ORCID Profile
0000-0001-5363-6481
Current Organisations
Universidade Estadual Paulista (UNESP), Faculdade de Ciências, Bauru
,
Universidade Estadual Paulista Júlio de Mesquita Filho
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Publisher: Elsevier BV
Date: 04-2020
Publisher: Elsevier BV
Date: 11-2011
DOI: 10.1016/J.PHYTOCHEM.2011.07.004
Abstract: Four interconverting flavanone glycosides [(2R)- and (2S)-3',4',5,6-tetrahydroxyflavanone 7-O-β-D-glucopyranoside, and (2R)- and (2S)-3',4',5,8-tetrahydroxyflavanone 7-O-β-D-glucopyranoside], in addition to eight known flavonoids [naringenin, asebogenin, sakuranetin, 6-hydroxyluteolin 7-O-β-D-glucoside, (2R)- and (2S)-eriodictyol 7-O-β-D-glucopyranoside, aromadendrin and phloretin], three phenylpropanoid glycosides [forsythoside B, alyssonoside and verbascoside] and the epoxylignan lariciresinol 4'-O-β-D-glucopyranoside were isolated and identified in the EtOH extract of the aerial parts of Lippia salviaefolia Cham. The phytochemical study herein was guided by preliminary antioxidant tests, namely, β-carotene protection and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity. The crude extracts, their active fractions and the isolated compounds were assayed against intracellular reactive oxygen species (ROS) and human embryonic kidney HEK-293 and human melanoma M14 cancer cell growth. Aromadendrin and phloretin were able to counteract elevation of ROS induced by the oxidant t-butylhydroperoxide (t-BOOH) in HEK-293 cells, whereas phloretin strongly protected HEK-293 cells from ROS damage at 1 μM. Additionally, phloretin exhibited a significant growth inhibitory effect at 20-40 μM in both HEK-293 and M14 cells and induced a concentration dependent apoptosis at 20 μM in M14 cells, suggesting a selective action towards malignant cells. Due to their equilibria, the four interconverting flavanone glycosides were studied using 1D and 2D NMR, HPLC-CD-PDA and HRMS analyses.
Publisher: Wiley
Date: 29-10-2020
DOI: 10.1002/PCA.3006
Abstract: Soybean is one of the most important crops in the world, an important source of isoflavones, and used to treat various chronic diseases. High‐performance liquid chromatography (HPLC), associated with multivariate experiments and green solvents, is increasingly used to develop comprehensive elution methods for quality control of plants and derivatives. The work aims to establish a HPLC fingerprinting method for soybean seeds employing Green Chemistry Principles, a sustainable solvent with low toxicity, and a comprehensive experimental design that reduces the number of experiments. The fingerprinting method was optimised through Design of Experiments by evaluating seven chromatographic variables: initial percentage of ethanol (X1), final percentage of ethanol (X2), temperature (X3), percentage of acetic acid in water (X4), flow rate (X5), run time (X6), and stationary phase (X7). The dependent variable was the number of peaks ( n ). An initial factorial design for screening purposes indicated that the most significant quantitative parameters to separate soybean metabolites were X1 and X3. The conditions were optimised by a Doehlert design, to obtain a HPLC‐PAD (photodiode array detector) fingerprinting of the polar extract of soybean seeds with the markers identified by liquid chromatography electrospray ionisation tandem mass spectrometry (LC‐ESI‐MS/MS). The optimum fingerprinting method was determined as 5–55% of ethanol in 30 min, at 35°C, and flow rate of 1 mL/min, by employing a phenyl‐hexyl column (150 mm × 4.6 mm). The developed green method enabled markers of soybean to be separated and identified and could be an eco‐friendlier alternative for soybean quality control that covered seven Green Analytical Chemistry Principles.
Publisher: Springer Science and Business Media LLC
Date: 29-01-2018
Publisher: Elsevier BV
Date: 12-2020
Publisher: Springer Science and Business Media LLC
Date: 13-03-2021
Location: Brazil
Location: Brazil
No related grants have been discovered for Daniel Rinaldo.