ORCID Profile
0000-0002-5553-5147
Current Organisations
University of Graz
,
Research Center Pharmaceutical Engineering GmbH
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Publisher: American Chemical Society (ACS)
Date: 13-10-2014
DOI: 10.1021/MP500264E
Publisher: American Chemical Society (ACS)
Date: 05-06-2014
DOI: 10.1021/JP502330E
Publisher: American Chemical Society (ACS)
Date: 04-02-2013
DOI: 10.1021/CG301812M
Publisher: American Chemical Society (ACS)
Date: 08-12-2014
DOI: 10.1021/CG501391J
Publisher: Beilstein Institut
Date: 06-07-2015
DOI: 10.3762/BJNANO.6.151
Abstract: The small intestine is a complex system that carries out various functions. The main function of enterocytes is absorption of nutrients, whereas membranous cells (M cells) are responsible for delivering antigens/foreign substances to the mucosal lymphoid tissues. However, to get a fundamental understanding of how cellular structures contribute to physiological processes, precise knowledge about surface morphologies, cytoskeleton organizations and biomechanical properties is necessary. Atomic force microscopy (AFM) was used here as a powerful tool to study surface topographies of Caco-2 cells and M cells. Furthermore, cell elasticity (i.e., the mechanical response of a cell on a tip indentation), was elucidated by force curve measurements. Besides elasticity, adhesion was evaluated by recording the attraction and repulsion forces between the tip and the cell surface. Organization of F-actin networks were investigated via phalloidin labeling and visualization was performed with confocal laser scanning fluorescence microscopy (CLSM) and scanning electron microscopy (SEM). The results of these various experimental techniques revealed significant differences in the cytoskeleton/microvilli arrangements and F-actin organization. Caco-2 cells displayed densely packed F-actin bundles covering the entire cell surface, indicating the formation of a well-differentiated brush border. In contrast, in M cells actins were arranged as short and/or truncated thin villi, only available at the cell edge. The elasticity of M cells was 1.7-fold higher compared to Caco-2 cells and increased significantly from the cell periphery to the nuclear region. Since elasticity can be directly linked to cell adhesion, M cells showed higher adhesion forces than Caco-2 cells. The combination of distinct experimental techniques shows that morphological differences between Caco-2 cells and M cells correlate with mechanical cell properties and provide useful information to understand physiological processes/mechanisms in the small intestine.
Publisher: American Chemical Society (ACS)
Date: 24-02-2014
DOI: 10.1021/CG401859P
Publisher: American Chemical Society (ACS)
Date: 18-08-2015
Publisher: American Chemical Society (ACS)
Date: 15-01-2014
DOI: 10.1021/MP4005782
Abstract: Drying is a common pharmaceutical process, whose potential to alter the final drug properties-even at relatively low temperatures-is often neglected. The present study addresses the impact of drying at 20 and 50 °C on wet-extruded calcium stearate (CaSt) pellets. Drying at 20 °C caused the majority of ibuprofen to accumulate at the pellet surface due to a strong convective flow from the pellet's center to the surface. In contrast, pellets dried at 50 °C still contained ibuprofen in the pellet's interior due to the higher drying rate and the associated film breakage during drying. Moreover, the higher drying temperature caused CaSt to form a second lamellar phase and ibuprofen to convert (partly) into its amorphous state. Overall, the drying process affected the solid state and the spatial ibuprofen distribution within the pellet. Knowledge of these effects can aid in tailoring advanced multipellet formulations.
Publisher: American Chemical Society (ACS)
Date: 17-01-2014
DOI: 10.1021/MP4006479
No related grants have been discovered for Eva Roblegg.