ORCID Profile
0000-0001-9827-9836
Current Organisations
GlaxoSmithKline (United Kingdom)
,
University of Oxford
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Research Topics
In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Top 5 Research Topics
ANZSRC Field of Research (FoR)
Resources Engineering and Extractive Metallurgy | Petroleum and Reservoir Engineering | Functional Materials
ANZSRC Socio-Economic Objective (SEO)
Oil and Gas Exploration | Oil and Gas Extraction | Expanding Knowledge in Engineering |
Related Links
Publications
Direct measurement of hydrogen relative permeability hysteresis for underground hydrogen storage
Publisher: Elsevier BV
Date: 08-2023
Deep Immune Phenotyping and Single-Cell Transcriptomics Allow Identification of Circulating TRM-Like Cells Which Correlate With Liver-Stage Immunity and Vaccine-Induced Protection From Malaria
Publisher: Frontiers Media SA
Date: 07-02-2022
DOI: 10.3389/FIMMU.2022.795463
Abstract: Protection from liver-stage malaria requires high numbers of CD8+ T cells to find and kill Plasmodium -infected cells. A new malaria vaccine strategy, prime-target vaccination, involves sequential viral-vectored vaccination by intramuscular and intravenous routes to target cellular immunity to the liver. Liver tissue-resident memory (TRM) CD8+ T cells have been shown to be necessary and sufficient for protection against rodent malaria by this vaccine regimen. Ultimately, to most faithfully assess immunotherapeutic responses by these local, specialised, hepatic T cells, periodic liver s ling is necessary, however this is not feasible at large scales in human trials. Here, as part of a phase I/II P. falciparum challenge study of prime-target vaccination, we performed deep immune phenotyping, single-cell RNA-sequencing and kinetics of hepatic fine needle aspirates and peripheral blood s les to study liver CD8+ TRM cells and circulating counterparts. We found that while these peripheral ‘TRM-like’ cells differed to TRM cells in terms of previously described characteristics, they are similar phenotypically and indistinguishable in terms of key T cell residency transcriptional signatures. By exploring the heterogeneity among liver CD8+ TRM cells at single cell resolution we found two main subpopulations that each share expression profiles with blood T cells. Lastly, our work points towards the potential for using TRM−like cells as a correlate of protection by liver-stage malaria vaccines and, in particular, those adopting a prime-target approach. A simple and reproducible correlate of protection would be particularly valuable in trials of liver-stage malaria vaccines as they progress to phase III, large-scale testing in African infants. We provide a blueprint for understanding and monitoring liver TRM cells induced by a prime-target malaria vaccine approach.
Correspondence of max-flow to the absolute permeability of porous systems
Publisher: American Physical Society (APS)
Date: 14-05-2021
Local diffusion coefficient measurements in shale using dynamic micro-computed tomography
Publisher: Elsevier BV
Date: 11-2017
Evaluation of the Efficacy of ChAd63-MVA Vectored Vaccines Expressing Circumsporozoite Protein and ME-TRAP Against Controlled Human Malaria Infection in Malaria-Naive Individuals
Publisher: Oxford University Press (OUP)
Date: 21-10-2014
Reactogenicity and immunogenicity after a late second dose or a third dose of ChAdOx1 nCoV-19 in the UK: a substudy of two randomised controlled trials (COV001 and COV002)
Publisher: Elsevier BV
Date: 09-2021
Time-Lapsed Visualization and Characterization of Shale Diffusion Properties Using 4D X-ray Microcomputed Tomography
Publisher: American Chemical Society (ACS)
Date: 02-01-2018
Development of an in vitro assay and demonstration of Plasmodium berghei liver- Stage inhibition by TRAP-specific CD8+ T Cells
Publisher: Public Library of Science (PLoS)
Date: 30-03-2015
On the challenges of greyscale‐based quantifications using X‐ray computed microtomography
Publisher: Wiley
Date: 30-05-2019
DOI: 10.1111/JMI.12805
Abstract: For X-ray computed microtomography (μ-CT) images of porous rocks where the grains and pores are not fully resolved, the greyscale values of each voxel can be used for quantitative calculations. This study addresses the challenges that arise with greyscale-based quantifications by conducting experiments designed to investigate the sources of error/uncertainty. We conduct greyscale-based calculations of porosity, concentration and diffusivity from various μ-CT experiments using a Bentheimer sandstone s le. The dry sandstone is imaged overtime to test the variation of greyscale values over sequential scans due to instrumentation stability. The sandstone is then imaged in a dry and contrast-agent saturated state at low resolution to determine a porosity map, which is compared to a porosity map derived from segmented high-resolution data. Then the linearity of the relationship between the concentration of a contrast agent and its corresponding attenuation coefficient is tested by imaging various solutions of known concentration. Lastly, a diffusion experiment is imaged at low resolution under dynamic conditions to determine local diffusivity values for the sandstone, which is compared to values derived from direct pore-scale simulations using high-resolution data. Overall, we identify the main errors associated with greyscale-based quantification and provide practical suggestions to alleviate these issues. LAY DESCRIPTION: X-ray computed microtomography (CT) imaging has become an important way to study the pore space of a porous medium. Using segmented images, we can build 3D pore space models for porous media and characterize the morphology and/or run simulations on the models. So, image segmentation is a critical image processing step. However, for low resolution images where image segmentation is not possible, grayscales are directly used for quantifications such as porosity and concentration calculations. Although these types of calculations have been widely accepted and used, the uncertainties and errors associated with grayscale-based quantifications are not fully discussed. Here we specifically design experiments with X-ray CT imaging to address the challenges that arise in grayscale-based quantifications. For instance, in order to validate porosity calculation results from low resolution images (with the help of high attenuating tracer), high resolution images are also acquired, which serve as a benchmark. The errors associated with concentration calculation using grayscale values are also discussed. In addition, numerical simulations using grayscale values are performed on a diffusion experiment images with X-ray CT. The problems that arise in dynamic imaging and the subsequent numerical simulations are discussed. The experiments, calculations and discussions provide a more comprehensive understanding on grayscale-based quantifications and aid in designing better X-ray CT experiments.
Efficacy of ChAdOx1 nCoV-19 (AZD1222) vaccine against SARS-CoV-2 variant of concern 202012/01 (B.1.1.7): an exploratory analysis of a randomised controlled trial
Publisher: Elsevier BV
Date: 04-2021
Safety and immunogenicity of the ChAdOx1 nCoV-19 vaccine against SARS-CoV-2: a preliminary report of a phase 1/2, single-blind, randomised controlled trial
Publisher: Elsevier BV
Date: 08-2020
Safety and efficacy of the ChAdOx1 nCoV-19 vaccine (AZD1222) against SARS-CoV-2: an interim analysis of four randomised controlled trials in Brazil, South Africa, and the UK
Publisher: Elsevier BV
Date: 2021
An image-based coal network model for simulating hydro-mechanical gas flow in coal: An application to carbon dioxide geo-sequestration
Publisher: Elsevier BV
Date: 12-2022
Deep convolutional neural network for 3D mineral identification and liberation analysis
Publisher: Elsevier BV
Date: 06-2022
A hybrid fracture-micropore network model for multiphysics gas flow in coal
Publisher: Elsevier BV
Date: 12-2020
Single-dose administration and the influence of the timing of the booster dose on immunogenicity and efficacy of ChAdOx1 nCoV-19 (AZD1222) vaccine: a pooled analysis of four randomised trials
Publisher: Elsevier BV
Date: 03-2021
Characterization of wetting using topological principles
Publisher: Elsevier BV
Date: 10-2020
Chimpanzee adenoviral vectors as vaccines for outbreak pathogens
Publisher: Informa UK Limited
Date: 30-10-2017
Development of an in vitro Plasmodium parasite killing assay for the evaluation of cell-mediated immune responses following vaccination with pre-erythrocytic malaria vaccine candidates
Publisher: Springer Science and Business Media LLC
Date: 09-2014
Safety and immunogenicity of ChAdOx1 nCoV-19 vaccine administered in a prime-boost regimen in young and old adults (COV002): a single-blind, randomised, controlled, phase 2/3 trial
Publisher: Elsevier BV
Date: 12-2020
Probing Effective Wetting in Subsurface Systems
Publisher: American Geophysical Union (AGU)
Date: 26-02-2020
DOI: 10.1029/2019GL086151
Liquid metal synthesis solvents for metallic crystals
Publisher: American Association for the Advancement of Science (AAAS)
Date: 09-12-2022
Abstract: In nature, snowflake ice crystals arrange themselves into erse symmetrical six-sided structures. We show an analogy of this when zinc (Zn) dissolves and crystallizes in liquid gallium (Ga). The low-melting-temperature Ga is used as a “metallic solvent” to synthesize a range of flake-like Zn crystals. We extract these metallic crystals from the liquid metal solvent by reducing its surface tension using a combination of electrocapillary modulation and vacuum filtration. The liquid metal–grown crystals feature high morphological ersity and persistent symmetry. The concept is expanded to other single and binary metal solutes and Ga-based solvents, with the growth mechanisms elucidated through ab initio simulation of interfacial stability. This strategy offers general routes for creating highly crystalline, shape-controlled metallic or multimetallic fine structures from liquid metal solvents.
Cleat-scale characterisation of coal: An overview
Publisher: Elsevier BV
Date: 03-2017
Viral vectors as vaccine platforms: From immunogenicity to impact
Publisher: Elsevier BV
Date: 08-2016
DOI: 10.1016/J.COI.2016.05.014
Abstract: Viral vectors are the vaccine platform of choice for many pathogens that have thwarted efforts towards control using conventional vaccine approaches. Although the STEP trial encumbered development of recombinant human adenovirus vectors only a few years ago, replication-deficient simian adenoviruses have since emerged as a crucial component of clinically effective prime-boost regimens. The vectors discussed here elicit functionally relevant cellular and humoral immune responses, at extremes of age and in erse populations. The recent Ebola virus outbreak highlighted the utility of viral vectored vaccines in facilitating a rapid response to public health emergencies. Meanwhile, technological advances in manufacturing to support scale-up of viral vectored vaccines have helped to consolidate their position as a leading approach to tackling 'old' and emerging infections.
Phase 1/2 trial of SARS-CoV-2 vaccine ChAdOx1 nCoV-19 with a booster dose induces multifunctional antibody responses
Publisher: Springer Science and Business Media LLC
Date: 17-12-2020
In-situ hydrogen wettability characterisation for underground hydrogen storage
Publisher: Elsevier BV
Date: 03-2022
Linking continuum-scale state of wetting to pore-scale contact angles in porous media
Publisher: Elsevier BV
Date: 03-2020
DOI: 10.1016/J.JCIS.2019.11.105
Abstract: Wetting phenomena play a key role in flows through porous media. Relative permeability and capillary pressure-saturation functions show a high sensitivity to wettability, which has different definitions at the continuum- and pore-scale. We hypothesize that the wetting state of a porous medium can be described in terms of topological arguments that constrain the morphological state of immiscible fluids, which provides a direct link between the continuum-scale metrics of wettability and pore-scale contact angles. We perform primary drainage and imbibition experiments on Bentheimer sandstone using air and brine. Topological properties, such as Euler characteristic and interfacial curvature are measured utilizing X-ray micro-computed tomography at irreducible air saturation. We also present measurements for the United States Bureau of Mines (USBM) index, capillary pressure and pore-scale contact angles. Additional studies are performed using two-phase Lattice Boltzmann simulations to test a wider range of wetting conditions. We demonstrate that contact angle distributions for a porous multiphase system can be predicted within a few percent difference of directly measured pore-scale contact angles using the presented method. This provides a general framework on how continuum-scale data can be used to describe the geometrical state of fluids within porous media.
Author Correction: T cell and antibody responses induced by a single dose of ChAdOx1 nCoV-19 (AZD1222) vaccine in a phase 1/2 clinical trial
Publisher: Springer Science and Business Media LLC
Date: 21-05-2021
T cell and antibody responses induced by a single dose of ChAdOx1 nCoV-19 (AZD1222) vaccine in a phase 1/2 clinical trial
Publisher: Springer Science and Business Media LLC
Date: 17-12-2020
Multiscale characterization of shale diffusivity using time-lapsed X-ray computed tomography and pore-level simulations
Publisher: Elsevier BV
Date: 11-2019
Author Correction: Phase 1/2 trial of SARS-CoV-2 vaccine ChAdOx1 nCoV-19 with a booster dose induces multifunctional antibody responses
Publisher: Springer Science and Business Media LLC
Date: 06-05-2021
Efficacy of ChAdOx1 nCoV-19 (AZD1222) Vaccine Against SARS-CoV-2 VOC 202012/01 (B.1.1.7)
Publisher: Elsevier BV
Date: 2021
DOI: 10.2139/SSRN.3779160
An in vitro assay to measure antibody-mediated inhibition of P. berghei sporozoite invasion against P. falciparum antigens
Publisher: Springer Science and Business Media LLC
Date: 05-12-2017
DOI: 10.1038/S41598-017-17274-5
Abstract: A large research effort is currently underway to find an effective and affordable malaria vaccine. Tools that enable the rapid evaluation of protective immune responses are essential to vaccine development as they can provide selection criteria to rank order vaccine candidates. In this study we have revisited the Inhibition of Sporozoite Invasion (ISI) assay to assess the ability of antibodies to inhibit sporozoite infection of hepatocytes. By using GFP expressing sporozoites of the rodent parasite P . berghei we are able to robustly quantify parasite infection of hepatocyte cell lines by flow cytometry. In conjunction with recently produced transgenic P . berghei parasites that express P . falciparum sporozoite antigens, we have been able to use this assay to measure antibody mediated inhibition of sporozoite invasion against one of the lead malaria antigens P . falciparum CSP. By combining chimeric rodent parasites expressing P . falciparum antigens and a flow cytometric readout of infection, we are able to robustly assess vaccine-induced antibodies, from mice, rhesus macaques and human clinical trials, for their functional ability to block sporozoite invasion of hepatocytes.
Related Organisations
GlaxoSmithKline (United Kingdom)
Location: United Kingdom of Great Britain and Northern Ireland
University Of Oxford
Location: United Kingdom of Great Britain and Northern Ireland
Related Funding Activities
Discovery Projects - Grant ID: DP160104995
Start Date: 2016
End Date: 12-2018
Amount: $380,000.00
Funder: Australian Research Council
View Funded ActivityLinkage Projects - Grant ID: LP190100990
Start Date: 12-2021
End Date: 12-2024
Amount: $310,781.00
Funder: Australian Research Council
View Funded Activity