ORCID Profile
0000-0001-5999-1310
Current Organisations
The University of Edinburgh
,
Technische Universität Berlin
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Publisher: Cold Spring Harbor Laboratory
Date: 26-09-2018
DOI: 10.1101/424697
Abstract: The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein-protein interactions has largely increased during the last years. As the technique is now becoming routine for in vitro and in vivo applications in proteomics and structural biology there is a pressing need to define protocols as well as data analysis and reporting formats that are generally accepted in the field and that have shown to lead to high-quality results. This first, community-based harmonization study on XL-MS is based on the results of 32 groups participating worldwide. The aim of this paper is to summarize the status quo of XL-MS and to compare and evaluate existing cross-linking strategies. From the results obtained, common protocols will be established. Our study serves as basis for establishing best practice guidelines in the field for conducting cross-linking experiments, performing data analysis, and reporting formats with the ultimate goal of assisting scientists to generate accurate and reproducible XL-MS results.
Publisher: Elsevier BV
Date: 11-2020
Publisher: Springer Science and Business Media LLC
Date: 17-02-2020
Publisher: Elsevier BV
Date: 07-2015
Publisher: Springer Science and Business Media LLC
Date: 13-02-2017
DOI: 10.1038/NSMB.3372
Publisher: Springer Science and Business Media LLC
Date: 24-11-2016
DOI: 10.1038/NCOMMS13595
Abstract: Little is known about how archaeal viruses perturb the transcription machinery of their hosts. Here we provide the first ex le of an archaeo-viral transcription factor that directly targets the host RNA polymerase (RNAP) and efficiently represses its activity. ORF145 from the temperate Acidianus two-tailed virus (ATV) forms a high-affinity complex with RNAP by binding inside the DNA-binding channel where it locks the flexible RNAP cl in one position. This counteracts the formation of transcription pre-initiation complexes in vitro and represses abortive and productive transcription initiation, as well as elongation. Both host and viral promoters are subjected to ORF145 repression. Thus, ORF145 has the properties of a global transcription repressor and its overexpression is toxic for Sulfolobus . On the basis of its properties, we have re-named ORF145 RNAP Inhibitory Protein (RIP).
Publisher: Springer Science and Business Media LLC
Date: 09-05-2022
Publisher: Springer Science and Business Media LLC
Date: 09-05-2022
Publisher: Springer Science and Business Media LLC
Date: 09-2022
Publisher: American Society of Hematology
Date: 09-02-2022
Abstract: The mechanisms of coordinated changes in proteome composition and their relevance for the differentiation of neutrophil granulocytes are not well studied. Here, we discover two novel human genetic defects in SRPRA and SRP19, constituents of the mammalian co-translational targeting machinery and characterize their role in neutrophil granulocyte differentiation. We systematically study the proteome of neutrophil granulocytes from patients with variants in the signal recognition particle (SRP) genes, HAX1, and ELANE and identify global as well as specific proteome aberrations. Using in vitro differentiation of human induced pluripotent stem cells and in vivo zebrafish models, we study the effects of SRP-deficiency on neutrophil granulocyte development. In a heterologous cell-based inducible protein expression system, we validate the effects conferred by SRP dysfunction for selected proteins that we identified in our proteome screen. Thus, SRP-dependent protein processing, intracellular trafficking and homeostasis are critically important for the differentiation of neutrophil granulocytes.
Publisher: American Chemical Society (ACS)
Date: 02-05-2019
Publisher: Elsevier BV
Date: 04-2019
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Juri Rappsilber.