ORCID Profile
0000-0002-6264-6961
Current Organisation
Harvard T.H. Chan School of Public Health
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Publisher: American Society for Microbiology
Date: 07-2019
DOI: 10.1128/AEM.00137-19
Abstract: Historical antimicrobial use in NZ agriculture has driven the evolution of ST108, a VRE lineage carrying a range of clinically relevant antimicrobial resistances. The persistence of this lineage in NZ for over a decade indicates that coselection may be an important stabilizing mechanism for its persistence.
Publisher: Oxford University Press (OUP)
Date: 20-12-2021
DOI: 10.1093/NAR/GKAB1210
Abstract: During infection, phages manipulate bacteria to redirect metabolism towards viral proliferation. To counteract phages, some bacteria employ CRISPR-Cas systems that provide adaptive immunity. While CRISPR-Cas mechanisms have been studied extensively, their effects on both the phage and the host during phage infection remains poorly understood. Here, we analysed the infection of Serratia by a siphovirus (JS26) and the transcriptomic response with, or without type I-E or I-F CRISPR-Cas immunity. In non-immune Serratia, phage infection altered bacterial metabolism by upregulating anaerobic respiration and amino acid biosynthesis genes, while flagella production was suppressed. Furthermore, phage proliferation required a late-expressed viral Cas4 homologue, which did not influence CRISPR adaptation. While type I-E and I-F immunity provided robust defence against phage infection, phage development still impacted the bacterial host. Moreover, DNA repair and SOS response pathways were upregulated during type I immunity. We also discovered that the type I-F system is controlled by a positive autoregulatory feedback loop that is activated upon phage targeting during type I-F immunity, leading to a controlled anti-phage response. Overall, our results provide new insight into phage-host dynamics and the impact of CRISPR immunity within the infected cell.
Publisher: American Society for Microbiology
Date: 16-12-2021
DOI: 10.1128/MRA.01050-21
Abstract: Here, we report the genome sequences of three bacterial isolates, Kinneretia sp. strain XES5, Shinella sp. strain XGS7, and Vogesella sp. strain XCS3, which were cultured from skin of adult female laboratory-bred Xenopus laevis .
Publisher: Cold Spring Harbor Laboratory
Date: 12-10-2017
DOI: 10.1101/202077
Abstract: Environmental DNA sequencing has rapidly become a widely-used technique for investigating a range of questions, particularly related to health and environmental monitoring. There has also been a proliferation of bioinformatic tools for analysing metagenomic and licon datasets, which makes selecting adequate tools a significant challenge. A number of benchmark studies have been undertaken however, these can present conflicting results. We have applied a robust Z-score ranking procedure and a network meta-analysis method to identify software tools that are generally accurate for mapping DNA sequences to taxonomic hierarchies. Based upon these results we have identified some tools and computational strategies that produce robust predictions.
Location: No location found
Location: United States of America
Location: United States of America
No related grants have been discovered for Xochitl Morgan.