ORCID Profile
0000-0002-3665-5717
Current Organisation
The University of Texas at Austin
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Publisher: eLife Sciences Publications, Ltd
Date: 06-12-2017
DOI: 10.7554/ELIFE.30051
Abstract: Pathogens rely on proteins embedded on their surface to perform tasks essential for host infection. These obligatory structures exposed to the host immune system provide important targets for rational vaccine design. Here, we use a systematically designed series of multi-domain constructs in combination with small angle X-ray scattering (SAXS) to determine the structure of the main immunoreactive region from a major antigen from Leptospira interrogans, LigB. An anti-LigB monoclonal antibody library exhibits cell binding and bactericidal activity with extensive domain coverage complementing the elongated architecture observed in the SAXS structure. Combining antigenic motifs in a single-domain chimeric immunoglobulin-like fold generated a vaccine that greatly enhances leptospiral protection over vaccination with single parent domains. Our study demonstrates how understanding an antigen’s structure and antibody accessible surfaces can guide the design and engineering of improved recombinant antigen-based vaccines.
Publisher: Public Library of Science (PLoS)
Date: 19-12-2014
Publisher: American Chemical Society (ACS)
Date: 28-06-2013
DOI: 10.1021/BI400128R
Abstract: Many host-parasite interactions are mediated via surface-exposed proteins containing bacterial immunoglobulin-like (Big) domains. Here, we utilize the spectral properties of a conserved Trp to provide evidence that, along with a Phe, these residues are positioned within the hydrophobic core of a subset of Big_2 domains. The mutation of the Phe to Ala decreases Big_2 domain stability and impairs the ability of LigBCen2 to bind to the host protein, fibronectin.
Publisher: Public Library of Science (PLoS)
Date: 30-10-2015
Publisher: American Chemical Society (ACS)
Date: 04-08-2014
DOI: 10.1021/BI500669U
Publisher: Public Library of Science (PLoS)
Date: 13-09-2016
Publisher: American Chemical Society (ACS)
Date: 11-2013
DOI: 10.1021/CB4007166
Publisher: Frontiers Media SA
Date: 09-05-2017
Publisher: Elsevier BV
Date: 09-2013
Publisher: Elsevier BV
Date: 02-2013
No related grants have been discovered for Ching-Lin Hsieh.