ORCID Profile
0000-0002-9480-5330
Current Organisation
University of Adelaide
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Publisher: Springer Science and Business Media LLC
Date: 2003
Publisher: Elsevier BV
Date: 2023
DOI: 10.1016/J.EXPPARA.2022.108409
Abstract: Many recent studies have been conducted to find new DNA vaccines based on Toxoplasma gondii antigens. DNA vaccines encoding complex of different antigens showed better immune responses compared to single antigen vaccine. In this study, we constructed a DNA vaccine encoding SAG1, SAG3, MIC4, GRA5, GRA7, AMA1 and BAG1 against T. gondii, and evaluated the immune response it induced in BALB/c mice. For this purposes, thirty BALB/c mice were randomly ided into three groups containing tenmice each. There were two negative control groups (PBSand pVAX1 vector) and one vaccination group (pVAX1-MAF, Multantigenic Fragment). On days 0, 14 and 28, the mice were immunized intramuscularly, and 5 weeks later they were challenged with T. gondii RH strain. The immune responses were evaluated using lymphocyte proliferation assay, T-cell subsets detection, and measurement of antibody and cytokine levels. The results showed that mice immunized with pVAX1-MAF developed high levels of IL-2, IL-12, IgG and IFN- γ as well as CD3
Publisher: Science Alert
Date: 15-06-2005
Publisher: Elsevier BV
Date: 08-2010
DOI: 10.1016/J.VETPAR.2010.04.003
Abstract: Neospora caninum is an intracellular protozoan that infects domestic and wild canids, as well as many warm blooded animals as shown by the isolation of viable parasites and the detection of parasite DNA in naturally infected hosts. N. caninum is described as a cause of neuromuscular disease and death in dogs. The dog is also known as a definitive host, shedding oocysts involved in the transmission of the infection to intermediate hosts. This study was conducted to develop an indirect ELISA test using an affinity purified 38 kDa N. caninum surface antigen (P38) for the sensitive and specific diagnosis of this infection in dog populations. To define a suitable cut-off, serum s les from 233 dogs were analyzed using an N. caninum-specific indirect fluorescent antibody test. All of these serum s les were subjected to the newly designed P38-ELISA. The Two-graph Receiver Operating Characteristics (TG-ROC) of the serum ELISA was determined to examine the performance of the test. TG-ROC analysis showed an area under curve (AUC) of 0.996 that indicates the test results being highly accurate. Optimal sensitivity and specificity (100% and 97.9%, respectively) were determined for SI(n) cut-off point of 0.23. To examine possible cross-reactions with other parasites affecting dogs, sera of dogs positive for antibodies against Toxoplasma gondii (n=17) and Leishmania infantum (n=11) infections were tested. These revealed negative results when tested in the new ELISA.
Publisher: Van Yuzuncu Yil University
Date: 30-04-2021
DOI: 10.52976/VANSAGLIK.808712
Abstract: Küçükbaş ruminantların morbillivirusu (SRMV), daha önceki adıyla peste-des-petits ruminants virus (PPRV) enfeksiyonu, küçükbaş ruminantların ekonomik olarak en önemli hastalıklarından biridir. Bu çalışmada, İran'daki küçükbaş ruminantlarda PPRV enfeksiyonunun seroprevalansının belirlenmesi amaçlanmıştır. Bu bağlamda, İran’ın 9 farklı eyaletindeki (Ardabil, Azarbayjan-e Gharbi, Azarbayjan-e Sharqi, Hormozgan, Esfahan, Fars, Kordestan, Gilan, Shahrekord) işletmelerden elde edilen ve İran/Şehrekord Üniversitesi tanı laboratuvarı stoklarında bulunan kan serumu örnekleri kullanılmıştır. Söz konusu işletmelerde PPRV enfeksiyonuna karşı aşılama yapılmadığı öğrenilmiştir. PPRV, N proteinine karşı gelişen spesifik antikorların tespiti amacıyla, 9 farklı eyaletten elde edilen ve İran/Shahrekord Üniversitesi tanı laboratuvarına getirilen 444 koyun ve 58 keçiden alınan toplam 502 kan serumu örneği C-ELISA kiti (ID Screen® PPRV Competition) ile kontrol edilmiştir. PPRV seropozitiflik oranı % 53,38 (268/502) olarak tespit edilmiştir. Örnek alınan eyaletlerden Gilan hariç hepsi PPRV yönünden seropozitif olarak tespit edilmiş ve eyaletlere göre seropozitiflik dağılımının %12.5 - 96.29 oranları arasında değişim gösterdiği belirlenmiştir. Hayvan türlerine göre seropozitiflik değerlerine bakıldığında ise, örneklenen koyunların %53.6’sı (238/444) ve keçilerin %51.7’si (30/58) PPRV spesifik antikorları yönünden pozitif olarak bulunmuştur. PPRV enfeksiyonunun İran’da endemik olduğu bir kez daha ortaya konmuş Türkiye’nin sınır komşusu olan İran ve hayvan hareketlerinin sınırlandırılmasının zorluğuna dikkat çekilerek, enfeksiyondan korunmada en etkili yolun duyarlı populasyonun aşılanması üzerinde durulması gerekliliği sonucuna varılmıştır.
Publisher: Springer Science and Business Media LLC
Date: 15-10-2009
Publisher: Briefland
Date: 18-10-2020
DOI: 10.5812/JJCMB.108799
Abstract: Background: Staphylococcus aureus (S. aureus) is a major cause of nosocomial infections in humans and animals. Because of the widespread resistance to antibiotics, microbiologists are trying to find other therapeutic interventions such as phage therapy for bacterial infections. Objectives: The present study aimed to isolate staphylophages with lytic effects on methicillin-resistant S. aureus (MRSA) clinical isolates as a potential alternative agent to antibiotic therapy. Methods: This experimental, descriptive study is performed in the Microbiology Laboratory of Shahrekord University (Iran) from September 2018 to March 2019. Two cocktails of staphylophages were isolated from Isfahan (Iran) urban sewage s les. The double-layer agar method was used to detect lytic phages. Morphology characteristic by transmission electron microscopy (TEM) images was used to identify staphylophages. One hundred and thirty three S. aureus were isolated from clinical s les of two teaching hospitals in Isfahan and Shiraz, Iran. Methicillin resistance and the presence of the mecA gene were determined by the disk diffusion method and polymerase chain reaction (PCR) assay, respectively. The phage susceptibility of mecA positive isolates was determined by plaque assay. Results: Two staphylophage cocktails were prepared, which had lytic effects on forty-four MRSA isolates. Cocktails 1 and 2 lysed 19 (14.2%) and 25 (18.7%) isolates, respectively. Of 133 S. aureus isolates, 88.7% carried the mecA gene. Conclusions: Different bacteriophages in two phage cocktails had relatively good lytic effects on S. aureus clinical isolates. Therefore, phage cocktails may be an appropriate alternative to antibiotics against S. aureus.
Publisher: Briefland
Date: 03-2014
DOI: 10.5812/JJM.9394
Publisher: Mary Ann Liebert Inc
Date: 09-2012
Publisher: Medknow
Date: 2016
Publisher: Springer Science and Business Media LLC
Date: 07-04-2009
Publisher: Briefland
Date: 04-09-2016
DOI: 10.17795/JJNPP-32852
Publisher: Elsevier BV
Date: 12-2013
Publisher: Science Alert
Date: 2006
Publisher: Springer Science and Business Media LLC
Date: 16-05-2009
Publisher: Briefland
Date: 06-08-2016
DOI: 10.17795/IJI-38768
Publisher: Science Alert
Date: 15-04-2005
Publisher: Briefland
Date: 16-08-2016
DOI: 10.17795/ZJRMS-2482
Publisher: Science Alert
Date: 15-07-2005
Publisher: Informa UK Limited
Date: 02-11-2014
DOI: 10.1080/03079457.2014.966648
Abstract: The virus genus Circovirus belongs to the family Circoviridae and contains virus species with circular single-stranded DNA genomes. The viruses are known to infect vertebrate species, including pigs, dogs, pigeons and ducks. In this study a nested polymerase chain reaction (PCR) was applied to investigate prevalence of circoviruses in pigeons in the Chaharmahal va Bakhtiari province, Iran. A total of 50 faecal s les were subjected to nucleic acid extraction, PCR lification and DNA sequencing. Nested PCR primers were designed to lify a 508 base pair segment of the pigeon circovirus (PiCV) capsid gene. Of the 50 faecal s les examined, 12 (24%) produced the expected DNA licons with identical DNA sequences. Phylogenetic analysis has grouped the viruses with those classified as group A circoviruses. The viruses were closely related to PiCVs found in Poland, Northern Ireland, the USA, Nigeria and Hungary. To the authors' knowledge, this is the first report of molecular detection and genomic characterization of PiCV from Iran.
Publisher: Science Alert
Date: 15-09-2005
Publisher: Briefland
Date: 04-09-2017
DOI: 10.5812/JJNPP.32852
Publisher: Elsevier BV
Date: 12-2012
Publisher: Briefland
Date: 19-04-2023
DOI: 10.5812/JJCMB-135397
Abstract: : Border disease virus (BDV) is a serious pathogen of sheep and goats worldwide. The virus causes considerable economic losses in animal husbandry. Therefore, developing effective and genetic-based therapies to control viral infections, such as BDV, has been remarkable over recent years. A common way to evaluate such therapeutic strategies is by cloning the desired fragment into appropriate vectors to develop cell lines expressing it. Due to the required duties of the NS3 gene for BDV proliferation, this gene's HELICc and nucleotide binding site domain was synthesized and cloned in a lentiviral vector upstream of the GFP gene. The cloning accuracy was verified by digestion with restriction enzymes and sequencing. Thus, the BDV-NS3 HELICc and nucleotide binding site carrying plasmid was prepared successfully and will be applied in a second or third lentiviral packaging system for stable expression of the desired gene.
Publisher: Briefland
Date: 25-05-2015
Publisher: Science Alert
Date: 04-2006
Publisher: Science Alert
Date: 02-2006
Publisher: Briefland
Date: 30-08-2016
DOI: 10.17795/IJI-40296
Publisher: Briefland
Date: 08-04-2014
DOI: 10.17795/JJNPP-15402
Publisher: Science Alert
Date: 15-06-2007
Publisher: Briefland
Date: 08-11-2015
DOI: 10.5812/JJM.26590
Publisher: Springer Science and Business Media LLC
Date: 18-06-2008
Publisher: Science Alert
Date: 05-2006
Location: Iran (Islamic Republic of)
No related grants have been discovered for Mohammadreza Mahzounieh.