ORCID Profile
0000-0001-5457-7030
Current Organisation
Nanyang Technological University
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Publisher: Wiley
Date: 29-01-2022
Abstract: A critical challenge of nanoparticle‐based imaging diagnosis of cancers is limited accumulation of nanoparticles at the biological target. Here, a cancer cell derived membrane coated 2D layered double hydroxides nanoparticle (CM‐PEG/MnLDH) has been developed to realize both cell‐specific targeting and deep tissue penetration for enhanced magnetic resonance imaging (MRI) of tumors. Using 4T1 breast cancer cells as a model, the biomimetic CM‐PEG/MnLDH exhibited excellent cell‐specific targeting ability with significantly higher accumulation in homologous cancer cells than the nonhomologous cells. Using 3D tumor spheroid cultures, the CM‐PEG/MnLDH demonstrated highly selective binding and deep tissue penetration ability. In vivo imaging revealed that intravenous injection of CM‐PEG/MnLDH provides clear MRI of tumor tissues in a 4T1 mouse model. Particularly, the CM‐PEG/MnLDH showed enhanced imaging performance in the central region of tumors, owing to the cell homing and tissue penetration ability of the biomimetic nanoparticle. In vivo biosafety evaluation of the CM‐PEG/MnLDH showed a high degree of biocompatibility, signifying its suitability for further clinical applications. The biomimetic CM‐PEG/MnLDH nanoparticle featured with cell‐specific targeting ability and deep tissue penetration is a promising MRI contrast agent for precise cancer diagnosis.
Publisher: The Optical Society
Date: 02-08-2018
DOI: 10.1364/AO.57.006687
Publisher: SPIE-Intl Soc Optical Eng
Date: 02-06-2020
Publisher: Elsevier BV
Date: 03-2021
Publisher: Wiley
Date: 22-02-2023
Abstract: Multicellular spheroids and organoids are promising in vitro 3D models in personalized medicine and drug screening. They replicate the structural and functional characteristics of human organs in vivo. Microfluidic technology and micro‐nano fabrication can fulfill the high requirement of the engineering approach in the growing research interest in spheroids and organoids. In this review, spheroids and organoids are comparatively introduced. Then it is illustrated how spheroids‐ and organoids‐on‐a‐chip technology facilitates their establishment, expansion, and application through spatial‐temporal control, mechanical cues modeling, high‐throughput analysis, co‐culture, multi‐tissue interactions, biosensing, and bioimaging integration. The potential opportunities and challenges in developing spheroids‐ and organoids‐on‐a‐chip technology are finally outlooked.
Publisher: American Chemical Society (ACS)
Date: 29-10-2021
Abstract: Microrobots can expand our abilities to access remote, confined, and enclosed spaces. Their potential applications inside our body are obvious,
Publisher: Optica Publishing Group
Date: 24-09-2021
DOI: 10.1364/OE.438258
Abstract: This paper demonstrates, for the first time, a novel demodulation technique that can be applied for interrogating a shortest cavity in multi-cavity Fabry–Pérot (F–P) sensors. In this demodulation technique, using an lified spontaneous emission (ASE) light source and two optical fiber broadband filters, the interference only occurs in a shortest F–P cavity that is shorter than the half of the coherence length. Using a signal calibration algorithm, two low-coherence interference optical signals with similar coherence lengths were calibrated to obtain two quadrature signals. Then, the change in the cavity length of the shortest F–P cavity was interrogated by the two quadrature signals and the arctangent algorithm. The experimental results show that the demodulation technique successfully extracted 1 kHz and 500 Hz vibration signals with 39.28 µm and 64.84 µm initial cavity lengths, respectively, in a multi-cavity F–P interferometer. The demodulation speed is up to 500 kHz, and the demodulation technique makes it possible for multi-cavity F–P sensors to measure dynamic and static parameters simultaneously. The results show that the demodulation technique has wide application potential in the dynamic measurement of multi-cavity F–P sensors.
Publisher: Elsevier BV
Date: 06-2017
Publisher: Elsevier BV
Date: 07-2016
Publisher: Elsevier BV
Date: 07-2018
Publisher: Wiley
Date: 14-01-2020
Abstract: Cancer spheroids have structural, functional, and physiological similarities to the tumor, and have become a low-cost in vitro model to study the physiological responses of single cells and therapeutic efficacy of drugs. However, the tiny spheroid, made of a cluster of high-density cells, is highly scattering and absorptive, which prevents light microscopy techniques to reach the depth inside spheroids with high resolution. Here, a method is reported for super-resolution mapping of single nanoparticles inside a spheroid. It first takes advantage of the self-healing property of a "nondiffractive" doughnut-shaped Bessel beam from a 980 nm diode laser as the excitation, and further employs the nonlinear response of the 800 nm emission from upconversion nanoparticles, so that both excitation and emission at the near-infrared can experience minimal loss through the spheroid. These strategies lead to the development of a new nanoscopy modality with a resolution of 37 nm, 1/26th of the excitation wavelength. This method enables mapping of single nanoparticles located 55 µm inside a spheroid, with a resolution of 98 nm. It suggests a solution to track single nanoparticles and monitor their release of drugs in 3D multicellar environments.
Publisher: Wiley
Date: 08-09-2021
Abstract: Mammary tumor organoids have become a promising in vitro model for drug screening and personalized medicine. However, the dependency on the basement membrane extract (BME) as the growth matrices limits their comprehensive application. In this work, mouse mammary tumor organoids are established by encapsulating tumor pieces in non‐adhesive alginate. High‐throughput generation of organoids in alginate microbeads is achieved utilizing microfluidic droplet technology. Tumor pieces within the alginate microbeads developed both luminal‐ and solid‐like structures and displayed a high similarity to the original fresh tumor in cellular phenotypes and lineages. The mechanical forces of the luminal organoids in the alginate capsules are analyzed with the theory of the thick‐wall pressure vessel (TWPV) model. The luminal pressure of the organoids increase with the lumen growth and can reach 2 kPa after two weeks’ culture. Finally, the mammary tumor organoids are treated with doxorubicin and latrunculin A to evaluate their application as a drug screening platform. It is found that the drug response is related to the luminal size and pressures of organoids. This high‐throughput culture for mammary tumor organoids may present a promising tool for preclinical drug target validation and personalized medicine.
Publisher: Frontiers Media SA
Date: 30-11-2021
Abstract: Over 90% of potential anti-cancer drug candidates results in translational failures in clinical trials. The main reason for this failure can be attributed to the non-accurate pre-clinical models that are being currently used for drug development and in personalised therapies. To ensure that the assessment of drug efficacy and their mechanism of action have clinical translatability, the complexity of the tumor microenvironment needs to be properly modelled. 3D culture models are emerging as a powerful research tool that recapitulates in vivo characteristics. Technological advancements in this field show promising application in improving drug discovery, pre-clinical validation, and precision medicine. In this review, we discuss the significance of the tumor microenvironment and its impact on therapy success, the current developments of 3D culture, and the opportunities that advancements that in vitro technologies can provide to improve cancer therapeutics.
Publisher: SPIE
Date: 19-10-2016
DOI: 10.1117/12.2247037
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D1TC05782K
Abstract: The optical photographs and reaction mechanism of self-catalysis synthesis of R-CDs by a solvent-free method.
Publisher: MDPI AG
Date: 28-03-2018
DOI: 10.3390/S18041011
Publisher: SPIE
Date: 20-08-2020
DOI: 10.1117/12.2569754
Publisher: Elsevier BV
Date: 02-2022
Publisher: Springer Science and Business Media LLC
Date: 23-06-2016
DOI: 10.1007/S13320-016-0327-7
Abstract: A miniature fiber-optic Fabry-Perot interferometer (MOFPI) fabricated by splicing a hollow silica tube (HST) with inner diameter of 4 µm to the end of a single-mode fiber is investigated and experimentally demonstrated. The theoretical relationship between the free spectrum range and the length of HST is verified by fabricating several MOFPIs with different lengths. We characterize the MOFPIs for temperature, liquid refractive index, and strain. Experimental results show that the sensitivities of the temperature, liquid refractive index, and strain are 16.42 pm/°C,–118.56 dB/RIU, and 1.21 pm/µε, respectively.
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9LC00872A
Abstract: Gradient-sized spheroids can be simultaneously generated on a single chip using a liquid-dome method assisted by the surface tension. The facile method can be used for investigation of the size-dependent behaviors of spheroids in biomedical research.
Publisher: Wiley
Date: 25-05-2022
Abstract: To fully investigate cellular responses to stimuli and perturbations within tissues, it is essential to replicate the complex molecular interactions within the local microenvironment of cellular niches. Here, the authors introduce Alginate‐based tissue engineering (ALTEN), a biomimetic tissue platform that allows ex vivo analysis of explanted tissue biopsies. This method preserves the original characteristics of the source tissue's cellular milieu, allowing multiple and erse cell types to be maintained over an extended period of time. As a result, ALTEN enables rapid and faithful characterization of perturbations across specific cell types within a tissue. Importantly, using single‐cell genomics, this approach provides integrated cellular responses at the resolution of in idual cells. ALTEN is a powerful tool for the analysis of cellular responses upon exposure to cytotoxic agents and immunomodulators. Additionally, ALTEN's scalability using automated microfluidic devices for tissue encapsulation and subsequent transport, to enable centralized high‐throughput analysis of s les gathered by large‐scale multicenter studies, is shown.
Publisher: MDPI AG
Date: 20-01-2017
DOI: 10.3390/S17010196
Publisher: The Optical Society
Date: 02-03-2018
DOI: 10.1364/AO.57.001738
No related grants have been discovered for Guocheng Fang.