ORCID Profile
0000-0002-7649-3134
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Publisher: Cold Spring Harbor Laboratory
Date: 07-05-2022
DOI: 10.1101/2022.05.06.490935
Abstract: Cell types are the fundamental building blocks of metazoan bio ersity and offer a powerful perspective for inferring evolutionary phenomena. With the development of single-cell transcriptomic techniques, new definitions of cell types are emerging. This allows a conceptual reassessment of traditional definitions of novel cell types and their evolution. Research in echinoderms, particularly sea star and sea urchin embryos have contributed significantly to understanding the evolution of novel cell types, in particular the primary mesenchyme cells (PMCs) and pigment cells that are found in sea urchin but not sea star embryos. This paper outlines the development of a gene expression atlas for the bat star, Patiria miniata , using single nuclear RNA sequencing (snRNA-seq) of embryonic stages. The atlas revealed 22 cell clusters covering all expected cell types from the endoderm, mesoderm and ectoderm germ layers. In particular, four distinct neural clusters, an immune cluster, and distinct right and left coelom clusters were revealed as distinct cell states. A comparison with Strongylocentrotus purpuratus embryo single cell transcriptomes was performed using 1:1 orthologs to anchor and then compare gene expression patterns. S. purpuratus primordial germ cell equivalents were not detected in P. minata , while the left coelom of P. miniata has no equivalent cell cluster in S. purpuratus . Pigment cells of S. purpuratus map to clusters containing immune mesenchyme and neural cells of P. miniata , while the PMCs of S. purpuratus are revealed as orthologous to the right coelom cluster of P. miniata . These results suggest a new interpretation of the evolution of these well-studied cell types and a reflection on the definition of novel cell types.
Publisher: eLife Sciences Publications, Ltd
Date: 11-05-2023
Publisher: eLife Sciences Publications, Ltd
Date: 20-07-2023
DOI: 10.7554/ELIFE.80090
Abstract: Cell types are the building blocks of metazoan bio ersity and offer a powerful perspective for inferring evolutionary phenomena. With the development of single-cell transcriptomic techniques, new definitions of cell types are emerging. This allows a conceptual reassessment of traditional definitions of novel cell types and their evolution. Research in echinoderms, particularly sea star and sea urchin embryos has contributed significantly to understanding the evolution of novel cell types, through the examination of skeletogenic mesenchyme and pigment cells, which are found in sea urchin larvae, but not sea star larvae. This paper outlines the development of a gene expression atlas for the bat sea star, Patiria miniata , using single nuclear RNA sequencing (snRNA-seq) of embryonic stages. The atlas revealed 23 cell clusters covering all expected cell types from the endoderm, mesoderm, and ectoderm germ layers. In particular, four distinct neural clusters, an immune-like cluster, and distinct right and left coelom clusters were revealed as distinct cell states. A comparison with Strongylocentrotus purpuratus embryo single-cell transcriptomes was performed using 1:1 orthologs to anchor and then compare gene expression patterns. The equivalent of S. purpuratus piwil3+ Cells were not detected in P. miniata , while the Left Coelom of P. miniata has no equivalent cell cluster in S. purpuratus . These differences may reflect changes in developmental timing between these species. While considered novel morphologically, the Pigment Cells of S. purpuratus map to clusters containing Immune-like Mesenchyme and Neural cells of P. miniata , while the Skeletogenic Mesenchyme of S. purpuratus are revealed as orthologous to the Right Coelom cluster of P. miniata . These results suggest a new interpretation of the evolution of these well-studied cell types and a reflection on the definition of novel cell types.
No related grants have been discovered for Cheryl Anne Telmer.