ORCID Profile
0000-0002-1809-5955
Current Organisation
University of California, San Diego
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Publisher: Wiley
Date: 14-01-2022
Abstract: The transmission of SARS‐CoV‐2 coronavirus has led to the COVID‐19 pandemic. Nucleic acid testing while specific has limitations for mass surveillance. One alternative is the main protease (M pro ) due to its functional importance in mediating the viral life cycle. Here, we describe a combination of modular substrate and gold colloids to detect M pro via visual readout. The strategy involves zwitterionic peptide that carries opposite charges at the C‐/N‐terminus to exploit the specific recognition by M pro . Autolytic cleavage releases a positively charged moiety that assembles the nanoparticles with rapid color changes ( t min). We determine a limit of detection for M pro in breath condensate matrices nM. We further assayed ten COVID‐negative subjects and found no false‐positive result. In the light of simplicity, our test for viral protease is not limited to an equipped laboratory, but also is amenable to integrating as portable point‐of‐care devices including those on face‐coverings.
Publisher: American Chemical Society (ACS)
Date: 18-01-2022
Publisher: American Chemical Society (ACS)
Date: 14-09-2021
Publisher: American Chemical Society (ACS)
Date: 24-03-2021
Publisher: American Chemical Society (ACS)
Date: 26-07-2021
Publisher: Wiley
Date: 14-01-2022
Abstract: The transmission of SARS‐CoV‐2 coronavirus has led to the COVID‐19 pandemic. Nucleic acid testing while specific has limitations for mass surveillance. One alternative is the main protease (M pro ) due to its functional importance in mediating the viral life cycle. Here, we describe a combination of modular substrate and gold colloids to detect M pro via visual readout. The strategy involves zwitterionic peptide that carries opposite charges at the C‐/N‐terminus to exploit the specific recognition by M pro . Autolytic cleavage releases a positively charged moiety that assembles the nanoparticles with rapid color changes ( t min). We determine a limit of detection for M pro in breath condensate matrices nM. We further assayed ten COVID‐negative subjects and found no false‐positive result. In the light of simplicity, our test for viral protease is not limited to an equipped laboratory, but also is amenable to integrating as portable point‐of‐care devices including those on face‐coverings.
Location: United States of America
No related grants have been discovered for Colman Moore.