitive genotype. The identified DEGs and the suggested differential adaptive strategies of the contrasting genotypes provided an insight for improving water deficit tolerance in wheat.
Related Organisations
Department Of Primary Industries And Regional Development
Location: Australia
Related Funding Activities
Industrial Transformation Training Centres - Grant ID: IC230100016
Start Date: 2023
End Date: 12-2027
Amount: $5,000,000.00
Funder: Australian Research Council
View Funded Activity