ORCID Profile
0000-0002-1799-1336
Current Organisation
Badan Riset dan Inovasi Nasional Republik Indonesia
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Publisher: F1000 Research Ltd
Date: 19-01-2021
DOI: 10.12688/F1000RESEARCH.27952.1
Abstract: Background: Breast cancer is the most common cancer in women worldwide and is the leading cause of death in women with cancer. One novel therapy used for breast cancer treatment is non-contact electric fields called electro-capacitive cancer therapy (ECCT) with intermediate frequency (100 kHz) and low intensity (18 Vpp). The objective of this study was to examine the effect of ECCT on mammary tumors growth in rats and observing the immune responses that play a role in fighting the tumor. Methods: Female SD rats were used and ided into four groups, namely control (NINT), placebo (NIT), non- therapy (INT), and therapy (IT) groups with 6 biological replicates in each group. Rats in INT and IT groups were treated with 7,12-dimethylbenz[a]anthracene for mammary tumor induction. Only rats in NIT and IT groups were exposed to ECCT in idually for 10 hours per day for 21 days. The size of all tumors was measured with a digital caliper. The distributions of PCNA, ErbB2, caspase-3, CD68, CD4 and CD8-positive cells were observed with immunohistochemistry and scoring with ImageJ. Results: The growth rate of mammary tumors in IT group was significantly lower (p .05) than that in the INT group. The number of mitotic figures and the percentage of PCNA, caspase-3, and CD68- positive cells in IT group were significantly lower (p .05) than those in INT group. Conversely, the percentage of CD8-positive T cells in IT group was significantly higher (p .05) than that in INT group. Moreover, the CD4/CD8 ratio in IT group was decreased. Some tumor tissues were blackened and detached from the surrounding tissue, resulting in an open wound which then healed up upon exposure. Conclusions: Non-contact electric fields exposure showed inhibition on mammary tumor growth in rats while inducing CD8+ T cells that lead to tumor cells death and potentially helps wound healing.
Publisher: F1000 Research Ltd
Date: 04-05-2021
DOI: 10.12688/F1000RESEARCH.27952.2
Abstract: Background: Breast cancer is the most common cancer in women worldwide and is the leading cause of death amongst women with cancer. One novel therapy used for breast cancer treatment constitutes non-contact electric fields and is called electro-capacitive cancer therapy (ECCT) with intermediate frequency and low intensity. The objective of this study was to examine the effect of ECCT on mammary tumors growth in rats and observing the immune responses that play a role in fighting the tumor. Methods: Female SD rats were used and ided into four groups, namely control (NINT), placebo (NIT), non- therapy (INT), and therapy (IT) groups with 6 biological replicates in each group. Rats in INT and IT groups were treated with 7,12-dimethylbenz[a]anthracene for mammary tumor induction. Only rats in NIT and IT groups were exposed to ECCT in idually for 10 hours per day for 21 days. The size of all tumors was measured with a digital caliper. The distributions of PCNA, ErbB2, caspase-3, CD68, CD4, and CD8-positive cells were observed with immunohistochemistry and scoring with ImageJ. Results: The growth rate of mammary tumors in IT group was significantly lower (p .05) than that in INT group. The number of mitotic figures and the percentage of PCNA, caspase-3, and CD68-positive cells in IT group were significantly lower (p .05) than those in INT group. Conversely, the percentage of CD8-positive T cells in IT group was significantly higher (p .05) than that in INT group. Moreover, the CD4/CD8 ratio in IT group was found to have decreased. Some tumor tissues were blackened and detached from the surrounding tissue, resulting in an open wound which then healed upon exposure. Conclusions: Non-contact electric fields exposure showed inhibition on mammary tumor growth in rats while inducing CD8+ T cells, leading to tumor cell death and potentially helping wounds heal.
Publisher: F1000 Research Ltd
Date: 25-05-2023
DOI: 10.12688/F1000RESEARCH.110080.2
Abstract: Background: A novel modality of cancer treatment based on exposure to non-contact electric fields has been developed to reduce the cancer incidence. However, the safety of this electric field exposure on vital organs has not been fully investigated. Therefore, the purpose of this study was to observe the safety of the electric field exposure on kidney and liver structures. Methods: Female Sprague-Dawley rats were ided into one control group and three treatment groups. Animals were treated with 7,12-dimethylbenz[a]anthracene for mammary tumour induction and exposed to non-contact electric fields in idually for 10 hours a day for three weeks. Fresh s les of kidney and liver were collected for observation of structural damage in both organs. Both organs were prepared for histopathological cross-sectioning using the paraffin method and Hematoxylin & Eosin staining followed by histological scoring using the post-examination masking method. Results: Damages found in the kidney were as follows: thickening of Bowman capsule, karyolysis, karyorrexhis, pyknosis, cloudy swelling, epithelial sloughing, inflammation, haemorrhage, and congestion. The thickening of Bowman’s capsule was significant, but other damages to the rest of kidney tissue were not significant. In addition, the number of inflammations and haemorrhages in the kidney structure of the placebo group was the lowest and significantly different compared to the other three groups. All damages to the kidneys were also found in the liver, but each showed a different degree of damage. The damages to the liver caused by exposure to the electric fields were not significant. Conclusions: Together with the effects of DMBA administration, exposure to non-contact electric fields can cause thickening of Bowman's capsule in the glomerulus of the kidney, however, in other kidney tissues and in the liver, exposure to this electric field was safe. It can even decrease the number of inflammations and haemorrhages in the organs.
Publisher: F1000 Research Ltd
Date: 02-2023
DOI: 10.12688/F1000RESEARCH.110080.1
Abstract: Background: There were an estimated 2.1 million breast cancer diagnoses in 2018 worldwide, which is about 11.6% of the total cancer incidence. A novel modality of cancer treatment based on exposure to non-contact electric fields has been developed to reduce cancer incidence. However, the safety of the electric field exposure was not fully investigated. Therefore, the purpose of this study is to observe the safety of the electric field exposure on renal and liver structure. Methods: Female Sprague-Dawley rats were ided into one control group and three treatment groups. Animals were treated with 7,12-dimethylbenz[a]anthracene for mammary tumour induction and exposed to non-contact electric fields in idually for 10 hours a day for three weeks. Fresh s les of the kidney and liver were collected for observing structural damage in both organs. The two organs were prepared for histopathological cross-sectioning using the paraffin method and Hematoxylin & Eosin staining followed by histological scoring using the post-examination masking method. Results: The damages found in the kidney were the following: thickening of Bowman capsule, karyolysis, karyorrexhis, pyknosis, cloudy swelling, epithelial sloughing, inflammation, haemorrhage, and congestion. The number of inflammation and haemorrhage in the kidney structure of the placebo group was the lowest and significantly different from the three other groups. All damages in the kidney were also found in the liver, but each showed different levels of damage. The damages in the kidney and liver caused by the exposure were not significant. Conclusions: The non-contact electric fields were not harmful to renal and liver structure in tumour-induced rats. Instead, it may increase the renal function in normal rats.
Publisher: F1000 Research Ltd
Date: 26-10-2023
Location: Indonesia
No related grants have been discovered for Nisrina Firdausi.