ORCID Profile
0000-0003-4064-4188
Current Organisation
Chittagong University of Engineering and Technology
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Publisher: American Association for the Advancement of Science (AAAS)
Date: 02-09-2020
DOI: 10.1126/SCITRANSLMED.AAZ8048
Abstract: SMOC1 is a liver-secreted glucose-responsive protein that improves glycemic control through CREB-mediated suppression of hepatic glucose output.
Publisher: American Association for the Advancement of Science (AAAS)
Date: 17-12-2021
Abstract: Exercise-induced ROS mediates antioxidant defense to attenuate oxidative damage and insulin resistance in aging and obesity.
Publisher: MDPI AG
Date: 16-10-2020
DOI: 10.3390/SU12208583
Abstract: This study reviewed the aspects of the production of bioethanol from the agricultural residues available in Bangladesh. The crop residues such as rice, wheat, sugarcane, corn, cotton, jute, and sugarcane have great potential for energy generation in a sustainable and eco-friendly way in Bangladesh, as these residues are available in large quantities. Bioethanol is an alternative fuel to gasoline that provides comparable performance results. Bioethanol from these residues can be used for transportation purposes, as it does not require any major modifications to the spark-ignition engine configuration when using E5 blend (5% Ethanol mixed with 95% of the gasoline). In Bangladesh, approximately 65.36 Mt of agricultural residues are available from the major crops, from which 32 Mt bioethanol can be generated. This study is expected to provide useful concise data with regards to the beneficial utilization of agricultural residues for bioethanol production in Bangladesh.
Publisher: American Association for Cancer Research (AACR)
Date: 27-12-2022
DOI: 10.1158/1541-7786.MCR-22-0796
Abstract: Prostate cancer utilizes multiple substrates to fuel energy requirements, yet pharmacologic targeting of fatty acid uptake and oxidation reveals metabolic dependencies in localized and metastatic tumors.
Publisher: American Association for Cancer Research (AACR)
Date: 03-04-2023
DOI: 10.1158/1541-7786.22495219
Abstract: Supplementary Table 1: Correlation coefficients and P values of metabolic rates and ssGSEA score. Supplementary Table 2: Correlation coefficients and P values of metabolic rates and genetic mutations. Supplementary Table 3: Correlation coefficients and P values of metabolic rates and copy number changes. Supplementary Table 4: Correlation coefficients and P values of metabolic rates and patient pre-treatments.
Publisher: American Association for Cancer Research (AACR)
Date: 03-04-2023
DOI: 10.1158/1541-7786.C.6534778
Abstract: Abstract Cancer cells undergo metabolic reprogramming to meet increased bioenergetic demands. Studies in cells and mice have highlighted the importance of oxidative metabolism and lipogenesis in prostate cancer however, the metabolic landscape of human prostate cancer remains unclear. To address this knowledge gap, we performed radiometric ( sup /sup C) and stable ( sup /sup C) isotope tracing assays in precision-cut slices of patient-derived xenografts (PDX). Glucose, glutamine, and fatty acid oxidation was variably upregulated in malignant PDXs compared with benign PDXs. i De novo /i lipogenesis (DNL) and storage of free fatty acids into phospholipids and triacylglycerols were increased in malignant PDXs. There was no difference in substrate utilization between localized and metastatic PDXs and hierarchical clustering revealed marked metabolic heterogeneity across all PDXs. Mechanistically, glucose utilization was mediated by acetyl-CoA production rather than carboxylation of pyruvate, while glutamine entered the tricarboxylic acid cycle through transaminase reactions before being utilized via oxidative or reductive pathways. Blocking fatty acid uptake or fatty acid oxidation with pharmacologic inhibitors was sufficient to reduce cell viability in PDX-derived organoids, whereas blockade of DNL, or glucose or glutamine oxidation induced variable and limited therapeutic efficacy. These findings demonstrate that human prostate cancer, irrespective of disease stage, can effectively utilize all metabolic substrates, albeit with marked heterogeneity across tumors. We also confirm that fatty acid uptake and oxidation are targetable metabolic dependencies in human prostate cancer. Implications: Prostate cancer utilizes multiple substrates to fuel energy requirements, yet pharmacologic targeting of fatty acid uptake and oxidation reveals metabolic dependencies in localized and metastatic tumors. /
Publisher: Wiley
Date: 14-12-2021
Publisher: Elsevier BV
Date: 11-2020
Publisher: American Association for Cancer Research (AACR)
Date: 03-04-2023
DOI: 10.1158/1541-7786.22495219.V1
Abstract: Supplementary Table 1: Correlation coefficients and P values of metabolic rates and ssGSEA score. Supplementary Table 2: Correlation coefficients and P values of metabolic rates and genetic mutations. Supplementary Table 3: Correlation coefficients and P values of metabolic rates and copy number changes. Supplementary Table 4: Correlation coefficients and P values of metabolic rates and patient pre-treatments.
Publisher: The Endocrine Society
Date: 25-04-2019
Abstract: The liver is a dynamic organ that plays critical roles in many physiological processes, including the regulation of systemic glucose and lipid metabolism. Dysfunctional hepatic lipid metabolism is a cause of nonalcoholic fatty liver disease (NAFLD), the most common chronic liver disorder worldwide, and is closely associated with insulin resistance and type 2 diabetes. Through the use of advanced mass spectrometry “omics” approaches and detailed experimentation in cells, mice, and humans, we now understand that the liver secretes a wide array of proteins, metabolites, and noncoding RNAs (miRNAs) and that many of these secreted factors exert powerful effects on metabolic processes both in the liver and in peripheral tissues. In this review, we summarize the rapidly evolving field of “hepatokine” biology with a particular focus on delineating previously unappreciated communication between the liver and other tissues in the body. We describe the NAFLD-induced changes in secretion of liver proteins, lipids, other metabolites, and miRNAs, and how these molecules alter metabolism in liver, muscle, adipose tissue, and pancreas to induce insulin resistance. We also synthesize the limited information that indicates that extracellular vesicles, and in particular exosomes, may be an important mechanism for intertissue communication in normal physiology and in promoting metabolic dysregulation in NAFLD.
Publisher: Springer Science and Business Media LLC
Date: 03-02-2020
Publisher: Elsevier BV
Date: 2021
Publisher: Springer Science and Business Media LLC
Date: 23-10-2020
Publisher: American Association for the Advancement of Science (AAAS)
Date: 06-02-2019
DOI: 10.1126/SCITRANSLMED.AAU5758
Abstract: Metabolism alterations are hallmarks of cancer, but the involvement of lipid metabolism in disease progression is unclear. We investigated the role of lipid metabolism in prostate cancer using tissue from patients with prostate cancer and patient-derived xenograft mouse models. We showed that fatty acid uptake was increased in human prostate cancer and that these fatty acids were directed toward biomass production. These changes were mediated, at least partly, by the fatty acid transporter CD36, which was associated with aggressive disease. Deleting
Publisher: Elsevier BV
Date: 2021
Publisher: Elsevier
Date: 2021
Publisher: Elsevier BV
Date: 11-2020
Publisher: MDPI AG
Date: 17-10-2019
DOI: 10.3390/EN12203947
Abstract: Banana stem is being considered as the second largest waste biomass in Malaysia. Therefore, the environmental challenge of managing this huge amount of biomass as well as converting the feedstock into value-added products has spurred the demand for ersified applications to be implemented as a realistic approach. In this study, banana stem waste was experimented for bioethanol generation via hydrolysis and fermentation methods with the presence of Saccharomyces cerevisiae (yeast) subsequently. Along with the experimental analysis, a realistic pilot scale application of electricity generation from the bioethanol has been designed by HOMER software to demonstrate techno-economic and environmental impact. During sulfuric acid and enzymatic hydrolysis, the highest glucose yield was 5.614 and 40.61 g/L, respectively. During fermentation, the maximum and minimum glucose yield was 62.23 g/L at 12 h and 0.69 g/L at 72 h, respectively. Subsequently, 99.8% pure bioethanol was recovered by a distillation process. Plant modeling simulated operating costs 65,980 US$/y, net production cost 869347 US$ and electricity cost 0.392 US$/kWh. The CO2 emission from bioethanol was 97,161 kg/y and SO2 emission was 513 kg/y which is much lower than diesel emission. The overall bioethanol production from banana stem and application of electricity generation presented the approach economically favorable and environmentally benign.
Publisher: American Association for Cancer Research (AACR)
Date: 03-04-2023
DOI: 10.1158/1541-7786.22495225
Abstract: Supplementary Figure 1. Metabolic profiles of prostate cancer PDX across different pathology. Supplementary Figure 2. Percent labeling of glycolysis end products and TCA cycle intermediates following 4 hours ex vivo labelling of 13C substrates. Supplementary Figure 3. Mass isotopologue distribution of malate following glucose, glutamine, palmitate, and lactate tracing. Supplementary Figure 4. PDXOs responses following metabolic inhibitors treatment. Supplementary Figure 5. Organoids viability following 7 days treatments of metabolic inhibitors. Supplementary Figure 6. Dose responses of metabolic inhibitors treatment in PDXOs.
Publisher: Elsevier BV
Date: 08-2021
Publisher: American Physiological Society
Date: 09-2023
DOI: 10.1152/AJPENDO.00178.2023
Abstract: The present study examines the effects of exercise training on protein secretion from fast-twitch and slow-twitch muscle as well as visceral and subcutaneous adipose tissue of obese mice. Although exercise training leads to substantial remodeling of protein secretion from fast-twitch muscle, adipose tissue is completely refractory to exercise regulation.
Publisher: American Diabetes Association
Date: 07-01-2019
DOI: 10.2337/DB18-0670
Abstract: Defects in hepatic lipid metabolism cause nonalcoholic fatty liver disease and insulin resistance, and these pathologies are closely linked. Regulation of lipid droplet metabolism is central to the control of intracellular fatty acid fluxes, and perilipin 5 (PLIN5) is important in this process. We examined the role of PLIN5 on hepatic lipid metabolism and systemic glycemic control using liver-specific Plin5-deficient mice (Plin5LKO). Hepatocytes isolated from Plin5LKO mice exhibited marked changes in lipid metabolism characterized by decreased fatty acid uptake and storage, decreased fatty acid oxidation that was associated with reduced contact between lipid droplets and mitochondria, and reduced triglyceride secretion. With consumption of a high-fat diet, Plin5LKO mice accumulated intrahepatic triglyceride, without significant changes in inflammation, ceramide or diglyceride contents, endoplasmic reticulum stress, or autophagy. Instead, livers of Plin5LKO mice exhibited activation of c-Jun N-terminal kinase, impaired insulin signal transduction, and insulin resistance, which impaired systemic insulin action and glycemic control. Re-expression of Plin5 in the livers of Plin5LKO mice reversed these effects. Together, we show that Plin5 is an important modulator of intrahepatic lipid metabolism and suggest that the increased Plin5 expression that occurs with overnutrition may play an important role in preventing hepatic insulin resistance.
Publisher: American Physiological Society
Date: 02-2023
DOI: 10.1152/AJPENDO.00161.2022
Abstract: Dysregulation of hepatic lipid metabolism is a hallmark of nonalcoholic fatty liver disease. Here, we show that dual inhibition of the de novo lipogenesis enzyme, ACC, and hepatic deletion of the fatty acid transporter, CD36, was ineffective for the treatment of NAFLD in mice. This was due to a paradoxical increase in liver triglycerides with CD36 deletion resulting from decreased hepatic triglyceride secretion and increased lipogenic gene expression.
Publisher: Wiley
Date: 20-11-2021
Publisher: American Physiological Society
Date: 09-2020
DOI: 10.1152/AJPENDO.00373.2019
Abstract: Regional distribution of adipose tissue is an important factor in conferring cardiometabolic risk and obesity-related morbidity. We tested the hypothesis that human visceral adipose tissue (VAT) impairs glucose homeostasis, whereas subcutaneous glutealfemoral adipose tissue (GFAT) protects against the development of impaired glucose homeostasis in mice. VAT and GFAT were collected from patients undergoing bariatric surgery and grafted onto the epididymal adipose tissue of weight- and age-matched severe, combined immunodeficient mice. SHAM mice underwent surgery without transplant of tissue. Mice were fed a high-fat diet after xenograft. Energy homeostasis, glucose metabolism, and insulin sensitivity were assessed 6 wk later. Xenograft of human adipose tissues was successful, as determined by histology, immunohistochemical evaluation of collagen deposition and angiogenesis, and maintenance of lipolytic function. Adipose tissue transplant did not affect energy expenditure, food intake, whole body substrate partitioning, or plasma free fatty acid, triglyceride, and insulin levels. Fasting blood glucose was significantly reduced in GFAT and VAT compared with SHAM, whereas glucose tolerance was improved only in mice transplanted with VAT compared with SHAM mice. This improvement was not associated with differences in whole body insulin sensitivity or plasma insulin between groups. Together, these data suggest that VAT improves glycemic control and GFAT does not protect against the development of high-fat diet-induced glucose intolerance. Hence, the intrinsic properties of VAT and GFAT do not necessarily explain the postulated negative and positive effects of these adipose tissue depots on metabolic health.
Publisher: Wiley
Date: 10-06-2020
DOI: 10.1002/PROS.24024
Publisher: Springer Science and Business Media LLC
Date: 26-06-2021
Publisher: American Association for Cancer Research (AACR)
Date: 03-04-2023
DOI: 10.1158/1541-7786.C.6534778.V1
Abstract: Abstract Cancer cells undergo metabolic reprogramming to meet increased bioenergetic demands. Studies in cells and mice have highlighted the importance of oxidative metabolism and lipogenesis in prostate cancer however, the metabolic landscape of human prostate cancer remains unclear. To address this knowledge gap, we performed radiometric ( sup /sup C) and stable ( sup /sup C) isotope tracing assays in precision-cut slices of patient-derived xenografts (PDX). Glucose, glutamine, and fatty acid oxidation was variably upregulated in malignant PDXs compared with benign PDXs. i De novo /i lipogenesis (DNL) and storage of free fatty acids into phospholipids and triacylglycerols were increased in malignant PDXs. There was no difference in substrate utilization between localized and metastatic PDXs and hierarchical clustering revealed marked metabolic heterogeneity across all PDXs. Mechanistically, glucose utilization was mediated by acetyl-CoA production rather than carboxylation of pyruvate, while glutamine entered the tricarboxylic acid cycle through transaminase reactions before being utilized via oxidative or reductive pathways. Blocking fatty acid uptake or fatty acid oxidation with pharmacologic inhibitors was sufficient to reduce cell viability in PDX-derived organoids, whereas blockade of DNL, or glucose or glutamine oxidation induced variable and limited therapeutic efficacy. These findings demonstrate that human prostate cancer, irrespective of disease stage, can effectively utilize all metabolic substrates, albeit with marked heterogeneity across tumors. We also confirm that fatty acid uptake and oxidation are targetable metabolic dependencies in human prostate cancer. Implications: Prostate cancer utilizes multiple substrates to fuel energy requirements, yet pharmacologic targeting of fatty acid uptake and oxidation reveals metabolic dependencies in localized and metastatic tumors. /
Publisher: MDPI AG
Date: 30-05-2020
DOI: 10.3390/SU12114447
Abstract: Globally, the agriculture sector consumes a considerable portion of energy. Optimizing energy consumption and energy loss from different fuel-based types of machinery will increase the energy sustainability of this sector. Exergy analysis is a useful optimizing method that applies the thermodynamic approach to minimize energy loss. The main goal of this study is to highlight the impact of exergy loss on the energy sustainability of the agriculture sector. Hence, this study focuses on the implementation of exergy-based sustainability parameters to determine the sustainability of the agricultural sector in Bangladesh. A comprehensive analysis combining energy, exergy, and sustainability indicators was conducted based on the data obtained from 1990 to 2017. Overall energy and exergy efficiencies varied between 29.86% and 36.68% and 28.2% and 35.4%, respectively, whereas the sustainability index varied between 1.39 and 1.54. The values of relative irreversibility and lack of productivity indices from diesel fuel are higher than that of other fuel types. Maximum relative irreversibility is 0.95, whereas maximum lack of productivity is 2.50. The environmental effect factor of diesel fuel is the highest (2.47) among all the analyzed fuel types. Replacing old farming devices and selecting appropriate farming methods, appliances, and control systems will reduce exergy loss in this sector.
Publisher: American Physiological Society
Date: 09-2023
Abstract: This is the first time Australia-wide agreement has been reached on the core concepts of physiology with the Delphi method. Embedding of the core concepts will result in consistency in physiology curricula, improvements to teaching and learning, and benchmarking across Australian universities.
Publisher: American Diabetes Association
Date: 29-01-2018
DOI: 10.2337/DB17-0923
Abstract: Lipid droplets (LDs) are critical for the regulation of lipid metabolism, and dysregulated lipid metabolism contributes to the pathogenesis of several diseases, including type 2 diabetes. We generated mice with muscle-specific deletion of the LD-associated protein perilipin 5 (PLIN5, Plin5MKO) and investigated PLIN5’s role in regulating skeletal muscle lipid metabolism, intracellular signaling, and whole-body metabolic homeostasis. High-fat feeding induced changes in muscle lipid metabolism of Plin5MKO mice, which included increased fatty acid oxidation and oxidative stress but, surprisingly, a reduction in inflammation and endoplasmic reticulum (ER) stress. These muscle-specific effects were accompanied by whole-body glucose intolerance, adipose tissue insulin resistance, and reduced circulating insulin and C-peptide levels in Plin5MKO mice. This coincided with reduced secretion of fibroblast growth factor 21 (FGF21) from skeletal muscle and liver, resulting in reduced circulating FGF21. Intriguingly, muscle-secreted factors from Plin5MKO, but not wild-type mice, reduced hepatocyte FGF21 secretion. Exogenous correction of FGF21 levels restored glycemic control and insulin secretion in Plin5MKO mice. These results show that changes in lipid metabolism resulting from PLIN5 deletion reduce ER stress in muscle, decrease FGF21 production by muscle and liver, and impair glycemic control. Further, these studies highlight the importance for muscle-liver cross talk in metabolic regulation.
Publisher: American Association for Cancer Research (AACR)
Date: 03-04-2023
DOI: 10.1158/1541-7786.22495225.V1
Abstract: Supplementary Figure 1. Metabolic profiles of prostate cancer PDX across different pathology. Supplementary Figure 2. Percent labeling of glycolysis end products and TCA cycle intermediates following 4 hours ex vivo labelling of 13C substrates. Supplementary Figure 3. Mass isotopologue distribution of malate following glucose, glutamine, palmitate, and lactate tracing. Supplementary Figure 4. PDXOs responses following metabolic inhibitors treatment. Supplementary Figure 5. Organoids viability following 7 days treatments of metabolic inhibitors. Supplementary Figure 6. Dose responses of metabolic inhibitors treatment in PDXOs.
Publisher: Elsevier BV
Date: 09-2021
DOI: 10.1016/J.JHEP.2021.04.013
Abstract: Obesity often leads to non-alcoholic fatty liver disease (NAFLD), which can progress from simple steatosis (non-alcoholic fatty liver (NAFL)) to non-alcoholic steatohepatitis (NASH). The accumulation of certain lipid subtypes is linked with worsening metabolic and liver disease, however, specific changes during progression from No-NAFL to NAFL then NASH are unresolved. Herein, we characterise the liver, adipose tissue and plasma lipidome of worsening NAFLD in obesity, and evaluate the utility of plasma lipids as biomarkers of NAFLD. Venous blood, liver, visceral and subcutaneous adipose tissue s les were obtained from 181 patients undergoing bariatric surgery. NAFLD severity was assessed histologically. Lipidomic analysis was performed using liquid chromatography-tandem mass spectrometry. The liver lipidome showed substantial changes with increasing steatosis, with increased triacylglycerols, diacylglycerols and sphingolipids including ceramide, dihydroceramide, hexosyl-ceramide and GM3 ganglioside species. These lipid species were also increased in plasma with increasing hepatic steatosis and showed strong correlations with liver lipids. Adipose tissue lipidomes showed no correlation with NAFLD. There were no significant changes in liver lipids with NASH compared to NAFL. The addition of plasma lipid variables to routine markers yielded significant improvements in diagnostic accuracy for NASH (AUROC 0.667 vs. 0.785, p = 0.025). Overall, these data provide a detailed description of the lipidomic changes with worsening NAFLD, showing significant changes with steatosis but no additional changes with NASH. Alterations in the liver lipidome are paralleled by similar changes in plasma. Further investigation is warranted into the potential utility of plasma lipids as non-invasive biomarkers of NAFLD in obesity. Non-alcoholic fatty liver disease (NAFLD) is characterised by distinct changes in the liver lipidome with steatosis. The development of non-alcoholic steatohepatitis (NASH) does not result in further changes in the lipidome. Lipids within body fat do not appear to influence the lipid profile of the liver or blood. Changes in liver lipids are paralleled by changes in blood lipids. This has potential to be developed into a non-invasive biomarker for NAFLD. ACTRN12615000875505.
Publisher: Cold Spring Harbor Laboratory
Date: 14-04-2020
DOI: 10.1101/2020.04.13.039057
Abstract: Adipose tissue is a primary regulator of energy balance and metabolism. The distribution of adipose tissue depots is of clinical interest because the accumulation of upper-body subcutaneous (ASAT) and visceral adipose tissue (VAT) is associated with cardiometabolic diseases, whereas lower-body gluteal-femoral adipose tissue (GFAT) appears to be protective. There is heterogeneity in morphology and metabolism of adipocytes obtained from different regions of the body, but detailed knowledge of the constituent proteins in each depot is lacking. Here, we determined the human adipocyte proteome from ASAT, VAT and GFAT using high-resolution SWATH mass spectrometry proteomics. We quantified 4220 proteins in adipocytes, and 2329 proteins were expressed in all three adipose depots. Comparative analysis revealed significant differences between adipocytes from different regions (6 and 8% when comparing VAT vs. ASAT and GFAT, 3% when comparing ASAT vs. GFAT), with marked differences in proteins that regulate metabolic functions. The VAT adipocyte proteome was overrepresented with proteins of glycolysis, lipogenesis, oxidative stress and mitochondrial dysfunction. The GFAT adipocyte proteome predicted activation of PPARα, fatty acid and BCAA oxidation, enhanced TCA cycle flux and oxidative phosphorylation, which was supported by metabolomic data obtained from adipocytes from the same patient donors. Together, this proteomic analysis provides an important resource and novel insights that enhance the understanding of metabolic heterogeneity in the regional adipocytes of humans.
Location: Bangladesh
No related grants have been discovered for Matthew Watt.