ORCID Profile
0000-0002-9822-9385
Current Organisation
Badan Riset dan Inovasi Nasional Republik Indonesia
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Publisher: Veterinary World
Date: 07-2023
DOI: 10.14202/VETWORLD.2023.1477-1488
Abstract: Background and Aim: Herbal plants have the potential to reduce the population of metagonic bacteria and protozoa due to the bioactive compound contained in herbal plants. This study aimed to evaluate the effect of herbal plant supplementation on rumen fermentation characteristics, methane (CH4) gas emissions, in vitro nutrient digestibility, and protozoan populations. Materials and Methods: This study consisted of two stages. Stage I involved determining the potential of herbal plants to increase total gas production (Orskov and McDonald methods) and reduce the protozoan population (Hristov method). Three potential herbs were selected at this stage and used in Stage II as supplements in the palm kernel cake (PKC)-based diet (30% herbal plants + 70% PKC). Proximate and Van Soest analyses were used to determine the chemical composition. In vitro dry matter digestibility (IVDMD), organic matter (IVOMD), and rumen fermentation characteristics were determined using Theodorous method. Conway microdiffusion was used to determine ammonia concentration (NH3). Gas chromatography was used to determine the total and partial volatile fatty acid production. Results: The results of the first stage showed that seven herbal plants (Moringa oleifera, Rhodomyrtus tomentosa, Clerodendron serratum, Curcuma longa Linn., Urena lobata, Uncaria, and Parkia timoriana) significantly differed in terms of total gas production (p 0.05). Herbal plants can increase gas production and reduce protozoan populations. The highest total gas production was observed using P. timoriana, M. oleifera, and C. longa Linn. Moringa oleifera plants were the most effective in lowering protozoa population. In Stage 2, the supplementation of herbal plants in PKC-based-diet significantly increased IVDMD, that was ranged from 56.72% to 65.77%, IVOMD that was ranged from 52.10% to 59.54%, and NH3, that was ranged from 13.20 mM to 17.91 mM. Volatile fatty acid partial and total gas production potential and CH4 gas emissions were also significantly different from those of the control (p 0.05). Conclusion: Supplementation of M. oleifera, C. longa Linn., and P. timoriana in ruminant diet effectively increased total gas production, IVDMD percentage, and IVOMD, and reduced CH4 gas emissions and protozoa populations during rumen fermentation. Keywords: herbal plants, in vitro, methane, protozoa.
Publisher: University of Queensland Library
Date: 2019
Publisher: MDPI AG
Date: 21-12-2022
Abstract: Bactericidal ermeability-increasing protein, a primary factor of the innate immune system of mammals, participates in natural immune protection against invading bacteria. BPIFA1 actively contributes to host defense via multiple mechanisms, such as antibacterial, surfactant, airway surface liquid control, and immunomodulatory activities. However, the evolutionary history and selection forces on the BPIFA1 gene in mammals during adaptive evolution are poorly understood. This study examined the BPIFA1 gene of humans compared with that of other mammalian species to estimate the selective pressure derived by adaptive evolution. To assess whether or not positive selection occurred, we employed several different possibility tests (M1 vs. M2 and M7 vs. M8). The proportions of positively selected sites were significant, with a likelihood log value of 93.63 for the BPIFA1 protein. The Selecton server was used on the same dataset to reconfirm positive selection for specific sites by employing the Mechanistic-Empirical Combination model, thus providing additional evidence supporting the findings of positive selection. There was convincing evidence for positive selection signals in the BPIFA1 genes of mammalian species, which was more significant for selection signs and creating signals. We performed probability tests comparing various models based on dN/dS ratios to recognize specific codons under positive selection pressure. We identified positively selected sites in the LBP-BPI domain of BPIFA1 proteins in the mammalian genome, including a lipid-binding domain with a very high degree of selectivity for DPPC. BPIFA1 activates the upper airway’s innate immune system in response to numerous genetic signals in the mammalian genome. These findings highlight evolutionary advancements in immunoregulatory effects that play a significant role in the antibacterial and antiviral defenses of mammalian species.
Publisher: EJournal Publishing
Date: 2016
Publisher: Elsevier BV
Date: 03-2023
Publisher: Springer Science and Business Media LLC
Date: 29-06-2023
Publisher: IOP Publishing
Date: 12-2022
DOI: 10.1088/1755-1315/1114/1/012068
Abstract: Environmentally friendly products are currently a concern for the community to consume. The increase in livestock products is also in line with the increasing need for feed. Some chemicals materials are used in producing feed have been prohibited from being used. Organic binder for pellet feed is one of the solutions to replace these non-organic materials. In this study, two dosages of molasses and bentonite as organic pellet binders were tested on the quality of pellet feed with factorial arrangement. The parameter was physic quality pellet such as hardness, durability, friction and density. Chemical component of pellet product such as dry matter, ash, protein, fat and fibre. Organic binder of 5% molasses and 5% bentonite were optimum to obtain better physical pellet condition. In addition, 5% of molasses binder resulted in highest pellet durability index (93.40%). As conclusion, molasses and bentonite are suggested as organic binders for producing good quality pellet feed.
Location: Indonesia
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