ORCID Profile
0000-0002-9307-4126
Current Organisation
Banaras Hindu University
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Publisher: Springer Science and Business Media LLC
Date: 17-04-2017
Publisher: Oxford University Press (OUP)
Date: 05-07-2018
DOI: 10.1093/BFGP/ELY020
Abstract: Plants as bioreactors have been widely used to express efficient vaccine antigens against viral, bacterial and protozoan infections. To date, many different plant-based expression systems have been analyzed, with a growing preference for transient expression systems. Antibody expression in erse plant species for therapeutic applications is well known, and this review provides an overview of various aspects of plant-based biopharmaceutical production. Here, we highlight conventional and gene expression technologies in plants along with some illustrative ex les. In addition, the portfolio of products that are being produced and how they relate to the success of this field are discussed. Stable and transient gene expression in plants, agrofiltration and virus infection vectors are also reviewed. Further, the present report draws attention to antibody epitope prediction using computational tools, one of the crucial steps of vaccine design. Finally, regulatory issues, biosafety and public perception of this technology are also discussed.
Publisher: Bentham Science Publishers Ltd.
Date: 06-07-2017
Publisher: Springer Science and Business Media LLC
Date: 08-03-2016
DOI: 10.1007/S00894-016-2935-Y
Abstract: Recently inulinase has regained interest due to its usage in the production of fructooligosaccharides, biofuels, and in pharmaceutical industries. Inulinases properties are experimentally reported by nomerous studies but their characteristics are just partially explained by only a few computational investigations. In the present study we have investigated exoinulinase and endoinulinase from different microbial sources toward their catalytic activity. Docking and molecular dynamic (MD) simulation were carried out for microbial endoinulinase and exoinulinase docked with 1-kestose and fructose-6-phosphate respectively. Pseudomonas mucidolens (-7.42 kcal mol(-1) binding energy), docked with fructose-6-phosphate, was recorded as the most favorable binding energy, Pseudomonas mucidolens made hydrogen bonds with fructose-6-phosphate and the amino acids involved were arginine 286, tryptophan 158, and isoleucine 87. After the simulation only tryptophan 158 remained bonded and additionally valine 156 made hydrogen bonds with fructose-6-phosphate. Aspergillus niger docked with 1-kestose was bonded with the involvement of threonine 271, aspartate 285, threonine 288, and proline 283, after the simulation aspartate 285 was retained till the end of the simulation. The present study thus refers to the indication of depicting binding analysis of microbial inulinases.
Publisher: Springer Science and Business Media LLC
Date: 30-10-2017
Publisher: Springer Science and Business Media LLC
Date: 18-06-2018
Publisher: Elsevier BV
Date: 03-2021
Publisher: Elsevier BV
Date: 02-2021
Publisher: Springer Science and Business Media LLC
Date: 21-01-2012
Publisher: Oxford University Press (OUP)
Date: 02-07-2014
DOI: 10.1093/BFGP/ELU023
Abstract: Different systems biology approaches may have a significant consequence in deciphering microbial interactions. Here, we endeavor to summarize, epigrammatic description of sophisticated techniques and software that provides an enhanced understanding of metagenomics data analysis. Apparently, such techniques are helpful to catalog various analysis categories and components that add appraisal to understand this approach. In addition, the constructions of metabolic networks of various genes present in human gut microbiome also give significant directions for determining the topological features of target enzymes.
Publisher: Springer Science and Business Media LLC
Date: 04-2015
No related grants have been discovered for Pratyoosh Shukla.