ORCID Profile
0000-0002-7625-5259
Current Organisation
Microba Life Sciences
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Publisher: Wiley
Date: 2011
Publisher: Springer Science and Business Media LLC
Date: 04-2005
Publisher: Wiley
Date: 10-2009
DOI: 10.1577/M08-234.1
Publisher: Wiley
Date: 12-05-2005
Publisher: Wiley
Date: 11-04-2011
Publisher: Springer Science and Business Media LLC
Date: 02-2016
Publisher: Wiley
Date: 11-03-2012
DOI: 10.1111/J.1365-2761.2012.01345.X
Abstract: Overfished species of rockfish, Sebastes spp., from the Northeast Pacific experience high bycatch mortality because of 'barotrauma', a condition induced from the rapid change in pressure during capture. Field experiments show that it may be possible for rockfish to recover from barotrauma if quickly recompressed however, no work has followed the physiological recovery of rockfish after recompression or determined whether it is possible for rockfish to survive barotrauma in the long term. Barotrauma was induced in adult black rockfish, Sebastes melanops Girard, from a simulated depth of 35 m, followed by recompression. Blood and selected tissues (eye, heart ventricle, head kidney, liver, rete mirabile and gonad) were s led at days 3, 15 and 31 post-recompression to evaluate the tissue- and physiologic-level response during recovery. No mortality from barotrauma occurred during the experiments, and feeding resumed in 80% of both treatment and control fish. The primary injury in treatment fish was the presence of a ruptured swimbladder and/or a ruptured tunica externa (outer layer of swimbladder), which was slow to heal. Blood plasma was analysed for glucose, sodium, chloride, potassium, calcium, phosphorus, insulin-like growth factor-1 and cortisol. Plasma analyses indicated no strong effects because of barotrauma, suggesting overall handling stress outweighed any effect from barotrauma. Rockfish with ruptured swimbladders may face compromised competency in the wild however, it appears the majority of black rockfish decompressed from 35 m have a high potential for recovery if recompressed immediately after capture. This research suggests recompression could be a valuable bycatch mortality reduction tool for rockfish in recreational fisheries.
Publisher: Wiley
Date: 25-05-2012
DOI: 10.1111/J.1095-8649.2012.03322.X
Abstract: A Sebastes-specific complementary DNA (cDNA) microarray was developed to identify potential biomarkers involved in the capture stress and recovery of Sebastes species if they are assisted in returning to their original depth of capture following barotrauma. Black rockfish Sebastes melanops were exposed to simulated decompression from 450 kPa (c. 35 m depth) (which resulted in barotrauma) and subsequent recompression. Sebastes melanops were s led for liver tissue at days 3, 15 and 31 post-barotrauma. Potential candidate genes were identified from the microarray and then quantitative real-time PCR (qrt-PCR) was used to validate expression levels in biological replicates. Six potential biomarkers associated with the innate immune system were identified that were up-regulated in liver tissue at 3 days post-barotrauma: complement C1q-like protein 2, complement component C3, complement regulatory plasma protein, serum amyloid A-5, c-type lysozyme and hepcidin precursor type I. In addition, complement c1q was correlated to the presence of a ruptured swimbladder, providing further support that this gene may be a good biomarker of injury and recovery. Immune genes were no longer up-regulated at day 31 post-barotrauma, a good indication of recovery in S. melanops.
Publisher: Springer Science and Business Media LLC
Date: 05-05-2021
DOI: 10.1038/S43705-021-00014-2
Abstract: The ability to preserve microbial communities in faecal s les is essential as increasing numbers of studies seek to use the gut microbiome to identify biomarkers of disease. Here we use shotgun metagenomics to rigorously evaluate the technical and compositional reproducibility of five room temperature (RT) microbial stabilisation methods compared to the best practice of flash-freezing. These methods included RNALater, OMNIGene-GUT, a dry BBL swab, LifeGuard, and a novel method for preserving faecal s les, a Copan FLOQSwab in an active drying tube (FLOQSwab-ADT). Each method was assessed using six replicate faecal s les from five participants, totalling 180 s les. The FLOQSwab-ADT performed best for both technical and compositional reproducibility, followed by RNAlater and OMNIgene-GUT. LifeGuard and the BBL swab had unpredictable outgrowth of Escherichia species in at least one replicate from each participant. We further evaluated the FLOQSwab-ADT in an additional 239 s les across 10 in iduals after storage at −20 °C, RT, and 50 °C for four weeks compared to fresh controls. The FLOQSwab-ADT maintained its performance across all temperatures, indicating this method is an excellent alternative to existing RT stabilisation methods.
Publisher: Northwest Scientific Association
Date: 06-2007
Location: United States of America
Location: United States of America
Location: United States of America
Start Date: 2008
End Date: 2010
Funder: National Oceanic and Atmospheric Administration
View Funded ActivityStart Date: 2012
End Date: 2013
Funder: California Sea Grant, University of California
View Funded ActivityStart Date: 2008
End Date: 2008
Funder: National Science Foundation
View Funded Activity