ORCID Profile
0000-0002-0478-7045
Current Organisations
Nepal Agricultural Research Council
,
Himalayan College of Agricultural Sciences and Technology
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Publisher: Science Alert
Date: 15-06-2016
Publisher: Elsevier BV
Date: 02-2001
Publisher: Springer Science and Business Media LLC
Date: 06-10-2014
Publisher: O.I.E (World Organisation for Animal Health)
Date: 12-2016
Abstract: Cattle play an important role in the epidemiology of bluetongue (BT) by acting as reservoir hosts. However, the status of BT virus (BTV) in dairy cattle in Nepal is unknown. The objective of this study was to estimate the prevalence of BTV antibodies in dairy cattle in two eco-zones of Nepal, and to identify the factors associated with virus exposure. The authors conducted a cross-sectional serosurvey from March 2012 through February 2013 by s ling 131 dairy cattle from seven clusters (villages) in the Chitwan district in the Terai region (southern lowlands) and the Lamjung district in the Hills region (the middle part of Nepal). Of the 131 serum s les tested, 29.3% (95% confidence interval [CI]: 21.5-37.2) were positive for BTV antibodies. Herd-level seroprevalence was 45.7% (95% CI: 30.9-61.0). Bivariate analysis indicated a positive association between seroconversion to BTV and age, and an association with breed of cattle after controlling for clustering of animals within herds. Based on this model, cattle were more likely to become seropositive as they aged. Crossbred cattle were more likely to be seropositive than those of exotic breeds (odds ratio [OR] = 4.6 95% CI: 1.5-14.1). The results indicate widespread exposure of dairy cattle to BTV in Nepal. The authors suggest that dairy cattle should be included in the surveillance plan for BTV infection in Nepal and that it is important to educate farmers about the possible impacts of this disease.
Publisher: Nepal Journals Online (JOL)
Date: 27-03-2018
DOI: 10.3126/IJASBT.V6I1.19103
Abstract: Mycoplasmas are amongst the avian pathogens that causes chronic respiratory and joint diseases incurring a huge economic loss to poultry industry of Nepal. Among different species of Mycoplasmas, we investigate Mycoplasma gallisepticum-synoviae from the serum s les of the poultry using Enzyme Linked Immuno Sorbent Assay (ELISA) and dot-ELISA test of ImmunoComb. These tests rely on the antibodies present in the serum s les which binds to the pre-coated antigen in the ELISA/developing plates. A total of 92 sera s les were collected, of which 62 were from broiler and 30 were from layers. Of the 92 s les taken from different farms of Bhaktapur in early 2017, thirty (32.6%) were found positive on standard or routine ELISA and fifty-four (58.7%) on dot-ELISA. It further indicates high rate of infections in broiler (42 on dot-ELISA and 24 on standard ELISA) than in layers (12 in dot-ELISA and 6 on standard ELISA) to M. gallisepticum and M. synoviae. The present result indicates higher efficacy of dot-ELISA over standard one as the latter could not efficiently detect the infections as compared to the dot-ELISA. Furthermore, this finding poses an alarming threat to the commercial poultry industries of Nepal where there is lack of effective biosecurity practices and preventive vaccination policy. Int. J. Appl. Sci. Biotechnol. Vol 6(1): 23-26
Publisher: Elsevier BV
Date: 15-05-2002
Publisher: Elsevier BV
Date: 08-2010
Location: Japan
Location: Nepal
No related grants have been discovered for Doj Raj Khanal.