Publication
Plasma amino acid concentrations during experimental hyperinsulinemia in 2 laminitis models
Publisher:
Wiley
Date:
11-03-2021
DOI:
10.1111/JVIM.16095
Abstract: Endocrinopathic laminitis develops in association with insulin dysregulation, but the role of insulin in the pathogenesis remains unclear. Hyperinsulinemia can cause hypoaminoacidemia, which is associated with integumentary lesions in other species and therefore warrants investigation as a potential mechanism in laminitis. Evaluate plasma amino acid concentrations in the euglycemic‐hyperinsulinemic cl (EHC) and prolonged glucose infusion (PGI) laminitis models. Sixteen Standardbred horses. Prospective experimental study. Plasma amino acid concentrations were measured in s les collected every 6 hours from horses that underwent a 48‐hour EHC (n = 8) or 66‐hour PGI (n = 8) after a 24‐ or 6‐hour baseline period in EHC and PGI groups, respectively. Fifteen of the 20 measured amino acid concentrations decreased over time in both EHC and PGI horses ( P 0.001). The median percentage change from baseline for these amino acids was: histidine (EHC: 41.5% PGI: 43.9%), glutamine (EHC: 51.8% PGI: 35.3%), arginine (EHC: 51.4% PGI: 41%), glutamic acid (EHC: 52.4% PGI: 31.7%), threonine (EHC: 62.8% PGI: 25.2%), alanine (EHC: 48.9% PGI: 19.5%), proline (EHC: 56.2% PGI: 30.3%), cystine (EHC: 34.9% PGI: 31.2%), lysine (EHC: 46.4% PGI: 27.8%), tyrosine (EHC: 27.5% PGI: 16.9%), methionine (EHC: 69.3% PGI: 50.8%), valine (EHC: 50.8% PGI: 34.4%), isoleucine (EHC: 60.8% PGI: 38.7%), leucine (EHC: 48.2% PGI: 36.6%), and phenylalanine (EHC: 16.6% PGI: 12.1%). Hypoaminoacidemia develops in EHC and PGI laminitis models. The role of hypoaminoacidemia in the development of hyperinsulinemia‐associated laminitis warrants further investigation.