ORCID Profile
0000-0003-1551-7873
Current Organisations
University of California, Berkeley
,
Nanyang Technological University
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Publisher: Wiley
Date: 08-1998
Publisher: Cold Spring Harbor Laboratory
Date: 25-07-2023
DOI: 10.1101/2023.07.25.550329
Abstract: The coordination between actin and microtubule network is crucial, yet our understanding of the underlying mechanisms remains limited. In this study, we used travelling waves in the cell cortex to characterize the collective dynamics of cytoskeletal networks. Our findings show that Cdc42 and F-BAR-dependent actin waves in mast cells are mainly driven by formin-mediated actin polymerization, with the microtubule-binding formin FH2 domain-containing protein 1 (FHDC1) identified as an early regulator. The depolymerization of microtubules coincides with the nucleation of actin waves, and the concurrent release of FHDC1 from microtubule is required for actin waves. Lastly, we show the importance of the actin-microtubule linkage mediated by FHDC1 in crucial cellular processes such as cell ision and migration. Our data provided molecular insights into the nucleation mechanisms of actin waves and uncover an antagonistic interplay between microtubule and actin polymerization in their collective dynamics.
Publisher: Oxford University Press (OUP)
Date: 07-11-2011
Publisher: Proceedings of the National Academy of Sciences
Date: 08-03-2021
Abstract: Cellulose is the most abundant biopolymer on Earth and has many potential industrial applications, such as renewable energy and sustainable materials. Here we report the apo and UDP-glucose (UDP-Glc)–bound crystal structures of the catalytic domain of Arabidopsis thaliana CESA3. Our results offer a structural basis for how the substrate UDP-Glc and a metal ion, Mn 2+ , which is required for cellulose synthesis, are coordinated in plant CESAs. Furthermore, our structure reveals that CESAs may form homodimers through interactions between specific beta strands that likely aid in the early stages of CESA complex formation.
Publisher: Wiley
Date: 08-06-2007
Publisher: Oxford University Press (OUP)
Date: 30-08-2023
Abstract: The plant cell wall (CW) is one of the most important physical barriers phytopathogens must conquer to invade their hosts. This barrier is a dynamic structure that responds to pathogen infection through a complex network of immune receptors, together with CW-synthesizing and CW-degrading enzymes. Callose deposition in the primary CW is a well-known physical response to pathogen infection. Notably, callose and cellulose biosynthesis share an initial substrate, UDP-glucose, which is the main load-bearing component of the CW. However, how these two critical biosynthetic processes are balanced during plant-pathogen interactions remains unclear. Here, using two different pathogen-derived molecules, bacterial flagellin (flg22) and the diffusible signal factor (DSF) produced by Xanthomonas c estris pv. c estris, we show a negative correlation between cellulose and callose biosynthesis in Arabidopsis (Arabidopsis thaliana). By quantifying the abundance of callose and cellulose under DSF or flg22 elicitation and characterizing the dynamics of the enzymes involved in the biosynthesis and degradation of these two polymers, we show that the balance of these two CW components is mediated by the activity of a β-1,3-glucanase (BG2). Our data demonstrate balanced cellulose and callose biosynthesis during plant immune responses.
Publisher: MDPI AG
Date: 16-01-2023
DOI: 10.3390/JPM13010160
Abstract: We aim to delineate whether there is increased blood loss with the use of cannulated pedicle screws compared to solid pedicle screws in patients undergoing posterior spinal fusion. A single-centre retrospective case-control study was undertaken on patients undergoing PSF for spinal fractures. Cannulated screw fixation was compared with solid screw fixation. Intraoperative blood loss was estimated using pre and postoperative haemoglobin levels, recorded estimated blood loss and cell saver reports. Anticoagulation, blood product administration, operative time and number of levels fused were assessed. A total of 64 cases, 32 in each cohort, were included in the analysis. Overall mean haemoglobin reduction from pre- to post-operative was 2.82 ± 1.85 g/L per screw inserted in the cannulated group, compared to a haemoglobin decrease of 2.81 ± 1.521 g/L per screw inserted in the solid screw group (p = 0.971). Total estimated intraoperative blood loss was 616.3 + 355.4 mL in the cannulated group, compared to 713.6 + 473.5 mL in the solid screw group (p = 0.456). Patients with preoperative thrombocytopenia had a transfusion rate of 0.5 ± 0.71 units atient compared to 0.04 ± 0.19 units atient in patients with normal platelet levels (p 0.005). The differences in blood loss observed between cannulated and solid pedicle screws are non-significant overall. The largest predictor for need of transfusion was pre-operative thrombocytopenia, regardless of the type of screw used.
Location: United States of America
No related grants have been discovered for Yansong Miao.