ORCID Profile
0000-0001-6080-9547
Current Organisations
University of Tsukuba
,
James Cook University
,
Universitas Padjadjaran
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Publisher: Frontiers Media SA
Date: 14-10-2019
Publisher: CSIRO Publishing
Date: 2010
DOI: 10.1071/CP10084
Abstract: The expression of morphological, phenological and agronomic traits was recorded in parents and progeny of hybrids between domesticated and wild accessions of the tropical tuberous legume Vigna vexillata (L.) A. Rich. Generally similar patterns were observed in hybrid populations between the putative domesticate var. macrosperma and wild Australian and African accessions, and between a cultivated Bali variety and a wild Australian accession, although observations in the latter populations were severely constrained by genetic incompatibilities between the parents. Several traits of domestication, including broad leaflet size, ovate leaflet shape, non-pigmented stems, non-dehiscent pods, uniform seed testa colour and green seed testa were recessive to the wild type traits and under simple genetic control, with genetic linkages evident between several wild type traits. Most morphological and phenological traits were under quantitative genetic control with high to very high broad sense heritability and moderate to very high narrow sense heritability, indicating good prospects for advance through breeding and selection. An exception was time to flowering, where there were discontinuities in frequency distributions of F2 and backcross populations, with some apparent dominance for earliness over lateness. Another exception was the number of seeds per pod, which in the var. macrosperma × wild populations, appeared to be under qualitative di-allelic control, with homozygous plants having nearly twice the number of seeds per pod as heterozygous plants. In some populations, there was apparent hybrid vigour for vegetative growth and tuber size attributes. It was concluded that the wild African and Australian accessions could be used along with var. macrosperma for breeding improved varieties of V. vexillata for forage, cover crop and vegetable uses. However, for improved pod and seed attributes, particularly the yield, size and appearance of seeds, emphasis needs to be placed on sourcing greater genetic ersity within var. macrosperma and within the cultivated Bali variety, which, because of their genetic incompatibility, will need to be the focus of separate breeding programs.
Publisher: MDPI AG
Date: 22-11-2022
DOI: 10.3390/HORTICULTURAE8121098
Abstract: A longer shelf-life for tomatoes without pleiotropic effects is one of the main goals of breeding programs in tropical countries. Therefore, this study aimed to evaluate the effect of the Sletr1-2 mutant allele on flowering, fruit phenotype, shelf life, and yield-related traits in four F1 hybrids from four tropical tomato genetic backgrounds. The study consisted of four tropical strains, namely ‘Intan’, ‘Mirah’, ‘Ratna’, and ‘Mutiara’, as females crossed with wild type ‘Micro-Tom’ (WT-MT) and mutant Sletr1-2 as males. Each was given three treatments and analyzed separately using a randomized block design with four replications of five s les each. The next test used was the Tukey Alpha 0.05 test. The genetic background of tropical tomatoes affects the phenotype and shelf-life. F1 mutants ‘Intan’ and ‘Ratna’ showed significant results, with a longer shelf-life than F1 WT (10.2 and 14.6 days, respectively). In addition, there were no side effects of the Sletr1-2 mutant allele in the heterozygous form on flowering, fruit phenotype, and yield. In conclusion, the Sletr1-2 allele has the potential to be used in tomato breeding programs in tropical countries.
Publisher: MDPI AG
Date: 12-12-2022
Abstract: Chinese sprangletop (Leptochloa chinensis (L.) Nees) is recognized as the most disturbing weed in rice fields in Africa, Australia, and Asia due to causing a significant reduction in yields. The habit of most lowland rice farmers in Indonesia is using high doses of herbicides more than once without any rotation, leading to increased weed resistance potential. Therefore, this study aimed to confirm the resistance level of L. chinensis to acetyl-CoA carboxylase (ACCase)-inhibiting herbicides using the whole-plant pot test method. We identified other herbicides that can control the resistant biotype and performed DNA sequencing on a s le to determine mutations present in the biotype′s ACCase gene through polymerase chain reaction. The herbicide dose–response experiment showed that the L. chinensis from Bantimurung Subdistrict, Maros Regency, South Sulawesi, exhibited resistance to ACCase inhibitors (metamifop and cyhalofop-butyl), which is the first case of resistance to ACCase herbicides reported in Indonesia. An acetolactate synthase inhibitor (bispyribac-sodium) and a 1-deoxy-d-xylulose-5-phosphate synthase (DOXP) inhibitor (clomazone) were effective at controlling the resistant biotypes of L. chinensis, so could considered for use in rotation or as an ingredient in mixed herbicides. Single-nucleotide substitution of guanine for thiamine at position 6081 (TGG susceptible, TGT Maros) that causes a Trp2027Cys mutation in the target gene contributes to the resistance of the Maros biotype to ACCase inhibitors.
Publisher: CSIRO Publishing
Date: 2010
DOI: 10.1071/CP10029
Abstract: Vigna vexillata is an underutilised perennial tuberous legume with several multipurpose uses. In a comparative study to identify sources of potentially useful genetic variation, six cultivated accessions from two regions in Bali, a var. macrosperma accession and 12 wild accessions from Africa, Australia and Indonesia were evaluated for selected morphological, agronomic and phenological traits. Measurements were made of qualitative and quantitative traits on plants grown over the spring–autumn period in large pots on benches outdoors at Townsville, Australia (19°19.7′S, 146°45.7′E). Variation among the genotypes was assessed between the three main groups of accessions, between provenances within groups, and between accessions within provenances. Large variation was observed for most traits, with clear differentiation evident between the three main groups of accession. There was minimal variation within the Bali accessions for all except tuber attributes, while within the wild accessions there were some small differences due to provenance. The Bali accessions and var. macrosperma shared many attributes often associated with domestication, distinguishing them from the wild accessions. For ex le, the Bali accessions and var. macrosperma had broad-ovate leaflets, uniform-coloured seed testa, and non-dehiscent pods, and larger pods and seeds, seed yield per plant and seed harvest index than the wild accessions. Nonetheless, there were distinct differences between the Bali accessions and var. macrosperma. The Bali accessions had a larger seed size but fewer seeds per pod than var. macrosperma and were unique in having more than twice as many flowers per peduncle and a much longer duration of seed development than either var. macrosperma or the wild accessions. Whereas the Bali accessions were photoperiod-sensitive and as a consequence, late flowering and vegetatively vigorous, var. macrosperma was early flowering, more bush-like in habit and had higher seed yield and seed harvest index, all traits associated with domestication. Across all the accessions, there were significant correlations between many pairs of traits. While the present study identified sources of potentially useful variation, it was concluded that the lack of variation within the Bali accessions, combined with the paucity of both cultivated and var. macrosperma accessions in current germplasm collections, pose constraints to the future genetic improvement of V. vexillata.
Publisher: CSIRO Publishing
Date: 2010
DOI: 10.1071/CP10060
Abstract: Phenotypic similarities and differences between a cultivated variety of the tuberous legume Vigna vexillata from Bali, Indonesia, the putative domesticated variety macrosperma and wild types from Africa, Australia and Indonesia, were reported previously. The present study was undertaken to establish the genetic compatibility of these three accession classes. Seventeen accessions, comprising eight cultivated Bali accessions, one var. macrosperma accession and eight wild accessions from Africa and Austronesia, were grown in large pots in shade house facilities in Townsville, Australia. Not all hybrid combinations were attempted because for some accession combinations, suitable matching flowers were not available at the same time. The main aim was to attempt enough crosses between accessions from the respective classes to establish whether the classes were genetically compatible. Hybridisation was conducted by hand pollination in the morning, using newly-open flowers that had been emasculated before sunset on the day before. Pods and viable hybrid seed were obtained from the Bali × Bali, var. macrosperma × wild and wild African × wild Austronesian combinations. However, difficulty was encountered in obtaining viable and/or self-fertile hybrids between the Bali accessions and the other two classes. Depending on the particular combination of parental accessions, different genetic breakdown mechanisms were observed with the Bali × var. macrosperma and Bali × wild combinations. In some instances, flowers failed to set pods and/or the young pods abscised before maturity pods set but seed were shrivelled and/or non-viable viable seeds were set but the hybrid seedling plants were short-lived or, in a few instances (Jimbaran Bali × wild Austronesian), vigorous hybrid plants were obtained but were self-sterile. Mitotic chromosome counts showed there was no difference in chromosome number between the Bali accessions, the Austronesian accessions and those hybrids that were viable but infertile. All exhibited 2n = 22. Pollen viability analyses using Alexander’s stain indicated that the numbers of pollen grains per flower and the percentages of pollen grain that were viable were substantially lower in the hybrids than in both the cultivated Bali and wild parental accessions. Consistent with this observation, small numbers of viable seeds were obtained when viable pollen from the respective parents was backcrossed onto the self-sterile hybrids. The results suggested that the cultivated Bali accessions do not belong to the same primary gene pool as the other cultivated and wild V. vexillata accessions and that it would be difficult to use the Bali accessions and var. macrosperma concurrently to breed seed crop varieties. A taxonomic review of V. vexillata is also warranted.
No related grants have been discovered for Farida Damayanti.