ORCID Profile
0000-0002-0913-5409
Current Organisations
University of Nottingham Malaysia
,
University of Nottingham
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Publisher: Elsevier BV
Date: 04-2022
Publisher: Elsevier BV
Date: 11-2022
DOI: 10.1016/J.BIORTECH.2022.128085
Abstract: Microalgae are known for containing high value compounds and its significant role in sequestering carbon dioxide. This review mainly focuses on the emerging microalgae cultivation technologies such as nanomaterials technology that can improve light distribution during microalgae cultivation, attached cultivation and co-cultivation approaches that can improve growth and proliferation of algal cells, biomass yield and lipid accumulation in microalgal. This review includes a comprehensive discussion on the use of microbubbles technology to enhance aerated bubble capacity in photobioreactor to improve microalgal growth. This is followed by discussion on the role of microalgae as phycoremediation agent in removal of contaminants from wastewater, leading to better water quality and high productivity of shellfish. The review also includes techno-economic assessment of microalgae biorefinery technology, which is useful for scaling up the microalgal biofuel production system or integrated microalgae-shellfish cultivation system to support circular economy.
Publisher: Frontiers Media SA
Date: 16-02-2021
DOI: 10.3389/FENRG.2020.637846
Abstract: Banana peels waste can be utilized to produce high quality biochar that can be incorporated into the soil for sustainable production of crops. This research analyzed several properties of the biochar produced from the banana peel at different temperatures, residence times and heating rates. This study focuses on the biochar yield and the EDX analysis of the biochar produced. Response surface methodology using central composite design (CCD) was used to optimize these parameters in the batch reactor pyrolysis system. These factors were operated in different ranges for banana peels, in which pyrolysis temperature (200 to 600°C), residence time (60 to 180 min) and heating rate (5 to 15°C·min −1 ) were varied using 20 experiments respectively. Quality of the biochar was determined based on the biochar yield and O/C ratio. The optimum biochar chosen from the CCD model was applied to several pots of Ipomoea aquatica in different biochar dosage levels of 0, 3, 9 and 15 g (0, 1, 3 and 5 wt% of soil) respectively. Pot experiment was conducted with completely randomized design (CRD) of one factor with five replications to correlate the average plant heights with the biochar dosage levels. Results showed that biochar dosage of 1% yields the highest average final Ipomoea aquatica plant height of 37.04 cm.
Publisher: MDPI AG
Date: 31-10-2018
DOI: 10.3390/SU10113960
Abstract: The rapid emergence of Industry 4.0 implies that our engineering graduates need to acquire new competences to adapt to the digital transformation. This paper evaluates the benefits of integrating 3D printing and Industry 4.0 into engineering undergraduate programs. Surveys were conducted to gather the feedbacks and views from academics and students. 75% and 86% of the participating students and lecturers, respectively, have heard about Industry 4.0. 63% of the students were exposed to modules with such elements. Tangible 3D-printed models enable visualization of fundamental theories and concepts. Enhanced 3D drawing skills and rapid 3D-printed prototypes can greatly help students study common processing equipment, manufacturing, maintenance, logistics, and operations. Some limitations were identified such as budgeting, lack of knowledge, and difficulty in changing from traditional pedagogy. This paper thus proposes a blended learning model for integrating Industry 4.0 into engineering teaching, which consists of traditional, online learning, and flipped classroom approaches. Implementation of the model can be started off with cross-multidisciplinary collaborations or expert-led training for the instructors, followed by traditional face-to-face teaching and online learning. Flipped classroom is one of the essential components of the model which encourages learning-by-making approaches such as ‘bring your own device’ and ‘do it yourself’. Integrating Industry 4.0 into engineering teaching can create a student-based learning environment, where students are gradually trained to become proactive and lifelong learners who are more conscious of the environment and economy.
Publisher: MDPI AG
Date: 09-11-2021
Abstract: In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural waste using conventional and advanced techniques of extraction and purification via a liquid biphasic system (LBS). In comparison to other methods, such as membrane extraction, precipitation, ultrasonication, and chromatography, the LBS stands out as an efficient, cost-effective, and adaptable developing method for protein recovery. The two-phase separation method can be water-soluble polymers, or polymer and salt, or alcohol and salt, which is a simpler and lower-cost method that can be used at a larger purification scale. The comparison of different approaches in LBS for amylase purification from agricultural waste is also included. Current technology has evolved from a simple LBS into microwave-assisted LBS, liquid biphasic flotation (LBF), thermoseparation (TMP), three-phase partitioning (TPP), ultrasound-assisted LBS, and electrically assisted LBS. pH, time, temperature, and concentration are some of the significant research parameters considered in the review of advanced techniques.
Publisher: Elsevier BV
Date: 08-2021
Publisher: Elsevier BV
Date: 12-2021
Publisher: Elsevier BV
Date: 04-2022
DOI: 10.1016/J.ENVRES.2021.112458
Abstract: This study explores the role of renewable energy (RE) penetration in Malaysia's energy security (ES) and its implications for the country's target of 20% capacity in the energy mix by 2025. Renewable energy (RE) is a critical driver of long-term energy security. In 2018, the share of renewable energy in Malaysia's energy mix was 9%, falling far short of the national target of 20% penetration by 2025. This study employs a system dynamics approach to investigate the relationship between RE penetration and correlated indicators from energy security (ES) dimensions: energy availability, environmental sustainability, and socio-economics. The causal relationships between the three-dimensional indicators of ES have been established using causal and stock and flow logic. Simulated results show that energy consumption has increased sharply, while energy efficiency and economic growth have only increased by a small margin with an increase in RE from 2015 to 2020. The energy intensity is expected to rise slightly by the end of the fifth year. As a result, the overall impact is positive for Malaysia's environmental sustainability while reducing its reliance on energy imports and meeting national economic growth demands.
Publisher: Elsevier BV
Date: 07-2021
Publisher: Elsevier BV
Date: 10-2021
Publisher: Elsevier BV
Date: 10-2022
Publisher: Elsevier BV
Date: 07-2021
Publisher: MDPI AG
Date: 28-04-2020
DOI: 10.3390/PR8050523
Abstract: The purpose of this review is to reveal the lipid and protein contents in black soldier fly larvae (BSFL) for the sustainable production of protein and energy sources. It has been observed from studies in the literature that the larval lipid and protein contents vary with the rearing conditions as well as the downstream processing employed. The homogenous, heterogenous and microbial-treated substrates via fermentation are used to rear BSFL and are compared in this review for the simultaneous production of larval protein and biodiesel. Moreover, the best moisture content and the aeration rate of larval feeding substrates are also reported in this review to enhance the growth of BSFL. As the downstream process after harvesting starts with larval inactivation, various related methods have also been reviewed in relation to its impact on the quality/quantity of larval protein and lipids. Subsequently, the other downstream processes, namely, extraction and transesterification to biodiesel, are finally epitomized from the literature to provide a comprehensive review for the production of unconventional protein and lipid sources from BSFL feedstock. Incontrovertibly, the review accentuates the great potential use of BSFL biomass as a green source of protein and lipids for energy production in the form of biodiesel. The traditional protein and energy sources, preponderantly fishmeal, are unsustainable naturally, pressingly calling for immediate substitutions to cater for the rising demands. Accordingly, this review stresses the benefits of using BSFL biomass in detailing its production from upstream all the way to downstream processes which are green and economical at the same time.
Publisher: Frontiers Media SA
Date: 25-02-2021
DOI: 10.3389/FENRG.2021.627093
Abstract: Anaerobic digestion (AD) from organic waste has gained worldwide attention because it offers significant environmental and economic benefits. It can reduce the local waste through recycling which will conserve resources, reduce greenhouse gas emissions, and build economic resilience in the face of an uncertain future for energy production and waste disposal. The productive use of local waste through recycling conserves resources by reducing landfill space, the whole of life impacts of landfilling, and post-closure maintenance of landfills. Turning waste into a renewable energy source will assist the decarbonisation of the economy by reducing harmful emissions and pollutants. Therefore, this mini-review aims to summarise key factors and present valuable evidence for an efficient AD process. It also presents the pros and cons of different AD process to convert organic waste along with the reactor technologies. Besides, this paper highlights the challenges and the future perspective of the AD process. However, it is highlighted that for an effective and efficient AD process, appropriate temperature, pH, a strong inoculum to substrate ratio, good mixing and small particle sizes are important factors. The selection of suitable AD process and reactor is important because not all types of processes and reactors are not effective for processing organic waste. This study is of great importance for ongoing work on renewable energy generation from waste and provides important knowledge of innovative waste processing. Finally, it is recommended that the government should increase their support towards the AD technology and consider the unutilized significant potential of gaseous biofuel production.
Publisher: Elsevier BV
Date: 09-2021
Publisher: Elsevier BV
Date: 07-2022
Publisher: Elsevier BV
Date: 2022
DOI: 10.1016/J.BIORTECH.2021.126399
Abstract: Biofuels have become an attractive energy source because of the growing energy demand and environmental issues faced by fossil fuel consumption. Algal biomass, particularly microalgae, has excellent potential as feedstock to be converted to bio-oil, biochar, and combustible syngas via thermochemical conversion processes. Third-generation biofuels from microalgal feedstock are the promising option, followed by the first-generation and second-generation biofuels. This paper provides a review of the applications of thermochemical conversion techniques for biofuel production from algal biomass, comprising pyrolysis, gasification, liquefaction, and combustion processes. The progress in the thermochemical conversion of algal biomass is summarized, emphasizing the application of pyrolysis for its benefits over other processes. The review also encompasses the challenges and perspectives associated with the valorization of microalgae to biofuels ascertaining the potential opportunities and possibilities of extending the research into this area.
Publisher: MDPI AG
Date: 19-02-2020
DOI: 10.3390/SU12041558
Abstract: The aim of this study was to spur the lipid accumulation by larvae of Hermetia illucens or black soldier fly (BSFL) via feeding with yeast fermented medium. The Saccharomyces cerevisiae, a single cell yeast, was introduced at different concentrations (0.02, 0.1, 0.5, 1.0, 2.5 wt %) to execute an in-situ fermentation on coconut endosperm waste. The rearing of BSFL was started simultaneously and the rearing was stopped once the BSFL reached the fifth instar. With the increasing of yeast concentration, the rearing duration of BSFL was shortened from 15.5 to 13.5 days. Moreover, it was found that at 0.5 to 1.0 wt % yeast concentration, the lipid yield and lipid productivity of BSFL were statistically enhanced to their highest peaks, namely, at 49.4% and 0.53 g/day, respectively. With regard to biodiesel composition, BSFL-derived biodiesel contained mainly C12:0, C14:0, C16:0 and C18:1. The higher amount of saturated fatty acids could strengthen the oxidative stability biodiesel produced as compared with non-edible oils or microalgal lipid. At last, the addition of yeast was also found to improve the waste reduction index of coconut endosperm waste (CEW) from 0.31 to 0.40 g/day, heralding the capability of BSFL to valorize organic waste via bioconversion into its biomass to serve as a feedstock for biodiesel production.
Publisher: MDPI AG
Date: 14-03-2020
DOI: 10.3390/PR8030337
Abstract: Recently, worldwide researchers have been focusing on exploiting of black soldier fly larval (BSFL) biomass to serve as the feed mediums for farmed animals, including aquaculture farming, in order to assuage the rising demands for protein sources. In this study, yeast was introduced into coconut endosperm waste (CEW) whilst serving as the feeding medium to rear BSFL in simultaneously performed in situ fermentation. It was found that at a 2.5 wt% yeast concentration, the total biomass gained, growth rate and rearing time were improved to 1.145 g, 0.085 g/day and 13.5 days, respectively. In terms of solid waste reduction, the inoculation of yeast over 0.5 wt% in CEW was able to achieve more than 50% overall degradation, with the waste reduction indexes (WRIs) ranging from 0.038 to 0.040 g/day. Disregarding the concentration of yeast introduced, the protein productivity from 20 BSFL was enhanced from only 0.018 g/day (the control) to 0.025 g/day with the presence of yeast at arbitrary concentrations. On the other hand, the larval protein yield was fortified from the control (28%) to a highest value of 35% with the presence of a mere 0.02 wt% yeast concentration. To summarize, the inclusion of a minimal amount of yeast into CEW for in situ fermentation ultimately enhanced the growth of BSFL, as well as its protein yield and productivity.
Publisher: Elsevier BV
Date: 02-2023
Publisher: Informa UK Limited
Date: 2020
Publisher: Elsevier BV
Date: 02-2020
DOI: 10.1016/J.BIORTECH.2019.122476
Abstract: Microalgal and lignocellulosic biomass is the most sumptuous renewable bioresource raw material existing on earth. Recently, the bioconversion of biomass into biofuels have received significant attention replacing fossil fuels. Pretreatment of biomass is a critical process in the conversion due to the nature and structure of the biomass cell wall that is complex. Although green technologies for biofuel production are advancing, the productivity and yield from these techniques are low. Over the past years, various pretreatment techniques have been developed and successfully employed to improve the technology. This paper presents an in-depth review of the recent advancement of pretreatment methods focusing on microalgal and lignocellulosic biomass. The technological approaches involving physical, chemical, biological and other latest pretreatment methods are reviewed.
Publisher: Elsevier BV
Date: 02-2022
Publisher: Elsevier BV
Date: 05-2022
DOI: 10.1016/J.ENVRES.2022.112685
Abstract: Graphene-based nanomaterials with remarkable properties, such as good biocompatibility, strong mechanical strength, and outstanding electrical conductivity, have dramatically shown excellent potential in various applications. Increasing surface area and porosity percentage, improvement of adsorption capacities, reduction of adsorption energy barrier, and also prevention of agglomeration of graphene layers are the main advantages of functionalized graphene nanocomposites. On the other hand, Cerium nanostructures with remarkable properties have received a great deal of attention in a wide range of fields however, in some cases low conductivity limits their application in different applications. Therefore, the combination of cerium structures and graphene networks has been widely invesitaged to improve properties of the composite. In order to have a comprehensive information of these nanonetworks, this research reviews the recent developments in cerium functionalized graphene derivatives (graphene oxide (GO), reduced graphene oxide (RGO), and graphene quantum dot (GQD) and their industrial applications. The applications of functionalized graphene derivatives have also been successfully summarized. This systematic review study of graphene networks decorated with different structure of Cerium have potential to pave the way for scientific research not only in field of material science but also in fluorescent sensing, electrochemical sensing, supercapacitors, and catalyst as a new candidate.
Publisher: Elsevier BV
Date: 09-2020
Publisher: Elsevier BV
Date: 12-2019
Publisher: Elsevier BV
Date: 09-2021
Publisher: Springer Science and Business Media LLC
Date: 20-04-2022
Publisher: MDPI AG
Date: 20-07-2023
DOI: 10.3390/MEMBRANES13070679
Abstract: Fruit juice is an essential food product that has received significant acceptance among consumers. Harmonized concentration, preservation of nutritional constituents, and heat-responsive sensorial of fruit juices are demanding topics in food processing. Membrane separation is a promising technology to concentrate juice at minimal pressure and temperatures with excellent potential application in food industries from an economical, stable, and standard operation view. Microfiltration (MF) and ultrafiltration (UF) have also interested fruit industries owing to the increasing demand for reduced pressure-driven membranes. UF and MF membranes are widely applied in concentrating, clarifying, and purifying various edible products. However, the rising challenge in membrane technology is the fouling propensity which undermines the membrane’s performance and lifespan. This review succinctly provides a clear and innovative view of the various controlling factors that could undermine the membrane performance during fruit juice clarification and concentration regarding its selectivity and permeance. In this article, various strategies for mitigating fouling anomalies during fruit juice processing using membranes, along with research opportunities, have been discussed. This concise review is anticipated to inspire a new research platform for developing an integrated approach for the next-generation membrane processes for efficient fruit juice clarification.
Publisher: Springer Science and Business Media LLC
Date: 11-02-2021
Publisher: Elsevier BV
Date: 04-2021
Publisher: Elsevier BV
Date: 03-2022
DOI: 10.1016/J.CHEMOSPHERE.2021.133006
Abstract: The paper evaluates the routes towards the evaluation of membranes using ZIF-62 metal organic framework (MOF) nano-hybrid dots for environmental remediation. Optimization of interaction of operating parameters over the rooted membrane is challenging issue. Subsequently, the interaction of operating parameters including temperature, pressure and CO
Publisher: Elsevier BV
Date: 09-2020
Publisher: MDPI AG
Date: 05-02-2021
DOI: 10.3390/PR9020299
Abstract: Coconut endosperm waste (CEW) was treated by Rhizopus oligosporus via in situ and ex situ fermentations together with bioconversion into valuable black soldier fly larval biomass. The ex situ fermentation could overall enrich the nutritional compositions of CEW by hydrolyzing its complex organic polymers and exuding assimilable nutrients to enhance the black soldier fly larvae (BSFL) growth. Nevertheless, the larval gut bacteria were competing with Rhizopus oligosporus in in situ fermentation, derailing the hydrolysis processes and larval growth. Accordingly, the highest growth rates achieved were around 0.095 g/day, as opposed to only 0.065 g/day whilst using 0.5 wt% of Rhizopus oligosporus to perform ex situ and in situ fermentations, respectively. These were also underpinned by the greater amount of total CEW consumed when employing ex situ fermentation, with comparable metabolic costs to feeding on in situ-fermented CEW. The mature BSFL were subsequently harvested and the amounts of protein and lipid produced were assessed in terms of their feasibility for biodiesel production. While the statistical analyses showed that the larval protein yields derived from both fermentation modes were insignificant, the BSFL could attain higher lipid and protein productivities upon feeding with ex situ- rather than in situ-fermented CEW mediums. Better yet, the larval biodiesel quality measured in terms of the fatty acid methyl ester composition were not varied significantly by Rhizopus oligosporus through the fermentation process. Thereby, the presence of 1.0 wt% Rhizopus oligosporus was considered optimum to perform ex situ fermentation, giving rise to the acceptable growth of BSFL loaded with the highest lipid yield and productivity for producing biodiesel and protein simultaneously.
Publisher: Informa UK Limited
Date: 22-12-2020
Publisher: Hindawi Limited
Date: 23-07-2020
DOI: 10.1002/ER.5699
Publisher: Hindawi Limited
Date: 15-06-2020
DOI: 10.1002/ER.5557
Publisher: Informa UK Limited
Date: 2019
Publisher: Springer Science and Business Media LLC
Date: 17-02-2021
Publisher: Akademiai Kiado Zrt.
Date: 13-06-2022
Abstract: Gamma-aminobutyric acid (GABA), a four-carbon non-protein amino acid, is widely known to have multiple physiological functions. The present study aimed to investigate the cultivation parameters for GABA production by a lactic acid bacteria (LAB) strain isolated from a tuna gut s le. Among 60 tuna gut LAB, only 7 Limosilactobacillus fermentum isolates, i.e. NG01, NG12, NG13, NG14, NG16, NG23, and NG27, were capable of GABA fermentation, with NG16 being the most potent GABA producer. The GABA production by isolate NG16 was therefore thoroughly characterised. The optimal batch culture conditions for GABA production were an initial cell density of 5×10 6 CFU mL −1 , a monosodium glutamate concentration of 2%, an initial pH of 7, a fermentation temperature of 35 °C, and an incubation time of 96 h. Under this cultivation conditions, NG16 produced a maximum GABA yield of 25.52 ± 0.41 mM.
No related grants have been discovered for Show Pau Loke.