ORCID Profile
0000-0003-3376-1677
Current Organisations
Kupat Holim Meuhedet
,
University of New England School of Environmental and Rural Science
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Publisher: Elsevier BV
Date: 05-2015
DOI: 10.3382/PS/PEV035
Abstract: The use of a yeast cell wall extract derived from Saccharomyces cerevisiae (Actigen(®)) has been proposed as an alternative to in-feed antibiotics. This experiment was conducted to investigate the efficacy of yeast cell extract as an alternative to zinc bacitracin or salinomycin using a necrotic enteritis challenge model. A feeding study was conducted using 480-day-old male Ross 308 chicks assigned to 48 floor pens. A 2 × 4 factorial arrangement of treatments was employed. The factors were: challenge (- or +) and feed additive (control, zinc bacitracin at 100/50 mg/kg, yeast cell wall extract at 400/800/200 mg/kg, or salinomycin at 60 mg/kg in starter, grower, and finisher, respectively). Diets based on wheat, sorghum, soybean meal, meat and bone meal, and canola meal were formulated according to the Ross 308 nutrient specifications. Birds were challenged using a previously established protocol (attenuated Eimeria spp oocysts) on d 9 and 10(8) to 10(9) Clostridium perfringens (type A strain EHE-NE18) on d 14 and 15). Challenged and unchallenged birds were partitioned to avoid cross contamination. Challenged birds had lower weight gain, feed intake and livability compared to unchallenged birds on d 24 and d 35 (P < 0.05). Birds given zinc bacitracin, yeast cell wall extract, or salinomycin had improved weight gain and livability when compared to control birds given no additives. Challenge × additive interactions were observed for feed intake and weight gain on d 24 and d 35 (P < 0.01). The additives all had a greater positive impact on feed intake, weight gain, and livability in challenged than unchallenged birds. All challenged birds showed higher necrotic enteritis lesion scores in the small intestine sections when compared to unchallenged birds (P < 0.01). Birds fed yeast cell wall extract exhibited increased villus height, decreased crypt depth, and increased villus:crypt ratio when challenged. Yeast cell wall extract, zinc bacitracin, and salinomycin were effective in preventing performance decline from necrotic enteritis in the current study. This study indicates that yeast cell wall extract has promise as a tool for controlling necrotic enteritis.
Publisher: Elsevier BV
Date: 09-2017
Publisher: Elsevier BV
Date: 06-2019
Publisher: Elsevier BV
Date: 09-2023
Publisher: Elsevier BV
Date: 06-2015
Publisher: Elsevier BV
Date: 2019
DOI: 10.3382/PS/PEY383
Abstract: Ascaridia galli is one of the most abundant nematode parasites in poultry. A. galli infections can significantly impact the profitability of egg farms and have negative implications for bird health and welfare. The main objectives of this study were to determine whether A. galli specific antibodies in egg yolks can be used to detect prior or current exposure to A. galli in laying hens, and to distinguish between eggs obtained from caged and free-range hens. Twenty-two laying hen flocks from different production systems (10 free-range, 2 barn-housed, and 9 caged flocks) were enrolled in the study. An in-house enzyme-linked immunosorbent assay was used to analyze levels of A. galli specific antibodies in yolk. The numbers of A. galli eggs in hen excreta were also determined in a subset of farms. Free-range flocks had higher and also more variable levels of anti-A. galli antibodies in the egg yolk compared to those of the cage flocks (0.50 ± 0.39 vs. 0.16 ± 0.13 OD units) (P < 0.001). Results also confirmed that excreta from free-range and barn-housed flocks contained higher numbers of A. galli eggs than did excreta from caged flocks in which no A. galli eggs were detected. In conclusion, analysis of anti-A. galli antibodies in the egg yolk can be used to detect worm exposure in commercial layer flocks. However, the method used in this study cannot be used in isolation to distinguish between eggs from cage and free-range production systems as anti-A galli antibodies were detected in egg yolk s les from all production systems, and the range of antibody levels overlapped between production systems.
Publisher: Springer Science and Business Media LLC
Date: 20-03-2018
Publisher: Informa UK Limited
Date: 02-10-2021
Publisher: Elsevier BV
Date: 2020
DOI: 10.3382/PS/PEZ554
Publisher: Elsevier BV
Date: 06-2018
DOI: 10.3382/PS/PEY068
Abstract: A study was conducted to determine the performance, egg quality, and liver lipid reserves of laying hens exposed to ranges contaminated with Ascaridia galli. Sixteen-week-old Lohmann Brown laying hens (n = 200) were ided into 4 treatments with 5 replicates containing 10 hens per pen. Hens of treatment 1 [negative control (NC)] ranged on a decontaminated area, and hens of treatments 2 (low infection) and 3 (medium infection) ranged on areas previously contaminated by hens artificially infected with 250 and 1,000 embryonated A. galli eggs, respectively. The hens of treatment 4 [positive control (PC)] ranged on areas previously contaminated by hens artificially infected with 2,500 embryonated A. galli eggs, and in addition these hens were orally inoculated with 1,000 embryonated eggs. Results indicated that hens of the medium infection group had a higher number of intestinal A. galli worms and A. galli eggs in the coprodeum excreta (43.9 ± 4.0 and 3,437 ± 459 eggs/g) compared to hens of the low infection group (23.8 ± 4.0 and 1,820 ± 450 eggs/g) (P 0.05). Egg production, egg mass, feed intake, and feed conversion ratio (FCR) were not affected by A. galli infection (P > 0.05). Egg quality parameters (egg weight, shell reflectivity, shell weight, shell thickness, shell percentage, shell breaking strength, deformation, albumen height, Haugh unit, and yolk score) were not affected by A. galli infection (P > 0.05). Highly infected hens had lower liver lipid content (2.72 ± 0.51 g) compared to uninfected hens (4.46 ± 0.58 g, P < 0.01). The results indicate that exposure to ranges contaminated with A. galli resulted in infection of the ranging hens, but this did not affect egg production or egg quality. Infection with A. galli lowered the liver lipid reserves of the host significantly, suggesting infected hens use more energy reserves for maintenance and production.
Publisher: Elsevier BV
Date: 05-2017
DOI: 10.3382/PS/PEW401
Abstract: Eleven canola seed (CS) s les were collected from different commercial feedmills and crushing plants in Australia and analyzed for nutrient profile. Six of these s les were selected to determine the effect of seed chemical composition and pellet temperature (PT) during steam pelleting on apparent metabolizable energy corrected for nitrogen (AMEn) values of CS for broiler chickens using a 6 × 2 factorial arrangement of treatments. The CS s les were incorporated into a corn-soybean meal diet at 15% by replacing energy-yielding ingredients, and diets were steam pelleted at either 75 or 90°C. A total of 420 18-day-old male broiler chicks (Ross 308) was assigned to 14 experimental diets replicated 6 times, with 5 chicks per cage. After a 5-day diet acclimation period from d 18 to 22, excreta were collected for 72 h using the substitution method to determine AME and AMEn. There was no interaction of seed source and PT for ileal digestible energy (IDE), AME, or AMEn values of CS (P > 0.05). PT did not affect energy availability of CS (P > 0.05) but increasing the PT improved the pellet durability index of the diets by approximately 5.0 percentage points. A significant effect of seed source was detected for all the energy utilization values of CS (P < 0.05). The IDE, AME, and AMEn values of seed s les ranged from 5,239 to 5,645, 4,728 to 5,071, and 4,501 to 4,791 kcal/kg of DM, respectively. The mean AMEn values were 4,664 kcal/kg of DM, indicating a 5.7% reduction compared with AME values. There was a negative correlation between protein and fat content of the seeds (r = -0.93, P = 0.001), and, consequently, AMEn (r = -0.32, P = 0.009). AMEn values were positively correlated with fat content of CS (r = 0.649, P = 0.001). These results indicate that fat and protein content and fiber components may have a considerable effect on energy availability of CS for broiler chickens.
Publisher: Elsevier BV
Date: 11-2017
DOI: 10.3382/PS/PEX212
Abstract: Two broiler chicken experiments were conducted to investigate the effects of canola meal (CM) replacing soybean meal (SBM) in diets supplemented with carbohydrase and protease on performance and partitioning of energy. First, a 2 × 2 × 2 factorial arrangement of treatments was employed to evaluate: protein meals (CM vs. SBM), carbohydrase (none or 300 mg/kg), protease (none or 200 mg/kg), and their interactions. Each treatment was fed to 6 replicated pens of 16 male broilers (Ross 308) from d 10 to 35. In the second experiment, 32 broiler chicks were used in a 2 × 2 factorial arrangement to investigate CM and carbohydrase effects on energy partitioning. Birds were transferred into 16 closed-circuit calorimeter chambers (4 chambers/diet 2 birds/chamber) to measure heat production (HP), metabolizable and net energy (NE) by gaseous exchange, and total excreta collection from d 25 to 28. There were no 3-way interactions among experimental factors for any of the performance parameters measured. Birds given CM diets consumed less feed, had lower BW, and exhibited higher FCR compared to the control birds (P < 0.01). Both enzymes, alone or in combination, improved final BW and FCR (P < 0.05). There was an interaction between carbohydrase and protease for FCR over the grower period (P < 0.01), in which the combination of the enzymes resulted in further improvement of FCR. Energy, DM, and crude protein digestibility values were higher in control birds (P < 0.05). There was an interaction of protein meal and carbohydrase for HP, respiratory quotient (P < 0.05), and NE:ME ratio of the diets (P = 0.06). Inclusion of CM without carbohydrase increased HP and decreased NE and NE:ME ratio of the diets (P < 0.05). Carbohydrase decreased HP and increased retained energy (P = 0.06) and NE and NE:ME ratio (P < 0.05). In conclusion, high CM in the diet negatively affects growth performance through reduction in feed consumption, nutrient digestibility, and NE of the diet, which could partly be restored by enzyme supplementation.
Publisher: Elsevier BV
Date: 2018
DOI: 10.3382/PS/PEX257
Abstract: Necrotic enteritis (NE) challenge and high dietary sodium (from sodium chloride) level on odor flux from broiler litter was investigated using 160 day-old Ross 308 male chicks randomly assigned to 4 dietary treatments with 4 replicates of 10 birds each. A 2 × 2 factorial arrangement of treatments was employed. Factors were: presence or absence of NE challenge and normal (1.6 g/kg) or high (4.0 g/kg) dietary sodium (Na) level. On d 20, odorants were collected from litter headspace with a flux hood and measured using selected ion flow tube mass spectrometry (SIFT-MS). On d 33, while challenge did not lead to higher mortality, it reduced feed intake by 5.48% (P < 0.05) and body weight gain by 9.02% (P < 0.01) and worsened FCR by 5 points (P < 0.01), indicating subclinical necrotic enteritis occurred in challenged birds. Challenge increased (P < 0.01) litter moisture and litter headspace concentrations of dimethyl sulfide (P < 0.05), propyl mercaptan (P < 0.05), total butanols (P < 0.05), acetoin (P < 0.01), skatole (P = 0.05), butyric acid (P < 0.05), and methyl amine (P < 0.05) and tended to increase concentrations of ethyl mercaptan (P = 0.07), carbon disulfide (P = 0.09), indole (P = 0.10), and formic acid (P = 0.10) compared to the unchallenged group. The birds fed a high Na diet produced higher litter moisture (P < 0.01) and higher litter headspace concentration of sulfur compounds and phenol (P < 0.01) compared to those fed a normal Na diet. In the birds fed a high Na diet, challenge increased the litter flux of some additional odorants, which included 2,3-butanedione (P < 0.05), acetic acid (P < 0.01), propionic acid (P < 0.01), isobutyric acid (P < 0.01), isovaleric acid (P < 0.01), pentanoic acid (P < 0.05), 2-butanone (P < 0.05), and 3-methyl-1-butanol (P < 0.05). These findings suggest that both a high Na diet and sub-clinical NE increase the odor nuisance potential of broiler farms.
Publisher: Informa UK Limited
Date: 04-03-2017
DOI: 10.1080/00071668.2017.1403566
Abstract: 1. The objective of this study was to investigate the effect of range type, multi-enzyme applications, and a combination of benzoic acid (BA) and essential oils (EO) on the productive performance, organ weight and egg quality of free-range laying hens. 2. Three hundred laying hens were evaluated for the short-term (6 weeks) and long-term (12 weeks) effects of range type (G = no pasture, P = pasture) and feed additives (T1 = control T2 = betaglucanase ectinase rotease T3 = BA/EO). Body weight, feed intake (FI), feed conversion ratio (FCR), egg production (EP), digestive organ weight, and egg quality (EQ) were evaluated. Data were analysed using SPSS 2.2 in a 2×2×3 factorial arrangement. 3. Hens that ranged on pasture were significantly heavier (2043 g vs. 1996 g p < 0.001), laid heavier eggs (61.9 g vs. 60.3 g p < 0.001) and produced darker yolk colour (4.3 vs. 7.0 p < 0.001) compared to hens ranged on gravel. Hens fed T2 were significantly heavier (2050 g) compared to hens fed T1 (2005 g) or T3 (2008 g). Organ weights (gizzard, liver and pancreas) were significantly heavier in hens ranged on pasture (16.8 g/kg BW, 22.3 g/kg BW and 1.89 g/kg BW, respectively) compared to hens ranged on gravel (14.2 g/kg BW, 21.7 g/kg BW and 1.83 g/kg BW, respectively). Over time, body weight (1970-2070 g p < 0.001) and egg weight (59.5-62.8 g p < 0.001) increased, FI (123-120 g p = 0.024) was reduced and FCR (2.36-2.10 p = 0.002) improved 4. In conclusion, hens housed on pasture and fed multi-enzyme supplemented diets had significantly heavier body weight and produced heavier eggs with darker yolk colour. Pasture intake and enzyme supplementation increased digestive organ weight significantly.
Publisher: Elsevier BV
Date: 12-2021
Publisher: Elsevier BV
Date: 11-2015
DOI: 10.3382/PS/PEV222
Abstract: The net energy (NE) value may be a better measure than apparent metabolizable energy (ME) of the effect of supplemental phytase on energy utilization in broilers. The present study was conducted to assess the impact of 3 microbial phytases supplemented at an unconventionally high level (1,000 FTU/kg feed) on performance and NE of broilers using the indirect calorimetric method (IC). Four treatments included: 1) Control, formulated to be deficient in ME (12.35 MJ/kg in the starter diet 12.56 MJ/kg in the grower diet), calcium (0.72% in the starter diet 0.60% in the grower diet), and available phosphorus (0.25% in the starter diet 0.20% in the grower diet) 2) control + intrinsically thermostable phytase A 3) control + intrinsically thermostable phytase B and 4) control + coated phytase C. A completely randomized design was employed. A total of 384 male broiler chicks were used, and each treatment had 6 replicates with 16 birds per replicate. The birds were reared until d 21 in floor pens with hardwood shavings. Thirty-two birds (8 birds per treatment) were randomly selected to determine heat production and NE (from 25-28 d) following a 3-d acclimatization in the respiratory chambers. Performance results at d 21 showed that supplementation with either of the 3 phytases improved body weight (P < 0.001) and feed intake (P < 0.05), and increased the relative weights of tibia ash (P < 0.05) and toe ash (P < 0.01). Phytases A and B increased the NE value of the diet (P < 0.05). It may be concluded that the negative effects imposed by calcium and available phosphorus down-specification can be compensated by phytase supplementation in general, and intrinsically thermostable phytases improve the ME and NE value. However, phytase did not reduce heat production, heat increment, or increase NE:ME in birds.
Publisher: Elsevier BV
Date: 03-2014
DOI: 10.1016/J.VETMIC.2014.01.007
Abstract: It is widely established that a high-protein fishmeal supplemented starter diet and Eimeria infection can predispose birds to the development of clinical necrotic enteritis symptoms following Clostridium perfringens infection. However, it has not been clearly established what changes these treatments cause to predispose birds to succumb to necrotic enteritis. We analysed caecal microbiota of 4 groups of broilers (n=12) using deep pyrosequencing of 16S rDNA licons: (1) control chicks fed a control diet, (2) Eimeria infected chicks fed control diet, (3) chicks fed fishmeal supplemented diet and lastly (4) both fishmeal fed and Eimeria infected chicks. We found that the high-protein fishmeal diet had a strong effect on the intestinal microbiota similar to the previously reported effect of C. perfringens infection. We noted major changes in the prevalence of various lactobacilli while the total culturable Lactobacillus counts remained stable. The Ruminococcaceae, Lachnospiraceae, unknown Clostridiales and Lactobacillaceae families were most affected by fishmeal with increases in a number of operational taxonomic units (OTUs) that had previously been linked to Crohn's disease and reductions in OTUs known to be butyrate producers. Eimeria induced very different changes in microbiota Ruminococcaceae groups were reduced in number and three unknown Clostridium species were increased in abundance. Additionally, Eimeria did not significantly influence changes in pH, formic, propionic or isobutyric acid while fishmeal induced dramatic changes in all these measures. Both fishmeal feeding and Eimeria infection induced significant changes in the gut microbiota these changes may play an important role in predisposing birds to necrotic enteritis.
Publisher: Elsevier BV
Date: 04-2018
DOI: 10.3382/PS/PEX403
Abstract: Improving diet digestibility is important to the broiler industry. Therefore, this study focused on optimizing the physical structure of feed ingredients and addition of dietary fiber as strategies to improve nutrient digestibility in low and high sodium diets. A total of 672 day-old Ross 308 male broilers was allocated to 48 pens using a 2 × 2 × 2 factorial arrangement of treatments with 2 particle sizes of corn (coarse 3,576 μm or fine 1,113 μm geometric mean diameter), 2 levels of sugarcane bagasse (SB) (0 or 2%), and 2 levels of Na (0.16 or 0.4%). Protein digestibility coefficient was measured using pooled distal ileal digesta of 3 birds per pen on d 24. Meanwhile, starch and gross energy digestibility coefficients were measured using pooled duodenal, distal jejunal, and distal ileal digesta of 3 birds per pen on d 24. Coarsely ground corn (CC) resulted in improved ileal protein digestibility (P < 0.05). Addition of 2% SB increased starch digestibility in the duodenum (P < 0.05), distal jejunum (P < 0.001), and distal ileum (P < 0.001), and increased protein digestibility in distal ileum (P < 0.01). A significant particle size × SB × Na interaction was observed for ileal energy digestibility (P < 0.05). The SB increased ileal energy digestibility only in birds fed the diet with finely ground corn (FC) and 0.16% Na. These findings demonstrate that SB and CC are able to improve nutrient digestibility. It can be recommended for the poultry industry to use SB and coarsely ground corn in feed to improve the utilization of nutrients.
Publisher: Elsevier BV
Date: 09-2017
DOI: 10.3382/PS/PEX129
Abstract: An in vitro study was carried out to investigate the volatile odorous metabolites produced by necrotic enteritis inducing Clostridium perfringens (Cp) type A field strain (EHE-NE18) cultured in two broth media (thioglycollate broth, brain heart infusion broth) at three levels (0, 103 cfu/mL, 106 cfu/mL) using 2 × 3 factorial arrangement of treatments, with each replicated three times. The culture media with and without Cp was freshly prepared on the day of measurement and the culture headspace concentration of odorants was measured using selected ion-flow tube mass spectrometry (SIFT-MS). The results showed that media type and Cp level affected the concentration of a range of volatile odorous metabolites including sulfur compounds, alcohols, ketones, amines, and carboxylic acids. Thus, Cp contributes to the production of a wide range of odorous metabolites that can impart noxious smell during infection.
Publisher: Elsevier BV
Date: 07-2017
DOI: 10.3382/PS/PEX008
Abstract: The effectiveness of the addition of oat hulls (OH) as an insoluble fiber for improving nutrient digestibility and performance of birds fed diets containing full-fat canola seed (CS) was studied. A 2 × 2 × 2 factorial arrangement of treatments was used to assess the main effects of canola source (CS vs canola meal plus oil as control), OH (0 or 3%), pellet temperature (75 and 90°C) and their interactions. A total of 576 male day-old Ross 308 chickens were assigned to 8 experimental treatments, each replicated 6 times (12 birds per replicate). All birds were fed a same commercial starter diet for the first 10 d of age. Canola meal and canola oil in the control diets were replaced with CS at 11.6% and 13.5% in the grower (d 10 to 24) and finisher (d 24 to 35) diets, respectively. An interaction was observed between canola source and OH led to improved body weight gain (P < 0.01) and FCR (P < 0.05) in birds fed the combination of CS and OH in grower phase. Pelleting temperature at 75 vs 90°C did not affect performance of broilers. Birds fed diets containing OH had heavier gizzards at 24 and 35 d of age. Inclusion of CS in the diets depressed fat digestibility at d 24 (P < 0.001) and AME of the grower diets. At d 35, there was a significant interaction (P < 0.05) between CS and pellet temperature where birds fed CS diets pelleted at 75°C had higher fat digestibility than birds fed CS pelleted at 90°C. Regardless of canola source or pellet temperature, OH increased fat utilization at d 35 (P < 0.001) but had no effect on AME of the grower diets. In conclusion, CS can replace supplemental oil in broiler diets when an adequate source of insoluble fiber is included in the diet, which may help to maintain feed intake of broilers fed CS in steam-pelleted diets.
Publisher: Elsevier BV
Date: 06-2017
DOI: 10.3382/PS/PEW473
Abstract: Broiler chickens are commonly placed on wood shavings as litter, but alternative litter sources are required due to the scarcity of wood shavings in many parts of the world. This study aimed to compare pelleted straw, chopped wheat straw, wood shavings, rice hulls, and shredded paper as litter candidates. Three-hundred-sixty Ross 308 one-day-old male chicks were used in this study. There were 5 litter treatments with 6 replicate pens, each with 12 birds. The feed conversion ratio (FCR) of birds reared on pelleted straw was improved compared (P < 0.05) to that of birds raised on rice hulls, whereas it did not differ for birds placed on wood shavings, rice hulls, chopped straw, or shredded paper. It was observed that the birds reared on wood shavings had higher relative gizzard weight at d 24 compared to those reared on pelleted straw (P < 0.05). Gizzard pH and measured cecal bacterial groups were not affected by the type of bedding material. Cecal bacterial groups measured at d 10 were not affected by bedding material. Birds reared on pelleted wheat straw had a lower incidence of footpad lesions than those on chopped straw and shredded paper on d 24 (P < 0.001) and 29 (P < 0.01). Litter source did not affect the occurrence of breast blisters at d 24, 29, or 35. On d 24, 29, and 35, pelleted straw litter was less caked than chopped straw and shredded paper (P < 0.001) whereas no significant differences were observed among pelleted straw, wood shavings, and rice hulls. The study demonstrated the potential benefits to using pelleted wheat straw as a bedding material. Further assessment of pelleting of wheat straw and other materials on broiler health, performance, and welfare are needed to determine the economic benefits of pelleted litter.
Publisher: Elsevier BV
Date: 04-2018
DOI: 10.3382/PS/PEX405
Abstract: A 3-factor, 3-level Box-Behnken design was used to investigate the interaction effect of dietary digestible lysine (dLys, 9.5, 10.5, 11.5 g/kg), apparent metabolizable energy (AMEn, 12.77, 13.19, 13.61 MJ/kg) and available P (avP, 3.0, 4.0, 5.0 g/kg) levels on performance and amino acid (AA) digestibility of Ross 308 male broilers (n = 1,050) from d 14 to 34. The design consisted of 15 treatments each replicated 5 times with 12 birds per replicate. On d 34, 3 birds were s led from each pen to collect ileal digesta (pooled per pen) to analyze AA. Response surface was fitted by first-, second-, or third-degree polynomial regressions in JMP statistical software v. 12.0.1. Feed intake (FI), weight gain (WG) and feed conversion ratio (FCR) were affected by dLys (linear and quadratic, P < 0.01), AMEn (linear, P < 0.01) and AMEn × avP (P < 0.01). Increased dLys increased FI but increased AMEn decreased FI in the birds fed the low-avP diet. However, when the avP level in the diet was increased, FI decreased to 13 MJ/kg AMEn and remained constant thereafter. Increased dLys increased WG whereas an increase in AMEn decreased WG in the birds fed the low-avP diet but had no effect on WG in those fed the high-avP diet. Increased dLys decreased FCR whereas increased AMEn decreased FCR in the birds fed the low-avP diet but had no effect on FCR in those fed the high-avP diet. Increased dLys increased breast yield percentage (linear, P < 0.01 and quadratic, P < 0.05) whereas increased AMEn decreased breast yield percentage (linear, P < 0.01). Dietary levels of dLys or avP had positive, linear effects on apparent ileal digestibility (AID) of methionine (P < 0.01) and threonine (P 0.05). These results indicate that increasing dLys levels above current industry standard would improve broiler performance irrespective of AMEn or avP levels of the diet.
Publisher: Elsevier BV
Date: 10-2017
DOI: 10.3382/PS/PEX164
Abstract: Necrotic enteritis (NE) causes significant economic losses in the broiler chicken industry, especially in birds raised without in-feed antibiotics. A standardized blend of plant-derived isoquinoline alkaloids (IQA) derived from Macleaya cordata has shown to have anti-inflammatory potency and promoted animal productivity. This study investigated the effects of IQA supplementation on broiler chickens under NE challenge. A 2 × 2 factorial arrangement of treatments was employed with factors: NE challenge (no or yes), and additives (no additive or IQA at 0.15 g/kg) in starter, grower, and finisher diets. Birds were challenged with Eimeria spp. on d 9 and 108 to 109Clostridium perfringens on d 14. Each treatment had 7 replicate floor pens with 17 birds each. NE challenge negatively affected growth performance, livability, and carcass traits. Regardless of challenge, IQA increased feed intake and gain on d 24 (P < 0.05) and 35 (P < 0.01) and improved FCR (P < 0.05), flock uniformity (P < 0.01) and breast meat yield (P < 0.001) on d 35. Supplementation of IQA also reduced lesions in the duodenum (P < 0.05), jejunum (P < 0.001), and ileum (P < 0.001). This study suggests that IQA may protect broilers from NE indicating its role as a promising antibiotic alternative.
Publisher: Springer Science and Business Media LLC
Date: 12-2018
Publisher: Elsevier BV
Date: 09-2017
DOI: 10.3382/PS/PEX123
Abstract: Measures to improve gut health and nutrient digestibility have been sought due to in-feed antibiotics being phased out in poultry. The appropriate physical structure of feed ingredients and addition of dietary fiber may be beneficial in enhancing gut health in poultry. In this study, the effect of a lignocellulose-rich fiber source and corn particle size on broiler performance, gizzard development, nutrient digestibility, cecal microflora, and litter quality was evaluated. A total of 684 day-old male Ross 308 chicks were randomly allocated to 6 treatments with 6 replicate pens, each housing 19 birds. A 2 × 3 factorial arrangement of treatments was applied with the factors of: corn particle size (coarse: 2,982 μm or fine: 941 μm geometric mean diameter), and 3 levels of lignocellulose (0%, 1% or 2%). Significant particle size × fiber interaction was observed for feed conversion ratio (FCR) at d 10 (P < 0.05). The birds fed coarsely ground corn (CGC) had lower FCR than those fed finely ground corn (FGC) only at 2% of lignocellulose but not at 1% or no lignocellulose addition. Birds fed FGC were heavier (P < 0.001) at d 10. In contrast, at d 24 and 35, birds fed CGC had lower FCR than those fed FGC (P < 0.05). Ileal gross energy and protein digestibility increased in birds fed CGC compared with those fed FGC at d 24 (P < 0.05). Relative gizzard weight was higher (P < 0.05) on d 24 and 35 in birds fed CGC as compared to those fed FGC. Birds consumed 2% dietary lignocellulose had decreased counts of cecal Clostridium spp. compared to those with 1% lignocellulose (P < 0.05) at d 24. On d 35, both levels of lignocellulose had significantly decreased (P < 0.05) litter moisture content compared to the control. In conclusion, birds fed pelleted diets containing CGC exhibited improved FCR, and increased nutrient digestibility, which may have been caused by larger gizzards. Furthermore, dietary lignocellulose addition is beneficial to litter quality.
Publisher: Elsevier BV
Date: 04-2018
DOI: 10.3382/PS/PEX444
Abstract: Necrotic enteritis (NE) causes significant economic losses in the broiler chicken industry, especially in birds raised without in-feed antibiotics. L-glutamine (Gln) is an amino acid that may compensate for metabolic losses from infection and improve the intestinal development. This study investigated the effects of dietary Gln (10 g/kg) supplementation on growth performance, intestinal lesions, jejunum morphology, and serum biochemical indices of broiler chickens during NE challenge. The study employed a factorial arrangement of treatments with factors: NE challenge, no or yes dietary Gln inclusion, 0 g/kg in starter (S), d 0 to 10, grower (G) d 10 to 24, and finisher (F) d 24 to 35 10 g/kg in S, G, F, or 10 g/kg in S, G only. Each treatment was replicated in 6 floor pens with 17 birds per pen as the experimental unit for performance and 2 birds for other measurements. Challenge significantly reduced bird performance, increased incidence of intestinal lesions, and affected intestinal development and serum biochemical indices. Regardless of challenge, Gln supplementation increased gain (P < 0.05), feed intake (P < 0.05), and decreased FCR (P < 0.05) on d 24. On d 35, Gln improved gain (P < 0.05) and FCR (P < 0.001) whereas withdrawing Gln from finisher tended to diminish the beneficial effect on weight gain but not FCR. Dietary Gln reduced lesion scores in the jejunum (P < 0.01) and ileum (P < 0.01) in challenged birds. On d 16, Gln increased villus height to crypt depth ratio in unchallenged birds (P < 0.05) and reduced crypt depth of challenged birds on d 24 (P < 0.05). Regardless of challenge, supplementation with Gln reduced crypt depth on d 16 (P < 0.05), and increased villus height (P < 0.01) and the villus height to crypt depth ratio (P < 0.001) on d 24. Dietary Gln lowered serum uric acid level regardless of challenge (P < 0.05). The current study indicates that dietary Gln alleviates adverse effects of NE and may be useful in antibiotic-free diets.
Publisher: CSIRO Publishing
Date: 2016
DOI: 10.1071/AN15333
Abstract: The broiler industry has experienced remarkable improvements in productivity owing to high priority placed on genetic programming, nutrition and management practices. Nutrition of the meat chicken is driven by the need to satisfy the constantly increasing genetic potential, resulting in the shortening of the rearing time required to get the bird to market weight. The concept of feeding broilers a more specialised pre-starter diet for the first 10–14 days of age is gaining interest, as this now accounts for more than 20% of the time for grow-out. Specialised diets may be formulated to be highly digestible or aim to provide specific nutrients at a higher level to meet nutritional requirements of the bird. The effectiveness of specifically formulated pre-starter or starter diets depends on potential carryover effect to increase the bird performance up to the market age. There is evidence that some nutrient requirements of birds, digestible amino acids in particular, in the first 10 days of life may be higher than the current industry recommendation. Among cereals, rice with its lower non-starch polysaccharide content may be a better cereal for pre-starter diets than are corn or wheat. Inclusion of fibrous ingredients in low-fibre diets of young broilers may also stimulate gut development and enhance nutrient utilisation, leading to improved bird performance. There are inconsistencies in responses to various feed additives tested specifically for pre-starter diets of broilers. Finally, with an increasing demand for removal of antibiotic growth promoters from all broiler feeds, focus on formulations to ensure sustainable growth at start will gain importance.
Publisher: Elsevier BV
Date: 04-2017
DOI: 10.3382/PS/PEW309
Abstract: The effect of dietary crude protein (CP) and additives on odor flux from meat chicken litter was investigated using 180 day-old Ross 308 male chicks randomly allocated to five dietary treatments with three replicates of 12 birds each. A 5 × 3 factorial arrangement of treatments was employed. Factors were: diet (low CP, high CP, high CP+antibiotic, high CP+probiotic, high CP+saponin) and age (15, 29, 35 days). The antibiotic used was Zn bacitracin, the probiotic was a blend of three Bacillus subtilis strains and the saponin came from a blend of Yucca and Quillaja. Odorants were collected from litter headspace with a flux hood and measured using selective ion flow tube mass spectrometry (SIFT-MS). Litter moisture, water activity (Aw), and litter headspace odorant concentrations were correlated. The results showed that low CP group produced lower flux of dimethyl amine, trimethyl amine, H2S, NH3, and phenol in litter compared to high CP group (P < 0.05). Similarly, high CP+probiotic group produced lower flux of H2S (P < 0.05) and high CP+saponin group produced lower flux of trimethylamine and phenol in litter compared to high CP group (P < 0.05). The dietary treatments tended (P = 0.065) to have higher flux of methanethiol in high CP group compared to others. There was a diet × age interaction for litter flux of diacetyl, 3-hydroxy-2-butanone (acetoin), 3-methyl-1-butanol, 3-methylbutanal, ethanethiol, propionic acid, and hexane (P < 0.05). Concentrations of diacetyl, acetoin, propionic acid, and hexane in litter were higher from low CP group compared to all other treatments on d 35 (P < 0.05) but not on d 15 and 29. A high litter moisture increased water activity (P < 0.01) and favored the emissions of methyl mercaptan, hydrogen sulfide, dimethyl sulfide, ammonia, trimethyl amine, phenol, indole, and 3-methylindole over others. Thus, the low CP diet, Bacillus subtilis based probiotic and the blend of Yucca/Quillaja saponin were effective in reducing the emissions of some key odorants from meat chicken litter.
Publisher: Elsevier BV
Date: 12-2020
Publisher: Wiley
Date: 28-09-0011
DOI: 10.1111/JPN.12414
Abstract: This study was conducted to investigate the effect of spray-dried porcine plasma (SDPP) in broiler chickens under Salmonella sofia disease challenge. The experiment comprised five starter diets: positive control (no supplement), diet supplemented with in-feed antibiotics (IFA salinomycin 0.05% + zinc bacitracin 0.033%) and diets supplemented with SDPP at 10 or 20 g/kg diet. All four of these groups were challenged with S. sofia, while a fifth group was unchallenged and used as the negative control. The experimental diets were fed to 14 days then, the birds were switched to commercial-type grower and finisher diets. Oral inoculation of the challenged groups with S. sofia occurred on day 8, 10 and 12. Body weight was significantly higher in the birds fed diets containing IFA and SDPP than in the challenged control group, but it was only significant in starter and grower phases. In general, there was an improvement in the weights of the immune-related organs, but it was only significant for the weight of the bursa of SDPP-fed birds at 13 days. At day 13, blood potassium content was lower and the concentrations of IgG and IgM tended to be lower in the birds fed on low-SDPP starter diets than those of the other groups. There were significant differences in the concentration of lactic acid in the ileum and acetic acid, formic acid, butyric acid and propionic acid in the caeca. Inclusion of SDPP to the starter diets of broiler chicks had positive effects on broiler performance, immunity and gut health during exposure to highly pathogenic conditions.
Publisher: Elsevier BV
Date: 09-2020
Publisher: Elsevier BV
Date: 11-2014
Abstract: There is generally no information regarding the influence of distillers dried grains with solubles (DDGS) on the net energy (NE) value of broiler diets. It was hypothesized that the ME content of DDGS may overestimate its productive energy, leading to higher heat increment when used in broiler diets. Furthermore, it was of interest to investigate the potential benefits of a mixture of carbohydrases and protease on energy partitioning in diets containing DDGS. In this study, 2 evaluations, comparative slaughter (CS) or indirect calorimetry (IC), were conducted using the same batch of birds and feed. Two levels of DDGS (0 or 30%) without or with an enzyme mixture were used in a 2 × 2 factorial arrangement of treatments. The CS evaluation used 240 male Ross 308 broiler chicks with 6 replicates (10 birds each) per treatment from d 18 to 28. Carcass energy values were examined in 2 birds per replicate on each of d 18 and 28 to determine heat production (HP), NE, and ME. The IC evaluation used 32 birds acclimatized to 16 closed-circuit calorimeters (2 birds per chamber) following energy partitioning measurement by gaseous exchange and total excreta collection from 18 to 24 d. Birds fed DDGS had increased feed efficiency during starter and grower phases (P < 0.001). The CS showed no difference in HP between birds fed diets with or without DDGS. When measured by IC, birds fed diets containing DDGS had higher HP and lower NE (P < 0.01). Enzyme supplementation had no effect on HP, ME, or NE:ME ratio measured using CS or IC but increased NE and NE intake (P < 0.01) from d 18 to 28. Carcass fat retention was decreased (P < 0.05) and there was a lower NE (P < 0.01) in birds fed the DDGS-diets. This study showed that 30% dietary DDGS affects energy partitioning in broilers with more heat produced, decreased dietary NE, and less fat retained in the carcass.
Publisher: Elsevier BV
Date: 05-2017
DOI: 10.3382/PS/PEW388
Abstract: A pair-feeding study was conducted to determine if reduced feed intake (FI) in broiler chickens fed high canola meal (CM) diets per se accounts for reduced growth performance and whether this lower growth rate can be mitigated by increasing dietary amino acid (AA) levels. Five experimental wheat-based diets were formulated as follows: soybean meal (SBM) diet, high CM diet with normal AA concentration, and high CM diets with 3, 6, or 9% additional AA concentration (Lys, Met+Cys, Thr, Ile, Arg, and Val). Another group of birds was pair-fed with SBM diet to the consumption levels of birds fed CM diet with normal AA. There were 6 replicates of 17 male 10-day-old Ross 308 chicks per treatment over grower and finisher periods. Birds fed the CM diets had reduced FI and BWG, but improved FCR (P < 0.01) compared to SBM ad libitum fed birds. The SBM pair-fed birds gained the same weight and exhibited similar FCR compared to CM fed birds. Additional 9% AA improved FCR (P < 0.01) compared to SBM and CM diets with normal AA. No significant differences were observed in ileal digestibility of DM, energy, crude protein, and AA between CM with normal AA and SBM diets. The additional 6 and 9% AA in CM diets increased digestibility of crude protein and some AA (P < 0.05). SBM ad libitum and CM + 6 and 9% AA fed birds had the highest and lowest relative weight of abdominal fat, respectively (P < 0.05). Addition of 6 and 9% AA in CM diets increased relative carcass and breast yields (P < 0.01). Serum triglyceride level was higher in SBM ad libitum fed birds (P < 0.05). The composition of microbiota in the ceca was not affected by treatments. This study showed that reduced growth of birds fed high CM diets is primarily mediated through reduced FI. This growth depression could partially be ameliorated by increasing dietary AA levels.
Publisher: Wiley
Date: 04-10-2021
DOI: 10.1111/FAF.12613
Abstract: Species responses to climate change are often measured at broad spatiotemporal scales, which can miss the fine‐scale changes that are most relevant to conservation and fisheries management. We develop a scaleable geostatistical approach to assess how juvenile and adult fish distributions have been shaped by changes in bottom temperature and dissolved oxygen over a recent decade of warming in the northeast Pacific. Across 38 demersal fishes, biomass trends were associated negatively with warming and positively with dissolved oxygen, but when trends in both biomass and climate were converted to velocities—the speed and direction a population would have to move to maintain consistent conditions—the effect of temperature change differed depending on local conditions. In the warmest locations, warming velocities were associated with negative biotic velocities for 19 of 69 species‐maturity combinations, and yet were almost always associated with stable or positive biotic velocities in the coolest locations (64 of 69). These spatially consistent biomass declines (negative biotic velocities) in the warmest locations and increases in cooler locations suggest a redistribution of species with the potential for new ecological and fisheries interactions. After controlling for temperature, the more spatially consistent effects of dissolved oxygen were often negative, suggesting a mechanism other than hypoxia avoidance—potentially changes in primary production. Our approach identifies the species and locations that are most sensitive to observed changes in the environment at any scale, thus facilitating future vulnerability assessments.
Publisher: JMIR Publications Inc.
Date: 23-05-2018
Abstract: ecal occult blood tests (FOBTs) are recommended by the US Preventive Services Task Force as a screening method for colorectal cancer (CRC), but they are only effective if positive results are followed by colonoscopy. Surprisingly, a large proportion of patients with a positive result do not follow this recommendation. he objective of this study was to examine the effectiveness of text messaging (short message service, SMS) in increasing adherence to colonoscopy follow-up after a positive FOBT result. his randomized controlled trial was conducted with patients who had positive CRC screening results. Randomization was stratified by residential district and socioeconomic status (SES). Subjects in the control group (n=238) received routine care that included an alert to the physician regarding the positive FOBT result. The intervention group (n=232) received routine care and 3 text messaging SMS reminders to visit their primary care physician. Adherence to colonoscopy was measured 120 days from the positive result. All patient information, including test results and colonoscopy completion, were obtained from their electronic medical records. Physicians of study patients completed an attitude survey regarding FOBT as a screening test for CRC. Intervention and control group variables (dependent and independent) were compared using chi-square test. Logistic regression was used to calculate odds ratios (ORs) and 95% CIs for performing colonoscopy within 120 days for the intervention group compared with the control group while adjusting for potential confounders including age, gender, SES, district, ethnicity, and physicians’ attitude. verall, 163 of the 232 patients in the intervention group and 112 of the 238 patients in the control group underwent colonoscopy within 120 days of the positive FOBT results (70.3% vs 47.1% OR 2.17, 95% CI 1.49-3.17 P .001) this association remained significant after adjusting for potential confounders (P=.001). text message (SMS) reminder is an effective, simple, and inexpensive method for improving adherence among patients with positive colorectal screening results. This type of intervention could also be evaluated for other types of screening tests. linicalTrials.gov NCT03642652 t2/show/NCT03642652 (Archived by WebCite at 4TlICijl)
Publisher: Informa UK Limited
Date: 02-01-2021
Publisher: Elsevier BV
Date: 03-2021
Publisher: Informa UK Limited
Date: 09-2015
DOI: 10.1080/00071668.2015.1068429
Abstract: A 4 × 2 factorial experiment was conducted to investigate the effect of inclusion of spray-dried porcine plasma (SDPP), in lieu of meat meal, in the starter diet on performance and digestive physiology of broiler chickens between hatch and 35 d of age. Four levels of SDPP (0, 5, 10 or 20 g/kg) were included in the starter diets in lieu of meat meal on either wheat- or maize-based diets. Over the first 10 d, and throughout the 35-d experimental period, birds gained more body weight with increasing concentrations of SDPP regardless to the type of grain used. Inclusion of SDPP in the starter diet markedly improved feed per gain in the starter phase and across the 35-d study. There was no significant effect of the type of grain and its interaction with SDPP on the body weight gain and feed per gain for the two assessed periods. At d 10, the relative weight of the gizzard+proventriculus, spleen and liver increased with increasing concentrations of SDPP. At 24 d of age, the grain and SDPP inclusion significantly interacted, depressing the weight of bursa and spleen in birds that received the highest concentration of SDPP in the maize-based diet. Birds fed on the maize-based diets had higher relative weight of pancreas than those on the wheat-based diets. Increasing concentrations of SDPP in the starter diet improved the activities of maltase, sucrase and alkaline phosphatase at 24 d of age. The interaction of grain and SDPP concentration was significant for sucrase activity in birds on the wheat-based diets. Chickens on maize-based diets had higher alkaline phosphatase and maltase activities than those on wheat-based diets. Chicks that were offered SDPP-containing starter diets had longer villi, deeper crypts and lower villi/crypt than the control at 24 d of age regardless of the grain type used. Furthermore, longer villi and larger villi/crypt were found in chicken groups fed on wheat-based diets than those on maize-based diets. Chickens on maize-based diets had higher dressing percentage and relative breast weight than those on wheat-based diets at 35 d of age. It can be concluded that supplementation of starter diets with SDPP would be beneficial to the long-term growth of broiler chickens. The effect of the basal grain used in the diet is minimal.
Publisher: CSIRO Publishing
Date: 2016
DOI: 10.1071/AN141021
Abstract: This study was undertaken to investigate the effect of the supplementation level and feeding duration of spray-dried porcine plasma (SDPP) on performance and digestive physiology of broiler chickens. Three inclusion levels of SDPP (0, 10 or 20 g/kg) were used in lieu of meat meal and fed from hatch to 5 or 10 days of age. At Day 10, there was an interaction (P 0.05) between the inclusion level and feeding duration of SDPP, improving bodyweight (BW) in birds that received SDPP at the highest concentration over the longer feeding duration. Inclusion of SDPP resulted in lower (P 0.01) feed intake, higher (P 0.01) BW and improved (P 0.05) feed conversion ratio (FCR). Furthermore, FCR was improved (P 0.001) in birds that were offered SDPP over the longer feeding duration than in those that received SDPP for a shorter duration. The pancreas was heavier (P 0.05) in the birds that received SDPP at the highest concentration over the long feeding duration. At 24 days of age, there was an interaction (P 0.05) between the inclusion level and feeding duration of SDPP, indicating the lowest amino acid, protein, gross energy and dry matter digestibility in the birds that received SDPP at the highest concentration over the longer feeding duration. Villus height was increased (P 0.001) by the experimental factors and their interaction, revealing longer villi in the birds that received SDPP at the low level over the short feeding duration. Apparent villus surface area was higher (P 0.001) in birds that were offered diets that contained the highest concentration of SDPP. There was an interaction (P 0.01) between experimental factors with regards to the apparent villus surface area. Up to Day 35, the level of SDPP tended to increase (P = 0.06) BW. Level (P 0.001) and feeding duration (P 0.01) improved FCR in the birds that received SDPP for the long feeding duration. It would be more economical to use a low inclusion level of SDPP over a longer period of time to achieve the same benefits.
Publisher: Elsevier BV
Date: 10-2015
DOI: 10.3382/PS/PEV219
Abstract: Resistant starch has been reported to act as a protective agent against pathogenic organisms in the gut and to encourage the proliferation of beneficial organisms. This study examined the efficacy of acetylated high amylose maize starch (SA) and butyralated high-amylose maize starch (SB) in reducing the severity of necrotic enteritis (NE) in broilers under experimental challenge. A total of 720 one-day-old male Ross 308 chicks were assigned to 48 floor pens with a 2 × 4 factorial arrangement of treatments. Factors were a) challenge: no or yes and b) feed additive: control, antibiotics (AB), SA, or SB. Birds were challenged with Eimeria and C. perfringens according to a previously reported protocol. On d 24 and 35, challenged birds had lower (P < 0.001) livability (LV), weight gain (WG), and feed intake (FI) compared to unchallenged birds. Challenged birds fed SA and SB had higher FI and WG at d 24 and 35 (P < 0.05) compared to birds fed the control diet, while being significantly lower than those fed AB. Unchallenged birds fed SA or SB had higher FI at d 24 and 35 compared to those fed the control diet (P < 0.05). Birds fed SB had increased (P < 0.001) jejunal villus height/crypt depth (VH:CD) ratios at d 15, increased ileal (P < 0.001) and caecal (P < 0.001) butyrate levels at d 15 and 24, and decreased (P < 0.01) caecal pH at d 15. Birds fed SA had increased (P < 0.001) ileal acetate content at d 24 and decreased (P < 0.01) caecal pH at d 15. These results demonstrated that dietary acylated starch improved WG in birds challenged with necrotic enteritis. Depending on the acid used, starch acylation also offers a degree of specificity in short chain fatty acid (SCFA) delivery to the lower intestinal tract which improves gut health.
Publisher: Informa UK Limited
Date: 03-03-2021
Publisher: Elsevier BV
Date: 09-2015
Publisher: Elsevier BV
Date: 03-2019
DOI: 10.3382/PS/PEY442
Abstract: Global consumption of chicken meat has increased at a faster rate than any other animal protein source, and thus refinements in energy formulation techniques for feed have continued to gain importance. Formulation of animal feed based on net energy (NE) has been implemented in ruminants and pigs but not in poultry. A closed-circuit respiratory calorimetry system was employed on 25- to 28-day-old broilers fed 19 diets formulated with varying nutrient composition to produce equations to predict NE and apparent metabolizable energy (AME) efficiency of feed for broiler chickens. Performance, energy and N balance, respiratory quotient, and energy utilization were measured in the birds. Linear regression analysis was performed to generate prediction equations for dietary energy content and AME efficiency. The NE content was positively related to AME and ether extract, but negatively to crude protein. The study generated equations that can accurately predict NE, and NE/AME using AME value and chemical composition of feeds. The NE prediction equations were further validated on a separate set of diets with high correlation (r = 0.99) and accuracy. The outcomes are an important step for the broiler industry to adapt to an NE system in place of AME systems for the formulation of broiler chicken feeds following robust validation experiments.
Publisher: Elsevier BV
Date: 05-2018
DOI: 10.1016/J.VETPAR.2018.04.009
Abstract: Reliable methods for detection of A. galli infection using excreta egg count (EEC) and ELISA assays to determine A. galli specific IgY levels in serum and yolk s les were compared from hens infected naturally and artificially. Artificially infected hens were used to generate s les for analysis of preferred detection methods and to generate contaminated ranges for use in the naturally acquired infection study in which Lohmann Brown hens (n = 200) at 16 weeks of age were randomly assigned to four treatments with five replicate pens. Hens of negative control (NC) ranged on a decontaminated area, hens of low infection, medium infection and positive control (PC) ranged on the areas previously contaminated by hens artificially infected with 250, 1000 and 2500 A. galli eggs/hen, respectively. Additionally, hens of PC were orally infected with 1000 A. galli eggs/hen. Anti A. galli antibody levels in hen serum (SIgY) and yolk (YIgY) were measured before range access, and 2, 7 and 12 weeks after access to the contaminated ranges. In a natural infection study, eggs were detected in the excreta of all hens 4 weeks after range access, with the exception of NC in which no eggs were detected. EEC increased to reach maximum value (2204 ± 307 eggs/g) after 11 weeks of range access and then declined at 12 weeks (905 ± 307eggs/g) (p < 0.01). While SIgY OD values were not different in hens between any groups before range access, after 2 weeks, both SIgY and YIgY gradually increased in hens of PC (1.17 ± 0.03 and 0.88 ± 0.04) and medium infection (1.07 ± 0.03 and 0.96 ± 0.04) compared to low infection (0.38 ± 0.03 and 0.29 ± 0.04) (p < 0.01) and NC. After 12 weeks, SIgY were similar in hens of PC, medium and low groups whereas YIgY was higher in hens of low infection group (p < 0.01). Sensitivity of the serum and egg yolk antibody levels assay to detect A. galli infection was 100% and 96%, respectively, whereas the pooled EEC method yielded a sensitivity of 93%. The results of this study suggest that hens naturally infected with A. galli produce both SIgY and YIgY at different levels depending on the infection intensity and duration of exposure which allows the diagnosis of prior infection or early diagnosis of current infection. Use of the practical and non-invasive method of yolk s le analysis for detecting IgY can be just as informative as using serum s les to detect A. galli infection.
Publisher: Public Library of Science (PLoS)
Date: 28-08-2014
Publisher: Elsevier BV
Date: 03-2015
Publisher: Elsevier BV
Date: 03-2017
Publisher: Elsevier BV
Date: 03-2015
Publisher: Informa UK Limited
Date: 20-06-2017
DOI: 10.1080/03079457.2017.1330536
Abstract: Broilers commonly suffer from necrotic enteritis (NE). Other gastrointestinal infectious diseases affect poultry, including nematode infections which are considered a re-emerging disease in barn and free-range systems. The aim of this study was to characterize the immune response of broilers after artificial infection with NE and contrast these with responses to the nematode Ascaridia galli and determine whether immune parameters measured during the course of infection can be used to distinguish infected from uninfected birds. A total of 96 one-day-old male Ross 308 broiler chickens were used in this study. At 10 days of age, broilers were randomly assigned to one of the following treatment groups: control birds (n = 32), A. galli infected birds (n = 32), or NE infected birds (n = 32) and inoculated with the appropriate infective agents. The immune response of birds was monitored through evaluation of haematology parameters, acute phase protein production, and intraepithelial intestinal lymphocyte population changes at 11, 16, 20, and 32 days of age. T-helper cells (CD4
Publisher: CSIRO Publishing
Date: 04-03-2022
DOI: 10.1071/AN21393
Abstract: Context Improving immune status through nutritional adjustments may be part of an effective strategy to reduce reliance on antibiotic growth promoters for controlling necrotic enteritis (NE) in broiler chickens. Aims This study examined the effect of dietary protein level and the replacement of crystalline l-arginine (Arg) with l-citrulline (Cit) in the reduced-protein diet on the performance of broilers challenged with subclinical NE. Methods Ross 308 cockerels (n = 720) were randomly allocated to six dietary treatments, with eight replicates of 15 birds per pen, during a 35-day feeding experiment. The treatments were as follows: standard protein without NE challenge (SP−) standard protein with NE challenge (SP+) reduced protein (two percentage points lower crude protein) without NE challenge (RP−) reduced protein with NE challenge (RP+) RP+ plus added Arg (103% of RP, RPA+) and RPC+ where supplemental Arg in RPA+ was replaced with Cit. The first four treatments were considered as a 2 × 2 factorial arrangement, with factors being NE (− or +) and protein level (SP or RP). Treatments SP+, RP+, RPA+, and RPC+ were analysed by one-way ANOVA. Key results Subclinical NE challenge reduced feed intake (FI), reduced body weight gain (BWG) and increased feed to gain ratio (FCR) from Day 0 to Day 35, increased intestinal lesion scores on Day 16, and reduced relative breast yield on Day 35 (P 0.05). Feeding RP diets increased FI (P 0.001), increased BWG (P 0.01) and reduced FCR (P 0.01) during the grower phase compared with SP diets when birds were challenged with NE. Birds in the RPC+ treatment had a lower overall FCR than did those in the SP+ treatment (P 0.001). Birds in the RPA+ treatment had similar FI, BWG and FCR to those in the RP+ treatment (P 0.05). Conclusions Collectively, the results showed protective effects of replacing the supplemental Arg with Cit against NE in RP diets, as indicated by higher performance during and after the challenge. Implications Feeding the RP diets supplemented with Cit may be part of an effective strategy to reduce reliance on antibiotic growth promoters for controlling NE in broiler chickens.
Publisher: CSIRO Publishing
Date: 02-05-2022
DOI: 10.1071/AN21394
Abstract: Context Necrotic enteritis (NE) has been considered a major threat to broiler gut health and growth performance. Aims This study aimed at investigating the effects of l-arginine (Arg) or l-citrulline (Cit) supplementation on intestinal morphology, short-chain fatty acid (SCFA), microbiota count, gut permeability, and pH in broilers fed reduced-protein diets during subclinical NE challenge. Methods Ross 308 cockerels (n = 720) were randomly assigned to six experimental treatments with eight replicates of 15 birds per pen. The treatments were standard protein without NE challenge (SP−), or with NE challenge (SP+) reduced protein (two percentage points lower crude protein) without NE challenge (RP−), or with NE challenge (RP+) RP+ plus added Arg (103% of Ross 308 requirement, RPA+) and RPC+ where supplemental Arg in RPA+ was replaced with Cit. A 2 × 2 factorial arrangement was employed for the first four treatments. Factors were NE (− or +) and protein concentration (SP or RP). Treatments SP+, RP+, RPA+, and RPC+ were analysed by one-way ANOVA. Key results Necrotic enteritis × protein interactions were detected for serum fluorescein isothiocyanate dextran (FITC-d) level, C. perfringens (P 0.05) count in the caeca (P 0.01), and acetic acid (P 0.01) and total SCFA concentrations in the ileum on Day 16 (P 0.001). Feeding the RP diet reduced serum FITC-d concentration, number of C. perfringens in the caeca, and increased acetic acid and total SCFA concentrations in the ileum compared with the SP group only in birds challenged with NE. Birds in the RPC+ treatment had greater jejunal villus height (P 0.001), and lower caecal C. perfringens and Enterobacteriaceae count than did those in the SP+ treatment (P ≤ 0.001). Conclusions The results indicated a benefit to gut health of broilers during NE challenge when replacing crystalline Arg with Cit in RP diets. Implications In part, replacement of Arg by Cit in the RP diets is of great potential to increase gut health, reduce growth loss, thus, minimising negative effects of NE in broilers.
Publisher: Elsevier BV
Date: 12-2016
Publisher: CSIRO Publishing
Date: 07-2022
DOI: 10.1071/AN21395
Abstract: Context The impact of necrotic enteritis (NE) on acute-phase proteins, interleukins, blood mineral profiles, and gene expression have not been well documented. Aims This study aimed to determine the effects of l-arginine (Arg) or l-citrulline (Cit) supplementation on serum immunological parameters, serum mineral composition and gene expression in broilers fed reduced-protein diets (RP) during subclinical NE challenge. Methods Ross 308 cockerels (n = 720) were randomly assigned to six experimental treatments, with eight replicates of 15 birds per pen. The treatments were standard protein without and with NE challenge (SP−, SP+) reduced protein (2% points lower crude protein) without and with NE challenge (RP−, RP+), RP plus added Arg (103% of Ross 308 requirement) with NE challenge (RPA+) and RPC+ where supplemental Arg in RPA+ was replaced with Cit. A 2 × 2 factorial arrangement was employed for the first four treatments. Additionally, treatments SP+, RP+, RPA+, and RPC+ were analysed by one-way ANOVA. Key results The NE × protein interactions indicated that serum calcium concentration decreased in birds fed the RP diets only when challenged with NE (P 0.05). The NE × protein interactions showed that the NE challenge downregulated the mRNA expression of jejunal y+ L amino acid transporter-2, and mucin 2 only in birds fed the RP diets (P 0.05). Feeding the RP decreased expression of catenin alpha 1, but increased expression of claudin 5 and tight junction protein genes compared with the SP (P 0.05). Birds in the RPC+ treatment had increased gene expression of tight junction protein and claudin 5 compared with the SP+ treatment (P 0.05). Conclusions Dietary protein level and infection with NE both have an impact on immune response and expression of genes involved in immunity and nutrient digestibility. In part replacement of Arg with Cit in the RPC diet may have beneficial effects on gene expression in NE-challenged birds. Implications Feeding RP diets may alleviate a decline in growth during subclinical NE by increasing gene expression of tight junction proteins compared with the SP diets.
Publisher: Informa UK Limited
Date: 06-2018
Publisher: Elsevier BV
Date: 11-2017
DOI: 10.3382/PS/PEX225
Abstract: An experiment was conducted to evaluate the effects of sugarcane bagasse (SB) and particle size on broiler performance, gizzard development, ileal microflora, litter quality, and bird welfare under a wet litter challenge model. A total of 672 one-day-old Ross 308 male broilers was allocated to 48 pens using a 2 × 2 × 2 factorial arrangement of treatments with corn particle size-coarse 3,576 μm (CC) or fine 1,113 μm (FC) geometric mean diameter, SB - 0 or 2% and sodium (Na) - 0.16 or 0.40% with increased Na level to induce wet litter. A 3-way particle size × Na × SB interaction (P < 0.05) was observed for weight gain at d 10. Birds fed FC showed a higher weight gain compared to birds fed CC when 0.40% Na without SB diet or 0.16% Na with 2% SB diet was offered. A significant particle size × SB interaction was observed at d 24 on feed conversion ratio (FCR P < 0.001) and weight gain (P < 0.05). FCR was reduced by 2% SB supplementation in birds fed CC but increased in birds fed FC. Further, weight gain of birds fed 2% SB was higher in birds fed CC but not in those fed FC. On d 35, birds fed 2% SB had a higher weight gain (P < 0.001) compared to those without SB, and a SB × particle size interaction on relative gizzard weight (P < 0.05) and pH (P < 0.05) was present. SB reduced gizzard pH and increased the relative gizzard weight in birds fed the FC diet but not the CC diet (P < 0.05). Counts of ileal Bacillus spp. were increased in birds fed SB (P < 0.05) on d 24. No effects of SB and particle size on litter quality and bird welfare were observed, but higher Na increased litter moisture and footpad dermatitis (FPD) scores (P < 0.001). These findings suggest that SB independently or in combination with CC improves performance in older birds regardless of Na level in diets, possibly through improved gizzard development and gut microflora of birds.
Publisher: Wiley
Date: 07-01-2021
DOI: 10.1111/JPN.13486
Abstract: Arginine activity in broiler diets can be supplied by L‐arginine (Arg), guanidinoacetic acid (GAA) and L‐citrulline (Cit), all of which are commercially available. This study was conducted to assess the effects of Arg source and level on broiler performance, nutrient digestibility and carcass parameters. Day‐old Ross 308 cockerels ( n = 768) were assigned to one of eight dietary treatments using a completely randomized design: normal protein (NP), low protein deficient in Arg (LP) and LP with two levels of either Arg (0.238% and 0.476%), GAA (0.309% and 0.618%) or Cit (0.238 and 0.476%). The LP was 5 percentage points lower in protein level than the NP. Wheat, sorghum, soya bean meal, canola meal, and meat and bone meal‐based diets were fed over three feeding phases to 6 replicate floor pens with 16 birds each. Compared to NP, birds fed LP had reduced feed intake (FI, p 0.001), reduced body weight gain (BWG, p 0.001) and increased feed conversion ratio (FCR, p 0.001) from day 0 to day 35. Additions of Arg or Cit to the LP at both levels resulted in increased BWG and reduced FCR ( p 0.05). Birds fed LP with GAA added had lower FCR ( p 0.05) but not higher BWG ( p 0.05) compared with the LP observed from day 0 to day 35. Supplementation of Arg, Cit and the low level of GAA to LP resulted in increased carcass yield, bone length, diameter and ash ( p 0.05) but did not increase ileal energy or nitrogen digestibility ( p 0.05). The findings indicate that Cit is an efficacious source of Arg activity in Arg‐deficient diets.
Publisher: Elsevier BV
Date: 03-2019
DOI: 10.3382/PS/PEY479
Abstract: Pasture intake can be a major challenge for free-ranging hens. This study was conducted to examine pasture digestion and to manage its negative effects. A total of 300 ISA Brown laying hens were used to investigate the effect of time on range (T) in short-term (6 wk) and long-term (12 wk) of 2 range types (R) (gravel vs. pasture) and dietary supplements (F) (T1 = xylanase T2 = xylanase/beta-glucanase ectinase rotease T3 = xylanase/benzoic acid/essential oils) on crude protein, crude fiber, acid detergent fiber, neutral detergent fiber, non-starch polysaccharides (NSP), calcium and phosphorus digestibility, pH of the crop, and ileum digesta viscosity and morphology. Hens exposed to the range for 12 wk had lower (P < 0.05) digestibility of crude protein, insoluble rhamnose, ribose, and lower ileal pH compared to hens that ranged for 6 wk. Hens ranging on pasture had lower digestibility (P < 0.05) of crude protein, acid detergent fiber, neutral detergent fiber, insoluble arabinose, and insoluble xylose, but higher digestibility (P < 0.05) of insoluble mannose and glucose compared to hens that ranged on gravel. Hens fed T2 and T3 had higher digestibility (P < 0.05) of CP, acid detergent fiber, and neutral detergent fiber compared to hens fed T1. Hens fed T2 had higher digestibility (P < 0.05) of free oligosaccharide arabinose and xylose than those fed T1 or T3 diets. A significant interaction between T × R was detected for crude fiber digestibility and villus height. Digestibility of crude fiber was reduced and villus height was increased in hens ranged on pasture for 12 wk compared to 6 wk. An interaction between R × F was observed on phosphorus and soluble NSP digestibility (P < 0.05). Hens fed T2 and T3 diets had lower digestibility of phosphorus and NSP on gravel than on pasture. In conclusion, pasture consumption impaired the digestibility of nutrients. Supplementing free-range diets with a multi-enzyme or xylanase/benzoic acid/essential oil product reduced these negative effects and increased the ileal nutrient digestibility.
Publisher: Informa UK Limited
Date: 06-2017
Publisher: Elsevier BV
Date: 02-2017
DOI: 10.3382/PS/PEX347
Abstract: This study was conducted to determine the effect of Ascaridia galli infection on free-range laying hens. Lohmann Brown laying hens (n = 200) at 17 wk of age were allocated to 4 treatment groups (n = 50 per group), each with 5 replicate pens of 10 hens. Hens in 3 treatment groups were orally inoculated with different doses of embryonated A. galli eggs: low (250 eggs), medium (1,000 eggs), and high (2,500 eggs) levels, whereas hens of the control group were not infected. Infection levels were monitored using excreta egg counts and mature A. galli worm counts in the intestine. Anti A. galli antibody titers (IgY) in the serum were measured prior to infection, and at 6, 11, 15, and 20 wk post infection (PI) and in egg yolk at 11 and 20 wk PI. Parameters evaluated included feed intake, egg production, egg weight, egg mass, FCR, liver weight, liver fat, and intra epithelial immune cell infiltration. The results showed no difference in feed intake, body weight, or FCR among any treatment groups (P > 0.05). Egg production was lower in the low infection group compared to other groups at 20 wk of age (P < 0.01). Serum IgY was higher in the infected groups' hens at 20 wk PI compared to control group hens (P < 0.01). Yolk IgY increased significantly over time and was higher in infected hens compared to hens of the control group at 11 and 20 wk PI (P < 0.001). No differences were observed in liver lipid content or intraepithelial lymphocytes infiltration among treatment groups. Ascaridia galli eggs in the coprodeum content and adult A. galli worm count were higher in infected hens compared to hens of the control group (P < 0.01). In conclusion, the effects of artificial infection with A. galli on the parameters investigated were minor, and egg yolk antibody may be a more reliable indicator of A. galli infection than serum antibody or excreta egg count.
Publisher: Elsevier BV
Date: 03-2018
Publisher: Informa UK Limited
Date: 03-2018
Publisher: Elsevier BV
Date: 06-2021
Publisher: Unpublished
Date: 2015
Publisher: CSIRO Publishing
Date: 17-02-2022
DOI: 10.1071/AN21539
Abstract: Context Mineralisation of the bone matrix is a pivotal factor affecting bone strength in broilers. Reduced bone strength might cause fracture during catching and transportation to slaughterhouses, leading to economic loss. Aims This study was conducted to investigate the effects of l-arginine (Arg), guanidinoacetic acid (GAA), and l-citrulline (Cit) supplementation to Arg-deficient reduced-protein diets on bone mineral composition and mineral digestibility of broiler chickens. Methods Day-old Ross 308 males were allocated to one of eight dietary treatments with six replicates of 16 birds per treatment. The treatments were standard protein (SP), reduced protein deficient in Arg (RP), and RP with two levels of either Arg (0.238% and 0.476%), GAA (0.309% and 0.618%) or Cit (0.238% and 0.476%). The difference in crude protein content between RP and SP was five percentage points. Key results Birds fed the RP diet had higher ileal digestibility of P, Na, Mg, S, Al, Fe, Cu and Zn than did those fed the SP on Day 23 (P 0.01). Supplementation of both Arg, GAA and Cit to the RP reduced Mg digestibility on Day 23 (P 0.001). The tibia and femur Ca and P concentrations were not different among the dietary treatments on either Day 23 or Day 35 (P 0.05). Birds fed the RP had lower tibia and femur B concentrations and higher tibia and femur Zn concentrations on Day 23 and Day 35 and higher tibia and femur Mn concentrations on Day 35 than did those fed the SP (P 0.01). Supplementation of Arg at the high level and Cit at both levels to the RP increased tibia S concentration on Day 23 (P 0.001). Supplementation of Arg, Cit and GAA to the RP decreased femur Zn concentration on Day 35 (P 0.001). Supplementation of GAA at the high level decreased concentrations of the tibia and femur B and Fe on Day 23, but increased tibia and femur Mn concentrations on Day 23 and Day 35 (P 0.01). Conclusions The findings indicated an increased mineral digestibility but relatively similar bone mineral concentrations in broilers fed the RP diets when compared with the SP diets. Implications The results of the current study may provide useful information on the bird bone mineralisation and help expand the adoption of reduced-protein diets in the poultry industry.
Publisher: Elsevier BV
Date: 06-2021
Publisher: Elsevier BV
Date: 09-2018
DOI: 10.3382/PS/PEY197
Abstract: This experiment was conducted to examine the effect of trace mineral sources on broiler performance, carcass composition, trace mineral digestibility, and tibia bone quality of broiler chickens. A total of 480 Ross 308 male day-old chicks were allocated to 24 pens and assigned to 4 dietary treatments in a completely randomized design. Treatments were as follows: inorganic (I) was basal diet supplemented with 750 g/t inorganic trace mineral premix organic 1 (O1) and organic 2 (O2) was basal diet supplemented with 375 and 500 g/t organic yeast proteinate trace mineral premix respectively and hydroxychloride (H) was basal diet supplemented with 1000 g/t salt encrusted trace mineral premix. On day 25, no differences in feed intake (FI), body weight gain (BWG), feed conversion ratio (FCR), or livability (LV) were observed between treatments (P > 0.05). On day 38 birds fed O1 and H had higher weight gain (P < 0.05) and lower FCR (P 0.05) on day 38. Spleen percentage of body weight on day 25 was increased in birds fed O1 and H treatments (P 0.05). Apparent digestibilities of Cu and Zn were greater in birds fed yeast proteinated trace minerals compared to other sources.
Publisher: JMIR Publications Inc.
Date: 05-02-2019
DOI: 10.2196/11114
Publisher: Elsevier BV
Date: 05-2015
DOI: 10.3382/PS/PEV047
Abstract: Six expeller-extracted canola meal (ECM) s les produced under different seed conditioning temperatures (90, 95, or 100°C) and screw torques in the second press (low or high) were evaluated in a 3 × 2 factorial arrangement of treatments to determine the effect of processing on standardized ileal amino acid digestibility (SID) in 10 and 24-day-old broilers. A nitrogen-free diet was fed to determine ileal endogenous amino acid flow. Each diet was fed for 5 d to 6 replicate cages of 10 or 7 chicks, and ileal s les were collected at 10 and 24 d, respectively. The endogenous flow (mg/kg DM intake) of CP and all amino acids except Cys decreased (P < 0.01) with age. Conditioning temperature by screw torque interactions were detected (P 0.05). Meals processed under medium conditioning temperature (95°C) at either low or high screw torque had the greatest (P < 0.05) AID of CP and total amino acids. The SID values followed a similar pattern as AID at both ages. When corrected for endogenous losses, the average AID of total amino acids improved approximately 3.5 and 2.0 units, at 10 and 24 d, respectively. The AID and SID values increased (P < 0.05) with age for most AA, but the effect of age was not consistent between ECMs. A negative correlation was detected between NDF and neutral detergent-insoluble nitrogen (NDIN) content of the meals and SID values of Lys at d 24 (r = -0.79, r = -0.76 P = 0.001, respectively). Processing conditions affected CP and amino acid digestibility, likely because of alterations to the chemical composition of ECM and formation of indigestible complexes of amino acids with fiber. The AID and SID values increased with age independent of meal processing conditions.
Publisher: Elsevier BV
Date: 09-2014
Abstract: The objective of this study was to investigate the effect of processing conditions and chemical composition on ileal digestible energy (IDE), AME, and AMEn of 6 expeller-extracted canola meal (ECM) s les subjected to conditioning temperature at 90, 95, or 100°C and high or low screw torque over the second presses in a 3 × 2 factorial arrangement. The ECM s les were incorporated into a corn-soybean meal reference diet at 30% by replacing energy-yielding ingredients. A total of 210 one-day-old male broiler chicks (Ross 308) were fed common starter and grower diets until d 18, and then assigned to 7 experimental diets replicated 6 times, with 5 chicks per cage. After a 5-d diet acclimation period from d 18 to 22, excreta was collected for 72 h. The difference method was used to determine AME, which was corrected to zero N balance to obtain AMEn. Medium seed conditioning temperature resulted in the highest IDE, AME, and AMEn compared with low or high temperature, and high screw torque resulted in higher energy utilization compared with low torque (P < 0.001). There was also an interaction (P < 0.001) between conditioning temperature and screw torque. For ECM subjected to low or medium conditioning temperature at low screw torque, IDE, AME, and AMEn values ranging from 2,137 to 2,705, 2,089 to 2,655, and 1,977 to 2,482 kcal/kg of DM, respectively, were obtained. The mean AMEn values were 2,260 kcal/kg of DM, indicating a 7% reduction compared with AME values. The AMEn values were negatively correlated with neutral detergent fiber (NDF r = -0.93 P = 0.001) and NDIN (r = -0.87 P = 0.001). Stepwise regression to predict AMEn value resulted in the following equation: AMEn (kcal/kg of DM) = 3,397.8 + (-100.1 × NDF %) + (279.5 × ash %) + (-33.8 × ADF %) (R² = 0.91 SE = 61.9 P = 0.001). These results indicate that AMEn values vary markedly among ECM s les, and chemical constituents, especially the fiber components, may have a considerable effect on AMEn value.
Publisher: Springer Science and Business Media LLC
Date: 11-02-2021
DOI: 10.1186/S13584-021-00444-2
Abstract: Colorectal cancer leads to significant morbidity and mortality. Early detection and treatment are essential. Screening using fecal occult blood tests has increased significantly, but adherence to colonoscopy follow-up is suboptimal, increasing CRC mortality risk. The aim of this study was to identify barriers to colonoscopy following a positive FOBT at the level of the patient, physician, organization and policymakers. This mixed methods study was conducted at two health care organizations in Israel. The study included retrospective analyses of 45,281 50–74 year-old members with positive fecal immunochemical tests from 2010 to 2014, and a survey of 772 patients with a positive test during 2015, with and without follow-up. The qualitative part of the study included focus groups with primary physicians and gastroenterologists and in-depth interviews with opinion leaders in healthcare. Patient lack of comprehension regarding the test was the strongest predictor of non-adherence to follow-up. Older age, Arab ethnicity, and lower socio economic status significantly reduced adherence. We found no correlation with gender, marital status, patient activation, waiting time for appointments or distance from gastroenterology clinics. Primary care physicians underestimate non-adherence rates. They feel responsible for patient follow-up, but express lack of time and skills that will allow them to ensure adherence among their patients. Gastroenterologists do not consider fecal occult blood an effective tool for CRC detection, and believe that all patients should undergo colonoscopy. Opinion leaders in the healthcare field do not prioritize the issue of follow-up after a positive screening test for colorectal cancer, although they understand the importance. We identified important barriers that need to be addressed to improve the effectiveness of the screening program. Targeted interventions for populations at risk for non-adherence, specifically for those with low literacy levels, and better explanation of the need for follow-up as a routine need to be set in place. Lack of agreement between screening recommendations and gastroenterologist opinion, and lack of awareness among healthcare authority figures negatively impact the screening program need to be addressed at the organizational and national level. This study was approved by the IRB in both participating organizations (Meuhedet Health Care Institutional Review Board #02–2–5-15, Maccabi Healthcare Institutional Review Board BBI-0025-16). Participant consent was waived by both IRB’s.
Publisher: Elsevier BV
Date: 06-2020
Publisher: CSIRO Publishing
Date: 2013
DOI: 10.1071/AN13204
Abstract: A study was conducted to determine the predictability of energy balance and energy efficiency by using dietary chemical composition. Closed-circuit indirect calorimetry was used to determine the apparent metabolisable energy (AME), respiratory quotient, heat increment (HI), net energy (NE) and ratio of NE to AME (NE : AME) of a series of diets with varying levels of chemical constituents. Diets were analysed for DM, gross energy, protein, fat, ash, crude fibre, acid detergent fibre, neutral detergent fibre, starch, sugars (mono- and disaccharides), and soluble, insoluble and total non-starch polysaccharides. Ross 308 male broilers were acclimatised to chambers and diets for 3 days and 12 days, respectively, before O2 consumption and CO2 expiration were measured gravimetrically. Gross energy of feed consumed and excreta voided were measured and AME was calculated. Heat production was calculated using the Brouwer equation based on O2 and CO2. After taking fasting heat production into account by using a value of 450 kJ/BW0.70, HI was determined. NE was calculated as AME minus HI. The results showed high predictability of AME (R2 = 0.89) and NE (R2 = 0.85) by using chemical components. HI was less predictable (R2 = 0.25). Efficiency of energy utilisation (NE : AME) was predicted (R2 = 0.40). Closed-circuit calorimetry was found to be useful for evaluating the contribution of the chemical components of feed ingredients to the efficiency of energy utilisation in broilers. These results may be used to reduce energy costs in broiler feed formulation.
Publisher: Informa UK Limited
Date: 09-06-2017
DOI: 10.1080/00071668.2017.1327705
Abstract: 1. This study investigated the prebiotic properties of arabinoxylo-oligosaccharides (AXOS) produced both in situ and in vitro for their activity against the onset of necrotic enteritis in broiler chickens. 2. A 2 × 3 factorial arrangement was applied, including necrotic enteritis challenge (challenged/unchallenged) and three dietary treatments from d 10 to 21. A wheat-soy commercial-type basal-grower diet was fed with 2% of the wheat proportion replaced by the same amount of either arabinoxylan (AX), AXOS produced from hydrolysing AX with 16 000 BXU (birch xylanase unit) xylanase in vitro or AX fed with 16 000 BXU xylanase (AX + E). Necrotic enteritis (NE) challenge was induced by orally infecting birds with a vaccine strain of Eimeria oocysts at d 9 of age followed by oral gavage of a freshly prepared Clostridium perfringens broth at d 14. 3. The challenge depressed growth performance, induced gross lesions and reduced ileal viscosity at d 10-21. Birds fed on the AXOS diet had numerically less severe gross lesions, improved feed conversion at d 0-16 and lower ileal viscosity at d 16 compared to birds fed on AX. Weight gain of the unchallenged birds ranked as follows in terms of the diets: AXOS > AX + E > AX. AX + E produced a lower ileal viscosity compared to the AX treatment but only led to marginal improvements in performance and intestinal lesion scores. 4. Caecal short-chain fatty acid (SCFA) concentration was higher in birds fed on AXOS and AX + E compared to those fed on AX and was higher in the challenged birds compared to the unchallenged birds. Gizzard pH was lower in birds fed on AX + E compared to those fed on AXOS at d 16. Challenged birds had lower ileum pH compared to the unchallenged birds at d 16 and 21. 5. Results of this study suggest that AXOS appeared to be efficacious prebiotics, as highlighted by improvements in feed conversion ratio and increased SCFA production. Future studies are warranted to elucidate the types of AXOS that are most active against NE and the mechanisms by which different levels of AXOS enhance bird performance.
Publisher: American Association of Avian Pathologists (AAAP)
Date: 03-2016
Publisher: SAGE Publications
Date: 2019
Abstract: Fecal occult blood tests are recommended for colorectal cancer screening, but are only effective if colonoscopy follows positive results. Patients with positive results often do not complete follow-up. This study examined the association between patient comprehension and adherence to colonoscopy after positive FIT (Fecal Immunochemical Test). Five hundred twenty-two patients completed a telephone questionnaire regarding the FIT and its implications 120 days after a positive result. Patients were asked whether they had the test, received the results, and required follow-up. These questions were used to identify the degree to which patients understood medical information. A participant who answered “no” to any question was defined as having “low comprehension” regarding the FIT, and participants who answered “yes” to all 3 questions, as having “high comprehension”. Comprehension and colonoscopy adherence were significantly associated. Adherence to colonoscopy was significantly higher among participants with high comprehension, after adjusting for gender, age, education, ethnicity, and socio-economic status. This study demonstrates a link between health comprehension and patient follow-up after positive FIT and contributes to understanding the implications of health comprehension in terms of health promotion. We recommend patients undergoing screening tests receive clear explanations regarding need for follow-up of positive results thus reducing health disparities associated with health comprehension.
Publisher: Elsevier BV
Date: 12-2017
Publisher: American Association of Avian Pathologists (AAAP)
Date: 03-2015
DOI: 10.1637/10774-011614-REG.1
Abstract: Necrotic enteritis (NE) is an important infectious disease in chickens. Predisposing factors play critical roles both in disease outbreaks in the field and in models for experimental induction of disease. Systematic manipulation and study of predisposing factors help to optimize methods for the experimental reproduction of disease. The nature of such factors may play a confounding role in challenge models and, therefore, warrant investigation to determine their importance in industry-relevant NE reproduction models. In the present study, we examined the roles of dietary fishmeal inclusion, Eimeria inoculation (E), and Clostridium perfringens challenge (C) on broiler growth performance and induction of NE infection. The results showed that E, preceding C, greatly increased the severity of NE induced in broiler chickens, but fishmeal addition played only a marginal role in the challenge model. Bird performance was significantly affected by all three factors during the 35-day experimental period. Fishmeal increased body weight, but statistically significant effects of fishmeal were not observed on feed conversion ratio (FCR) and feed intake. Both Eimeria and C. perfringens significantly reduced body weight gain and feed intake. E but not C led to significantly poorer FCR. These findings indicate that dietary fishmeal may be removed from the model to allow the performance results of challenged chicks to be equivalent to the performance of chicks in the field. In conclusion, the present study demonstrates that an NE challenge model without fishmeal is valid and removes bird performance bias in the model introduced by feeding high fishmeal diets, refining the model to facilitate the yield of more commercially relevant results.
Location: Israel
Location: Australia
No related grants have been discovered for Robert Swick.