ORCID Profile
0000-0001-7124-5812
Current Organisations
University of Tsukuba
,
Hokkaido University
,
Flinders University
,
National Institute of Advanced Industrial Science and Technology
,
Nagaoka University of Technology
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Publisher: Microbiology Society
Date: 07-2003
Abstract: A phylogenetically novel aerobic bacterium was isolated from an anaerobic-aerobic sequential batch reactor operated under enhanced biological phosphorus removal conditions for wastewater treatment. The isolation strategy used targeted slowly growing polyphosphate-accumulating bacteria by combining low-speed centrifugations and prolonged incubation on a low-nutrient medium. The isolate, designated strain T-27T, was a gram-negative, rod-shaped aerobe. Cells often appeared to ide by budding replication. Strain T-27T grew at 25-35 degrees C with an optimum growth temperature of 30 degrees C, whilst no growth was observed below 20 degrees C or above 37 degrees C within 20 days incubation. The pH range for growth was 6.5-9.5, with an optimum at pH 7.0. Strain T-27T was able to utilize a limited range of substrates, such as yeast extract, polypepton, succinate, acetate, gelatin and benzoate. Neisser staining was positive and 4,6-diamidino-2-phenylindole-stained cells displayed a yellow fluorescence, indicative of polyphosphate inclusions. Menaquinone 9 was the major respiratory quinone. The cellular fatty acids of the strain were mainly composed of iso-C15:0, C16:1 and C14:0. The G + C content of the genomic DNA was 66 mol%. Comparative analyses of 16S rRNA gene sequences indicated that strain T-27T belongs to candidate ision BD (also called KS-B), a phylum-level lineage in the bacterial domain, to date comprised exclusively of environmental 16S rDNA clone sequences. Here, a new genus and species are proposed, Gemmatimonas aurantiaca (type strain T-27T=JCM 11422T=DSM 14586T) gen. nov., sp. nov., the first cultivated representative of the Gemmatimonadetes phyl. nov. Environmental sequence data indicate that this phylum is widespread in nature and has a phylogenetic breadth (19% 16S rDNA sequence ergence) that is greater than well-known phyla such as the Actinobacteria (18% ergence).
Publisher: Elsevier BV
Date: 12-2021
Publisher: Elsevier BV
Date: 12-2020
Publisher: American Society for Microbiology
Date: 03-2006
DOI: 10.1128/AEM.72.3.2080-2091.2006
Abstract: The classical perception of members of the gram-positive Desulfotomaculum cluster I as sulfate-reducing bacteria was recently challenged by the isolation of new representatives lacking the ability for anaerobic sulfate respiration. For ex le, the two described syntrophic propionate-oxidizing species of the genus Pelotomaculum form the novel Desulfotomaculum subcluster Ih. In the present study, we applied a polyphasic approach by using cultivation-independent and culturing techniques in order to further characterize the occurrence, abundance, and physiological properties of subcluster Ih bacteria in low-sulfate, methanogenic environments. 16S rRNA (gene)-based cloning, quantitative fluorescence in situ hybridization, and real-time PCR analyses showed that the subcluster Ih population composed a considerable part of the Desulfotomaculum cluster I community in almost all s les examined. Additionally, five propionate-degrading syntrophic enrichments of subcluster Ih bacteria were successfully established, from one of which the new strain MGP was isolated in coculture with a hydrogenotrophic methanogen. None of the cultures analyzed, including previously described Pelotomaculum species and strain MGP, consumed sulfite, sulfate, or organosulfonates. In accordance with these phenotypic observations, a PCR-based screening for dsrAB (key genes of the sulfate respiration pathway encoding the alpha and beta subunits of the dissimilatory sulfite reductase) of all enrichments/(co)cultures was negative with one exception. Surprisingly, strain MGP contained dsrAB , which were transcribed in the presence and absence of sulfate. Based on these and previous findings, we hypothesize that members of Desulfotomaculum subcluster Ih have recently adopted a syntrophic lifestyle to thrive in low-sulfate, methanogenic environments and thus have lost their ancestral ability for dissimilatory sulfate/sulfite reduction.
Publisher: Elsevier BV
Date: 11-2018
Publisher: Springer Science and Business Media LLC
Date: 23-01-2015
Publisher: Elsevier BV
Date: 2019
Publisher: American Society for Microbiology
Date: 11-2003
DOI: 10.1128/AEM.69.11.6808-6815.2003
Abstract: The ecology of the uncultured, but large and morphologically conspicuous, rumen bacterium Oscillospira spp. was studied. Oscillospira- specific 16S rRNA gene sequences were detected in North American domestic cattle, sheep from Australia and Japan, and Norwegian reindeer. Phylogenetic analysis of the sequences obtained allowed definition of three operational taxonomic units within the Oscillospira clade. Consistent with this genetic ersity, we observed atypical smaller morphotypes by using an Oscillospira- specific fluorescence in situ hybridization probe. Despite the visual disappearance of typical large Oscillospira morphotypes, the presence of Oscillospira spp. was still detected by Oscillospira- specific PCR in the rumen of cattle and sheep. These observations suggest the broad presence of Oscillospira species in various rumen ecosystems with the level, and most likely the morphological form, dependent on diet. An ecological analysis based on enumeration of the morphologically conspicuous, large-septate form confirms that the highest counts are associated with the feeding of fresh forage diets to cattle and sheep and in two different subspecies of reindeer investigated.
Publisher: Thomas Telford Ltd.
Date: 05-2020
Abstract: The aim of this study was to develop sustainable concrete using waste products to reduce both the carbon dioxide emissions associated with concrete production and the extraction of non-renewable natural resources. The development of the new sustainable concrete involved the replacement of cement with industrial by-products (fly ash, glass powder and ground granulated blast-furnace slag (GGBS)) and the replacement of natural river sand (NS) with waste-based sand (lead-smelter slag (LSS)). Twenty-four batches of mortar mixes were produced and tests were performed to determine the flowability, compressive strength and direct tensile strength of each batch. Microstructural analysis was undertaken to explain the experimentally obtained properties of the mortars. The compressive and tensile strengths of waste-based mortars containing LSS were found to be similar to those of mortars containing NS. Mortars with 80% replacement of cement with GGBS and 100% replacement of NS with LSS showed minimal strength reduction (4%) compared with the conventional mortar. The strength reductions of the waste-based mortars compared with the conventional mortar increased at 90% and 100% cement replacement levels, but remained limited to approximately 20% (at 90% GGBS) and 30% (at 100% GGBS). The findings of this study are promising and point to the potential development of new structural-grade mortars using full or near-full replacement of cement with industrial by-products and full replacement of NS with waste-based sand.
Location: Japan
No related grants have been discovered for Yoichi Kamagata.