ORCID Profile
0000-0003-0904-976X
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Publisher: F1000 Research Ltd
Date: 02-08-2023
DOI: 10.12688/F1000RESEARCH.134798.1
Abstract: Background : Access to s le-level metadata is important when selecting public metagenomic sequencing datasets for reuse in new biological analyses. The Standards, Precautions, and Advances in Ancient Metagenomics community (SPAAM, spaam-community.github.io) has previously published AncientMetagenomeDir, a collection of curated and standardised s le metadata tables for metagenomic and microbial genome datasets generated from ancient s les. However, while s le-level information is useful for identifying relevant s les for inclusion in new projects, Next Generation Sequencing (NGS) library construction and sequencing metadata are also essential for appropriately reprocessing ancient metagenomic data. Currently, recovering information for downloading and preparing such data is difficult when laboratory and bioinformatic metadata is heterogeneously recorded in prose-based publications. Methods : Through a series of community-based hackathon events, AncientMetagenomeDir was updated to provide standardised library-level metadata of existing and new ancient metagenomic s les. In tandem, the companion tool 'AMDirT' was developed to facilitate automated metadata curation and data validation, as well as rapid data filtering and downloading. Results : AncientMetagenomeDir was extended to include standardised metadata of over 5000 ancient metagenomic libraries. The companion tool 'AMDirT' provides both graphical- and command-line interface based access to such metadata for users from a wide range of computational backgrounds. We also report on errors with metadata reporting that appear to commonly occur during data upload and provide suggestions on how to improve the quality of data sharing by the community. Conclusions : Together, both standardised metadata and tooling will help towards easier incorporation and reuse of public ancient metagenomic datasets into future analyses.
Publisher: Elsevier BV
Date: 12-2021
Location: Germany
No related grants have been discovered for Mohamed Sarhan.