ORCID Profile
0000-0003-2326-7595
Current Organisation
University of Leeds
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Publisher: Springer Science and Business Media LLC
Date: 08-03-2006
Abstract: The identification of protein trafficking signals, and their interacting mechanisms, is a fundamental objective of modern biology. Unfortunately, the analysis of trafficking signals is complicated by their topography, hierarchical nature and regulation. Powerful strategies to test candidate motifs include their ability to direct simpler reporter proteins, to which they are fused, to the appropriate cellular compartment. However, present reporters are limited by their endogenous expression, paucity of cloning sites, and difficult detection in live cells. Consequently, we have engineered a mammalian expression vector encoding a novel trafficking reporter – pIN-G – consisting of a simple, type I integral protein bearing permissive intra/extracellular cloning sites, green fluorescent protein (GFP), cMyc and HA epitope tags. Fluorescence imaging, flow cytometry and biochemical assays of transfected HEK293 cells, confirm the size, topology and surface expression of PIN-G. Moreover, a pIN-G fusion construct, containing a Trans-Golgi Network (TGN) targeting determinant, internalises rapidly from the cell surface and localises to the TGN. Additionally, another PIN-G fusion protein and its mutants reveal trafficking determinants in the cytoplasmic carboxy terminus of Kv1.4 voltage-gated potassium channels. Together, these data indicate that pIN-G is a versatile, powerful, new reporter for analysing signals controlling membrane protein trafficking, surface expression and dynamics.
Publisher: American Psychological Association (APA)
Date: 04-2017
DOI: 10.1037/COU0000193
Abstract: We tested a model based on goal-setting and self-regulation theories of the cross-lagged relationships among negative career-related feedback, negative affect (career-related stress), and career goal revision (downward goal revision and goal disengagement). Participants were 409 Chinese university/college students (Mage 19 years 58% female), who completed a survey at 2 time points approximately 6 months apart. Consistent with our hypotheses, negative career-related feedback at T1 was related to more career goal disengagement and greater downward goal revision at T2. Career-related stress partially mediated the relationship between negative career-related feedback and downward goal revision. In addition, there were reverse relationships between negative career-related feedback and career-related stress, and between career-related stress and goal disengagement. These findings highlight important roles for negative career-related feedback and negative affect in young peoples' career goal pursuit. (PsycINFO Database Record
Publisher: SAGE Publications
Date: 21-09-2016
Abstract: Based on goal-setting theory, this study examined the relationship between negative career goal feedback and career-related stress, tested whether career goal–performance discrepancy operated as a mediator in this relationship, and assessed whether career goal importance strengthened the indirect effect of negative feedback on stress via discrepancy. Using a s le of 317 health profession university students (mean age = 19.5 years), we found that negative feedback was associated positively with stress and that discrepancy mediated this relationship. Consistent with goal-setting theory, we also found that discrepancy was higher at higher levels of negative feedback for those with higher goal importance, and the indirect effect of negative feedback on stress through discrepancy increased with increasing goal importance. These findings highlight important roles for career goal feedback and career goal importance in young peoples’ career goal pursuit.
Publisher: Elsevier BV
Date: 04-2017
Publisher: Elsevier BV
Date: 02-2020
Publisher: Elsevier BV
Date: 10-2017
Publisher: SAGE Publications
Date: 09-01-2020
Abstract: Informed by goal-setting/self-regulatory theories, we tested the mediating role of career-related effort (i.e., goal striving) in the relationships between career-related indecision (i.e., goal ambiguity) and career-related stress (i.e., affect) and perceived employability (i.e., career-related attitude) and examined the effect of financial distress as a moderator in these direct and indirect relationships. Using a s le of 202 young adults ( M age = 19.8 years, 81.7% female), we found career indecision was related negatively to effort and perceived employability and positively to stress, with effort mediating between indecision and both stress and perceived employability. However, financial distress influenced these relationships. The associations between career indecision and effort and perceived employability were more negative and the associations between career indecision and stress were more positive when financial distress was higher. The study contributes by identifying how financial distress affects the relationships between career indecision, effort, and other career variables.
Publisher: Elsevier BV
Date: 06-2018
Publisher: SAGE Publications
Date: 19-06-2016
Abstract: Career goal feedback provides information about career goal suitability, adequacy of goal progress, and whether changes are needed to reach the goals. Feedback comes from external (e.g., parents, peers) and internal sources (e.g., self-reflection), and plays an important role in the career development of young people. As there is no existing measure that adequately captures this construct, we devised and validated a 24-item inventory for use with young adults. In Study 1, initial items were developed, expert reviewed, and administered to a s le of Chinese university students ( N = 1,055 M AGE = 19 years). We used exploratory factor analysis to test the factor structure and confirmatory factor analysis on a holdout s le to validate a third-order solution (one third-order factor manifested by three second-order factors). In addition, we provided evidence for convergent and incremental validity. In Study 2, we confirmed the factor structure on Australian university students ( N = 184 M AGE = 19 years).
Publisher: Ovid Technologies (Wolters Kluwer Health)
Date: 14-05-2010
DOI: 10.1161/CIRCRESAHA.110.219329
Abstract: Rationale : Transient receptor potential melastatin (TRPM)3 is a calcium-permeable ion channel activated by the neurosteroid pregnenolone sulfate and positively coupled to insulin secretion in β cells. Although vascular TRPM3 mRNA has been reported, there is no knowledge of TRPM3 protein or its regulation and function in the cardiovascular system. Objective : To determine the relevance and regulation of TRPM3 in vascular biology. Methods and Results : TRPM3 expression was detected at mRNA and protein levels in contractile and proliferating vascular smooth muscle cells. Calcium entry evoked by pregnenolone sulfate or sphingosine was suppressed by TRPM3 blocking antibody or knock-down of TRPM3 by RNA interference. Low-level constitutive TRPM3 activity was also detected. In proliferating cells, channel activity was coupled negatively to interleukin-6 secretion via a calcium-dependent mechanism. In freshly isolated aorta, TRPM3 positively modulated contractile responses independently of L-type calcium channels. Concentrations of pregnenolone sulfate required to evoke responses were higher than the known plasma concentrations of the steroids, leading to a screen for other stimulators. β-Cyclodextrin was one of few stimulators of TRPM3, revealing the channels to be partially suppressed by endogenous cholesterol, the precursor of pregnenolone. Elevation of cholesterol further suppressed channel activity and loading with cholesterol to generate foam cells precluded observation of TRPM3 activity. Conclusions : The data suggest functional relevance of TRPM3 in contractile and proliferating phenotypes of vascular smooth muscle cells, significance of constitutive channel activity, regulation by cholesterol, and potential value of pregnenolone sulfate in therapeutic vascular modulation.
Publisher: American Psychological Association (APA)
Date: 11-2018
DOI: 10.1037/COU0000282
Abstract: Based on social-cognitive and general self-regulation theories, this study examined the underlying mechanisms of the within-person relationship between negative career goal feedback and career-related stress. Using a s le of young adults and a weekly survey study with four measurements (212 observations), we found that negative feedback on career progress and poor feedback on improvements needed for career progress related to more career stress, and that career-related self-efficacy mediated the link between feedback on improvements needed and stress. In addition, trait optimism buffered the link between negative feedback on progress and stress. These findings highlight important roles for career feedback and optimism in young people's career self-regulation. (PsycINFO Database Record (c) 2018 APA, all rights reserved).
Publisher: Springer Science and Business Media LLC
Date: 16-08-2019
Publisher: SAGE Publications
Date: 15-09-2020
Abstract: Based on the life history perspective, this study tested a serial mediation model in which family socioeconomic status (SES) related to person–job fit via resource scarcity, career exploration, and goal persistence. We expected that when seeking employment, higher SES students would perceive lower resource scarcity, and, in turn, adopt more adaptive career behaviors (i.e., career exploration and goal persistence) to maximize career success, which would then lead to better person–job fit upon graduation. Using a s le of 224 final-year students (mean age 21 years, 77% male), we found, as expected, that higher SES was related to more career exploration and goal persistence via lower perceived scarcity and that exploration and persistence were related to better person–job fit. In addition, higher SES was indirectly related to person–job fit via scarcity and the two career behaviors. The findings highlight the importance of family SES in young people’s career development.
Publisher: Elsevier BV
Date: 02-2019
Publisher: Informa UK Limited
Date: 17-11-2022
Location: United Kingdom of Great Britain and Northern Ireland
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